Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,484,919	C→G	100%	pseudogene (52/750 nt)	gapC ←	pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,484,919	0	C	G	100.0%	57.2 / NA	18	pseudogene (52/750 nt)	gapC	pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/9);  total (9/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGAATCTTCCGTAAAATCAACGCTCCAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTATGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCACAGTG  >  CP009273/1484778‑1485062                                                                                                                                              |                                                                                                                                                gTGAATCTTCCGTAAAATCAACGCTCCAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCa                                                                                                                                         <  2:161603/150‑1 (MQ=255)     aTCTTCCGTAAAATCAACGCTCCAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTc                                                                                                                                      >  2:169223/1‑149 (MQ=255)          ccGTAAAATCAACGCTCCAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGAcc                                                                                                                                  <  1:169136/148‑1 (MQ=255)             tAAAATCAACGCTCCAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATAc                                                                                                                             <  2:72980/150‑1 (MQ=255)               aaaTCAACGCTCCAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGa                                                                                                                           <  2:131135/150‑1 (MQ=255)                           cAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTa                                                                                                               <  1:193468/150‑1 (MQ=255)                                  aTGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCaa                                                                                                        >  1:182316/1‑150 (MQ=255)                                         gTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACt                                                                                                 >  1:109796/1‑150 (MQ=255)                                              ttGAATCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATgg                                                                                            >  1:182768/1‑150 (MQ=255)                                                  aTCATGTTTCAGCAGGTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACATCAGCCGACCGATACGACCAAAACAGTTAATACCAACTTTACTCATGGtttt                                                                                        >  1:160239/1‑150 (MQ=255)                                                                ggTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAAc                                                                          <  2:146557/150‑1 (MQ=255)                                                                ggTAGGCGAGAATTTTTGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTAGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAAc                                                                          >  2:134233/1‑150 (MQ=255)                                                                               ttGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAAtt                                                           >  1:180436/2‑150 (MQ=255)                                                                                          agaTCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTc                                                  >  2:123658/1‑148 (MQ=255)                                                                                                                              aCTTCAAGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAGGCTTAGACCAGGATGGCGGGATGGGCAATCTCCAt            <  2:12844/150‑1 (MQ=255)                                                                                                                                   aaGTAATCGACGCAACTCCAGTCGACCGATACGACCAAAACCGTTAATACCAACTATACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAGGCTTAGACCAGGATGGCGGGATGGGCAACCTCCATtctc        <  2:57102/149‑1 (MQ=255)                                                                                                                                    aGTAATCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAGGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTcac      <  2:109796/150‑1 (MQ=255)                                                                                                                                         tCGACGCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAGGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCACAGTg  >  2:61156/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                GTGAATCTTCCGTAAAATCAACGCTCCAGGGGAATGGTCCGTAGTTTGAATCATGTTTCAGCAGGTAGGCGAGAATTTATGGGGAAGTGAGATCATTAATAGCGACAACGTCTATGTTGCTTTTGACTTCAAGTAATCGACCCAACACCAGTCGACCGATACGACCAAAACCGTTAATACCAACTTTACTCATGGTTTTCTCCTGTCAGGAACGTTCGGATGAAAATTGATCCTTTCCAAGCTTAGACCAGGATGGCGGGATGGGCAATCTCCATTCTCACAGTG  >  CP009273/1484778‑1485062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑