Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP034943 | 1,730,075 | 0 | G | C | 41.8% | 47.8 / 20.3 | 58 | E166Q (GAA→CAA) | rodZ | cell shape determination protein RodZ |
Reads supporting (aligned to +/- strand): ref base G (20/12); new base C (0/23); total (20/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.81e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTC > NZ_CP034943/1729939‑1730211 | cGATTGTAACCCTGACGCATCTGAAAAATCAATTACTCAAATTGAAAGCAAGTACGAAATCCCAAAAGATTAAACGCTAAAAGAGAATAAAAGCAACATCTCTGAAAAAGAGGGAGAAACTAAAATAGAAACgaatgaaa < 2:227490/140‑1 (MQ=255) cGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAGGAGGCAGACACTAAAAAAGAAACgaaagaaa > 2:565992/1‑140 (MQ=255) aTGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGaaagaaaaa > 2:1258926/1‑140 (MQ=255) caacttaaaaaTCAATTACGCAAAGTGAAAGCAAGTACGAAATCCAAAAAGATTCAACGCTAAAATATAAACAAAGCAAAAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAaagaa < 2:1104167/134‑1 (MQ=255) cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa > 2:1257390/1‑140 (MQ=255) tACGCAATTTTAAATCAATGACGAAATCCCATAATATTAATCGGTAAAATATTATCAATGCAACTGTTGTGAAAAAGAGGCATTAACTAAAATAGAAACGAAAGAAAAAGAAGATTAAATAGAAGAAAGCGACAGTGATa < 2:1771702/140‑1 (MQ=255) aaaGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGAGACAGTGATAAACTg > 2:45038/1‑140 (MQ=255) tGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGgaga > 2:1359225/1‑140 (MQ=255) tGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGgaga > 2:130714/1‑140 (MQ=255) tGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGgaga > 2:1182653/1‑140 (MQ=255) aGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTaa > 2:571331/1‑140 (MQ=255) gaaaGTAATAAAGCCAAAAAGATTCAACGCTAAAACACAAACAACGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTaaa < 2:14186/138‑1 (MQ=255) aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa > 2:1393152/1‑140 (MQ=255) aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa > 2:401280/1‑140 (MQ=255) tACTAAACCCAAAAAGTTAAACCGATAAAACACAATAAAACAAACTGCTCTCAAAAAGAGGCACACACTAAAACCCAACCGAAACAAAAACACCATAAAAACCAACACCGCGACAGTGATAAACTGGAGATTAAAGCAAc < 1:622857/140‑1 (MQ=255) tACGAAATCCCAAACGATTAAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACAATAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAAc < 2:929430/140‑1 (MQ=255) tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc > 1:584101/1‑140 (MQ=255) tCCCAAAAGATTCAACGCTAAAAGAGAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt < 2:612373/140‑1 (MQ=255) caaaaaTATCCAACCATAAAACAAAAAAAAACCAACAGCTCTCAAAAACAGCCACAAAATAAAAAACAACCCAAACAAAAACAACAAAACAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa < 1:164614/138‑1 (MQ=255) cAAAATATTCAACGATAAAAGATAATAAAAGAAAAAGATATTAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAACAAGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa < 2:187781/140‑1 (MQ=255) cAAAAGTTTCAACGCTAAAACAGAATAAACGCAACCGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACCAAACAAAAATAATATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa < 1:920457/140‑1 (MQ=255) cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAGGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGTTAAAGTGGAGATTAAAGCAACTGGCACTGac > 2:1880505/1‑139 (MQ=255) cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa > 2:591023/1‑140 (MQ=255) cAAAAGATTAAACGATAAAACACAATCAAAGAAACCGATCTCAAAAAGAGCCACACACAAAAAAACAAACCAAAGAAAAACAACATAAAAAACACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa < 1:1484205/140‑1 (MQ=255) aaaaaGATTCAACGATAAAACACAATAAAAGCAACACATCTCAAAAAGAGGAAGACACTAAAAAACAAACGAAACAAAAACAACATAAAAAAGAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa < 1:90331/139‑1 (MQ=255) aaaaaGATTAAACGCTAAAAGACAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAACATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa < 2:60300/139‑1 (MQ=255) aaaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAATAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGTCACAGAAgt > 2:1865634/1‑139 (MQ=255) aaGATTCAACGATAAAACAAAATCAAAGAAACAGATATTAAAAAGAGGAAGACACTAAAAAAGAAACGAAATAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg < 2:247919/140‑1 (MQ=255) ttCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAt > 2:276344/1‑140 (MQ=255) ccAACGCTAAAACACAATCAAAGAAACAGCTCTTAAAAAGAGGCAAAAACAAAAAAACAAACGAAACAAACACACGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt < 2:1653293/139‑1 (MQ=255) ccgatAAAAGACACTCAACCCAACAGCTCTCAAACCGAGCCCCACACCAACACACAACCCAAACAAACAGAAGACAAAACCGACCACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc < 1:565992/136‑1 (MQ=255) actatAAAAGACAATAAAAGAACCAGCTCTTAAAAAGCGGACCACACTAAAAAAGAAACGAAACAAAATGCAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc < 1:927035/136‑1 (MQ=255) aCGCTAAAACATAATCAAACCAACAGCTCTGAAAAAGAGGCACACACTAAAAAATAAACGAAACAAAAAGAACATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc < 2:584101/140‑1 (MQ=255) aCGATAAAAGACAATAAAAGAAACAGCTCTGAAAAAGAGGCACACAATAAAAAAGAAACGAAACAAAAACAAGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc < 1:403805/140‑1 (MQ=255) acaaTCAAACCAACCGCTCTGAAAAAGAGGAACACACTAAAAAACAAACCAAACAAAAAGAAGATAAAAACCAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa < 1:977537/138‑1 (MQ=255) acaaTCAAACCAACACCTCTCAAAAAGAGGCAGACACTAAAAAACAAACCACACAAAAACAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa < 2:1711735/138‑1 (MQ=255) acaaTAAAAGCAACACAACTCAAAAAGAGCAACAAAATAAAAAACAAACCAAACAAAAACAAGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa < 2:1711526/138‑1 (MQ=255) aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt > 1:1797181/1‑140 (MQ=255) aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAAATGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt > 2:497174/1‑140 (MQ=255) aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGAAAAACTGGAGATTAAAGCAACTCGCACAGAAGCGTCATTAACGAATTATCaact > 2:1134218/1‑138 (MQ=255) aaccAACAGCTCTGAAACAGAGGAAGAACAAAAAAAATAAACGACACAAAAACAAGATAAACCACACGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTTTCCg < 2:996905/137‑1 (MQ=255) aCAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCt > 2:1696851/1‑140 (MQ=255) cAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTg > 2:36539/1‑140 (MQ=255) cAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAATGGCACTGAAGGGTCATTAACTACTTATGAAGTGTCCGGCGCTg > 2:1293948/1‑140 (MQ=255) agagGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc < 2:972987/140‑1 (MQ=255) agGCAGACACTAAAAAATAAACGAACTAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTc < 2:575906/140‑1 (MQ=255) agGAAGACAGTAAAAAATAAACGAAACAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTc < 2:1095907/140‑1 (MQ=255) caatAAAAAATAAACGAAACAAAAAGACTATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa < 1:1360300/137‑1 (MQ=255) caatAAAAAACAAACCAAACAAAAACACGATAAAACAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa < 1:764318/137‑1 (MQ=255) aacTAAAAAAGAAACGAAAGAAAAAGACGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa < 2:1906634/139‑1 (MQ=255) aacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa < 2:1797181/139‑1 (MQ=255) tAAAAAATAAACCAAAAAAAAAGAAGATAAAAACCAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg < 1:577065/140‑1 (MQ=255) aaaaaGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa > 2:592309/1‑140 (MQ=255) taaaCCAAACAAAAATAAGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 1:130714/139‑1 (MQ=255) gAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 1:1359225/140‑1 (MQ=255) gAAACCAAACAAAAACAAGAAAAAAAAGACTAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 1:162247/140‑1 (MQ=255) caaaCGAAAGAAAAAGAAGATAAAAACGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 1:1940142/139‑1 (MQ=255) caaaCGAAACAAAAACAATCTAAATAAGTAGAAAGCGACAGTGATACCCTGGAGATTAAAGCACCTGGCCCTGTAGGGTCATTAACGACGTATCAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 2:354024/139‑1 (MQ=255) caaaCCAAACAAAATTAATATAAAAAATAACAACGCGACATTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 1:1182653/139‑1 (MQ=255) caaaCCAAACAAAAACAAGAAAAAAAACAACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 1:1258926/139‑1 (MQ=255) aaaccgtaagaaaacgaaGATAAATACCAAGAACGAGACAGTGATAACCTGGAGATTAAAGCACCTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGGTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 2:636038/133‑1 (MQ=255) aaaaCAAAAGAAAAATAAGCTAAAAATGACGAAAGAGACAGTGATAAACTGGAGATTAAAGCAACTGGCAATGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa < 1:1865634/139‑1 (MQ=255) aaataaaatcaaTATAAAAACAAACACCGCGACAGTGATAAACTTGAGATTAAAGCAACTGGCACTGATGGGTCATTAAAGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGTACTGAAGGCATCAGACAGTTc < 1:497174/140‑1 (MQ=255) | CGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTC > NZ_CP034943/1729939‑1730211 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |