Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 8,873 C→T Y212Y (TAC→TAT)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,8730CT77.4% 1113.4 / inf 535Y212Y (TAC→TAT) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base C (62/59);  new base T (192/222);  total (254/281)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

Only 100 of 548 total aligned reads displayed.

AGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTA  >  CP009273/8725‑9021
                                                                                                                                                      |                                                                                                                                                    
 ggCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGtatta                                                                                                                                                    >  1:839157/1‑150 (MQ=255)
      gcgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACaag                                                                                                                                                <  2:1076214/147‑2 (MQ=255)
       tGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACaaa                                                                                                                                                >  2:863826/1‑148 (MQ=255)
       cgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACaag                                                                                                                                                >  2:102215/2‑147 (MQ=255)
        gCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACaagcag                                                                                                                                             >  1:811400/1‑146 (MQ=255)
             cgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACgg                                                                                                                                        >  1:1773934/5‑150 (MQ=255)
              gccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACggc                                                                                                                                       <  2:1548316/147‑2 (MQ=255)
                ctaaGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACggcta                                                                                                                                     >  2:1509006/3‑147 (MQ=255)
                 gAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                                                                    >  1:2000498/1‑150 (MQ=255)
                 gAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                                                                    <  2:935662/150‑1 (MQ=255)
                  aaGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATg                                                                                                                                   <  2:1858035/150‑1 (MQ=255)
                   aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGa                                                                                                                                  <  1:1831569/150‑1 (MQ=255)
                   aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGa                                                                                                                                  <  1:1124859/150‑1 (MQ=255)
                    gCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCAc                                                                                                                                          <  2:2277179/141‑1 (MQ=255)
                       ggCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAAcc                                                                                                                              <  2:1821590/150‑1 (MQ=255)
                         cGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGt                                                                                                                            >  2:1591270/1‑150 (MQ=255)
                          gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                                                                                           >  1:2316572/4‑149 (MQ=255)
                          gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                                                                                           <  1:196641/147‑2 (MQ=255)
                           tcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGACACCGtcg                                                                                                                          >  1:2074598/3‑148 (MQ=255)
                              tCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgtga                                                                                                                       >  2:529941/1‑148 (MQ=255)
                               ccTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGTCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTCCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgtga                                                                                                                       <  1:2112232/149‑3 (MQ=255)
                               ccTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgtga                                                                                                                       <  1:2385190/149‑3 (MQ=255)
                                cTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATg                                                                                                                     >  1:287596/1‑150 (MQ=255)
                                   atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATggg                                                                                                                   <  1:1005207/146‑1 (MQ=255)
                                   aTCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATggg                                                                                                                   <  2:673486/149‑1 (MQ=255)
                                        gCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCaa                                                                                                              <  1:1270954/149‑1 (MQ=255)
                                        gCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAg                                                                                                             >  1:1152514/1‑150 (MQ=255)
                                        gCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAg                                                                                                             >  2:1180895/1‑150 (MQ=255)
                                          cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                                                                                           <  1:603527/150‑1 (MQ=255)
                                           gTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCtt                                                                                                          >  2:1745831/1‑150 (MQ=255)
                                           gTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCtt                                                                                                          >  2:2233582/1‑150 (MQ=255)
                                           gTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCtt                                                                                                          >  1:1646432/1‑150 (MQ=255)
                                            tttGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTc                                                                                                         <  1:1470222/150‑1 (MQ=255)
                                               gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGt                                                                                                      <  1:696714/150‑1 (MQ=255)
                                                  ggCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAg                                                                                                             >  2:127715/1‑140 (MQ=255)
                                                  ggCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTaa                                                                                                    <  1:1547960/149‑1 (MQ=255)
                                                   gCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAAc                                                                                                   <  1:1063760/149‑1 (MQ=255)
                                                    ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                                                                                                 >  1:1693420/1‑148 (MQ=255)
                                                     cGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgtt                                                                                                <  2:791755/150‑4 (MQ=255)
                                                     cGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgtt                                                                                                <  1:501146/150‑4 (MQ=255)
                                                      gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                                                                                               >  2:1477223/1‑146 (MQ=255)
                                                      gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                                                                                               <  1:560576/150‑5 (MQ=255)
                                                         ttCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGc                                                                                             <  1:2199028/149‑1 (MQ=255)
                                                           ctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGa                                                                                           <  2:1402858/146‑1 (MQ=255)
                                                                 tGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTg                                                                                    >  1:775874/1‑150 (MQ=255)
                                                                   gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTgg                                                                                   <  1:310870/149‑1 (MQ=255)
                                                                   gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGa                                                                                  <  1:875455/150‑1 (MQ=255)
                                                                   gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGa                                                                                  <  1:1303966/150‑1 (MQ=255)
                                                                    tACAAAGCCAACACCGACAAGAATGAGTACGCACCCGCAGACGAACCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGaa                                                                                 <  2:1892189/150‑1 (MQ=255)
                                                                      cAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAAc                                                                                <  2:1652556/149‑1 (MQ=255)
                                                                      cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt                                                                               >  2:749451/1‑150 (MQ=255)
                                                                       aaaGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTg                                                                              <  1:747497/150‑1 (MQ=255)
                                                                           ccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctg                                                                          >  1:190658/6‑148 (MQ=255)
                                                                           ccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctg                                                                          >  2:424765/6‑148 (MQ=255)
                                                                           ccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctg                                                                          >  2:335448/6‑148 (MQ=255)
                                                                                 ccGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctgtg                                                                    <  1:1410888/150‑3 (MQ=255)
                                                                                  cGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctgtga                                                                   >  1:670231/1‑147 (MQ=255)
                                                                                   gacaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAc                                                                  >  2:2314959/4‑150 (MQ=255)
                                                                                    acaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAcc                                                                 >  2:718896/3‑150 (MQ=255)
                                                                                    acaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAcc                                                                 <  1:1869489/148‑1 (MQ=255)
                                                                                     caagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCg                                                                >  2:396542/2‑150 (MQ=255)
                                                                                         aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTg                                                            <  1:37840/150‑1 (MQ=255)
                                                                                         aaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgcct                                                             >  2:1924261/1‑146 (MQ=255)
                                                                                                aCGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCg                                                     >  2:255515/1‑150 (MQ=255)
                                                                                                  gcaccGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcc                                                   <  2:2306655/147‑2 (MQ=255)
                                                                                                    cccGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                                 <  2:1340641/149‑1 (MQ=255)
                                                                                                    accGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                                 <  1:2239119/149‑1 (MQ=255)
                                                                                                    accGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                                 >  2:2155839/2‑147 (MQ=255)
                                                                                                    accGGCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                                 >  1:1185259/2‑150 (MQ=255)
                                                                                                      cGGCAGAAGATCCGGGCGTGGTTTCGG‑T‑ATCTGAAATCTAACGGTACTACAAAGAGCACGGTTATGATACCGCGGTTATGGGCGCAATCTTCCGCAACATCGGCGAAAATCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCAcc                                                 <  2:171575/148‑1 (MQ=255)
                                                                                                      cGGCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCgg                                               >  1:1331163/1‑150 (MQ=255)
                                                                                                       ggCAGAAGATCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGc                                              >  2:163205/1‑150 (MQ=255)
                                                                                                        gCAGAAGATCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcg                                             >  2:1947498/1‑149 (MQ=255)
                                                                                                          agaagaTCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCATGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcgct                                           >  1:1217391/1‑147 (MQ=255)
                                                                                                           gaagaTCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGctg                                          >  2:218120/1‑150 (MQ=255)
                                                                                                            aagaTCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt                                                                               >  1:1036073/1‑112 (MQ=255)
                                                                                                             agaTCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCgctgct                                        >  2:1289400/1‑150 (MQ=255)
                                                                                                               aTCCGGGCGTGGTTTCCG‑TCAGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGctgctg                                       >  2:2355575/1‑149 (MQ=255)
                                                                                                                tCCGGGCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGa                                      >  2:1809998/1‑149 (MQ=255)
                                                                                                                 ccGGGCGTGGTTTCCGTT‑AGC‑GAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcg                                             <  1:227996/141‑2 (MQ=255)
                                                                                                                   gggCGTGGTTTCCG‑T‑CAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGaaa                                    >  2:976007/1‑148 (MQ=255)
                                                                                                                     gCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGc                                >  1:95208/1‑150 (MQ=255)
                                                                                                                               tG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGCCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGagag                      >  1:1342851/1‑150 (MQ=255)
                                                                                                                                  t‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTACTGAAAGAGCTGGCGGAGAGc                     <  2:2050114/149‑1 (MQ=255)
                                                                                                                                  gtcagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGc                     >  2:627097/7‑150 (MQ=255)
                                                                                                                                     agcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGa                   <  1:418150/146‑1 (MQ=255)
                                                                                                                                      gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg                 <  1:470578/148‑1 (MQ=255)
                                                                                                                                      gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg                 <  1:2355575/148‑1 (MQ=255)
                                                                                                                                      cTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAg                 >  2:295465/1‑150 (MQ=255)
                                                                                                                                        gAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAggg               >  2:1119466/1‑150 (MQ=255)
                                                                                                                                         aaaTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAggcg              >  2:14507/1‑148 (MQ=255)
                                                                                                                                          aaTCTACCAGTACTACAAAGAGGACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGc             >  2:1379317/1‑150 (MQ=255)
                                                                                                                                           aTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgcc            <  1:1517037/150‑2 (MQ=255)
                                                                                                                                           aTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCAGCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgcc            <  2:2000498/150‑2 (MQ=255)
                                                                                                                                           aTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTGGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgcc            >  2:2413026/1‑149 (MQ=255)
                                                                                                                                           aTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGc             >  2:1234088/1‑149 (MQ=255)
                                                                                                                                           aTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGc             >  2:488433/1‑149 (MQ=255)
                                                                                                                                            tCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTa           >  2:2076442/1‑150 (MQ=255)
                                                                                                                                                 cAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGaa      >  2:363757/1‑150 (MQ=255)
                                                                                                                                                  aGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAAc     >  1:241038/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTG‑T‑ATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTA  >  CP009273/8725‑9021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑