Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,074,575 C→G 33.9% P140P (CCG→CCC)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,074,5750CG33.9% 69.6 / 67.1 62P140P (CCG→CCC) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (24/17);  new base G (10/11);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

TCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACG  >  CP009273/3074426‑3074724
                                                                                                                                                     |                                                                                                                                                     
tCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc                                                                                                                                                       <  2:98425/150‑1 (MQ=255)
tCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc                                                                                                                                                       >  1:69388/1‑150 (MQ=255)
 cGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCg                                                                                                                                                      >  2:60793/1‑150 (MQ=255)
    tAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGAc                                                                                                                                                   >  2:151025/1‑150 (MQ=255)
     aGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACg                                                                                                                                                  >  2:73896/1‑150 (MQ=255)
                tGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggc                                                                                                                                       >  1:75445/1‑148 (MQ=255)
                 gtcgtcGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggc                                                                                                                                       <  1:194233/149‑3 (MQ=255)
                 gTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTgcgc                                                                                                                                      >  2:157359/1‑150 (MQ=255)
                  tcgtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggccc                                                                                                                                     <  2:165289/147‑5 (MQ=255)
                    gtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggcccg                                                                                                                                    <  1:80840/148‑6 (MQ=255)
                       gTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAg                                                                                                                                >  2:176703/1‑150 (MQ=255)
                        tagacggCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGc                                                                                                                               <  1:139838/144‑1 (MQ=255)
                           acggCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcg                                                                                                                            <  2:94201/147‑3 (MQ=255)
                            aTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGttt                                                                                                                           >  2:99941/1‑150 (MQ=255)
                             ggCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCcgcgcgcg                                                                                                                          >  1:109079/2‑146 (MQ=255)
                              gCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGttttt                                                                                                                         >  2:155328/1‑150 (MQ=255)
                                aaTCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcgcgttc                                                                                                                       <  2:39535/150‑8 (MQ=255)
                                aaTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTc                                                                                                                       >  2:20401/1‑150 (MQ=255)
                                    aGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGc                                                                                                                    <  2:190504/149‑1 (MQ=255)
                                      tttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAAt                                                                                                                 >  2:11163/1‑150 (MQ=255)
                                      tttACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAAt                                                                                                                 <  1:70251/150‑1 (MQ=255)
                                        tACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATc                                                                                                                <  2:11936/149‑1 (MQ=255)
                                           ccAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCa                                                                                                            >  1:174197/1‑150 (MQ=255)
                                            cAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCa                                                                                                            >  2:117660/1‑149 (MQ=255)
                                                    gCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAACCTGCGCCGCCAGCGTTTTTTCTGCCATCGCCATACCGACTg                                                                                                   <  2:158364/150‑1 (MQ=255)
                                                        aCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGtt                                                                                               >  2:37575/1‑150 (MQ=255)
                                                                  gagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAccc                                                                                     <  1:42808/150‑1 (MQ=255)
                                                                   agCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCt                                                                                    >  1:145666/1‑150 (MQ=255)
                                                                    gCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTg                                                                                   <  1:73896/150‑1 (MQ=255)
                                                                     cAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGa                                                                                  >  2:155057/1‑150 (MQ=255)
                                                                      aagcTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGAc                                                                                 >  1:122710/4‑150 (MQ=255)
                                                                       agcTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGAcc                                                                                >  1:8257/3‑150 (MQ=255)
                                                                        aCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc                                                                                >  1:140509/1‑149 (MQ=255)
                                                                         cTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGAccta                                                                              <  2:75445/150‑3 (MQ=255)
                                                                         cTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGAccta                                                                              <  2:182026/150‑3 (MQ=255)
                                                                                cgagaTGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTcc                                                                       <  1:4254/149‑1 (MQ=255)
                                                                                 gagaTGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCg                                                                      >  2:17983/1‑150 (MQ=255)
                                                                                   gaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggt                                                                    >  1:175010/1‑150 (MQ=255)
                                                                                            cattaagCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTc                                                           <  2:92247/144‑1 (MQ=255)
                                                                                            catcatGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGACCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTc                                                           >  2:169259/1‑150 (MQ=255)
                                                                                                aagCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCgacg                                                       >  1:122517/3‑149 (MQ=255)
                                                                                                aagCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCgac                                                        <  1:149637/147‑1 (MQ=255)
                                                                                                  gCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCacac                                                      >  2:141851/1‑149 (MQ=255)
                                                                                                   cAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCCGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCGACGccc                                                    >  2:71120/1‑149 (MQ=255)
                                                                                                   cAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCGACGccc                                                    >  1:5291/1‑149 (MQ=255)
                                                                                                        gTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCCGCGCGCGTTCGGCTATCGCGAAACCCACCGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCGACGcccggcg                                                >  1:69933/1‑144 (MQ=255)
                                                                                                         tCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtg                                              <  2:175010/150‑1 (MQ=255)
                                                                                                         tCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtg                                              <  2:50836/150‑1 (MQ=255)
                                                                                                           gCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtgt                                             >  2:125804/1‑149 (MQ=255)
                                                                                                            cccATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTaa                                           >  2:164962/1‑150 (MQ=255)
                                                                                                             ccATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAc                                          <  1:618/150‑1 (MQ=255)
                                                                                                              cATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAcc                                         >  1:103261/1‑150 (MQ=255)
                                                                                                               aTGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAcc                                         >  1:87668/1‑149 (MQ=255)
                                                                                                                 gAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCAc                                      >  2:170237/1‑150 (MQ=255)
                                                                                                                 gAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCAc                                      >  1:75752/1‑150 (MQ=255)
                                                                                                                         aGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGt                              >  2:66394/1‑150 (MQ=255)
                                                                                                                          ggTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGt                              <  1:99941/149‑1 (MQ=255)
                                                                                                                              tAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAcc                          <  1:25703/149‑1 (MQ=255)
                                                                                                                                   gTCGACAATGTCGTGGCCCGGACGGTTAAGCTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATGCCCTGACCCAGCGGACCGGTGGTGGTGTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt                    <  2:41066/150‑1 (MQ=255)
                                                                                                                                      gACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGtttt                 <  1:155057/150‑1 (MQ=255)
                                                                                                                                         aaTGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTaga              <  1:116709/150‑1 (MQ=255)
                                                                                                                                           tGTCGTGGCCCGGGCGGTTAAACTGCGCCGCCATTTTTTTTTCTACAGTCGCCATACCGACTGCGTTGGCAATCCCCTGACCCAGCGCGCCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGt            <  1:157359/150‑1 (MQ=255)
                                                                                                                                           tGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGt            <  2:103261/150‑1 (MQ=255)
                                                                                                                                                  gCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTg     <  2:145666/150‑1 (MQ=255)
                                                                                                                                                     cGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACg  <  1:11163/150‑1 (MQ=255)
                                                                                                                                                     cGGAAGGGTAAACGGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACg  <  1:117660/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACG  >  CP009273/3074426‑3074724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑