Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	9,028	C→T	58.1%	S264F (TCT→TTT) ‡	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	9,028	0	C	T	58.1%	14.7 / 33.5	31	S264F (TCT→TTT) ‡	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base C (3/10);  new base T (10/8);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAA  >  CP009273/8883‑9161                                                                                                                                                  |                                                                                                                                      cACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTac                                                                                                                                   <  2:35894/150‑1 (MQ=255)           aaaCCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAg                                                                                                                         >  2:72400/1‑150 (MQ=255)                       tGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGt                                                                                                              <  2:167824/149‑1 (MQ=255)                         ggCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGACCGTAAGCTGTCTTCCTCCGCCGCAGTGAAAGCGCCTcc                                                                                                            <  2:53826/149‑1 (MQ=255)                           cgcAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTTCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCg                                                                                                           >  2:125500/1‑148 (MQ=255)                             cAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGgagc                                                                                                       >  1:157288/1‑150 (MQ=255)                              aaGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGagcg                                                                                                      <  1:114722/150‑1 (MQ=255)                               aGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGagcg                                                                                                      >  1:54782/1‑149 (MQ=255)                                 cTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAt                                                                                                   >  2:26170/1‑150 (MQ=255)                                    ccGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATc                                                                                                  <  1:160757/148‑1 (MQ=255)                                         acgttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGaa                                                                                            <  2:76814/144‑2 (MQ=255)                                          cgttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATc                                                                                          <  2:176142/146‑1 (MQ=255)                                            tCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCg                                                                                        <  1:5890/150‑1 (MQ=255)                                              ggCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCCTCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGCAGGGGCTATCGAACGTAAACTGTCCTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAg                                                                                      >  2:159742/1‑150 (MQ=255)                                                     ttCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCtgt                                                                               >  1:92404/1‑150 (MQ=255)                                                       cTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCtgtg                                                                              <  1:139394/149‑1 (MQ=255)                                                                         gACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATcc                                                            >  2:173408/1‑149 (MQ=255)                                                                         gACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATcc                                                            >  1:10300/1‑149 (MQ=255)                                                                          aCCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCaa                                                          <  2:122902/150‑1 (MQ=255)                                                                                      tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                              <  1:14118/150‑3 (MQ=255)                                                                                           ccGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATccga                                                          >  1:110610/1‑131 (MQ=255)                                                                                                     tgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTa                               >  1:73313/1‑150 (MQ=255)                                                                                                        aaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATcc                            <  1:26170/150‑1 (MQ=255)                                                                                                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                     <  2:112853/143‑1 (MQ=255)                                                                                                                 cGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTg                   <  1:147552/150‑1 (MQ=255)                                                                                                                    aaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGcga                >  1:99271/3‑148 (MQ=255)                                                                                                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc              <  1:134147/150‑2 (MQ=255)                                                                                                                         gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc           >  2:123044/1‑150 (MQ=255)                                                                                                                              ggCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGa      <  1:72400/150‑1 (MQ=255)                                                                                                                               gCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaa     <  1:125336/150‑1 (MQ=255)                                                                                                                                   tCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaaa  <  1:145340/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                      CACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAA  >  CP009273/8883‑9161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑