Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,0510TA28.1% 153.2 / 24.8 83intergenic (+224/‑48)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base T (29/30);  new base A (23/0);  total (52/31)
Fisher's exact test for biased strand distribution p-value = 4.47e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACC  >  NZ_CP034943/176912‑177190
                                                                                                                                           |                                                                                                                                             
aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                                               <  2:1878182/140‑1 (MQ=255)
aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                                               <  1:463180/140‑1 (MQ=255)
  cTTGCGTATTTGATCAAAATTTAATATGATACTCTTTTTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                                                                             <  1:1618845/140‑1 (MQ=255)
  cTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTACCGTAAAATAAATAAACCTCCCAATaa                                                                                                                                             >  2:67509/1‑139 (MQ=255)
    tGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATacaa                                                                                                                                           >  2:1443657/1‑140 (MQ=255)
     gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTACCGTAAAATAAAGAAAGCTCCCAAAacaaa                                                                                                                                          >  2:1398567/1‑139 (MQ=255)
     gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATacaac                                                                                                                                          >  2:1018464/1‑140 (MQ=255)
     gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTAATTTGATTTATAACGTAAAATAAATAAAGCAGACAATacaac                                                                                                                                          >  2:1122718/1‑140 (MQ=255)
          tttGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTACCGTAAAATAAAGAAAGCTCCCAATAAAACAAATa                                                                                                                                     >  2:1459887/1‑140 (MQ=255)
           ttGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTATACACTAAAATAAAGAAATCTACCAAAACAACAAATAc                                                                                                                                    >  2:814173/1‑140 (MQ=255)
             gATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCATTTTCTCCGGTCGTTTTCCTTTCATTTATACCGTAAAATAAAGAAAACTCCCCATAAAAAAAATACgg                                                                                                                                  >  2:980048/1‑140 (MQ=255)
              aTCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTAAATTATACCGTAAAATAAAAAAATCTCCCAAAAAAAAAAAAAAGGt                                                                                                                                 >  1:475021/1‑140 (MQ=255)
                                aCTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTaaa                                                                                                              <  1:1268515/140‑1 (MQ=255)
                                aCTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTaaa                                                                                                              <  1:1826477/140‑1 (MQ=255)
                                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAgg                                                                                                          <  2:1510057/140‑1 (MQ=255)
                                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAgg                                                                                                          <  2:1008478/140‑1 (MQ=255)
                                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTTATTTCTACCCTAAAAAAAAGAAAGCACCCAAAAAAACAAAAAAAGGGCGGCATTTTTTTTTTAAAAAg                                                                                                           >  1:552544/1‑140 (MQ=255)
                                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAATTTTTGCCGTAAAATAAAGAAAAAGACCAAAAAAAAAAATAAGGTGGGGAATTATTTTTTtaaaatt                                                                                                          >  1:673243/1‑138 (MQ=255)
                                    tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTCATTTCTCCCGTAAAATAAACAAACCACACAAAACAACAAAAAAGGTGCGGATTTATTTTTTAAAAAgg                                                                                                          >  2:189593/1‑140 (MQ=255)
                                    tttGTATAATAACTCCTTGCCTCAAAAAAACATACTCAACGTGTCCTCTGTATCTCCGGTCGTGTTTCTTTAATTTGAGCCGTAAAAAAAATTCTCCGGCCAAAACAACAAAAAAGGGGGGGCTTTATTTTTTtaaaaag                                                                                                          >  1:552714/1‑138 (MQ=255)
                                     ttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                                                                         <  1:1166484/140‑1 (MQ=255)
                                     aggTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                                                                         <  1:927341/138‑1 (MQ=255)
                                      tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTGTACCGTAAAAAAAATAAATCTCCCAATAAAAAAAAAACCGGGGGGATTTATTTTTTAAAAAGGAg                                                                                                        >  1:1266199/1‑140 (MQ=255)
                                      tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTATACCCGAAAAAAAAGAAAACACCCAAAAAAAAAAAAACGGTGCGGATTTATTTTTTAAAAAgtat                                                                                                        >  1:1676671/1‑137 (MQ=255)
                                      tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGGGTTAAATTATACCCGAAAAAAAAAAAAACAGCCACAACAACAAAAAACGTGGGGATTTATTTTTTAAAAAagag                                                                                                        >  1:685661/1‑140 (MQ=255)
                                                cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTTTACCGTAAAAAAAATAAAGCTCCCAAAAAAAAAAAAAACGTGCGGATTTATTTTTTAAAAAAGAGTAGTtttaat                                                                                              >  1:1400568/1‑138 (MQ=255)
                                                cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAATTTCTCCCGTAAAAAAAAGAAATCTCCCAAAACAACAAAAAACGTGGGGGTTTATTTTTTAAAAATGAGTCGTGTTAtt                                                                                              >  1:462958/1‑140 (MQ=255)
                                                cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTGATTTTTCCCCTAAAATTAATAAAGCGCCCAATACAACAACTACGGTGGGGGTTTGTTTTTTTAAAAAGAGTCGtttttat                                                                                              >  1:1190665/1‑135 (MQ=255)
                                                cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTATCCGGTCGTTTTCCTTTCATTTCTCCCCTAAAATAAAGAAATCTCCCAAAAAAACAAATACGGGGCGGCTTTATTTTTTAAAAAGGAGTCGTGAGAtt                                                                                              >  2:1265168/1‑140 (MQ=255)
                                                  ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTATCCCGTAAAATAAATAAAGCTCCCAAAACAAAAAATACGGTGCGGATTTTTTTTTTTAAAAGGTGTGGTTAttttgt                                                                                            >  2:927341/1‑138 (MQ=255)
                                                  ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTCATTTCTCCCGTAAAAAAAAGAAAGCTCCCAATACAAAAAATTCGGGGGGGATTTAATTTTTAAAAAGTTGTCGTTTTAttgt                                                                                            >  2:1650863/1‑138 (MQ=255)
                                                  ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCAGTTCATTTGTCACGTAAAATAAAGAAACCTGCCCAAAAAAAAAAAACGGGGGGGGTTTATTTTTTAAAAAAGAGTGGGTATATTTg                                                                                            >  2:1650893/1‑140 (MQ=255)
                                                  ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGGTTTGCTTTCTGCCGGAAAATAAAGAAAGCGCCCAAAACAAAAAAAAAGGGGGGGGGTTATTTTTTTAAAAGGAGTGGTgattattt                                                                                            >  1:1257961/1‑135 (MQ=255)
                                                  ccTTGCCTCAAAACAACATACACAACGTGTCCTCTTTTTCTCCGGTCGTGTGGGTATGAATTAAACCCTAAAATAAAGAAATCGCCCAAAACAAAAACTACGGTGCGGCGTTATTTTTTTATAAGGTGTCTGTTTAtttt                                                                                            >  1:380533/1‑139 (MQ=255)
                                                    ttGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCGTTTATTTGTCCCGTAAAATAAATAAATCACCCCAAAAAAAAAAAAAGGTGGGGGTTTTTTTTTTTTAAATGTGTGGTTTTAttttta                                                                                          >  1:954940/1‑137 (MQ=255)
                                                     tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTGTCACGTAAAAAAAATAAAGCTCCCCAAAAAAAAAATACCGTGCGGGTTTTTTTTTTAAAAATATGTCGTGATAttttgat                                                                                         >  1:1416744/1‑136 (MQ=255)
                                                     tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATAAGGTGCGGATTTATTTTTTAAAAAGTAGTGGTTATATTTGaaa                                                                                         >  2:393144/1‑139 (MQ=255)
                                                     atccTCAAAACAACATACTCAAATTTTCATAATTTTATCAGCTCGTTTTACTTTCATTTCTCCCCTAAAATAAAGAAAGATACCAAAAAAAAAAAAAAGGTGCGGATTTATTTTTTAAAAATGAGTCGTTATATTTTaat                                                                                         >  2:367830/3‑140 (MQ=255)
                                                     atccTCAAAACAACATACTCAAATTGTCCTCATTTTAACCGGTCGTGTTCCTTTGATTTATCCCCTAAAAAAAAGAAAGCAACCAATAAAAAAAAAAAGGTGCGGATTTATTTTTTAAAAATGAGTGGTTATATTTTaaa                                                                                         >  2:898285/3‑139 (MQ=255)
                                                       ccTCAAAAGATCATACTCAACGTGTCCTTTTTTTCTCCGTTCGTTTTACTTTCATTTCTGACTTAATATAATGATATCTCCCAATACACCATATTCTGTTCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTgtttaa                                                                                       <  2:1267318/140‑6 (MQ=255)
                                                           aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTCCTTTCTGCCGTAAAAAAAAGAAAGCGCCCAATAAAACAAATAGGGGGGGGGTTTATTTTTGTAAAAGTAGGGGTTATAATTTGataataat                                                                                  >  1:369806/1‑138 (MQ=255)
                                                             aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                <  2:475021/140‑1 (MQ=255)
                                                             aacaacAGACTCCACGTGTCCTCTTTGTCTCCGGTCGTCTTCCTTACATTTCTCCCGTAAAATAAACAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                <  2:1962782/140‑1 (MQ=255)
                                                                     aCTCAACGTGTCCTCTTTTTCTCCTGTCGTTTTACTTTAATTTCTACCGTAAAAAAAAGAAATCTCCCAATAAAACAAAAAAGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATATTTTTAAAATaa                                                                        >  2:1452114/1‑140 (MQ=255)
                                                                     aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTGTACCGTAAAAAAAAGAAAGCTCCCAATAAAAAAAATAAGGGGCGGATTTATTTTTTAAAAAGGAGTCGGTATAATTGAAAAATAGTTTTAAATTaa                                                                        >  2:1540458/1‑140 (MQ=255)
                                                                     aCTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCAATTATCCCCTAAAAAAAATAAAGCTCCCAAAACAACAAAAACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTGTTTTTGTAAAATAGTTTTAAATTaa                                                                        >  2:238320/1‑140 (MQ=255)
                                                                        cAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAagc                                                                     <  1:1839826/140‑1 (MQ=255)
                                                                         aaCGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTCATTTCTCCCGTAAAATAAATAAATCTCCCAATAAAACAAAAACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTTAAAATAGTTTTACATTAAagca                                                                    >  2:493983/1‑140 (MQ=255)
                                                                         aaCGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGAATTATTTTTTTAAAAGGAGTCGTTATATTTGAATTATATTTTTAAATTAAagca                                                                    >  2:419146/1‑140 (MQ=255)
                                                                             tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAACAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                <  1:1907325/140‑1 (MQ=255)
                                                                             tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                <  2:823581/140‑1 (MQ=255)
                                                                             tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                <  2:1010868/140‑1 (MQ=255)
                                                                             ggtCCTCTTTGACACCGGTCGTTGTCCGGTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAACAGGAGTCGTTATATTTGAATAATAGTTTTACATTATAGCAGCaa                                                                <  2:1730103/139‑1 (MQ=255)
                                                                              gtCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTGTGCCGTAAAATAAATAAAGCTCCCAATAAAACAAATACGGTGGGGGTTTTTTTTTTTAAAAGGAGTGGGGTTTTTTTTATAATATTTTTTAAATAAATGAGCAAt                                                               >  1:457825/1‑140 (MQ=255)
                                                                                 ctctTTTTCTCCGGTCGTTTTGCTTTCATTTTTCCCGGAAAATAAAGAAATCTCCCAAAACAAAAAATAACGGGCGGCTTTTTTTTTTAAAAAGGATTGGTTTTATTTTTATAAAAGTTTTTAATTAAAACAGCAATGGt                                                            >  2:667034/1‑140 (MQ=255)
                                                                                 ctctTTTTCTCCGGTCGTTTTACTTTAATTTCTCCCGTAAAATAAATAAAGCTCCCAAAAAAACAAAAAAGGGGGGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTGATAATATTTTTTAAATAAATAAGCAAAGGt                                                            >  2:651836/1‑140 (MQ=255)
                                                                                 ctctTTTTCTCCGGTCGTGTTGCGTTAATTTATGCCCGAAAATAAAGAAAGCACCCAAAAAAAAAAATAACGTGCGGGTTTTTTTTTTTAAAAGGAGGCGGTTTTTTTTAATAAATATTTTTAAATAAATCAGCAAAGGt                                                            >  1:810125/1‑140 (MQ=255)
                                                                                   ctTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATAGTTTTAAATTTAAGAAGCAATGGttg                                                          >  2:1434630/1‑139 (MQ=255)
                                                                                        tctcCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGaa                                                     <  1:898285/140‑1 (MQ=255)
                                                                                        tctcCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGaa                                                     <  1:367830/140‑1 (MQ=255)
                                                                                            cGGTCGTTTTACTTTGATTTATCCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAAAAAGGGGCGGATTTATTTTTTAAAAAAGAGTCGTTATATTTGTAAAATATTTTTTAATTAAATAAGCAATGGTTAGGGAatata                                                 >  2:300517/1‑139 (MQ=255)
                                                                                                    ttCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGa                                         <  2:1047325/140‑1 (MQ=255)
                                                                                                    ttCCTTTCATGTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGa                                         <  2:723452/140‑1 (MQ=255)
                                                                                                                 tcCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTg                                                                                            <  2:748616/77‑1 (MQ=255)
                                                                                                                 tcCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTg                                                                                            >  1:748616/1‑77 (MQ=255)
                                                                                                                 tcCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGc                            >  1:791452/1‑140 (MQ=255)
                                                                                                                 tcCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGc                            >  1:251284/1‑140 (MQ=255)
                                                                                                                    agTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCTGTAATATAATAAAAGACATTCTTGCTGGGAtt                         <  2:689892/139‑1 (MQ=255)
                                                                                                                      tAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCAtttt                       >  1:1507712/1‑140 (MQ=255)
                                                                                                                         aaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGACAAGACATTCTTGCTGGCATTTTAGt                    <  1:67509/140‑1 (MQ=255)
                                                                                                                              agaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt               >  2:701602/1‑140 (MQ=255)
                                                                                                                              agaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt               >  2:592673/1‑140 (MQ=255)
                                                                                                                                aaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTa             <  2:1266199/140‑1 (MQ=255)
                                                                                                                                aaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTa             <  2:685661/140‑1 (MQ=255)
                                                                                                                                 aagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAg            <  1:1459887/140‑1 (MQ=255)
                                                                                                                                 aagCTCCCAATAAAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAg            <  2:1416744/140‑1 (MQ=255)
                                                                                                                                    cTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTTCATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAt         >  1:594632/1‑140 (MQ=255)
                                                                                                                                    cTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCAGTTTAGTTGCGTTAGCAt         >  1:1494267/1‑140 (MQ=255)
                                                                                                                                     tCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAtt        <  2:462958/140‑1 (MQ=255)
                                                                                                                                     tCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAtt        <  2:1400568/140‑1 (MQ=255)
                                                                                                                                     tCCCAATACAACAAAGACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTAAATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGGTGCGTTAGCAtt        <  2:1190665/140‑1 (MQ=255)
                                                                                                                                      cccAAGACCACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTAGCGTTAGCATTa       <  1:1315270/140‑1 (MQ=255)
                                                                                                                                       ccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAATACATTCTTGCTGGGAGTTTAGTTGCGTTAGCATTaa      >  1:107444/1‑140 (MQ=255)
                                                                                                                                       ccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTaa      <  2:1676671/140‑1 (MQ=255)
                                                                                                                                          aTACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGGTAGCATTAATAc   >  1:1446860/1‑140 (MQ=255)
                                                                                                                                           tACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATAcc  <  1:107111/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
ATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACC  >  NZ_CP034943/176912‑177190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: