| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,729,296 | A→C | 100% | K320Q (AAG→CAG) | xylR → | xylose divergent operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,729,296 | 0 | A | C | 100.0% | 42.6 / NA | 13 | K320Q (AAG→CAG) | xylR | xylose divergent operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/4); total (9/4) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTT > CP009273/3729154‑3729441 | tGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGttt > 1:113884/1‑150 (MQ=255) aCGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTa > 1:95658/1‑149 (MQ=255) ggCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTa > 2:24125/1‑147 (MQ=255) ctccctGCCCGCCCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTGAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAAccat < 2:61197/150‑1 (MQ=255) gTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGcc > 1:129375/1‑150 (MQ=255) gCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGgaga > 1:90229/1‑149 (MQ=255) gCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGgaga > 1:92807/1‑149 (MQ=255) aaGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAacca < 1:53219/150‑1 (MQ=255) gggATTAAAGCGGATCAGGTACTGGATGCGGCCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAaccacc > 1:106976/1‑150 (MQ=255) aaGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCg < 2:113884/149‑1 (MQ=255) tCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAAt > 1:10906/1‑148 (MQ=255) tGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCg > 2:74973/1‑149 (MQ=255) cgcTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCg < 2:95658/150‑1 (MQ=255) gagcAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATAttt < 1:89383/149‑1 (MQ=255) | TGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTT > CP009273/3729154‑3729441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |