Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 8,868 C→G Q211E (CAG→GAG)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,8680CG77.7% 1340.4 / inf 556Q211E (CAG→GAG) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base C (63/61);  new base G (201/231);  total (264/292)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 560 total aligned reads displayed.

CGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAA  >  CP009273/8720‑9017
                                                                                                                                                     |                                                                                                                                                     
 gCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAg                                                                                                                                                     >  2:1562791/1‑150 (MQ=255)
     aGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGtat                                                                                                                                                  >  1:2386278/1‑148 (MQ=255)
     aGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGtat                                                                                                                                                  <  1:771925/149‑2 (MQ=255)
      ggCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGtatta                                                                                                                                                >  2:2169790/1‑147 (MQ=255)
       gCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGtatta                                                                                                                                                >  2:1678552/1‑149 (MQ=255)
        cTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGtattac                                                                                                                                               >  2:1186258/1‑149 (MQ=255)
            cgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACaagca                                                                                                                                          <  2:333946/149‑4 (MQ=255)
            cgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACaag                                                                                                                                            >  2:102215/2‑147 (MQ=255)
                 gcgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGATAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGCTTCCGT‑CAGCGAAACCTATGAGTCTTACAAACAGCAc                                                                                                                                      <  2:2028545/144‑1 (MQ=255)
                  cgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACgg                                                                                                                                    >  1:1773934/5‑150 (MQ=255)
                   gccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACggc                                                                                                                                   <  1:2284240/147‑2 (MQ=255)
                   gccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACggc                                                                                                                                   >  1:83856/4‑149 (MQ=255)
                    ccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACggc                                                                                                                                   >  2:1356131/3‑148 (MQ=255)
                    cGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGtt                                                                                                                                  <  1:508085/150‑1 (MQ=255)
                      gAAGTGGGCTTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                                                                <  1:1319026/150‑1 (MQ=255)
                      gAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                                                                >  1:2000498/1‑150 (MQ=255)
                      gAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                                                                <  2:935662/150‑1 (MQ=255)
                        aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGa                                                                                                                              <  1:1831569/150‑1 (MQ=255)
                        aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATg                                                                                                                               >  2:289834/1‑149 (MQ=255)
                        aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGa                                                                                                                              <  1:1124859/150‑1 (MQ=255)
                              cGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGt                                                                                                                        <  1:792249/150‑1 (MQ=255)
                               gtgtTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTg                                                                                                                       <  1:1473747/150‑1 (MQ=255)
                               gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                                                                                       <  1:701121/147‑2 (MQ=255)
                               gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                                                                                       >  1:2316572/4‑149 (MQ=255)
                               gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                                                                                       <  1:1365571/147‑2 (MQ=255)
                               gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                                                                                       <  2:1077195/147‑2 (MQ=255)
                                tcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACTAGCAGCACGGCTATGAAACCGtcg                                                                                                                      <  1:1572553/148‑3 (MQ=255)
                                    ccTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGTCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTCCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgtga                                                                                                                   <  1:2112232/149‑3 (MQ=255)
                                     cTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtcgtgat                                                                                                                  <  2:1723291/149‑7 (MQ=255)
                                     cTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATg                                                                                                                 >  1:287596/1‑150 (MQ=255)
                                       gATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATGTATGAGTATTACAAGCAGCATGGCTATGAAACCGTCGTGATggg                                                                                                               >  2:1284685/1‑150 (MQ=255)
                                       gATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGATATGAAACCGTCGTGATggg                                                                                                               >  2:1605678/1‑150 (MQ=255)
                                        atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATggg                                                                                                               <  1:1005207/146‑1 (MQ=255)
                                         tttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgcg                                                                                                             <  1:2404709/148‑1 (MQ=255)
                                          ttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgcg                                                                                                             >  2:558951/2‑149 (MQ=255)
                                             gCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGgc                                                                                                              <  2:1978960/145‑1 (MQ=255)
                                              ccGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGc                                                                                                        >  2:1310748/1‑150 (MQ=255)
                                               cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                                                                                       >  2:656966/1‑150 (MQ=255)
                                               cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                                                                                       <  2:404807/150‑1 (MQ=255)
                                                 ttcgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTc                                                                                                     >  1:673036/4‑150 (MQ=255)
                                                    gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                                                                                       <  1:1916379/129‑2 (MQ=255)
                                                       ggCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTaa                                                                                                <  2:1383229/149‑1 (MQ=255)
                                                        gCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGc                                                                                                        <  1:127715/140‑1 (MQ=255)
                                                        gCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACa                                                                                              <  2:977283/150‑1 (MQ=255)
                                                        gCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTCATGGGCGCAAGCTTCCGTAACa                                                                                              <  1:2072991/150‑1 (MQ=255)
                                                         ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                                                                                             >  1:1693420/1‑148 (MQ=255)
                                                          cGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgtt                                                                                            >  2:728313/1‑147 (MQ=255)
                                                           gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                                                                                           >  2:682368/1‑146 (MQ=255)
                                                           gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                                                                                           <  1:560576/150‑5 (MQ=255)
                                                             atcctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGc                                                                                         >  2:1639921/7‑150 (MQ=255)
                                                              ttCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGc                                                                                         <  1:2199028/149‑1 (MQ=255)
                                                                   gACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAAt                                                                                    <  1:1219506/149‑1 (MQ=255)
                                                                   gACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGaaa                                                                                     <  2:509955/148‑1 (MQ=255)
                                                                   gACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAAtt                                                                                   <  2:1541524/150‑1 (MQ=255)
                                                                    aCTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTc                                                                                  >  2:243459/1‑150 (MQ=255)
                                                                     cTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAGCCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCt                                                                                 >  2:1819950/1‑150 (MQ=255)
                                                                        gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTgg                                                                               <  1:310870/149‑1 (MQ=255)
                                                                        gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTg                                                                                <  2:872853/148‑1 (MQ=255)
                                                                        gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTACCGTAACGTTGGCGAAATTCTgg                                                                               <  1:613942/149‑1 (MQ=255)
                                                                           cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc                                                                            <  1:428261/149‑1 (MQ=255)
                                                                            aaaGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTg                                                                          <  1:747497/150‑1 (MQ=255)
                                                                             aaGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTgg                                                                         >  2:1488791/1‑150 (MQ=255)
                                                                             aaGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTgg                                                                         <  1:1332242/150‑1 (MQ=255)
                                                                                  aaTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGc                                                                    <  2:765724/150‑1 (MQ=255)
                                                                                   aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCt                                                                   <  2:112807/150‑1 (MQ=255)
                                                                                     aCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctgt                                                                 >  2:1830751/1‑149 (MQ=255)
                                                                                     aCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctgt                                                                 <  2:2201321/150‑2 (MQ=255)
                                                                                       cGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctgtga                                                               >  2:1869489/1‑147 (MQ=255)
                                                                                       cGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGa                                                               >  2:2066207/1‑150 (MQ=255)
                                                                                        gacaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAc                                                              >  2:1436464/4‑150 (MQ=255)
                                                                                         acaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAcc                                                             >  2:2128049/3‑150 (MQ=255)
                                                                                         acaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAcc                                                             <  1:1869489/148‑1 (MQ=255)
                                                                                          caagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCg                                                            <  1:1649196/149‑1 (MQ=255)
                                                                                            agaaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGt                                                           <  2:2349386/149‑1 (MQ=255)
                                                                                            agaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgcc                                                          >  1:1160776/1‑148 (MQ=255)
                                                                                             gaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgcct                                                         >  1:2360915/1‑147 (MQ=255)
                                                                                              aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTg                                                        <  1:37840/150‑1 (MQ=255)
                                                                                              aaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgcct                                                         >  2:794038/1‑146 (MQ=255)
                                                                                              aaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTg                                                        <  1:1964664/150‑1 (MQ=255)
                                                                                                      cGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGc                                                >  2:1247216/1‑150 (MQ=255)
                                                                                                         accGGCAGAAGATCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                             <  2:227349/149‑1 (MQ=255)
                                                                                                         accGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                             <  1:2239119/149‑1 (MQ=255)
                                                                                                          ccGGCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCg                                            <  2:453194/150‑1 (MQ=255)
                                                                                                             gCAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcg                                         <  2:383632/150‑2 (MQ=255)
                                                                                                              cAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcgc                                        >  2:2299992/1‑148 (MQ=255)
                                                                                                              cAGAAGATCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcgc                                        >  2:1461923/1‑148 (MQ=255)
                                                                                                               agaagaTCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcgct                                       >  1:1070092/1‑147 (MQ=255)
                                                                                                                gaagaTCCGGGCGTGGTTTCCGTCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGctg                                      >  2:2410027/1‑150 (MQ=255)
                                                                                                                 aagaTCCGGGCGTGGTTTCCGT‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt                                                                           >  1:1036073/1‑112 (MQ=255)
                                                                                                                          gCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGc                            >  1:95208/1‑150 (MQ=255)
                                                                                                                            gTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTg                          >  2:2314047/1‑150 (MQ=255)
                                                                                                                             tGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTgg                         >  1:1427676/1‑150 (MQ=255)
                                                                                                                                      gtcagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGc                 <  1:1186258/144‑1 (MQ=255)
                                                                                                                                         tCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGa               <  2:1931039/149‑1 (MQ=255)
                                                                                                                                          gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg             <  1:470578/148‑1 (MQ=255)
                                                                                                                                           cgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAgg            <  2:1773934/149‑1 (MQ=255)
                                                                                                                                             aaaTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGCAACGGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGTAAGCGAAggcg          >  2:1911671/1‑148 (MQ=255)
                                                                                                                                             aaaTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAgg            <  2:345640/148‑1 (MQ=255)
                                                                                                                                             aaaTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAgg            <  1:863826/148‑1 (MQ=255)
                                                                                                                                               aTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgcc        <  1:1517037/150‑2 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAA  >  CP009273/8720‑9017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑