Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,5800AG42.4% 123.8 / 238.9 170I274T (ATA→ACA) EO946_RS16995IS3 family transposase
Reads supporting (aligned to +/- strand):  ref base A (54/44);  new base G (37/35);  total (91/79)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 171 total aligned reads displayed.

GGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTC  >  NZ_CP034943/3285445‑3285719
                                                                                                                                       |                                                                                                                                           
 gCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCt                                                                                                                                        <  2:636602/140‑1 (MQ=34)
 gCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCt                                                                                                                                        <  2:1874523/140‑1 (MQ=34)
 gCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCt                                                                                                                                        <  1:951182/140‑1 (MQ=34)
                      tCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGc                                                                                                                   <  2:269657/140‑1 (MQ=34)
                         tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTa                                                                                                                >  2:930372/1‑140 (MQ=34)
                               tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                                                                                                          >  1:932312/1‑140 (MQ=40)
                               tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                                                                                                          >  2:72240/1‑140 (MQ=40)
                               tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                                                                                                          >  2:472204/1‑140 (MQ=40)
                               tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                                                                                                          >  2:1631402/1‑140 (MQ=40)
                               tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                                                                                                          >  1:930281/1‑140 (MQ=40)
                               tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCAAAGACAAAGTCTGTGTCCTGCTTATCCCCa                                                                                                          >  2:476236/1‑140 (MQ=255)
                               tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGCAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                                                                                                          <  2:1328332/140‑1 (MQ=255)
                                    tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt                                                                                                     >  2:1614932/1‑140 (MQ=255)
                                    tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt                                                                                                     >  1:1234466/1‑140 (MQ=255)
                                     ttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa                                                                                                    <  1:264038/140‑1 (MQ=255)
                                      tgtgTTAATAGCTTTCTGATACTAGTAAGATGTAAACACAGAACCTTGGTCACTATGTAACACGCAGTTCTAAGGCAGTGTTTTCAGCTGATTAAGTATGTCTAAGACAAAGTCTGAGTCCTGCTTATCCCCAATCGTaa                                                                                                   >  2:791796/1‑140 (MQ=14)
                                           tAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                                              <  1:798832/140‑1 (MQ=255)
                                           tAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                                              <  1:1788515/140‑1 (MQ=255)
                                           tAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                                              <  2:1015472/140‑1 (MQ=255)
                                            aacaGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCaa                                                                                             >  1:418486/4‑140 (MQ=255)
                                            aacaGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCaa                                                                                             >  1:1966639/4‑140 (MQ=255)
                                             aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt                                                                                            <  2:1192669/140‑1 (MQ=255)
                                             aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCAGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt                                                                                            >  2:603508/1‑140 (MQ=255)
                                                  tttCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                                       >  1:1936377/1‑140 (MQ=255)
                                                  tttCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                                       <  2:932312/140‑1 (MQ=40)
                                                  tttCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                                       <  1:277108/140‑1 (MQ=40)
                                                    tCTGATACTCGTAAGATGTATACACAGATCCTTGGTCACTATTTAACACGCAGTTCTAAGTCAGTGTTGGCAGCTGATTAAGTATGTATAAGACAAAGTCTGTGTCCTGCTTATCACCACTCGTAAAAGCAATCACTtct                                                                                     <  1:1564286/140‑1 (MQ=11)
                                                    tCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                                                                                                                       <  1:682372/106‑1 (MQ=32)
                                                    tCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                                              <  1:137966/131‑1 (MQ=40)
                                                    tCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                                              >  2:137966/1‑131 (MQ=40)
                                                       gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                                  <  1:580458/140‑1 (MQ=255)
                                                       gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                                  <  2:1077994/140‑1 (MQ=40)
                                                       gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                                  <  2:1110101/140‑1 (MQ=40)
                                                       gATACTCGTAAGATGTAGACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                                  <  2:1110172/140‑1 (MQ=255)
                                                           cTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGt                                                                              >  2:926811/1‑140 (MQ=40)
                                                           cTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGt                                                                              >  2:165491/1‑140 (MQ=40)
                                                             cGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtat                                                                            <  1:1631402/140‑1 (MQ=40)
                                                             cGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtat                                                                            <  1:776951/140‑1 (MQ=40)
                                                               tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                          <  2:1144970/140‑1 (MQ=40)
                                                                  gATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATc                                                                       <  2:756440/140‑1 (MQ=255)
                                                                      tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAATACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAat                                                                   >  1:1432466/1‑140 (MQ=255)
                                                                      tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGATAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAat                                                                   >  2:700440/1‑140 (MQ=255)
                                                                       ataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAata                                                                  <  2:1016693/140‑1 (MQ=255)
                                                                        taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAatat                                                                 >  2:381934/1‑140 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCCGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAACTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCCTTGTATAAATCTAATATACTgg                                                            >  1:1800435/1‑140 (MQ=11)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                            >  1:1005307/1‑140 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                            <  2:1462499/140‑1 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                            <  2:1200941/140‑1 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                            >  2:1189253/1‑140 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                            >  2:878880/1‑140 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                            >  2:769289/1‑140 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                                  >  1:587959/1‑118 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                                  <  2:587959/118‑1 (MQ=255)
                                                                             aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                            >  1:49322/1‑140 (MQ=255)
                                                                              gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCACCATTGTATAAATCTAATATACTGGa                                                           >  1:1498535/1‑140 (MQ=11)
                                                                               aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                          >  1:536200/1‑140 (MQ=255)
                                                                               aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                          >  2:1231181/1‑140 (MQ=255)
                                                                               aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                          >  1:1824935/1‑140 (MQ=255)
                                                                               aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                          <  1:1598768/140‑1 (MQ=255)
                                                                                aCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaaa                                                         >  1:355702/1‑140 (MQ=255)
                                                                                   ttGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGATAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGt                                                      <  1:700440/140‑1 (MQ=255)
                                                                                    tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCGTATCCCCACTCGTCCAAGCAATCACTTCTCCAGTGTCTAACTCTAATCTACTGGAAAGGtc                                                     >  2:25045/1‑139 (MQ=11)
                                                                                    tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTa                                                     >  1:1155309/1‑140 (MQ=255)
                                                                                     ggTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTAc                                                    >  2:1675804/1‑140 (MQ=255)
                                                                                     ggTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                                       >  1:1957580/1‑105 (MQ=255)
                                                                                     ggTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                                       <  2:1957580/105‑1 (MQ=255)
                                                                                         aCTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAAtt                                                >  1:1323388/1‑140 (MQ=255)
                                                                                           tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGt                                              >  1:1732563/1‑140 (MQ=255)
                                                                                           tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGt                                              >  2:1500992/1‑140 (MQ=255)
                                                                                            aTGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATAATGGAAAGGTACAATTGtt                                             >  1:519914/1‑140 (MQ=255)
                                                                                             tGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGt                                                      >  1:1027597/1‑130 (MQ=255)
                                                                                             tGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGt                                                      <  2:1027597/130‑1 (MQ=255)
                                                                                             tGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGttt                                            <  1:189918/140‑1 (MQ=255)
                                                                                                    agCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCa                                     <  1:834218/139‑1 (MQ=255)
                                                                                                     gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                    >  2:155056/1‑140 (MQ=255)
                                                                                                     gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                    >  1:638094/1‑140 (MQ=255)
                                                                                                     gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                    >  2:348680/1‑140 (MQ=255)
                                                                                                      cAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                                   <  1:631249/140‑1 (MQ=255)
                                                                                                      cAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                                   <  1:1660419/140‑1 (MQ=255)
                                                                                                       aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTTTACTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATAGTATACATCTAATATACTTGAAAGGTACAATTTTTTCTGTCAATaa                                  >  1:1170214/1‑140 (MQ=14)
                                                                                                       aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                  >  1:749084/1‑140 (MQ=255)
                                                                                                       aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                  >  1:206413/1‑140 (MQ=255)
                                                                                                       aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAtca                                  >  1:1827220/1‑138 (MQ=255)
                                                                                                        gTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAg                                 <  1:1098236/140‑1 (MQ=255)
                                                                                                         ttCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                >  1:1396312/1‑140 (MQ=255)
                                                                                                           ctcAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                              <  2:370024/140‑1 (MQ=255)
                                                                                                             cAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCaaa                            <  1:538702/140‑1 (MQ=255)
                                                                                                              aGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                                     >  2:202631/1‑98 (MQ=255)
                                                                                                                 cAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGGATACATCTAATATACTGGAAAGGTACAATTGTTTGTGTCCATAAGGCAAATAtg                        >  2:866259/1‑140 (MQ=11)
                                                                                                                   gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtataa                                                                          >  1:1308908/1‑87 (MQ=11)
                                                                                                                   gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                      >  1:1911324/1‑140 (MQ=255)
                                                                                                                        ggtaGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTc                 >  2:656987/4‑140 (MQ=255)
                                                                                                                              tgatgaAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAATGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc           >  2:913861/1‑140 (MQ=255)
                                                                                                                              tgatgaAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc           >  2:311833/1‑140 (MQ=12)
                                                                                                                              tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCCTTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc           >  2:1427092/1‑140 (MQ=255)
                                                                                                                              tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc           >  2:648701/1‑140 (MQ=255)
                                                                                                                              tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc           >  1:438956/1‑140 (MQ=255)
                                                                                                                               gATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACt          <  2:1911324/140‑1 (MQ=255)
                                                                                                                                aTTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTa         >  2:1004857/1‑140 (MQ=255)
                                                                                                                                       aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc  >  1:1217013/1‑140 (MQ=40)
                                                                                                                                       |                                                                                                                                           
GGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTC  >  NZ_CP034943/3285445‑3285719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: