Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,539,5920CG26.6% 253.5 / 22.5 131E121Q (GAA→CAA) EO946_RS18215T7SS effector LXG polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base C (28/63);  new base G (33/0);  total (68/63)
Fisher's exact test for biased strand distribution p-value = 1.49e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 142 total aligned reads displayed.

CATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAT  >  NZ_CP034943/3539457‑3539730
                                                                                                                                       |                                                                                                                                          
catcaAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTtgttct                                                                                                                                        >  1:1208561/1‑140 (MQ=255)
catcaAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTtcgtct                                                                                                                                        >  2:1499396/1‑140 (MQ=255)
 atcaAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttcgtgtt                                                                                                                                       >  1:596774/1‑135 (MQ=255)
 atcaAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTgttctt                                                                                                                                       >  1:1894478/1‑140 (MQ=255)
 atcaAGCTTATCGACTGCTGTGAGTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATCGTCGGCAAGCGTGTACATGTCGAGATCAATCATCTCGCTGATGCGTGACAAAATGGAGTCGAGttcttggt                                                                                                                                       >  1:114041/1‑137 (MQ=255)
   caAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTa                                                                                                                                     >  2:736268/1‑140 (MQ=255)
   caAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTCTTCtttt                                                                                                                                     >  2:206493/1‑139 (MQ=255)
         tATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCg                                                                                                                               <  1:1701388/140‑1 (MQ=255)
           tCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGga                                                                                                                             <  2:702183/140‑1 (MQ=255)
              aCTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGAc                                                                                                                          <  2:292491/140‑1 (MQ=255)
              aCTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGAc                                                                                                                          <  2:1760319/140‑1 (MQ=255)
              aCTGCTGTGATTGTGGCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACAAGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTCTGAGCGGAGAc                                                                                                                          <  2:162181/140‑1 (MQ=255)
                tgctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACaa                                                                                                                        >  2:943229/1‑140 (MQ=255)
                tgctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACaa                                                                                                                        >  1:785349/1‑140 (MQ=255)
                 gctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACTTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTTTGAGCGGAGGCaag                                                                                                                       >  2:105412/1‑139 (MQ=255)
                  ctcttaTTGTGTCACGTCTGATTTTTTTAGCAACGCACATCTTATCGGCATAGTCGTCAAGCGTGTACATTTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTATTAGCGGAGACaaat                                                                                                                      >  2:579884/6‑138 (MQ=255)
                  ctcttaTTGTGTCACGTATGATTTTTTGGGCAACGCAAATCTTATCGGCATATTCGTCAAGCGTGTACATGTCGATATCAATAATATAGCTGATGCCTGACAAAATGGAGTCGATTTATTATTTATAATCGGAGACaaat                                                                                                                      >  2:1263100/6‑138 (MQ=255)
                      gATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTg                                                                                                                  <  1:677232/140‑1 (MQ=255)
                      gATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATAGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTg                                                                                                                  <  1:498151/140‑1 (MQ=255)
                      gATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATAGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGAAAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTg                                                                                                                  <  1:498231/140‑1 (MQ=255)
                       aTTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTTTTGGTTATGAGCGGTGACAAATTCTGa                                                                                                                 >  1:125129/1‑140 (MQ=255)
                       aTTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATTAGCGGAGACAAATTATgt                                                                                                                 >  1:124352/1‑139 (MQ=255)
                       aTTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTATTCTTTATGAGCGGAGACAATTTCTGa                                                                                                                 >  2:97909/1‑140 (MQ=255)
                        ttGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAg                                                                                                                <  1:53900/140‑1 (MQ=255)
                          gtgtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGc                                                                                                              >  2:80811/1‑140 (MQ=255)
                           tgtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTGTTTATGTTCGGAGACAATTTATGAGGCt                                                                                                             >  2:1239463/1‑140 (MQ=255)
                            gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGATCGGAGACAATTTATTAGGCtt                                                                                                            >  1:1096406/1‑140 (MQ=255)
                            gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGATCGGAGACAAATTCTGAGGCtt                                                                                                            >  2:238160/1‑140 (MQ=255)
                            gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATTAGCGGAGACAAATTATTAGGCtt                                                                                                            >  1:1790985/1‑140 (MQ=255)
                            gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATTAGCCGAGACAAATTCTGAGGCtt                                                                                                            >  2:1792013/1‑140 (MQ=255)
                            gtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATGAGCGGGGTAAATTTCTGAGGCtt                                                                                                            >  1:1490825/1‑140 (MQ=255)
                             tCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCttt                                                                                                           <  2:483668/140‑1 (MQ=255)
                               aCGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATTATCGGAGACAATTTATGAGGCtttaa                                                                                                         >  2:1605466/1‑138 (MQ=255)
                               aCGTCTGATTTTTTGGGAATCGCCCATCTTATCGGCATAGTCGTCAAGAGTGTACATGTCGAGATCAATAATATCGCTGATTCCTGACAAAATGGATTCGATTTGTTGTTTATGAGCGGAGACAATTTATGATGAtttaa                                                                                                         >  2:502470/1‑138 (MQ=255)
                                  tCTTATTTTTTGGGCATCGCCCATATTATCTGCATATTATTAAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCATGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTAAAGt                                                                                                      <  2:180909/140‑1 (MQ=255)
                                  tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  2:1381515/140‑1 (MQ=255)
                                  tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                      <  2:1232563/140‑1 (MQ=255)
                                            tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                            <  2:1778778/140‑1 (MQ=255)
                                            tGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                            <  2:1966623/140‑1 (MQ=255)
                                             gggCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGc                                                                                           <  1:354208/140‑1 (MQ=255)
                                                   cGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGATCGTTTTGAGCGGAGCCAATTTGTGAGGCTTTGAAGTGGCCCTTGGGGAGcgcg                                                                                     >  1:1296235/1‑137 (MQ=255)
                                                        aTCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATc                                                                                <  1:579884/140‑1 (MQ=255)
                                                        aTCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATc                                                                                <  1:1263100/140‑1 (MQ=255)
                                                             aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAAAATATTAGGCTTTCAAGTCTCCATTGGGCACCTCATGATCCaaaa                                                                           >  2:446191/1‑140 (MQ=255)
                                                                ggCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaag                                                                        <  2:1914875/140‑1 (MQ=255)
                                                                ggCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaag                                                                        <  1:1072431/140‑1 (MQ=255)
                                                                ggCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaag                                                                        <  2:703480/140‑1 (MQ=255)
                                                                  cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATTAGCGGTGACTATTTGTGAGGGTTTCAATTCGCCATTGGTCTGCTGATGTTCCAAAAaagaa                                                                      >  2:688085/1‑140 (MQ=255)
                                                                    tAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTATCCATTTGGCAGCTCATGATACAAAAAAGAAGt                                                                    >  2:1401805/1‑140 (MQ=255)
                                                                       tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                                 <  1:1510994/140‑1 (MQ=255)
                                                                           cAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATTATCGGGGACAAATTCTGAGGGGTTAAAGTATACAATTGGCCGCTCAAGATACAAAAAATAAATTTAcaca                                                             >  1:764000/1‑140 (MQ=255)
                                                                           cAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGGTTAGGAGGGGCGACCAATTCTGGGGCGTTGAAGTCGCCATTGGGCAGCTCATGATCCCAAAAAAGAGTTTCcaca                                                             >  1:936830/1‑140 (MQ=255)
                                                                            aaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACat                                                            <  1:1143179/140‑1 (MQ=255)
                                                                              gCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACatat                                                          <  2:797704/140‑1 (MQ=255)
                                                                              gCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACatat                                                          <  1:752930/140‑1 (MQ=255)
                                                                              gCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACatat                                                          <  2:957378/140‑1 (MQ=255)
                                                                              gCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAAGGGAGTCGATTTCTTCGTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACatat                                                          <  2:1701573/140‑1 (MQ=255)
                                                                               cGTGTACCTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATg                                                         <  1:1277447/140‑1 (MQ=255)
                                                                               cGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATg                                                         <  1:1263733/140‑1 (MQ=255)
                                                                               cGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATg                                                         <  1:1263336/140‑1 (MQ=255)
                                                                               cGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATGTGTTCTTTTTGTGCGGTGACAAATTGTGAGGCTTTGAAGTCTCCATTTGGGAGCTCATGATCCAAAAAAGAAGTTTGCACatatt                                                         >  1:981599/1‑139 (MQ=255)
                                                                               cGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATGAGCGGAGTCCATTTCTGAGGGTTTGAAGTCTCCATTTGGGAGCTCATTATACAAAAAAGAATTTTGCCCAAATg                                                         >  1:1336065/1‑140 (MQ=255)
                                                                               cGTGTACATGTCGAGATCAATAAGATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATg                                                         <  1:1168511/140‑1 (MQ=255)
                                                                               cGTGTACATGACGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATg                                                         <  1:1013642/140‑1 (MQ=255)
                                                                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATTAGCGGAGACAATTTGTGTGGCTTTCAAGTCTCCAATGGGCAGCTCATGATACAAAAAAGAAGTTTGCACAAATGaa                                                       >  1:1032179/1‑140 (MQ=255)
                                                                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                       >  2:384484/1‑140 (MQ=255)
                                                                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGGTGTTTATGAGCGGAGCCAATTTGTGAGGCGTTCCACTCTGCAGTGGGCCGCTCATGATCCACAAAAGAAGTTTCCACAGATgca                                                       >  2:1693645/1‑138 (MQ=255)
                                                                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGTGCGGAGACAATTTATGATGCTTTCAAGTCTCCATTTGGCAGCTCATGATGCAAAAAAGAAGTTTCCACATATGaa                                                       >  2:438014/1‑140 (MQ=255)
                                                                                 tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCGTTCAAGTGGCCATTGGGCAGCTCATGATGCAAAAAAGAAGTTTCCCCATATGaa                                                       >  2:1063045/1‑140 (MQ=255)
                                                                                   tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGGTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATc                                                     >  2:1008462/1‑140 (MQ=255)
                                                                                   tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGGTCGTTATGAGCGGAGACCATTTGTGAGGCGTTGAAGTGTCCCTTGGGCAGCGCCTGATCCCAAAAATAAGTGTCCCCATATGGATc                                                     >  2:110077/1‑140 (MQ=255)
                                                                                   tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTTTTCTTTTTGAGCGGCGTCAAATTCTTAGGCTTTCAAGTGGGCATTTGGGCGCGCATTATACAAAAAATAAATTTCCACAAAAGaaac                                                     >  1:1219750/1‑138 (MQ=255)
                                                                                       tGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTc                                                 <  1:1063045/140‑1 (MQ=255)
                                                                                       tGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTc                                                 <  1:384484/140‑1 (MQ=255)
                                                                                        gTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGCAAAGTTCTGAGGCTTTAAAGTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCAttga                                                >  2:744259/1‑138 (MQ=255)
                                                                                             gaTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATTATCCGAGACAAATTATTAGGCTTTGTAGTATCCATTGGGCAGCTCAATATGCAAAAAAAAATTTTGAACAAATTAATAAttaatttgt                                           >  1:311542/1‑133 (MQ=255)
                                                                                              aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                          <  2:1665898/140‑1 (MQ=255)
                                                                                               tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                         <  2:1903613/140‑1 (MQ=255)
                                                                                               tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                         <  2:1722862/140‑1 (MQ=255)
                                                                                                cAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCGCCATTGGGCAGCGCATGATCCAAAAAAGAAATTTCCACATATGAATCATTAAGTTCTTGa                                        >  2:543994/1‑140 (MQ=255)
                                                                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAAATTCTTAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTACACAAATGAATCATTAAGTTCTTGATATTTAAt                                >  2:137763/1‑140 (MQ=255)
                                                                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                <  1:238160/140‑1 (MQ=255)
                                                                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                <  1:212808/140‑1 (MQ=255)
                                                                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAAt                                <  2:125129/140‑1 (MQ=255)
                                                                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAAATTATGAGGCTTTGAAGTCTACATTGGGCAGCTCATGATCCAAAAAAGAAGTTTGCACATAAGAATCATTAAGTTCTTTTTCTTTAAt                                >  2:1456156/1‑140 (MQ=255)
                                                                                                        tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCGTTATGAGCGGAGACAATTTATGATGCTTTGAAGTCTCCATTTGGCACCTCATGATCCAAAAAAGAAATTTACAAATATTAAACATTCAGTTGTTGATATTTAAt                                >  2:521964/1‑140 (MQ=255)
                                                                                                           cTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGAGACAATTTCTGAGGGGTTCAAGTCTCCATTGGGGAGCTCATGATGCAAAAAAGAAGTTTGCACATATGAATCATTCAGTTGTTTATCTTTAATGta                             >  2:43150/1‑139 (MQ=255)
                                                                                                           cTGATGCCTGACAAAATGGAGTCGATTTGTTGGTTATTAGCGGGGTCAATTTTTGTGGGTTTGAAGTGTCCATTGGGCCGCTCATGTTGCAAAAAATAATTTTGCACATATTAATAAATGAGTTGTTGATGTTTTAtttg                             >  1:1422604/1‑137 (MQ=255)
                                                                                                           cTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGGGACAATTTCTGAGGCTTTGAAGTCTCCATTGGGCCGCTCATGATCCAAAAAAGAAGGTTCCAAATATGAATAAGTCAGTTCTTGATGTTTAATGtg                             >  2:1209753/1‑139 (MQ=255)
                                                                                                           cTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGtc                             <  1:97909/140‑1 (MQ=255)
                                                                                                           cTGATGCCTGACAAAATGGAGTCGATTTCTTCGTTATGAGCGGAGACAATTTGTGAGGGGTTCAAGTCTCCAGTGGGCAGCTCATGATACAAAAAAGAACTTTCCACAAATGTATCATTCAGTTCTTGATCTTTAATGtg                             >  2:408827/1‑139 (MQ=255)
                                                                                                                gCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTg                        <  2:1107753/140‑1 (MQ=255)
                                                                                                                        aaaTGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCa                <  2:316358/140‑1 (MQ=255)
                                                                                                                              aGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTaaa          <  1:1708479/140‑1 (MQ=255)
                                                                                                                               gTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTGAACTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAATTTCCACAAATTAATAATTCAGTTGTTTATATTTAATTTCTACTGAAAAGCCAAATaaaa         >  2:9836/1‑140 (MQ=255)
                                                                                                                                  gATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTACATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCAGATATGCATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAATCg      <  2:923041/140‑1 (MQ=255)
                                                                                                                                   aTTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTAAAGTCTCCAATGGGCAGCTAATGATCCAAAAAAGAACTTTACACATATTAATCATTAAGTTATTGATATTTAATTTATCCATAAATTCCAATTAAAAAAGc     >  2:541768/1‑140 (MQ=255)
                                                                                                                                      tcttctTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAt  <  1:616488/140‑1 (MQ=255)
                                                                                                                                      tcttctTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAt  <  1:1350287/140‑1 (MQ=255)
                                                                                                                                      tctactATATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAt  <  1:90760/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAACGCAAT  >  NZ_CP034943/3539457‑3539730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑