Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 8,825 T→A A196A (GCT→GCA)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,8250TA80.5% 1330.2 / inf 576A196A (GCT→GCA) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (51/61);  new base A (218/243);  total (269/307)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-02

Only 100 of 577 total aligned reads displayed.

GAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCAC  >  CP009273/8676‑8973
                                                                                                                                                     |                                                                                                                                                     
gAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCt                                                                                                                                                       >  1:1978960/1‑150 (MQ=255)
  aaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCAcc                                                                                                                                                     >  1:2308235/1‑150 (MQ=255)
  aaaaGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTcc                                                                                                                                                     <  2:1257104/150‑1 (MQ=255)
   aaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCg                                                                                                                                                    >  2:777372/1‑150 (MQ=255)
    aagaagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCgg                                                                                                                                                   <  2:1409406/150‑1 (MQ=255)
       aagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                                                                                                                <  1:334931/150‑1 (MQ=255)
       aagGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCag                                                                                                                                                <  2:260325/150‑1 (MQ=255)
        agGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCaga                                                                                                                                               <  2:62977/150‑1 (MQ=255)
        agGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                                                                                                                >  2:2404709/1‑149 (MQ=255)
        agGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                                                                                                                >  2:704541/1‑149 (MQ=255)
          gCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCagaag                                                                                                                                             <  1:1778618/150‑1 (MQ=255)
          gCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCag                                                                                                                                                >  2:1875229/1‑147 (MQ=255)
             ttaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATc                                                                                                                                          >  2:1332242/3‑150 (MQ=255)
             ttaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATc                                                                                                                                          >  2:2114123/3‑150 (MQ=255)
             ttaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATc                                                                                                                                          <  1:288760/148‑1 (MQ=255)
              taaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATcc                                                                                                                                         >  1:971778/2‑150 (MQ=255)
               aaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATcc                                                                                                                                         <  1:2272735/149‑1 (MQ=255)
               aaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATcc                                                                                                                                         <  2:1495553/149‑1 (MQ=255)
                 cTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCggg                                                                                                                                      >  2:2203218/1‑150 (MQ=255)
                    tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGc                                                                                                                                     <  1:385101/148‑1 (MQ=255)
                    tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGt                                                                                                                                   <  2:47351/150‑1 (MQ=255)
                     aaCCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTg                                                                                                                                  >  2:291509/1‑150 (MQ=255)
                       ccTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGt                                                                                                                                >  1:1821590/1‑150 (MQ=255)
                         tGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGttt                                                                                                                              >  1:916572/1‑150 (MQ=255)
                         tGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGttt                                                                                                                              >  2:856632/1‑150 (MQ=255)
                             gctgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTccgt                                                                                                                          >  2:523443/2‑147 (MQ=255)
                              ctgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtc                                                                                                                         <  1:2143031/150‑2 (MQ=255)
                               tgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGt                                                                                                                          >  2:821539/1‑148 (MQ=255)
                                 ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtca                                                                                                                        <  2:1673657/148‑3 (MQ=255)
                                 ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATCTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTccgtcag                                                                                                                       <  2:129678/149‑7 (MQ=255)
                                    ttCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcagcgaa                                                                                                                   <  1:756353/150‑8 (MQ=255)
                                             gCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAg                                                                                                         >  1:1652556/1‑150 (MQ=255)
                                                 aGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGtat                                                                                                      <  1:2388689/149‑2 (MQ=255)
                                                  ggCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGtatta                                                                                                    >  1:839157/1‑150 (MQ=255)
                                                  ggCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGtatta                                                                                                    >  2:2169790/1‑147 (MQ=255)
                                                       gcgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACaag                                                                                                >  2:1229660/3‑148 (MQ=255)
                                                       gcgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACaag                                                                                                <  2:1076214/147‑2 (MQ=255)
                                                        cgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACaag                                                                                                >  2:102215/2‑147 (MQ=255)
                                                         gCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACaagcag                                                                                             >  1:811400/1‑146 (MQ=255)
                                                             gcgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCAc                                                                                          <  2:1948748/144‑1 (MQ=255)
                                                                   aaGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                    >  1:1851550/1‑149 (MQ=255)
                                                                    aGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATg                                                                                   >  2:402869/1‑149 (MQ=255)
                                                                     gCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGa                                                                                  >  2:341025/1‑149 (MQ=255)
                                                                        ggCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAAcc                                                                              <  2:1821590/150‑1 (MQ=255)
                                                                         gagccttCCAGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACCAGAAAGAGTACGCGCCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACGAGCAGCACGGCTATTAAACCg                                                                             <  2:1574691/145‑1 (MQ=255)
                                                                          cGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGt                                                                            <  2:2310445/150‑1 (MQ=255)
                                                                               tCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTa                                                                       >  2:2249827/1‑150 (MQ=255)
                                                                                ccTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgtg                                                                        <  1:2212268/148‑2 (MQ=255)
                                                                                 cTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATg                                                                     >  1:1226694/1‑150 (MQ=255)
                                                                                   gATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGATATGAAACCGTCGTGATggg                                                                   >  2:1605678/1‑150 (MQ=255)
                                                                                    atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATggg                                                                   <  1:124084/146‑1 (MQ=255)
                                                                                    atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATggg                                                                   <  1:1005207/146‑1 (MQ=255)
                                                                                    aTCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATggg                                                                   <  2:673486/149‑1 (MQ=255)
                                                                                     tttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgcg                                                                 <  1:24537/148‑1 (MQ=255)
                                                                                     tttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgcg                                                                 <  2:1736686/148‑1 (MQ=255)
                                                                                         gCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGgcg                                                                 <  1:778365/146‑1 (MQ=255)
                                                                                           cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                                           >  2:2004163/1‑150 (MQ=255)
                                                                                             tttGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTc                                                         <  1:1470222/150‑1 (MQ=255)
                                                                                             ttcgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTc                                                         >  2:1841990/4‑150 (MQ=255)
                                                                                               cgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGt                                                                            >  2:1916379/2‑129 (MQ=255)
                                                                                               cgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTcc                                                        <  1:5307/148‑1 (MQ=255)
                                                                                                gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                                       >  1:192593/1‑149 (MQ=255)
                                                                                                 ttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGt                                                      >  2:382885/1‑149 (MQ=255)
                                                                                                  tgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTaa                                                    >  1:1327227/1‑150 (MQ=255)
                                                                                                   ggCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTaa                                                    <  1:1547960/149‑1 (MQ=255)
                                                                                                    gCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACa                                                  <  2:977283/150‑1 (MQ=255)
                                                                                                    gCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAc                                                   <  1:2032622/149‑1 (MQ=255)
                                                                                                    gCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAc                                                   <  1:1400255/149‑1 (MQ=255)
                                                                                                           tCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCg                                            >  1:1521028/1‑149 (MQ=255)
                                                                                                            ctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGaa                                          <  1:576241/147‑1 (MQ=255)
                                                                                                              tGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAAt                                        >  1:2344266/1‑150 (MQ=255)
                                                                                                               gACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAAtt                                       <  2:1541524/150‑1 (MQ=255)
                                                                                                               gACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAAtt                                       <  2:1177192/150‑1 (MQ=255)
                                                                                                                 cTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCt                                     <  1:1374201/150‑1 (MQ=255)
                                                                                                                    gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTgg                                   <  1:310870/149‑1 (MQ=255)
                                                                                                                    gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGa                                  <  1:1303966/150‑1 (MQ=255)
                                                                                                                     tACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGaa                                 >  1:1089379/1‑150 (MQ=255)
                                                                                                                       cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc                                <  1:428261/149‑1 (MQ=255)
                                                                                                                       cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt                               >  2:749451/1‑150 (MQ=255)
                                                                                                                         aaGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTgg                             <  1:1875229/150‑1 (MQ=255)
                                                                                                                         aaGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTgg                             >  1:1694392/1‑150 (MQ=255)
                                                                                                                           gCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCa                           <  2:1436971/150‑1 (MQ=255)
                                                                                                                            ccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctg                          >  2:424765/6‑148 (MQ=255)
                                                                                                                            ccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctg                          >  1:161426/6‑148 (MQ=255)
                                                                                                                             caacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctgg                         >  2:164147/5‑147 (MQ=255)
                                                                                                                              aacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACtggctggc                        >  1:1183953/4‑150 (MQ=255)
                                                                                                                               aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCt                       <  2:112807/150‑1 (MQ=255)
                                                                                                                                 aCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctgt                     <  1:691237/150‑2 (MQ=255)
                                                                                                                                      caagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCg                >  1:497907/2‑150 (MQ=255)
                                                                                                                                      caagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCg                <  1:2082004/149‑1 (MQ=255)
                                                                                                                                       aagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgc               <  1:1146781/150‑2 (MQ=255)
                                                                                                                                        agaaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGt               <  2:2349386/149‑1 (MQ=255)
                                                                                                                                          aaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTg            <  1:1964664/150‑1 (MQ=255)
                                                                                                                                          aaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTg            <  1:656966/150‑1 (MQ=255)
                                                                                                                                             gagTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGAcc         <  1:2380355/150‑1 (MQ=255)
                                                                                                                                              agTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCa        <  2:2057436/150‑1 (MQ=255)
                                                                                                                                               gTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCAt       >  1:738443/1‑150 (MQ=255)
                                                                                                                                                tACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATc      >  2:1464734/1‑150 (MQ=255)
                                                                                                                                                 aCGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCg     >  2:908577/1‑150 (MQ=255)
                                                                                                                                                    cTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCAc  <  2:1380956/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCAC  >  CP009273/8676‑8973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑