Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BA000036414,9241.A26.1% 40.3 / 22.3 23coding (211/816 nt)Cgl0391ABC‑type transporter, ATPase component
Reads supporting (aligned to +/- strand):  ref base . (8/9);  new base A (0/6);  total (8/15)
Fisher's exact test for biased strand distribution p-value = 5.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCTGCCGATCGGCATTTTCACAGCGCTGGTCGGCGGCCCAACATTCTTCATAATGTTGCGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGATGGATA  >  BA000036/414626‑415221
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                         
ccTGCCGATCGGCATTTTCACAGCGCGGGTCGGCGGCCCAACATTCTTCATAATGTTGCGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACagca‑g‑c                                                                                                                                                                                                                                                                                                          <  2:550071/300‑1 (MQ=255)
                     aGCGCGGGTCGGCGGCCTAACATTCTTCATAATGTGGCGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGt                                                                                                                                                                                                                                                                                     <  2:178222/300‑1 (MQ=255)
                                           ttcttcATTATGTTACGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGTTTTTGATTTC‑AAGCCGAA‑GACGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGAt                                                                                                                                                                                                                                                               <  1:129672/300‑1 (MQ=255)
                                                cATAATGTTGCGCCGAATGATGAAAAAGGGCGTGCATGAAATGGCTATTGTTTCCCGCGACAACGTCACCGTATCAATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGTTTTTTTTTTC‑AAGCAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGAGGt                                                                                                                                                                                                                                                             <  1:374511/297‑1 (MQ=255)
                                                     tGTTGCGCCGAAGGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTAGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTGGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTAGTTTCC‑AAGAAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTgg                                                                                                                                                                                                                                                     <  1:244645/300‑1 (MQ=255)
                                                             ccaaTTATGAACAAGGGCGTCACCTACAGGGCCATGGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCTGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTTTTTTCCGCGCCGACG‑A‑CGGCATCCGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTttcc                                                                                                                                                                                                                                             <  1:166976/298‑1 (MQ=255)
                                                                                          ggCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGGTCGAACTCATCGGCCCACACGGTGCCGTAAAATCCACTCTGCTGAGTGTCCTTTCAGGCTATCGGCTTCCCGATTCCGGCGAAGGCAACGTCGGTGGCTTAGATCCCGCCACAGCA‑T‑CGGCAGCCGATATGGCCAGGGTGCGAGCAGGCATGCTTCGAGATGTCAGCGTGGCATTTTCCGTCCTTGTGTCGGACGTCGGAGGAATcgg                                                                                                                                                                                                                >  2:306787/1‑300 (MQ=255)
                                                                                              aTTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAACGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCCGAGTGTCCGTTCAGGCGATCGGCTTCCCGAGTCAGACGACGTCAACGTCGGTGGCTTAGATCCCGCACCAGAA‑G‑CGGCATCCGATCTGGCCAGGCGCCGAGCAGTCCTGCTCCAAACTGTCAGCCAGGCCTTTTCCTTCCTCCTTTGGCACGTCGTCGCAATAGGCCgg                                                                                                                                                                                                            >  2:169706/1‑300 (MQ=255)
                                                                                              aTTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCACACGGTGCCCGAAAATCCACTCTGCTCAGTGTCCTTTCAGGCGATCGGCCTCCCGATCCAGGCGACGTCAACGCCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGCTATGGCCAGGTTGCGCGCACTCCTCATTCCAGCCTCCAGCACGCCACTTTCCTTCCTCCTGTCGGgcggcgacgacataggccac                                                                                                                                                                                                            >  2:169700/1‑286 (MQ=255)
                                                                                                                gTCAACGGATCCATTGACGGAAAAAAGCTCCTCGACGCCGTCTCCCTCACGGCCTACCCCGGGGACGTGGTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGCGCGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGCCGGTGGCTTAGATCCCGCACCAGCA‑G‑CGCCATCCGATATGGCCACGGTGCGAGCGGTCATTCTTCCAGATGTCAGCGTGGCCTTATCCTTCCTCGCTGGTGACTTCATAGAAATGTGCACGCGGCCGTCGCACAAGGCg                                                                                                                                                                                          >  2:247700/1‑300 (MQ=255)
                                                                                                                            aTTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCAGATGCAGGCGAAGTCAACGTCGGTGGCTGAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGaa                                                                                                                                                                              <  2:468131/300‑1 (MQ=255)
                                                                                                                             ttGAAGGAAAAAAGCTTCTCGACGCCGTCGCACTCAAGGCCTACCCCGGGGGAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACGCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGGGGTTTAGATCTCGAAACGGCG‑G‑CGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAag                                                                                                                                                                             <  1:484496/300‑1 (MQ=255)
                                                                                                                                                 taCGCCGTCGCCCTCAAGGCCTACCCCGGGGAAGTGTTGTGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGTCGAAGTCAACGTCGGTGGCTTATCTTCCGCACCGGCG‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCg                                                                                                                                                           <  1:452890/297‑1 (MQ=255)
                                                                                                                                                           cccTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCACACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCTAGATGGCAGCGTGGGATTTTCTTACCTCGTGTGGGACGTCGTAGAACTGGGCAGGCGGCCGTGGCTGAAGGCGTCAACTCCCGACGAGGGTCAGGAAATCATAGAACCAGCGCGTg                                                                                                                                               >  2:519836/1‑300 (MQ=255)
                                                                                                                                                                   gCCTACCCCGGGGAAGGGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGTTTTATTTTTC‑AAGCAGAAGG‑CGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCAcc                                                                                                                                       <  1:306787/300‑1 (MQ=255)
                                                                                                                                                                                           cTCATCGGCACAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTAAGGCGATCGGCGTCCCGATTCAGGCGAAGTCGACGTCGGTTGCTTATATTTC‑AAGCAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTAGCGCACCTTGCCGAACgg                                                                                                               <  1:433535/300‑2 (MQ=255)
                                                                                                                                                                                              aTCGGCCCAAACGGGGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTAGATCCCGAGAAGGCA‑G‑CGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGaa                                                                                                            <  1:519836/300‑1 (MQ=255)
                                                                                                                                                                                                              ccGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGAAACGAAG‑G‑CGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCa                                                                                             <  1:518031/299‑1 (MQ=255)
                                                                                                                                                                                                                  aaaaTCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATGTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATGACGAGATCATCGAAGCAGAGCATGCCGCCACCTCGGTATCGCACCTTGCCGAACGGGAAATCACCACAGTGCCAcgcgg                                                                                        >  2:367773/1‑300 (MQ=255)
                                                                                                                                                                                                                         atgctgctgAGTGTCCTTGCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTTGTTTTC‑AAGAAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCgg                                                                                 <  1:30872/297‑1 (MQ=255)
                                                                                                                                                                                                                              gctgAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGCAGCAGCGCTTGCCGCCCCCTCGGTAGCGCCCCTTGCCGAACGTGAAATCACCACACTGTCCGGAGGCGACCGGGCAcg                                                                            >  2:37932/1‑300 (MQ=255)
                                                                                                                                                                                                                                  aGTGTCCTTGCAGGCGATCGTCTTCCCGATTAAGGCGAGTTCAACGTCGGTGTCTTTGTTTCCG‑AGCAAAA‑GACGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGt                                                                         <  1:57309/299‑1 (MQ=255)
                                                                                                                                                                                                                                           tCAGGCGATCGGCCTCCCGATTCAGGCGCAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTCCACGATGTCAGCGTGGCATTTCACTTCCCCGTGTGGGACGTCGTAGAACTGGGCATGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGACACAGCGCTTGCCGCCAACCCGGTATCGCACATCGTCGAACGTGAAAGCACCACAATGTAAGGCAGCGCGCCGGCTcgcgcgccctcggcc                                                               >  2:240438/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                      gCTTAGTTCCCGCAACGGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGaa                     <  1:366089/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                        ca‑g‑cGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCCCCTCGGTATCGCACCTTGCCGACCGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCCCGCCTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGAGGGat   >  1:60813/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                        ca‑g‑cGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGATGGata  >  1:69851/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                         
CCTGCCGATCGGCATTTTCACAGCGCTGGTCGGCGGCCCAACATTCTTCATAATGTTGCGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGATGGATA  >  BA000036/414626‑415221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑