BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BA000036	2,160,737	C→T	P23L (CCT→CTT)	Cgl2047 →	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BA000036	2,160,737	0	C	T	96.6%	313.0 / NA	90	P23L (CCT→CTT)	Cgl2047	Hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (45/41); minor base G (1/2); total (46/44)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTGGAAAGGACTGCCGCCTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACC‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGATTTTTGCATCTCACCATCCACCGCGGTGCGGATC  >  BA000036/2160440‑2161033

ttGGAAAGGACTGCCGCCTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACG‑Tg                                                                                                                                                                                                                                                                                                        <  1:476441/300‑1 (MQ=255)

  ggAAAGGACTGCCGCCTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGcc                                                                                                                                                                                                                                                                                                      >  1:112612/1‑300 (MQ=255)

     aaGGACTGCCGCCTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAATTAGGAACACTCACACT‑TGCCCCa                                                                                                                                                                                                                                                                                                   >  1:240127/1‑300 (MQ=255)

       ggACTGCCGCCTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGCGGAATCGTATCCACAAGGGTATGATGACCGTTATTCCTGGGGCCGAAAAGTAGGAAAACTCGCACT‑TTACCCAcg                                                                                                                                                                                                                                                                                                 >  2:367281/1‑300 (MQ=255)

         aCTGCCGCCTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATCATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCAcgcg                                                                                                                                                                                                                                                                                               >  1:293907/1‑300 (MQ=255)

              cgccTGGTTGATCTGTGCAGCATCGTCTGCACTGATCACACCATGGCGGGCTTGAGCAGTTGGGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGtt                                                                                                                                                                                                                                                                                          <  2:118549/300‑1 (MQ=255)

                 cTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCGTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATGTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTGGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATGGTAACCACAAAGGTATGAGGATCGTTAGTCATGGAGCCGAAAAGGAGGAAAACCAAAAAG‑TGCCCAACgcggcggtgt                                                                                                                                                                                                                                                                                        >  2:345540/1‑295 (MQ=255)

                  tGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGtttt                                                                                                                                                                                                                                                                                        >  1:446109/1‑298 (MQ=255)

                              gcagcaTCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCCCGCGGCGTTTTAAGCGCTCAGAttt                                                                                                                                                                                                                                                                          >  1:493726/1‑300 (MQ=255)

                                 gcaTCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATGGTGGCATCTGAAGAAAAGATTTCTTGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTgag                                                                                                                                                                                                                                                                       <  2:360209/300‑1 (MQ=255)

                                      gTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAAc                                                                                                                                                                                                                                                                   <  1:286618/299‑1 (MQ=255)

                                            aGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCAccc                                                                                                                                                                                                                                                             >  1:47926/1‑299 (MQ=255)

                                             gTGATCACCCCCTGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGCTTTCTCGTTGAGTGTCATTTCCTGCGTACTGCCACAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAACGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTACGCGCTCAGCTTTGAACAACGCACCCgc                                                                                                                                                                                                                                                           >  2:138363/1‑299 (MQ=255)

                                                      ccATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCTCTCAGATTTGATAAACGCACCCGAGGGTCACTc                                                                                                                                                                                                                                                  >  1:428032/1‑300 (MQ=255)

                                                      ccATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATc                                                                                                                                                                                                                                                  >  1:428026/1‑300 (MQ=255)

                                                           gCGGGCTTGAGCAGTTGGGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTc                                                                                                                                                                                                                                             <  1:311754/300‑1 (MQ=255)

                                                                   gAGCGGTTGGGAGGACTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTa                                                                                                                                                                                                                                       <  2:25310/298‑1 (MQ=255)

                                                                       aGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTAcctcct                                                                                                                                                                                                                                 <  1:537128/300‑1 (MQ=255)

                                                                        gTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAACAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTGTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTAcctcct                                                                                                                                                                                                                                 >  2:93523/1‑299 (MQ=255)

                                                                            agagtgccttacccGCAGCCAACCAGGTGTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCGGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGTGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAAc                                                                                                                                                                                                                            <  2:78192/288‑1 (MQ=255)

                                                                            agagTACTCTCTCCGCAACCAAGCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGACTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTAAGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGAAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAAAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTaaa                                                                                                                                                                                                                             <  1:325876/299‑1 (MQ=255)

                                                                             gagTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACg                                                                                                                                                                                                                           <  2:448895/300‑1 (MQ=255)

                                                                                tgcgttctcCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTGTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTc                                                                                                                                                                                                                        <  1:70784/295‑1 (MQ=255)

                                                                                tGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAACGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAACGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTACACGGt                                                                                                                                                                                                                         >  2:430787/1‑299 (MQ=255)

                                                                                        ccGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGTGCTCAGATTTGAGAACCGCACCCGAGGGTCAACCTGGTCGAGCTACCTCCTTATACGGTCATATaaa                                                                                                                                                                                                                 >  2:148642/1‑299 (MQ=255)

                                                                                                 aCCAGCTTGCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAAcatcatcat                                                                                                                                                                                                       <  1:349147/300‑1 (MQ=255)

                                                                                                    aGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTc                                                                                                                                                                                                    >  1:278525/1‑300 (MQ=255)

                                                                                                       tttCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGCAAAGTAGGAAAACTCACACT‑TGCCCCCCGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTACACGGTCATATAGACCATCCTCATTTCCGc                                                                                                                                                                                                 >  2:499573/1‑300 (MQ=255)

                                                                                                       tttCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGc                                                                                                                                                                                                 >  2:372815/1‑300 (MQ=255)

                                                                                                                     tCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGCGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGAt                                                                                                                                                                                   >  2:548063/1‑300 (MQ=255)

                                                                                                                      cTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCACAGTTAGGAGAATGTGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCCGCGTTTTAAGCGCTCAGATTTGAGAAACGCCCCCGAGGCTCAATCTGGTCGAGTTACCTCCTTAACCGGTCATATATAACATCATCATTTCCGCCTGTCATGGGCGAtt                                                                                                                                                                                  >  2:264598/1‑300 (MQ=255)

                                                                                                                      cTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATCAAACATCATCATTTCCGCATGTAATGGGCGAtt                                                                                                                                                                                  >  2:264599/1‑300 (MQ=255)

                                                                                                                         aagaaAAGATTTCTGGTTGGGTTTCATGTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGAt                                                                                                                                                                                   <  2:453910/296‑1 (MQ=255)

                                                                                                                         aagaaAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGAtttt                                                                                                                                                                                <  1:326906/299‑1 (MQ=255)

                                                                                                                            aaaaGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGaca                                                                                                                                                                            >  1:33205/1‑300 (MQ=255)

                                                                                                                              aaGATTTCTAGTTGAGTGTCTTTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACTTTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTTGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATGTTGACAcc                                                                                                                                                                          <  1:553743/300‑1 (MQ=255)

                                                                                                                                  tttCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTAACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCAAATAAAACATCATCATTTCCGCATGTAATTGACGATTTGGACACTtgt                                                                                                                                                                       >  2:293243/1‑299 (MQ=255)

                                                                                                                                      tAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCtgt                                                                                                                                                                       >  1:294965/1‑295 (MQ=255)

                                                                                                                                       aGTTGGGTGTCATGTCTGGCGTGCTGCCAAAGTTAGAGGAATGGGTGTTTGTCAGTGGGATACGTTGTGCGCGCTCCTAGCACCTATGGGCTGGTATGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGGGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGGGTTACCTCCTTAAACGGTCATATAAAACATCATCATATCCGCATGTAATGGGCGATTTTGACACCTGTGAGATc                                                                                                                                                                 <  2:120025/300‑1 (MQ=255)

                                                                                                                                                   tttCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGaa                                                                                                                                                     >  2:382144/1‑300 (MQ=255)

                                                                                                                                                     tCCTGCGTACTGCCAAAGTTAGGAGAATGTGGCGTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATGTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAAt                                                                                                                                                    <  2:113531/299‑1 (MQ=255)

                                                                                                                                                        tGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGa                                                                                                                                                >  1:86999/1‑300 (MQ=255)

                                                                                                                                                             aCTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGTATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGACGGTCAATCTTGTCGAGTTACCTCCTTAACCGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGTTTTTGACACCTGTTAGATCCCTGATCAAGAATCAGACCAGc                                                                                                                                           >  2:347474/1‑300 (MQ=255)

                                                                                                                                                               tGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGc                                                                                                                                           >  1:60125/1‑298 (MQ=255)

                                                                                                                                                                   aaaGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCa                                                                                                                                      >  1:160577/1‑299 (MQ=255)

                                                                                                                                                                    aaGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTGTTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATa                                                                                                                                    <  2:486695/300‑1 (MQ=255)

                                                                                                                                                                       ttAGGAGAATGGGGCTTTGTCAGTGGGATACATTGGGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCt                                                                                                                                 <  1:294365/300‑1 (MQ=255)

                                                                                                                                                                          ggAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACCAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCAGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTg                                                                                                                                >  1:113653/1‑298 (MQ=255)

                                                                                                                                                                                tGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTCTGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTCTTCCTGGAGCCGAACAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTACACGGTCATATTAAACATCATCATTTCCGCATGTAATCGGCGATTTTGCCCCCTGTGAGATCCCTGATCAAGCATCCGCCCAGCTAGTATACCTGAAGctct                                                                                                                         >  2:277141/1‑299 (MQ=255)

                                                                                                                                                                                     cTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATc                                                                                                                    >  1:428268/1‑299 (MQ=255)

                                                                                                                                                                                     cTTTGTCAGGGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATcg                                                                                                                   <  1:486840/300‑1 (MQ=255)

                                                                                                                                                                                            agggggATACATTGTGCGCGCTCCGAGCACCTATGGGCTTGTGTGGAATCGTAGCCACAAAGGTAGGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATcgaacgaag                                                                                                            <  2:115528/297‑2 (MQ=255)

                                                                                                                                                                                                ggATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATCATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAAttttt                                                                                                        <  2:293907/300‑1 (MQ=255)

                                                                                                                                                                                                 gATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTg                                                                                                       <  1:253091/300‑1 (MQ=255)

                                                                                                                                                                                                  aTACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TTCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTAACTCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATATCCGCATGTCATGGGCGATTTTGACACATGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTTAACAGAACGACTTTTTGc                                                                                                      >  1:58481/1‑300 (MQ=255)

                                                                                                                                                                                                  aTACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAAAATCCAACACGCTAGCATACCTGAAGCTCTAAATAGAACGAATTTTTg                                                                                                       >  1:58472/1‑299 (MQ=255)

                                                                                                                                                                                                    aCATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTCTACGCGCTCAGATTTGAGAAACGCACCCGAGGGCCAATCTGGTCGAGTTACCTCCTTAAACGGCCCTATAAAACATACTCATTTCCGACTGTAATGGGCGATTTTGCCACCTGTGAGATCCCTGATCAAGAAACAGACCAGCTGGCATACCAGAACGGCTAACTCGAACGAAtttgttact                                                                                                    >  2:371522/1‑294 (MQ=255)

                                                                                                                                                                                                      aTTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATg                                                                                                   >  1:336312/1‑299 (MQ=255)

                                                                                                                                                                                                         gtgCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGaa                                                                                                <  1:34483/299‑1 (MQ=255)

                                                                                                                                                                                                           tggcgcTCCTGGCACCTATGGGCTTGGGTGGAAACGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGACGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCGTAAACGGTCATATAAAACATCATCATTTCCGGATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAAcc                                                                                              <  1:471778/297‑1 (MQ=255)

                                                                                                                                                                                                           gcgcgcTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTACTCCTGTAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAActt                                                                                             >  1:482970/1‑298 (MQ=255)

                                                                                                                                                                                                            cgcgaTCCTAGCACCTATGAGCTTGTGAGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGGAAACTCACACT‑TGCCCCACGCGGCGTTTGAAGCGCTCAGATTTGAGAAACGCACCCGAGGGACAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCGGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTc                                                                                            <  2:335419/300‑1 (MQ=255)

                                                                                                                                                                                                             gcgcTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCg                                                                                           <  2:80966/300‑1 (MQ=255)

                                                                                                                                                                                                             gcgcTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCg                                                                                           >  1:415179/1‑300 (MQ=255)

                                                                                                                                                                                                             gcgcTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATACCTGGAGCCGAAAAGTAGGAGAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCg                                                                                           <  1:367281/300‑1 (MQ=255)

                                                                                                                                                                                                                 tCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCtt                                                                                       >  2:474919/1‑300 (MQ=255)

                                                                                                                                                                                                                     aGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCGCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCATAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGCACGAAATATTGCATGGAACATCGGCTTTc                                                                                     >  1:27845/1‑298 (MQ=255)

                                                                                                                                                                                                                      gtactgaTGGGCTTATGTGAAATCGTATACAAATAGGTATGATGATCGTTAATCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGATCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAGAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTg                                                                                  <  1:148642/294‑1 (MQ=255)

                                                                                                                                                                                                                       cACCTATGGGCGTGTGTGGAATCGTGTCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACACGGCGTTTTAATCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTGTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGc                                                                                 <  1:469107/300‑1 (MQ=255)

                                                                                                                                                                                                                          ctatgggctgtcgatgAATCGTCTCCATAAAGGGTTGATGACCGTTATTCCTGGAGCCGTAAAGTTGGAAAACTCACCCT‑TGCCCCACGCGGCGTTGTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATGTTGACACCTGTGAGATCCCTGATCAATAATCCGACCCGCTAGCATACCTGAAGCGCTAAATCGAACGAATTTTTGCATGGAACCTCGGATTTCGTGCaaa                                                                              <  1:360108/285‑1 (MQ=255)

                                                                                                                                                                                                                               ggCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTc                                                                         <  2:278525/300‑1 (MQ=255)

                                                                                                                                                                                                                                    gtgagGAATCGTATCCAAAAAGGGATGATGATCGTTATGCCGTGATCCGAAAAGTAGGAGAACTCACACG‑TGCCGCACGCGGCGTTTTAAGCGCTAAGATATGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAGACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCGAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTGTGCATGGAACCTCGGCTATCGTGCAAATTTTCGACt                                                                     <  2:204205/299‑1 (MQ=255)

                                                                                                                                                                                                                                         gAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTGCGACTGAGTTc                                                               <  2:123166/300‑1 (MQ=255)

                                                                                                                                                                                                                                         gAATCGTATCCACAAAGGTATGATGATCGTTATTCCGGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTc                                                               <  2:47926/300‑1 (MQ=255)

                                                                                                                                                                                                                                          aaTCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGAcc                                                                                                                                              >  2:322156/1‑220 (MQ=255)

                                                                                                                                                                                                                                           aTCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCa                                                                                                                                             <  1:322156/220‑1 (MQ=255)

                                                                                                                                                                                                                                                aTCCAAAAAGGTATGATGATCGTTAATCCGGAAGCCAAAAAGTAGGAAAACACGCACT‑TGCCCCACGCGACGTTGTAGGCGCGCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTAACCTCCTTAAGCGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATAAAGAATCCGACCAGCTAGCAGACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGATTTCGTGCAAATTTGAGACTGATTTCGGGTccc                                                        <  2:342030/300‑1 (MQ=255)

                                                                                                                                                                                                                                                     caAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGCTTTCGGGTCCccacca                                                   >  1:72341/1‑300 (MQ=255)

                                                                                                                                                                                                                                                     caAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGAGTTCGGGTCCccacca                                                   <  2:294965/300‑1 (MQ=255)

                                                                                                                                                                                                                                                         ggTATGATGATCGTTATGCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCa                                               <  1:408420/300‑1 (MQ=255)

                                                                                                                                                                                                                                                              gctactCGTTATTCCTGGCGCCGAAAAGCAGTAAAACTCAAACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATGTGAGAGACGCACCCAAGGGTCAATCCGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCAGCATTTCCGCATGTAATGGGCGATTTGGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAAtcg                                          <  2:430357/295‑1 (MQ=255)

                                                                                                                                                                                                                                                               atgatCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGCAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAACTTTTCGACTGGTTTCGGGTCCCCCCCACCCAAAtcgg                                         >  2:308345/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                 tgtCGTTATTCCTGGAGCAGAAAAGTAGG‑AAACCCAAACCTTGGCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAGACGCACCCGAGGGTCAATCTGGTGGGGTTACCTCCTTAAACGGTCATATAAGACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAGTCCGACCAGCTAGCAGACCGGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAAGTTTCGACTGATTTCGGGTACCCACAACCCAAAtcggtc                                       <  2:555203/298‑1 (MQ=255)

                                                                                                                                                                                                                                                                      ttATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAATTTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGAtt                                  >  1:113424/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                       tATTCCTGGAGCCGAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGTCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTTCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTCGCTTACCTGAAGCTCTAAATCGAACGAATTTTTGCATGTAACCCCGGCTTTCGTGCAAATCTCCGACCGAGTACGGGTCCCCACCACTCAAATCGGTCGGAttt                                 >  2:497350/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                    gAAAATTAGGAAAACTCCCACT‑TGCCCCACGCGGCGTTTGAAGCGCTCAGATTTGAGAAACGCACCAGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGAGTTTTGCATCTCACCa                    <  2:428032/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                    gAAAAGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGAGTTTTGCATCTCACCa                    <  2:428026/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                       aaGTAGGAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGATTTTTGCATCTCACCATcc                 <  1:372815/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                            ggAAAACTCACACT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAACTCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGATTTTTGCATCTCACCATCCACcgc            >  1:379407/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                                      cacT‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGATTTTTGCATCTCACCATCCACCGCGGTGCggtta  >  1:135106/1‑297 (MQ=255)

TTGGAAAGGACTGCCGCCTGGTTGATCTGTGCAGCATCGTCTTCAGTGATCACACCATGGCGGGCTTGAGCAGTTGAGAGTGCTCTCTCCGCAGCCAACCAGCTTTCGATTGTGGCATCTGAAGAAAAGATTTCTAGTTGAGTGTCATTTCCTGCGTACTGCCAAAGTTAGGAGAATGGGGCTTTGTCAGTGGGATACATTGTGCGCGCTCCTAGCACCTATGGGCTTGTGTGGAATCGTATCCACAAAGGTATGATGATCGTTATTCCTGGAGCCGAAAAGTAGGAAAACTCACACC‑TGCCCCACGCGGCGTTTTAAGCGCTCAGATTTGAGAAACGCACCCGAGGGTCAATCTGGTCGAGTTACCTCCTTAAACGGTCATATAAAACATCATCATTTCCGCATGTAATGGGCGATTTTGACACCTGTGAGATCCCTGATCAAGAATCCGACCAGCTAGCATACCTGAAGCTCTAAATCGAACGAATTTTTGCATGGAACCTCGGCTTTCGTGCAAATTTTCGACTGATTTCGGGTCCCCACCACCCAAATCGGTCGGATTTTTGCATCTCACCATCCACCGCGGTGCGGATC  >  BA000036/2160440‑2161033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`