Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943595,1860GT23.4% 253.0 / 17.5 128L24F (TTG→TTT) EO946_RS03130MMPL family transporter
Reads supporting (aligned to +/- strand):  ref base G (34/64);  new base T (30/0);  total (64/64)
Fisher's exact test for biased strand distribution p-value = 2.10e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 137 total aligned reads displayed.

CTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACA  >  NZ_CP034943/595049‑595321
                                                                                                                                         |                                                                                                                                       
cTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAgtttgtt                                                                                                                                       >  2:1134326/1‑140 (MQ=255)
    aaTTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTtct                                                                                                                                   <  2:237327/140‑1 (MQ=255)
    aaTTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTtct                                                                                                                                   <  1:16623/140‑1 (MQ=255)
     aTTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGttttatg                                                                                                                                  >  2:1900170/1‑137 (MQ=255)
         tATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGaa                                                                                                                              <  1:1278172/140‑1 (MQ=255)
         tATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGaa                                                                                                                              <  1:493553/140‑1 (MQ=255)
              tAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTaa                                                                                                                         <  1:463756/140‑1 (MQ=255)
              tAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTaa                                                                                                                         <  2:703839/140‑1 (MQ=255)
               agagATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTGTTTTCTCCGAACTTAAc                                                                                                                        >  2:1840932/1‑140 (MQ=255)
                 agaTATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTCCGAACTTAACgg                                                                                                                      >  2:549660/1‑140 (MQ=255)
                 agaTATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACgg                                                                                                                      >  2:549131/1‑140 (MQ=255)
                   atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAg                                                                                                                    <  1:365650/140‑1 (MQ=255)
                     atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGATTTTTTTTTCTCCGAACTTAAAGGAGCt                                                                                                                  >  1:912013/1‑140 (MQ=255)
                     atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTACGAACTTAAAGGAGCt                                                                                                                  >  2:101326/1‑140 (MQ=255)
                     atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATCCGAACTTAAAGGAGCt                                                                                                                  >  2:705297/1‑140 (MQ=255)
                     atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGAACTTAAAGGAGCt                                                                                                                  >  2:200186/1‑140 (MQ=255)
                           cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTTTTTTATCCGAACTTAAAGGAGCTGGCCaa                                                                                                            >  1:329330/1‑140 (MQ=255)
                           cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCaa                                                                                                            <  2:1353991/140‑1 (MQ=255)
                             ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTTGTTTTTTTTGTCCGAAAATAAAGGATCTGGcccaaa                                                                                                          >  1:1505201/1‑136 (MQ=255)
                             ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTTTTTTATACGAACTTAACGGATCTGGCCaaaa                                                                                                          >  1:1311496/1‑138 (MQ=255)
                             ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGATTTTTTTTTCTACGAACTTAAAGGAGCTTGCCAATc                                                                                                          >  2:1661624/1‑140 (MQ=255)
                             ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTCCGAACTTAACGGAGCTGGCCAATc                                                                                                          >  2:947668/1‑140 (MQ=255)
                                     aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                  <  2:590879/140‑1 (MQ=255)
                                      gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  1:978448/140‑1 (MQ=255)
                                      ccAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                 <  1:814377/139‑1 (MQ=255)
                                          aTTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGGc                                                                                             >  1:1715870/1‑140 (MQ=255)
                                             tAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCa                                                                                          <  1:1794312/140‑1 (MQ=255)
                                             tAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCa                                                                                          <  1:1207462/140‑1 (MQ=255)
                                                 ggaggaGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCtt                                                                                      <  1:545504/140‑1 (MQ=255)
                                                     gagaTTCTGCAGAATGAGAGCACTCATCAAATTCAAGTGGGCACTTGCAGCGGTTATATTAGCACGTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTg                                                                                  <  1:1039518/140‑1 (MQ=255)
                                                     gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTCCGAAATTAACGGAGCTGGCCaa                                                                                                            >  1:1203569/1‑114 (MQ=255)
                                                     gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCaa                                                                                                            <  2:1203569/114‑1 (MQ=255)
                                                     gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTTGTTTGTTTTGTCCGAACTTAAAGGAGCTGGCCCAACAAAATGGGCAGGCCCAGCTTACTg                                                                                  >  1:1750047/1‑140 (MQ=255)
                                                     gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGATTTTTTTTTCTCCGAACATAAAGGATCAGGCCAATAAAAATGGGCAGGCCCAGCTTCCTg                                                                                  >  1:1950557/1‑140 (MQ=255)
                                                           cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:1397807/140‑1 (MQ=255)
                                                           cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:1302593/140‑1 (MQ=255)
                                                           cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:503264/140‑1 (MQ=255)
                                                           cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:228112/140‑1 (MQ=255)
                                                           cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:1906742/140‑1 (MQ=255)
                                                           cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:1472665/140‑1 (MQ=255)
                                                           cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTGACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                            <  1:1367237/140‑1 (MQ=255)
                                                            tGCAGAATGAGAGCACTCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTGTTTTCTCCGCACTTAAAGGAGCTGGCCAATCAAAAGGGGCACGCCCAGATTCCAGCAGATga                                                                           >  2:582640/1‑139 (MQ=255)
                                                            tGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCAAACAAAAGTGGCAGGCCCAGCTTCCTTCAGATGc                                                                           >  2:209360/1‑140 (MQ=255)
                                                            tGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGGTATATGAGCACTTACCGTCGTGTTTTGTTTGTTTTCTCCGAAATTAACGGAGCTGGGCCAACAAAAAGGGCCGGCCCCGCTTCCTGCAGCTGc                                                                           >  2:812556/1‑140 (MQ=255)
                                                             gCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTGTTTTGTACGGACTTAACGGAGCTGGCCCATCAAAAGGGGCCGGCCCAGCTTCCTGCCGATGCt                                                                          >  1:724108/1‑140 (MQ=255)
                                                                gAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGtt                                                                       <  2:419134/140‑1 (MQ=255)
                                                                 aaTGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTGTTTTGTCCGCACTTAACGGAGCGGGCCAAACAAAATGGGCCGGCCCAGCTTGCAGCAGATTCttgtt                                                                      >  1:1922268/1‑136 (MQ=255)
                                                                  aTGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATAAAAAGGGGCAGGCCCAGCTTCCTGCCGATGCTGttta                                                                     >  2:37820/1‑139 (MQ=255)
                                                                   tGAGAGCATTCATCCTATTCAAGTGGGCAATTGCAGCGGGGGTATTAGCACTTACCGTCGTGGTGAGTTTGTTTTCGCGGGACGTAAAGGACCTGGCGACTCAAATGGGGCCGGCGCCGCTGCCTGCCGATGCTGGTTcc                                                                    >  2:980702/1‑139 (MQ=255)
                                                                   tGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTATACGAAATTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGtttgc                                                                    >  1:1810058/1‑138 (MQ=255)
                                                                     agagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGa                                                                  <  1:812799/140‑1 (MQ=255)
                                                                      gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTGTTTTATCCGAAATTAACGGATCTGGCCAATCAAAAGGGGCAGGCCCACCTTCCTGCAGATGCTTTTTCATaa                                                                 >  2:965527/1‑140 (MQ=255)
                                                                      gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTCCGAACATAAAGGAGCATGCCCAGCAAAATGGGCAGGCCCAGCGTCCGGCAGATGCTGTTTCAGaa                                                                 >  2:1849835/1‑140 (MQ=255)
                                                                      gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTGCGAACTTAACCGATCTGGCCAAAAAAAAGGGGCAGGCCCAGCTTCCTGCAGAAGCTGtttaacaa                                                                 >  2:150053/1‑135 (MQ=255)
                                                                      gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGaa                                                                 <  2:435700/140‑1 (MQ=255)
                                                                      gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGaa                                                                 >  2:162549/1‑140 (MQ=255)
                                                                           ctcatcaCATTCAAGTGGGCAATTGCAGCGGTTATAGTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCCATCAAAAGGGGGAGGCCCCGCTTCCGGCACATGCAGTTTCAGAAAGAGc                                                            >  2:1414432/2‑140 (MQ=255)
                                                                           atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTATTTTTTTTTATGCGAAATTAACGGAGCTGGCCAAAAAAAAAGGGCAGGCCCAGCATACTGCAGCTTCTTTTTAAGAAAGAGc                                                            >  2:91504/1‑140 (MQ=255)
                                                                                 aaaTTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGc                                                      <  1:1640424/140‑1 (MQ=255)
                                                                                        aGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTTGTTTGTTTTATCCGAACTTAAAGGAGCTGGCCCAAAAAAAAGGGCACGCCCACCTTGCTGCCGATTCTGTTTGAGAAAGAGCCAAAGCCCATTTa                                               >  1:375805/1‑140 (MQ=255)
                                                                                        aGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTa                                               <  2:1040343/140‑1 (MQ=255)
                                                                                        aGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTTTTTTTTTTTTGCGAACGTAAAGGAGCTGGCCACTCAAAAGGGGCAGGCCCAGCTTGCTGCCTAAGCTGTTTACGAAAGAGCAAATGCCAATTTa                                               >  1:1229614/1‑140 (MQ=255)
                                                                                        aGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGGTTTGTTTGACCGAACTTAAACGCGCGGGCCAATCAAAAAGGGCAGGCCCCGCTTACTGCCGAAGCTTTTTCAGAAAGAACAAATTACATTTTa                                               >  1:1813223/1‑140 (MQ=255)
                                                                                           gtgaaaTTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGATTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATAAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTaaaa                                            <  2:914179/136‑1 (MQ=255)
                                                                                            ggCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTGAGCGAACTTAACAGAGCGTGCCAATCAAAAGCGGCCGGCCCACCTTCCTTCACAAGCTGTTTGAGAAAGACCAAATGCCAATTTaaaaa                                           >  1:1567238/1‑139 (MQ=255)
                                                                                            ggCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTTTTTTGTCCGAACTTAAACGAGCTGGGCCAACAAAAAGGGCAGGCCCACCGTACTGCAGATTCTTTTTCAGAAAGATCAAATTCCATTTTaaaaa                                           >  1:358641/1‑139 (MQ=255)
                                                                                                      gCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGgaga                                 <  1:909272/140‑1 (MQ=255)
                                                                                                       cGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGagaa                                <  1:318603/140‑1 (MQ=255)
                                                                                                       cGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGagaa                                <  1:67090/140‑1 (MQ=255)
                                                                                                         gTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                       <  1:984077/67‑1 (MQ=255)
                                                                                                         gTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                       <  1:1275041/67‑1 (MQ=255)
                                                                                                         gTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                       >  2:1275041/1‑67 (MQ=255)
                                                                                                         gTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGagaaga                              <  1:101326/140‑1 (MQ=255)
                                                                                                           tataTTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACa                            <  1:37820/140‑1 (MQ=255)
                                                                                                              atTAGCACTTACCGTCGTGTTGTGTTTTTTTTTTGCGAACTTAACGGAGCTGGTCCATCAAAAGGGGCCGGCCCACCTTGCTGGAGATTCTTTTTGAGAAATAGCAAAATCCATTTTTAAAAAAACGGGAGTAGACaaaa                         >  1:1019018/1‑138 (MQ=255)
                                                                                                                   cACTTACCGTCGTTTTGAGTTTTTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAAAAAGCGGGAGAAGACAATAATTCg                    >  2:1732624/1‑140 (MQ=255)
                                                                                                                   cACTTACCGTCGTTTTGAGTTTGTTTTCTGCGAACTTAACGGAGCTGGGCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAATAGCAAATGCCATTTTAAAAAAAACGGGAGAATACAATAAttag                    >  2:445374/1‑138 (MQ=255)
                                                                                                                    aCTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGa                   <  2:1133342/140‑1 (MQ=255)
                                                                                                                     cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAACAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                  <  1:985865/140‑1 (MQ=255)
                                                                                                                     cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                  <  2:1860362/140‑1 (MQ=255)
                                                                                                                     cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                  <  2:1505201/140‑1 (MQ=255)
                                                                                                                     cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                  <  2:1750047/140‑1 (MQ=255)
                                                                                                                     cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                  <  2:637030/140‑1 (MQ=255)
                                                                                                                     cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                  <  1:1267275/140‑1 (MQ=255)
                                                                                                                     cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                  <  2:1715870/140‑1 (MQ=255)
                                                                                                                      ttACCGTCGTTTTGAGTTTTTTTTATCCGAACTTTAAGGAGCTGGCCAATAAAAAGTTGCAGGGCCAGCTTGCGGCAGATTGAGTGTGAGAAAGAGCCATAGCAATTTTAAAAAGACCGGGGGAAGAGaaaaagtggctc                 >  2:1074678/1‑133 (MQ=255)
                                                                                                                       tACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCa                <  2:1220679/140‑1 (MQ=255)
                                                                                                                        aCCGTCGTTTTTTGTTTTTTTTATCCGAAATTAAAGGAGCTGGCCCATAAAAAGGGGCAGGCCCAGCTTACATCAGATGCTGTTTTAGAAAGAGAAAAAGGCATTTTAAAAAAAACGGGAGAAGACAAAAAATAGATCac               >  2:495594/1‑139 (MQ=255)
                                                                                                                        aCCGTCGTTTTTAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCACCATCCTGCAGATGCTGTTTAAGAAATAGCAAATGCCAATTTAAAAAAACCGGGAGAACACAAAAATTCGAtaaa               >  2:1387904/1‑137 (MQ=255)
                                                                                                                        aCCGTCGTGTTGAGTTTTTTTTGTCCGAACTTAACGGAGCTGGCCCAACAAAATGGGCCGGCCCAGCTTCCTGCAGATGCAGTTTCAGAAAGAGCCAAAGCCATTTTAAAACAAGCCGGAGAAGACAATAATTAGAACAg               >  2:831131/1‑140 (MQ=255)
                                                                                                                        aCCGTCGTGTTGAGTTTTTTTTGTCCGAACTTAAAGGAGCGGGCCAATCAAAAGGGGCACGCCCAGCTTCCTGGAGTTGCTGTTTGAGAAAGAGCAAATGCCAATTTAAAACAAGCGGGAGAATACAAAAATTAGATAAg               >  2:829445/1‑140 (MQ=255)
                                                                                                                        aCCGTCGTGTTGAGTTTGTTTTATCCGAACTTAACGGAGCTGGCCCATCAAAAGGGGCAGGCCCAGCTTCCGGCAGATGCTGTTTGAGACAGAGCCAATGCCATTTTAAAACAAGCGGGAGAAGACCAAAATTAGCTCAg               >  2:1573635/1‑140 (MQ=255)
                                                                                                                           gtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt            <  2:216036/140‑1 (MQ=255)
                                                                                                                            tcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc           <  1:445374/140‑1 (MQ=255)
                                                                                                                                tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt       <  1:949597/140‑1 (MQ=255)
                                                                                                                                 ttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCGTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCtt      <  1:1387904/140‑1 (MQ=255)
                                                                                                                                     ttttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTACTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa  <  1:1849835/139‑1 (MQ=255)
                                                                                                                                     gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa  <  1:200428/140‑1 (MQ=255)
                                                                                                                                     gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa  <  1:705297/140‑1 (MQ=255)
                                                                                                                                     gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCGGCAGATGCTGTTTCAGAAAGAGCAACTGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa  <  2:412586/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CTTAAATTCTATCATAGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACA  >  NZ_CP034943/595049‑595321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑