| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BA000036 | 414,931 | 0 | C | A | 40.0% | ‑1.0 / 18.8 | 25 | S73Y (TCC→TAC) | Cgl0391 | ABC‑type transporter, ATPase component |
| Reads supporting (aligned to +/- strand): ref base C (10/5); new base A (0/10); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.65e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGCTGGTCGGCGGCCCAACATTCTTCATAATGTTGCGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGATGGATATCAGCCACC > BA000036/414647‑415230 | aGCGCGGGTCGGCGGCCTAACATTCTTCATAATGTGGCGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGt < 2:178222/300‑1 (MQ=255) ttcttcATTATGTTACGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGTTTTTGATTTC‑AAGCCGAA‑GACGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGAt < 1:129672/300‑1 (MQ=255) cATAATGTTGCGCCGAATGATGAAAAAGGGCGTGCATGAAATGGCTATTGTTTCCCGCGACAACGTCACCGTATCAATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGTTTTTTTTTTC‑AAGCAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGAGGt < 1:374511/297‑1 (MQ=255) tGTTGCGCCGAAGGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTAGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTGGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTAGTTTCC‑AAGAAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTgg < 1:244645/300‑1 (MQ=255) ccaaTTATGAACAAGGGCGTCACCTACAGGGCCATGGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCTGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTTTTTTCCGCGCCGACG‑A‑CGGCATCCGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTttcc < 1:166976/298‑1 (MQ=255) ggCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGGTCGAACTCATCGGCCCACACGGTGCCGTAAAATCCACTCTGCTGAGTGTCCTTTCAGGCTATCGGCTTCCCGATTCCGGCGAAGGCAACGTCGGTGGCTTAGATCCCGCCACAGCA‑T‑CGGCAGCCGATATGGCCAGGGTGCGAGCAGGCATGCTTCGAGATGTCAGCGTGGCATTTTCCGTCCTTGTGTCGGACGTCGGAGGAATcgg > 2:306787/1‑300 (MQ=255) aTTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAACGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCCGAGTGTCCGTTCAGGCGATCGGCTTCCCGAGTCAGACGACGTCAACGTCGGTGGCTTAGATCCCGCACCAGAA‑G‑CGGCATCCGATCTGGCCAGGCGCCGAGCAGTCCTGCTCCAAACTGTCAGCCAGGCCTTTTCCTTCCTCCTTTGGCACGTCGTCGCAATAGGCCgg > 2:169706/1‑300 (MQ=255) aTTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCACACGGTGCCCGAAAATCCACTCTGCTCAGTGTCCTTTCAGGCGATCGGCCTCCCGATCCAGGCGACGTCAACGCCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGCTATGGCCAGGTTGCGCGCACTCCTCATTCCAGCCTCCAGCACGCCACTTTCCTTCCTCCTGTCGGgcggcgacgacataggccac > 2:169700/1‑286 (MQ=255) gTCAACGGATCCATTGACGGAAAAAAGCTCCTCGACGCCGTCTCCCTCACGGCCTACCCCGGGGACGTGGTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGCGCGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGCCGGTGGCTTAGATCCCGCACCAGCA‑G‑CGCCATCCGATATGGCCACGGTGCGAGCGGTCATTCTTCCAGATGTCAGCGTGGCCTTATCCTTCCTCGCTGGTGACTTCATAGAAATGTGCACGCGGCCGTCGCACAAGGCg > 2:247700/1‑300 (MQ=255) aTTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCAGATGCAGGCGAAGTCAACGTCGGTGGCTGAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGaa < 2:468131/300‑1 (MQ=255) ttGAAGGAAAAAAGCTTCTCGACGCCGTCGCACTCAAGGCCTACCCCGGGGGAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACGCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGGGGTTTAGATCTCGAAACGGCG‑G‑CGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAag < 1:484496/300‑1 (MQ=255) taCGCCGTCGCCCTCAAGGCCTACCCCGGGGAAGTGTTGTGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGTCGAAGTCAACGTCGGTGGCTTATCTTCCGCACCGGCG‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCg < 1:452890/297‑1 (MQ=255) cccTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCACACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCTAGATGGCAGCGTGGGATTTTCTTACCTCGTGTGGGACGTCGTAGAACTGGGCAGGCGGCCGTGGCTGAAGGCGTCAACTCCCGACGAGGGTCAGGAAATCATAGAACCAGCGCGTg > 2:519836/1‑300 (MQ=255) gCCTACCCCGGGGAAGGGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGTTTTATTTTTC‑AAGCAGAAGG‑CGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCAcc < 1:306787/300‑1 (MQ=255) cTCATCGGCACAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTAAGGCGATCGGCGTCCCGATTCAGGCGAAGTCGACGTCGGTTGCTTATATTTC‑AAGCAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTAGCGCACCTTGCCGAACgg < 1:433535/300‑2 (MQ=255) aTCGGCCCAAACGGGGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTAGATCCCGAGAAGGCA‑G‑CGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGaa < 1:519836/300‑1 (MQ=255) ccGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGAAACGAAG‑G‑CGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCa < 1:518031/299‑1 (MQ=255) aaaaTCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATGTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATGACGAGATCATCGAAGCAGAGCATGCCGCCACCTCGGTATCGCACCTTGCCGAACGGGAAATCACCACAGTGCCAcgcgg > 2:367773/1‑300 (MQ=255) atgctgctgAGTGTCCTTGCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGTTTTGTTTTC‑AAGAAGAA‑GACGGCATACGAGATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCgg < 1:30872/297‑1 (MQ=255) gctgAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGCAGCAGCGCTTGCCGCCCCCTCGGTAGCGCCCCTTGCCGAACGTGAAATCACCACACTGTCCGGAGGCGACCGGGCAcg > 2:37932/1‑300 (MQ=255) aGTGTCCTTGCAGGCGATCGTCTTCCCGATTAAGGCGAGTTCAACGTCGGTGTCTTTGTTTCCG‑AGCAAAA‑GACGGCATACGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGt < 1:57309/299‑1 (MQ=255) tCAGGCGATCGGCCTCCCGATTCAGGCGCAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTCCACGATGTCAGCGTGGCATTTCACTTCCCCGTGTGGGACGTCGTAGAACTGGGCATGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGACACAGCGCTTGCCGCCAACCCGGTATCGCACATCGTCGAACGTGAAAGCACCACAATGTAAGGCAGCGCGCCGGCTcgcgcgccctcggcc > 2:240438/1‑289 (MQ=255) gCTTAGTTCCCGCAACGGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGaa < 1:366089/299‑1 (MQ=255) ca‑g‑cGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCCCCTCGGTATCGCACCTTGCCGACCGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCCCGCCTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGAGGGat > 1:60813/1‑299 (MQ=255) ca‑g‑cGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGATGGata > 1:69851/1‑300 (MQ=255) ccGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAACTCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCCACGCAAACCCCCATTGTGCTGTTGGACGAACCAACCACCGCCATGGGAATCAGccaca > 2:20564/1‑299 (MQ=255) | AGCGCTGGTCGGCGGCCCAACATTCTTCATAATGTTGCGCCGAATGATGAAAAAGGGCGTGCACTAAATGGCCATTGTTTCCCTCGACAACGTCACCGTATCCATTGAAGGAAAAAAGCTTCTCGACGCCGTCTCCCTCAAGGCCTACCCCGGGGAAGTGTTGGGACTCATCGGCCCAAACGGTGCCGGAAAATCCACTCTGCTGAGTGTCCTTTCAGGCGATCGGCTTCCCGATTCAGGCGAAGTCAACGTCGGTGGCTTAGATCCCGCAACAGCA‑G‑CGGCATCCGATATGGCCAGGGTGCGAGCAGTCATGCTTCAAGATGTCAGCGTGGCATTTTCCTTCCTCGTGTGGGACGTCGTAGAAATGGGCAGGCGGCCGTGGCAGAAGGCGTCAACCCCCGAAGAGGATCATGAAATCATCGAAGCAGCGCTTGCCGCCACCTCGGTATCGCACCTTGCCGAACGTGAAATCACCACACTGTCAGGCGGCGAGCGGGCACGCGTTGCCTTGTCCCGTGTCCTTGCTCAGCAAACCCCCATTGTGCTGTTGGACGAACCAACAGCCGCGATGGATATCAGCCACC > BA000036/414647‑415230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |