Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 704,348 Δ1 bp 100% intergenic (+170/‑14) EO946_RS03650 → / → EO946_RS03655 CamS family sex pheromone protein/phosphotransferase enzyme family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943704,3480A.99.5% 994.7 / ‑2.3 200intergenic (+170/‑14)EO946_RS03650/EO946_RS03655CamS family sex pheromone protein/phosphotransferase enzyme family protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (74/125);  total (75/125)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 233 total aligned reads displayed.

GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGAAT  >  NZ_CP034943/704209‑704485
                                                                                                                                           |                                                                                                                                         
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                           <  2:2625840/140‑1 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                           <  2:1116559/140‑1 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                           >  1:578775/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                           >  1:4367556/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                           >  2:4601219/1‑140 (MQ=255)
gCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaaga                                                                                                                                           >  2:4650788/1‑140 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                          <  1:106294/140‑1 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                          <  1:567538/140‑1 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                          <  1:4133050/140‑1 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                          <  1:2787333/140‑1 (MQ=255)
 cacaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaa                                                                                                                                          <  1:1771288/140‑1 (MQ=255)
  acaAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                         <  1:4404281/140‑2 (MQ=255)
      gggTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAg                                                                                                                                    >  2:774798/1‑140 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                               <  2:4367556/140‑1 (MQ=255)
           tttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGa                                                                                                                               <  1:1616305/140‑1 (MQ=255)
            ttttATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGAc                                                                                                                              <  2:10432/140‑1 (MQ=255)
                 tGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                         <  2:3989712/125‑2 (MQ=255)
                 tGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                         >  1:2770704/1‑124 (MQ=255)
                 tGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCaaagaag                                                                                                                                         >  1:3989712/1‑124 (MQ=255)
                 tGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCa                                                                                                                         >  1:1872076/1‑140 (MQ=255)
                  gTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCAt                                                                                                                        >  2:2450452/1‑140 (MQ=255)
                  gTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCAt                                                                                                                        >  2:1917544/1‑140 (MQ=255)
                  gTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCAt                                                                                                                        >  2:3544582/1‑140 (MQ=255)
                  gTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAACAGCAAAGAAGGGAGTTAGACGTGCAt                                                                                                                        >  2:197230/1‑140 (MQ=255)
                               ggAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGCACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCaaaa                                                                                                           >  1:4899562/1‑140 (MQ=255)
                                     ggAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACatat                                                                                                     >  2:3085833/1‑140 (MQ=255)
                                                tATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCATTATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGAt                                                                                          <  1:4268364/140‑1 (MQ=255)
                                                      tGCTTCTGCCGAGACACAAATCAGGGCGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGtt                                                                                    <  1:314532/140‑1 (MQ=255)
                                                          tCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTgg                                                                                >  1:2464380/1‑140 (MQ=255)
                                                          tCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTgg                                                                                >  1:2951401/1‑140 (MQ=255)
                                                          tCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTgg                                                                                >  1:3911243/1‑140 (MQ=255)
                                                            tGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCa                                                                              >  2:4590980/1‑140 (MQ=255)
                                                                   acacAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTg                                                                       <  1:2678847/140‑1 (MQ=255)
                                                                   acacAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAAGAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTg                                                                       <  2:215345/140‑1 (MQ=255)
                                                                     acaATTCAGGGTGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGAAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                     <  2:619173/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                     <  1:3693735/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                     <  1:2056457/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                     <  1:1370168/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                     <  1:3085833/140‑1 (MQ=255)
                                                                     acaAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAAGAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCg                                                                     <  1:2239651/140‑1 (MQ=255)
                                                                        aaTCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGAAGTGAATAAATAAATCAAAAACATATTTCATGAAGATCAGGTTTTGTCAGAGGCTGCGATg                                                                  >  2:2676829/1‑140 (MQ=255)
                                                                           cAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGaaa                                                               <  1:4499224/140‑1 (MQ=255)
                                                                           cAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGaaa                                                               <  1:2972458/140‑1 (MQ=255)
                                                                                 gAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGt                                                         >  1:111550/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                       >  2:531191/1‑140 (MQ=255)
                                                                                   ggTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGttt                                                       <  2:633425/140‑1 (MQ=255)
                                                                                         ttGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATACCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCaaa                                                 <  1:1589787/140‑1 (MQ=255)
                                                                                            aTGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAg                                              >  2:3617351/1‑140 (MQ=255)
                                                                                            aTGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAg                                              >  2:1747316/1‑140 (MQ=255)
                                                                                            aTGCTGGAACGCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGACGGGAGTTAGACGTGCATAACGAAATCAAAAACATAGTTCATGACGAGCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAg                                              >  2:1223443/1‑140 (MQ=255)
                                                                                               cTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCa                                           >  2:3370834/1‑140 (MQ=255)
                                                                                               cTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCa                                           >  1:1684074/1‑140 (MQ=255)
                                                                                                        gtgtGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGttt                                  >  2:2273412/1‑140 (MQ=255)
                                                                                                        gtgtGAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGttt                                  >  2:4913660/1‑140 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAACACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGtttt                                 <  2:4405374/140‑1 (MQ=255)
                                                                                                         tgtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGtttt                                 >  2:1525208/1‑140 (MQ=255)
                                                                                                          gtgAAAACAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTc                                >  1:3623729/1‑140 (MQ=255)
                                                                                                                aCAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                          <  1:3211434/140‑1 (MQ=255)
                                                                                                                aCAATCAATATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTg                          <  1:2368513/140‑1 (MQ=255)
                                                                                                                  aatcaatATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGAt                        <  2:225566/140‑1 (MQ=255)
                                                                                                                  aatcaatATCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGATTTGCCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGAt                        >  2:3903861/1‑140 (MQ=255)
                                                                                                                       atatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGa                   <  2:313314/140‑1 (MQ=255)
                                                                                                                       atatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGa                   <  2:2794127/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATATCAAAGTAGTGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGATTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                  <  1:4713112/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                  <  2:3310618/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                  <  1:1993042/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                  <  2:4859839/140‑1 (MQ=255)
                                                                                                                        tatCAAATCAATACCAAAGAAGGGAGTTAGACGTGAATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaa                  <  2:1198063/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTTGCTGATGCGGaaaa                <  2:4112796/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa                <  1:1525208/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa                <  1:1884256/140‑1 (MQ=255)
                                                                                                                          tcaaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa                <  1:4639065/140‑1 (MQ=255)
                                                                                                                          acaaatcacTAGCACAGAAGGGAGTTAGCCGTGCATAAAGAAATCAAAAACAGATTGCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGaaaa                <  2:1699145/139‑1 (MQ=255)
                                                                                                                           caaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCTTTTTCTGGCTGATGCGGAAAAc               >  2:1922189/1‑140 (MQ=255)
                                                                                                                            aaatcaaTAGCACAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATATCATCAATATCAGGTTTTGGCAGAGGCTGAGATGAAATAAGTTTTATCAAACAGCCAGGTGCGTTTTCTGGCTGATGAGGTAAACt              >  2:1637635/1‑140 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAACAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt              <  2:2851675/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGATGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt              >  2:1811207/1‑140 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAGATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt              <  2:1873362/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt              <  2:2030982/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt              <  2:475428/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt              <  2:4255212/140‑1 (MQ=255)
                                                                                                                            aaatcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACt              <  2:3866994/140‑1 (MQ=255)
                                                                                                                            aaataaatAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTTGCTGATGCGGAAAACt              <  1:3411405/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt            >  1:1205437/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt            <  2:1005914/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt            >  1:610032/1‑140 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt            <  2:760117/140‑1 (MQ=255)
                                                                                                                              atcaaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTAt            >  1:197618/1‑140 (MQ=255)
                                                                                                                                 aaTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGta         <  1:4601219/140‑1 (MQ=255)
                                                                                                                                  aTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGtat        >  2:4433464/1‑140 (MQ=255)
                                                                                                                                  aTAGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGtat        >  1:1561078/1‑140 (MQ=255)
                                                                                                                                    aGCAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGACAACTATGTatat      >  2:2364239/1‑140 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATGATGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa    <  2:881417/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCTGAAAACTATGTATATGa    <  1:1020857/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa    <  1:329153/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa    <  2:3440461/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa    <  2:1512211/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa    <  1:3981732/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa    <  1:863100/140‑1 (MQ=255)
                                                                                                                                      cAAAGAAGGGAGTTAGACGTGCATAAAGAAATCAAAAAAATATTTCATGAAGATCAGGTTTTGGGAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGa    <  2:4088351/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GCACAAGGGTTTTTTTATGTTACAATTAACAGGAATAGGAAAAAGATGTATTTTTGCTTCTGCCGAGACACAAATCAGGGGGAGGTATGTTGATGCTGGAACTCGTGTGAAAACAATCAATATCAAATCAATAGCAAAGAAAGGGAGTTAGACGTGCATAAAGAAATCAAAAACATATTTCATGAAGATCAGGTTTTGGCAGAGGCTGCGATGAAATACGGTTTCTCAAACAGCCAGGTGCGTTTTCTGGCTGATGCGGAAAACTATGTATATGAAT  >  NZ_CP034943/704209‑704485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: