Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,177,475 C→T S733F (TCT→TTT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,177,4750CT100.0% 445.8 / NA 119S733F (TCT→TTT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (59/60);  total (59/60)

Only 100 of 119 total aligned reads displayed.

TATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAG  >  CP009273/4177329‑4177621
                                                                                                                                                  |                                                                                                                                                  
tatCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGc                                                                                                                                                 >  1:735134/1‑150 (MQ=255)
tatCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGc                                                                                                                                                 <  1:2117609/150‑1 (MQ=255)
  tCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCg                                                                                                                                                <  1:1681132/149‑1 (MQ=255)
   cTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCgg                                                                                                                                               >  2:720277/1‑149 (MQ=255)
   cTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCgg                                                                                                                                               >  2:1184129/1‑149 (MQ=255)
      ggCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGca                                                                                                                                             >  1:355277/1‑148 (MQ=255)
      ggCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGca                                                                                                                                             <  1:1816395/148‑1 (MQ=255)
      ggCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGca                                                                                                                                             <  1:165934/148‑1 (MQ=255)
            ggcgAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCg                                                                                                                                     >  2:1183038/1‑150 (MQ=255)
            ggcgAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCg                                                                                                                                     >  1:1595262/1‑150 (MQ=255)
              cgAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGACAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGt                                                                                                                                    >  2:54359/1‑149 (MQ=255)
               gAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCa                                                                                                                                  <  2:1518845/150‑1 (MQ=255)
                aaCGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAg                                                                                                                                 >  1:241778/1‑150 (MQ=255)
                 aCGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGc                                                                                                                                >  2:1630131/1‑150 (MQ=255)
                  cGATCGTGTATCCAAAGCGATGATGGATAACCTACAACCTGCAACCGTGATTAACCGTTACGGACAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATCGCCGATTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCt                                                                                                                               >  1:1887101/1‑150 (MQ=255)
                       gtgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTg                                                                                                                          >  2:5011/1‑150 (MQ=255)
                       gtgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTg                                                                                                                          >  2:631971/1‑150 (MQ=255)
                       gtgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTg                                                                                                                          <  2:150280/150‑1 (MQ=255)
                        tgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTgg                                                                                                                         >  2:499779/1‑150 (MQ=255)
                        tgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTgg                                                                                                                         >  1:1494205/1‑150 (MQ=255)
                         gtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTgg                                                                                                                         >  2:18776/1‑149 (MQ=255)
                            tCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTAt                                                                                                                      <  1:416124/149‑1 (MQ=255)
                              cAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCg                                                                                                                   <  1:1602945/150‑1 (MQ=255)
                               aaaGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGt                                                                                                                  >  1:1147030/1‑150 (MQ=255)
                                aaGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTg                                                                                                                 >  1:1640995/1‑150 (MQ=255)
                                   cGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTc                                                                                                              <  2:1410136/150‑1 (MQ=255)
                                       gatgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGAt                                                                                                          >  2:1534551/1‑150 (MQ=255)
                                         tgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg                                                                                                        >  1:2085303/1‑150 (MQ=255)
                                          ggATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGc                                                                                                       >  2:1400183/1‑150 (MQ=255)
                                            aTAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCg                                                                                                      >  1:961782/1‑149 (MQ=255)
                                                ccTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGcc                                                                                                 <  1:762551/150‑1 (MQ=255)
                                                    cAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGAt                                                                                             <  2:1962034/150‑1 (MQ=255)
                                                     aaaCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATg                                                                                            <  2:1476622/150‑1 (MQ=255)
                                                      aaCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATgg                                                                                           <  2:1582414/150‑1 (MQ=255)
                                                       aCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGc                                                                                          <  2:134249/150‑1 (MQ=255)
                                                         tGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTc                                                                                        >  1:1454987/1‑150 (MQ=255)
                                                         tGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTc                                                                                        >  2:1597200/1‑150 (MQ=255)
                                                            aaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcat                                                                                     >  1:323924/1‑150 (MQ=255)
                                                             aCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcat                                                                                     >  1:2170176/1‑149 (MQ=255)
                                                               cGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcatcat                                                                                  <  2:944406/150‑1 (MQ=255)
                                                                 tGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCg                                                                                <  2:462744/150‑1 (MQ=255)
                                                                   aTTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGa                                                                               >  1:2239363/1‑149 (MQ=255)
                                                                   aTTAACAGTGACGATCAGTAAGAGAAGCAGGTTTCCTGCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTACGACACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGGAGCCGGATGGCTCCATCATCGa                                                                               >  2:1420469/1‑149 (MQ=255)
                                                                       aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACg                                                                           <  1:838133/149‑1 (MQ=255)
                                                                        ccGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGcc                                                                         <  1:1517456/150‑1 (MQ=255)
                                                                          gTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCaa                                                                       >  2:1248381/1‑150 (MQ=255)
                                                                            gACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCTGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAAt                                                                      >  2:1198414/1‑149 (MQ=255)
                                                                             aCGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCa                                                                    <  1:2322039/150‑1 (MQ=255)
                                                                             aCGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCa                                                                    <  1:113538/150‑1 (MQ=255)
                                                                             aCGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCa                                                                    <  1:1894534/150‑1 (MQ=255)
                                                                              cGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATg                                                                                                         >  2:1068602/1‑112 (MQ=255)
                                                                               ggTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg                                                                                                        <  1:1068602/112‑1 (MQ=255)
                                                                                 tCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACcgc                                                                <  1:1200096/150‑1 (MQ=255)
                                                                                    ggAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGaa                                                             >  1:88783/1‑150 (MQ=255)
                                                                                     gAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGGCTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAAc                                                            <  2:701601/150‑1 (MQ=255)
                                                                                      aaGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACt                                                           <  2:2079584/150‑1 (MQ=255)
                                                                                      aaGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACt                                                           >  2:903770/1‑150 (MQ=255)
                                                                                       agagAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACtt                                                          <  1:404273/150‑1 (MQ=255)
                                                                                        gagaAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGGTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTc                                                         >  1:232162/1‑150 (MQ=255)
                                                                                            aGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGt                                                      >  1:1293039/1‑149 (MQ=255)
                                                                                            aGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTg                                                     <  2:1194510/150‑1 (MQ=255)
                                                                                            aGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACAGCGAACTTc                                                         >  2:1994549/1‑146 (MQ=255)
                                                                                             gCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTcc                                                        <  1:1994549/146‑1 (MQ=255)
                                                                                               aGGTTTCCTTCAACATCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGCCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAg                                                  >  1:558921/1‑150 (MQ=255)
                                                                                                ggTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAgg                                                 <  1:1754204/150‑1 (MQ=255)
                                                                                                ggTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAgg                                                 <  2:1640995/150‑1 (MQ=255)
                                                                                                 gTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGt                                                <  2:961782/150‑1 (MQ=255)
                                                                                                    tCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCt                                              >  1:1962785/1‑149 (MQ=255)
                                                                                                     ccTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTg                                             >  2:1533087/1‑149 (MQ=255)
                                                                                                     ccTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTg                                             <  1:2286584/149‑1 (MQ=255)
                                                                                                     ccTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTg                                             <  2:900287/149‑1 (MQ=255)
                                                                                                     ccTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTg                                             >  2:2024396/1‑149 (MQ=255)
                                                                                                      cTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGaa                                           <  1:1311228/150‑1 (MQ=255)
                                                                                                      cTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGaa                                           <  1:1254686/150‑1 (MQ=255)
                                                                                                         cAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAc                                          <  1:18776/148‑1 (MQ=255)
                                                                                                           aCAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTAc                                      <  2:2151551/150‑1 (MQ=255)
                                                                                                            cAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACt                                     <  2:2377758/150‑1 (MQ=255)
                                                                                                              gCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGTTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTc                                    >  2:499405/1‑149 (MQ=255)
                                                                                                                   tACATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAc                              >  2:469187/1‑150 (MQ=255)
                                                                                                                     cATGATGGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTa                               <  2:735134/147‑1 (MQ=255)
                                                                                                                        gatgGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAt                         >  1:67269/1‑150 (MQ=255)
                                                                                                                         atgGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAt                         <  1:556167/149‑1 (MQ=255)
                                                                                                                         atgGCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAt                         <  2:1394710/149‑1 (MQ=255)
                                                                                                                            gCCGACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTcc                     <  1:1534551/150‑1 (MQ=255)
                                                                                                                               gACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccacc                  >  1:933739/1‑150 (MQ=255)
                                                                                                                               gACTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccacc                  >  2:522090/1‑150 (MQ=255)
                                                                                                                                 cTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccaccca                <  2:241778/150‑1 (MQ=255)
                                                                                                                                 cTCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccaccca                <  2:706459/150‑1 (MQ=255)
                                                                                                                                  tCCGGTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccacccac               >  1:1211320/1‑150 (MQ=255)
                                                                                                                                     ggTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGt            >  2:1530222/1‑150 (MQ=255)
                                                                                                                                     ggTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGt            <  1:1400183/150‑1 (MQ=255)
                                                                                                                                     ggTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGt            <  1:611851/150‑1 (MQ=255)
                                                                                                                                      gTGCGCGTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTg           <  1:631971/150‑1 (MQ=255)
                                                                                                                                         cgcgTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTc        <  1:499779/150‑1 (MQ=255)
                                                                                                                                         cgcgTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTc        <  2:799643/150‑1 (MQ=255)
                                                                                                                                         cgcgTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTc        >  2:1782434/1‑150 (MQ=255)
                                                                                                                                          gcgTGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCg       <  1:720277/150‑1 (MQ=255)
                                                                                                                                             tGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTa     >  1:1641379/1‑149 (MQ=255)
                                                                                                                                             tGGTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTa     >  2:360148/1‑149 (MQ=255)
                                                                                                                                               gTTTTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAg  >  1:1024666/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAG  >  CP009273/4177329‑4177621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑