| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,909,758 | C→A | 100% | E12* (GAA→TAA) | proQ ← | RNA chaperone, putative ProP translation regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,909,758 | 0 | C | A | 87.5% | 17.9 / ‑2.7 | 8 | E12* (GAA→TAA) | proQ | RNA chaperone, putative ProP translation regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (4/3); total (4/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTA > CP009273/1909612‑1909879 | aGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTActtatt > 2:73897/1‑149 (MQ=255)aGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTAttt < 2:58086/150‑1 (MQ=255) gCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGtc < 2:152208/149‑2 (MQ=255) ttCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTcc < 1:195500/150‑1 (MQ=255) aCCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAgcg > 1:68377/1‑148 (MQ=255) cGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAgg > 2:97159/1‑150 (MQ=255) tGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGc > 1:194021/1‑149 (MQ=255) ggAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACg < 1:97159/150‑1 (MQ=255) aaaCGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTa > 1:190022/1‑150 (MQ=255) | AGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTA > CP009273/1909612‑1909879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |