Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BA000036	1,962,935	T→C	V22V (GTA→GTG)	Cgl1848 ←	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BA000036	1,962,935	0	T	C	100.0%	166.9 / NA	58	V22V (GTA→GTG)	Cgl1848	Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

GTGATACTCAATGACTACTTCTGGATGTGCTGATTCGAAATCCTCAAGTTGTTCCTTAACAGTCGGAGCATTCCTATTGGGAACATAGTCAACACCATCATCAGCAAACTGCTCATCACTGATTTCTTTTACAAGCTCAAATTGATCTTCCACTTGTGCTTGAGCTACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATAGTTCAATGCAGCA  >  BA000036/1962770‑1963229                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       ctGATACTCAAAGACTACTTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTa                                                                                                                                                                    <  2:284593/297‑1 (MQ=11)  tGATACTCAACGACTACTTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATAGCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTaaaa                                                                                                                                                                 <  1:315446/300‑1 (MQ=11)            cgACTACTTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATggg                                                                                                                                                       >  1:117590/2‑300 (MQ=11)             gACTACTTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATgg                                                                                                                                                        >  1:375899/1‑298 (MQ=11)             gACTACTTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGga                                                                                                                                                      <  2:472263/300‑1 (MQ=11)               ctactTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGga                                                                                                                                                      >  1:415818/1‑298 (MQ=11)                tactTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAA‑CCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATACGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGgagaga                                                                                                                                                  <  1:159538/300‑1 (MQ=14)                  ctTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGATATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCCAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAACGTTGGATGGgagaga                                                                                                                                                  >  1:544070/1‑299 (MQ=11)                  ctTCTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGgagag                                                                                                                                                   <  2:20824/298‑1 (MQ=11)                     cTGGATGTGCTGTTTCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGaaaa                                                                                                                                               <  1:487013/299‑1 (MQ=14)                          tgtgATGTTGCGTAATCCTCAAGTTGTTCCTCGACTGTTGGAGCAGTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATg                                                                                                                                         <  2:98045/300‑1 (MQ=14)                              gtgtttCGTAATCCTGAAGTTGGTCCTCAACTGTGGGAGCATTCCTATTGGGAACATAATCAACACCATCAGCAGCAAACTACTCATCACTGACTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCGTCGTACCAACCGCATATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGctct                                                                                                                                     <  2:392654/296‑1 (MQ=14)                                 tttctgcagccTCAAGGTTTTCCTCAACGTGTGGAGCATTCCTATTGGGAACATAAGCAACACCGGCATCAGCAAACTACTCATCACTGAGTTCGTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCtt                                                                                                                                    <  2:91890/289‑1 (MQ=17)                                 tttCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTa                                                                                                                                  <  1:250709/299‑1 (MQ=14)                                  ttCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAACCCTTTACCTGTCGGAAAATATATACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTAc                                                                                                                                 >  1:19782/1‑300 (MQ=14)                                  ttCGTAATCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTAc                                                                                                                                 >  1:19789/1‑300 (MQ=14)                                    cgtaaTCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATATTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACAGAGGATAGGTAAAGAATTAGTTAAAAGTGGGATGGGAGAGAAAATAAATGCTCTTTAccc                                                                                                                               >  1:144796/4‑300 (MQ=14)                                     gtaaTCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTAccc                                                                                                                               <  1:47117/297‑1 (MQ=255)                                        aTCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGaa                                                                                                                            >  1:442173/1‑299 (MQ=255)                                         tCCTCAAGTTGTTCCTCAACTGTTGGAGCATTCCTAGTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGaaaa                                                                                                                          <  2:280530/300‑1 (MQ=17)                                               aGTTGTTCCTCAACTGTTGGAGCATTCCTCTTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAgggg                                                                                                                    >  1:361248/1‑300 (MQ=17)                                                  tgttCCTCAACTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACCAACTGAGGCTAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGCAAAGAGGGGTg                                                                                                                  >  2:39897/1‑299 (MQ=17)                                                      ccTCAACTGTTGGAGCATTCCTATTGGGAACATCCTCAACACCGTCATCAGCAAACTACTCCTCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCCCCTTTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGaaaa                                                                                                              <  2:14536/299‑1 (MQ=17)                                                       ctcaaCTGTTGGAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAACGa                                                                                                            >  2:223482/4‑300 (MQ=17)                                                       ctcaaCCGTTGGATCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACCGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGa                                                                                                            >  2:223470/4‑300 (MQ=18)                                                             tgttggAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGCAAAGACTgcgt                                                                                                      >  1:408657/5‑300 (MQ=255)                                                             tgttggAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTgtgt                                                                                                      >  1:315868/5‑300 (MQ=255)                                                                tggAGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCCTCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGggttg                                                                                                    >  2:509861/2‑299 (MQ=255)                                                                   aGCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACt                                                                                                >  1:514970/1‑300 (MQ=255)                                                                    gCATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACtt                                                                                               <  1:541044/300‑1 (MQ=255)                                                                     cATTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCGGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACttt                                                                                              <  2:442173/300‑1 (MQ=255)                                                                      aTTCCTATTGGGAACATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTa                                                                                             <  1:420179/300‑1 (MQ=255)                                                                                    cATAATCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGct                                                                               <  2:280603/300‑1 (MQ=255)                                                                                         tCAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGCTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCg                                                                          <  1:399837/300‑1 (MQ=255)                                                                                          cAACACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGCTGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGCGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCgg                                                                         >  2:415320/1‑300 (MQ=255)                                                                                           aaCACCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCgg                                                                         >  1:115619/1‑299 (MQ=255)                                                                                              acCGTCATCAGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGa                                                                     >  1:412086/1‑300 (MQ=255)                                                                                                      caGCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTATCCGGTAGACGGATCGa                                                             >  2:465151/1‑300 (MQ=255)                                                                                                        gCAAACTACTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGAAGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGaca                                                           <  2:117590/300‑1 (MQ=255)                                                                                                              tacTCATCACTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGCCACCCTa                                                      >  2:543092/3‑299 (MQ=255)                                                                                                                cTCATCACTGACTGCTTCGACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATac                                                     <  2:488311/298‑1 (MQ=255)                                                                                                                       cTGATTTCTTCTACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCtt                                             >  1:118840/1‑299 (MQ=255)                                                                                                                               ttctACAAGCTCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGCCAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCCAACCTTTACCTGTCGGAAAATATGTACAAACTGCGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATATGCTCTCAGGTAGACGGATCGACACCATACACGGTCTTAAACATTTc                                    >  1:58779/4‑300 (MQ=255)                                                                                                                                tctACAAGCTCAAAGTGATCTTCCACCTGTGCTTGAGCCAACGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCCACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTc                                    <  2:267095/297‑1 (MQ=255)                                                                                                                                 ctacactcTCAAATGGAGCGTCCACCTGTGCTTGAGCCACCGGAGAAGCAACCCCGATGGCCAGCAACATGACATCAGAAACACCCACTAGTCTGCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTc                                    <  2:267077/291‑1 (MQ=255)                                                                                                                                         tCAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACa                                                                                                                                           >  2:382146/1‑187 (MQ=255)                                                                                                                                          cAAATTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACAt                                                                                                                                          <  1:382146/187‑1 (MQ=255)                                                                                                                                           aaaTTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGccacc                          <  2:155757/298‑1 (MQ=255)                                                                                                                                           aaaTTGATCTTCCACCTGTCCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCcaccac                        <  2:266926/300‑1 (MQ=255)                                                                                                                                            aaTTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACt                       >  1:92254/1‑300 (MQ=255)                                                                                                                                             aTTGATCTTCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTg                      >  1:499941/1‑300 (MQ=255)                                                                                                                                             aTTGATCTGCCACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAACAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTg                      >  1:499934/1‑300 (MQ=255)                                                                                                                                                      ccacccgtgCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATa               <  2:284516/292‑1 (MQ=255)                                                                                                                                                      ccACCTGTGCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATa               <  1:246459/298‑1 (MQ=255)                                                                                                                                                         ccttttcTTGGGCCACCGGTGCTGCAACCCCGCTTGCCAGCAACCTTACAGCAGAAACACCCACTACTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAACAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATAGTTCa          <  2:331072/294‑1 (MQ=255)                                                                                                                                                         cctgtgCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATAGTTCa          <  2:461600/298‑1 (MQ=255)                                                                                                                                                         cctgtgCTTGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACACGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATAGTTCa          <  2:136953/298‑1 (MQ=255)                                                                                                                                                                 tGAGCCACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTCAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATAGTTCAATgcagca  >  2:374325/1‑300 (MQ=255)                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       GTGATACTCAATGACTACTTCTGGATGTGCTGATTCGAAATCCTCAAGTTGTTCCTTAACAGTCGGAGCATTCCTATTGGGAACATAGTCAACACCATCATCAGCAAACTGCTCATCACTGATTTCTTTTACAAGCTCAAATTGATCTTCCACTTGTGCTTGAGCTACCGGAGATGCAACCCCGATGGCCAGCAACATGACAGCAGAAACACCCACTAGTCTTCGTACCAACCGCTTATATCAAACCTTTACCTGTCGGAAAATATGTACAAACTGAGGATAGGTAAAGAATTAGTTAAAAGTTGGATGGGAGAGAAAATACATGCTCTTTACCCGAAAAGAGGGGTGAAAAGACTGTGTTGTACTTTAAGTGGGAATAGGCTCTCCGGTAGACGGATCGACACCATACACGGTCTTAAACATTTCTGGTGCCACCACTGCCTGATAGTTCAATGCAGCA  >  BA000036/1962770‑1963229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑