Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9700GT32.3% 178.0 / 39.5 131Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (32/56);  new base T (42/0);  total (75/56)
Fisher's exact test for biased strand distribution p-value = 4.62e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 135 total aligned reads displayed.

CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108
                                                                                                                                            |                                                                                                                                           
ctATTGTAAGGGAAACTGCAGCCTATAAAAAGACCTGCCAAGTTAAAGTTAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGAACAGAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTg                                                                                                                                             <  1:159970/140‑1 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTg                                                                                                                                             >  1:1957327/1‑140 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTg                                                                                                                                             <  1:532230/140‑1 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTg                                                                                                                                             <  1:1274917/140‑1 (MQ=255)
 tATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGc                                                                                                                                            >  2:621838/1‑140 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCa                                                                                                                                           <  1:1018685/140‑1 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCa                                                                                                                                           <  1:1188954/140‑1 (MQ=255)
   ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAGTC‑ATTTTTTTCaa                                                                                                                                          >  1:1523616/1‑140 (MQ=255)
   ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTC‑ATTTTTTGCaa                                                                                                                                          >  2:1707880/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAaaat                                                                                                                                     >  1:1196688/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTA‑ATTTTTTTCAAAatat                                                                                                                                     >  1:1713768/1‑140 (MQ=255)
         gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAGTC‑ATTTTTTGCCAAATAtt                                                                                                                                    >  2:1244591/1‑140 (MQ=255)
         gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAAATA‑ATTTTTTTCAAAATAtt                                                                                                                                    >  2:1676999/1‑140 (MQ=255)
           gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTC‑ATTTTTTTCAAAAAAtttc                                                                                                                                  >  1:265130/1‑138 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTA‑ATTTTTTTCAAAATATTGCTc                                                                                                                                >  2:1018685/1‑140 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGGCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTCCAATC‑ATTTTTTTCAAAATATTGGTc                                                                                                                                >  1:1107962/1‑140 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTcaca                                                                                                                             <  1:538047/140‑1 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTcaca                                                                                                                             <  1:1467531/140‑1 (MQ=255)
                              aGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGt                                                                                                              <  2:17006/140‑1 (MQ=255)
                               gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATCCTCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTGTTTGCAAAATATTGCTCACA‑GGGTTTTTCACgggt                                                                                                             >  2:318524/1‑138 (MQ=255)
                               gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTTCTCACA‑TGGTTTTTCACGGtt                                                                                                             >  2:65861/1‑140 (MQ=255)
                                   tccccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTtct                                                                                                         <  2:534339/138‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTtct                                                                                                         <  2:1614502/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATAGTAGCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAGTTTG‑ATTTTTTTCAAAATATTTCGGACA‑AGGTTTTTAAAGGTTTTCTCa                                                                                                       >  1:170069/1‑140 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCa                                                                                                       <  1:578316/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCa                                                                                                       <  1:1262574/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCa                                                                                                       <  2:555632/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAGTC‑ATTTTTTTCAAAATATTGCT‑ACACTGGTTTTTCACGGtgtttgca                                                                                                       >  1:1159065/1‑136 (MQ=255)
                                     cccAGTAAAACGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑GGGTTTTTCACGGTTTTCGCa                                                                                                       <  2:1681169/140‑1 (MQ=255)
                                         gTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGa                                                                                                   <  1:189112/140‑1 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTTCAAAATATTTCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                             >  1:1618631/1‑140 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCAATC‑AATTTTTGCAAAAAATTTCTCACA‑AGGTTTTTCACGGTTTTATCAAAGATaaaaa                                                                                             >  1:444309/1‑138 (MQ=255)
                                                   agacagttattaTACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCCAAATATTGCTCACA‑TGGTTTGTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  1:1690552/134‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  1:1904439/140‑1 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTA‑AATTTTTTCAAAAAATTTGTCACA‑TGGTTTTTAACGGTTTTGTCACTGTTAAAAAATTAt                                                                                        >  1:1373237/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTC‑ATTTTTTGCAAAATATTGCTCACA‑AGGTTTTTAACGGGTTTCTGACTGATAAACAATTAt                                                                                        >  2:293325/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTA‑ATTTTTTTCAAAAAAATTCTCACA‑TGGTTTTTAACCGTTTTATCACTGATAAAAAATGAt                                                                                        >  1:448828/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATA‑ATTTTTTTCAAAAAAATGCTCAAA‑AGGTTTTTAACGGTTTTATAACTGTTAAAAAAAGAt                                                                                        >  1:542740/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTC‑ATTTTTTGCAAAATATTGATAACA‑ATGTTTTTCAAGGTTTTATCACTGATaaaaaaatat                                                                                        >  1:1126907/1‑136 (MQ=255)
                                                     tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                       <  1:1794942/140‑1 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTA‑ATTTTTTGCAAAATAATTCGCACA‑TTGTTTTTAACGGGTTTATCAATTAAAAAAAAGTATTAACCaaaaac                                                                             >  1:317013/1‑136 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTC‑ATTTTTTGCAAAATATTTCTAACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTAACCAATaac                                                                             >  2:1203504/1‑138 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTA‑ATTTTTTTCAAAAAATTTCTCACA‑AGGTTTTTAACGGTTTTCTCACTGATAAAAAAAGATTcaacaaaaac                                                                             >  1:186462/1‑136 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACTGCTTGCGTCCGGTCGTTTACAATCAATTTTTTCCAAAAAATT‑AGCACA‑TGGTTTTTCACGGTTTTGGAACGGGTAAAAAATGATTCAACAATAcccc                                                                           >  1:1954028/1‑138 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTA‑ATTTTTTTCAAAAAATTTCTCACA‑AGGTTTTTCACGGTTTTCTCAAAGATAAACAAAGATTAACCAAAAAcgc                                                                           >  1:1425725/1‑140 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATA‑AATTTTTTCAAAAAAATTCTCACA‑AGGTTTTTAAAGGTTTTATCAATGATAAAAAAATATTAACCaaaaaccc                                                                           >  1:1425397/1‑134 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCAGGTCGTGTACATTC‑ATTTTTTGAAAAATATTGCTCACA‑AGGTTTTTAACGGTTTTATCACTGATAAAAAATGTTTGACCAAAACagc                                                                           >  1:490543/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTTCAAAATATTGCTAACA‑TGGGTTTTAAACGTTTTCTCACTGAAAAACAAAGATTCACCAATAACGCgg                                                                         >  2:812281/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTTCAAAAAATTGATCACA‑TTGTTTTTCACGGTTTTATCACAGATAAAAAAATAATAACCAATAACCCgg                                                                         >  1:1438532/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                       <  1:426251/126‑1 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                       >  2:426251/1‑126 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑AGGTTTTTCACCGTTTTCTAACTGATAAAAAATTATTCACCAATACCGCgg                                                                         >  1:788101/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTA‑ATTTTTTTCAAAATATTGCTCACA‑TGGTTTTTAAACGTTTTCTAACTGATAAAAAAAGATTCACCAAAAACGCgg                                                                         >  2:910709/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATC‑ATTTTTTTCAAAATAATGCTCACA‑AGGTTTTTAAAGGTTTTGTAACTGATAAAAAATTATTCACCCAAACcgccg                                                                         >  1:1020313/1‑138 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTC‑ATTTTTTGCACAATAATGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAAAAATTATTCACCAATACCGCgg                                                                         >  2:1156923/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTA‑ATTTTTTTCAAAATATTGGTCACA‑TTGTTTTTAACGGTTTTCTCACTGATAAACAAAGATTCACCAAAACCCCgg                                                                         >  1:66705/1‑140 (MQ=255)
                                                                   aTCTCATGACAACCCAGACATTTTTAATGGCGACCACAACGGCTTGCGACCGGTCGATTACAATC‑AATTTTTGAAAAATATTGCTCACA‑TGGTTTATAACGGTTTTCTCACAGATAAACAAAGTTTCACCAATaaaccgt                                                                         >  2:871336/1‑134 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCTACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATAGCTCACA‑TGTTTTTTCACGGTTTTATCACAGATAAACAATGATTCAACAATAAcgcgct                                                                        >  2:1871597/1‑138 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTTCAAAATATTTCTCAAA‑TTGTTTTTAAAGGTTTTATCACTGATAAAAAATGATTAAACAAAAACGCGGt                                                                        >  2:1660208/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGt                                                                        <  1:1290670/140‑1 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑AATTTTTTCAAAAAATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAAAGAATAACCAAAACCCCGGt                                                                        >  2:1394248/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑AATTTTTGCAAAATATTGCTCACA‑AGGTTTTTAACGGTTTTCTCACTGATAAACAAAGATTCACCAAAAACCCGGt                                                                        >  2:1710624/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑AATTTTTGCAAAATATTGCTAACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGAATCACCAATAACCCggg                                                                        >  2:1248944/1‑139 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTA‑ATTTTTTTCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTAACCAAAAACCCGGt                                                                        >  2:476325/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTA‑ATTTTTTTCAAAATATTGCTAAAA‑TGGTTTTTAAAGGTTTTATCACTTATAAAAAATGATTAACCAATAACGCGGt                                                                        >  2:1049604/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTA‑ATTTTTTGCAAAATATTTCTCACA‑TGGTTTTTAACCGTTTTATCACTGATAAAAAAATATTCACCAATACCGCCGt                                                                        >  2:1219945/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTA‑AATTTTTTCAAAAAATTTCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTAACCAATACCGCGGt                                                                        >  2:520359/1‑140 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑AATTTTTGAAAAATAATGGTCACA‑AGGTTTTTCAAGGTTTTATAAAATATAAAAAAAGATTAACCAATCCCCCGGTTGCtttttac                                                              >  1:1892577/1‑138 (MQ=255)
                                                                                ccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGt                                                            <  1:621838/140‑1 (MQ=255)
                                                                                  aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCt                                                          >  2:346396/1‑140 (MQ=255)
                                                                                    ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTG‑ATGTTTTGCAAAATATTTCTAACC‑TGGTTTTTAACGGTTTTTTCAAAGATAAAAAATGATTCACCAAAACAGCGGTTTGTTTTTCCGTCTGc                                                        >  1:260189/1‑140 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCa                                                       <  2:1107962/140‑1 (MQ=255)
                                                                                      aTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCaa                                                      <  2:1535898/140‑1 (MQ=255)
                                                                                       tttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAg                                                     >  2:595078/1‑140 (MQ=255)
                                                                                        gtttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑CTTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                    <  2:1316883/139‑1 (MQ=255)
                                                                                             aTGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTC‑ATGTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTg                                               <  1:226400/140‑1 (MQ=255)
                                                                                               ggCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATAGTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgca                                             <  1:758767/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:1049604/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  2:1540289/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTAACTGCTAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:1190444/140‑1 (MQ=255)
                                                                                                 cGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                           <  2:1957327/140‑1 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTTCAAAAAATTTCTCACA‑AGGTTTTTAACGGTTTTATACCTGATAAAAAAAGATTCACCAATAACGCGGTTGGTTTTTACGTCTGCAAGCATCTgcagca                                          >  1:524957/1‑140 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTTCAAAAAATTGCTCACA‑TGGTTTTTCACGGTTTTGTCACTGATAAAAAATGATTAACCCAAAACGCGGGTTCTTTTTGCCTCGGCAAGCATATggagca                                          >  1:1294481/1‑140 (MQ=255)
                                                                                                   aCCACAACGGCTTGCGTCCGGTCGTGTTCATTG‑ATTTTTTTCAAAAAAATTGTCACA‑AGGTTTTTCACGGTTTTTTGACTGTTAAAAAAATATTAACCAAAAACGCGGTTTCTTTTTGCGTCTGCAAGCAACTTCAGCAc                                         >  1:1612541/1‑140 (MQ=255)
                                                                                                       caACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                     <  1:451696/140‑1 (MQ=255)
                                                                                                       caACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                     <  1:451647/140‑1 (MQ=255)
                                                                                                                gCGTCCGGTAGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgc                            <  2:820027/140‑1 (MQ=255)
                                                                                                                 cGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                           <  2:1624680/140‑1 (MQ=255)
                                                                                                                    ccGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                        <  1:910709/140‑1 (MQ=255)
                                                                                                                    ccGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                        <  2:1826365/140‑1 (MQ=255)
                                                                                                                    ccGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                        <  2:84288/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:265130/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:1797417/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:1797212/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:1243411/140‑1 (MQ=255)
                                                                                                                           tttACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  1:1822724/140‑1 (MQ=255)
                                                                                                                           tttACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  1:1220777/140‑1 (MQ=255)
                                                                                                                                attc‑attTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa            <  1:851232/140‑1 (MQ=255)
                                                                                                                                     attTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTCATgg        >  1:387585/1‑140 (MQ=255)
                                                                                                                                     attTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTAACATTCCCGCCGAGGTAAAATATGTAATgg        >  1:341415/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTC‑ATTTTTTGCAAAATATTGCTCACA‑TGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑