BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	52,254	T→C	5.7%	intergenic (+15/‑196)	prtG → / → EO946_RS00280	sporulation‑specific protease PrtG/Veg family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	52,254	0	T	C	5.7%	830.8 / 26.2	246	intergenic (+15/‑196)	prtG/EO946_RS00280	sporulation‑specific protease PrtG/Veg family protein
Reads supporting (aligned to +/- strand): ref base T (149/82); new base C (7/7); total (157/89)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 251 total aligned reads displayed.

CGCCGTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAA  >  NZ_CP034943/52115‑52392

cGCCGTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTCTTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGt                                                                                                                                            >  2:2086270/1‑140 (MQ=255)

 gCCGTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGta                                                                                                                                           <  1:722459/140‑1 (MQ=255)

    gTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAg                                                                                                                                        <  1:3554651/140‑1 (MQ=255)

    gTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAg                                                                                                                                        <  1:2011403/140‑1 (MQ=255)

    gTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAg                                                                                                                                        <  1:2406999/140‑1 (MQ=255)

    gTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAg                                                                                                                                        <  1:2205606/140‑1 (MQ=255)

     tttATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGCGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAgg                                                                                                                                       >  2:488500/1‑140 (MQ=255)

     tttATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAgg                                                                                                                                       >  1:1225723/1‑140 (MQ=255)

     tttATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAgg                                                                                                                                       >  2:846357/1‑140 (MQ=255)

     tttATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAgg                                                                                                                                       >  2:813162/1‑140 (MQ=255)

     tttATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAgg                                                                                                                                       >  2:3131398/1‑140 (MQ=255)

     tttATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAgg                                                                                                                                       >  2:2726888/1‑140 (MQ=255)

      ttATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGga                                                                                                                                      <  1:1082318/140‑1 (MQ=255)

       tATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGgag                                                                                                                                     <  2:3927058/140‑1 (MQ=255)

       tATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGgag                                                                                                                                     <  2:1260900/140‑1 (MQ=255)

         tGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGAt                                                                                                                                   <  2:4036638/140‑1 (MQ=255)

          ggAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATc                                                                                                                                  <  2:423344/140‑1 (MQ=255)

             aCGAATCAATGTATTGGAAGTGCTCCCAAATACGGTGACACGAGAGAAAGGGCTTGGAGGTATTGAAACAATAGGAGTGGTGAGAATTGGAATGCCTTATAAGTCGAATTAACAGTTGAAAACCTGTATAGGAGATCTAt                                                                                                                               >  1:1657622/1‑140 (MQ=255)

             aCGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTAt                                                                                                                               >  1:4613604/1‑140 (MQ=255)

               gAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAATGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGc                                                                                                                             >  2:1207448/1‑140 (MQ=255)

                 aTCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCgg                                                                                                                           >  2:2310618/1‑140 (MQ=255)

                      tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                      <  2:563385/140‑1 (MQ=255)

                      tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATCGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                      <  2:4886009/140‑1 (MQ=255)

                          tGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttat                                                                                                                  >  2:3974798/1‑140 (MQ=255)

                           gggAAGTGCTCCGTAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                 >  2:2980197/1‑140 (MQ=255)

                           gggAAGTGCTCCGTAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                 >  2:3804338/1‑140 (MQ=255)

                           gggAAGTGCTCCGTAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                 >  2:2151898/1‑140 (MQ=255)

                           gggAAGTGCTCCGTAATACACTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                 >  2:141025/1‑140 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATTCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                 >  1:4034813/1‑140 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                 <  2:1783021/140‑1 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                 >  2:1900840/1‑140 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                 <  2:4189716/140‑1 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                 >  2:805602/1‑140 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                 <  1:4902639/140‑1 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGAATAGGAGATCTATGCGGGTTttttatt                                                                                                                 >  2:1999323/1‑140 (MQ=255)

                           gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACATGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                 >  2:3871986/1‑140 (MQ=255)

                                gTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACa                                                                                                            >  1:4295820/1‑140 (MQ=255)

                                gTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTGTTATTTTACa                                                                                                            >  1:516562/1‑140 (MQ=255)

                                  gCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATa                                                                                                          >  1:4067583/1‑140 (MQ=255)

                                   cTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATaa                                                                                                         >  2:1367437/1‑140 (MQ=255)

                                        aaaTACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATa                                                                                                    >  1:4980590/1‑140 (MQ=255)

                                        aaaTACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATa                                                                                                    >  2:1152799/1‑140 (MQ=255)

                                        aaaTACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATa                                                                                                    >  1:1538405/1‑140 (MQ=255)

                                        aaaTACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATa                                                                                                    <  2:911016/140‑1 (MQ=255)

                                             cGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATaa                                                                                               >  2:2620615/1‑140 (MQ=255)

                                              gCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAAt                                                                                              >  1:2968783/1‑140 (MQ=255)

                                                  aCAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGCGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAc                                                                                          >  2:2437235/1‑140 (MQ=255)

                                                  aCAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAc                                                                                          >  2:1609940/1‑140 (MQ=255)

                                                  aCAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAc                                                                                          >  2:2554503/1‑140 (MQ=255)

                                                           aaaGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTg                                                                                 >  2:1791710/1‑140 (MQ=255)

                                                           aaaGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTg                                                                                 >  1:3157684/1‑140 (MQ=255)

                                                            aaGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGc                                                                                >  1:2852741/1‑140 (MQ=255)

                                                                gCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGaa                                                                            >  1:975457/1‑140 (MQ=255)

                                                                          aTTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAg                                                                  >  2:4450823/1‑140 (MQ=255)

                                                                          aTTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAg                                                                  >  2:4092645/1‑140 (MQ=255)

                                                                           ttGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAgg                                                                 >  1:279641/1‑140 (MQ=255)

                                                                           ttGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAgg                                                                 <  1:3966224/140‑1 (MQ=255)

                                                                           ttGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAgg                                                                 <  2:3651153/140‑1 (MQ=255)

                                                                                   agagGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAg                                                         >  1:1855026/1‑140 (MQ=255)

                                                                                          tGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTg                                                  >  2:3391530/1‑140 (MQ=255)

                                                                                            cTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTc                                                >  1:2193617/1‑140 (MQ=255)

                                                                                            cTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTc                                                >  1:1979164/1‑140 (MQ=255)

                                                                                            cTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAACGAAACTTGCGGAAAATAATTGAGGAATCATAGACTTTTGTc                                                >  2:3185964/1‑140 (MQ=255)

                                                                                                gaATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCaaaa                                            <  1:3236556/140‑1 (MQ=255)

                                                                                                gaATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCaaaa                                            <  1:1838586/140‑1 (MQ=255)

                                                                                                  aTTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAaata                                          <  2:2371407/140‑1 (MQ=255)

                                                                                                    tGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAaataat                                        >  1:3655175/1‑140 (MQ=255)

                                                                                                    tGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAaataat                                        >  1:554802/1‑140 (MQ=255)

                                                                                                    tGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAaataat                                        >  1:2483487/1‑140 (MQ=255)

                                                                                                    tGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTAGGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAATTCATTGAGGACTCATAGAAGTTTGTCAAaataat                                        >  1:2867113/1‑140 (MQ=255)

                                                                                                         tGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTAt                                   >  1:770970/1‑140 (MQ=255)

                                                                                                           ccTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGACAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTg                                 >  1:3377403/1‑140 (MQ=255)

                                                                                                           ccTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGa                                                                <  2:736398/109‑1 (MQ=255)

                                                                                                           ccTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTg                                 >  1:591783/1‑140 (MQ=255)

                                                                                                           ccTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTg                                 >  2:4395959/1‑140 (MQ=255)

                                                                                                             ttATAAGTCTAATTAACAGTTGAACACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGAc                               <  2:4730045/140‑1 (MQ=255)

                                                                                                             ttATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGAc                               <  1:1791710/140‑1 (MQ=255)

                                                                                                              tataAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACa                              <  1:2090772/140‑1 (MQ=255)

                                                                                                                taAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAAc                            <  2:3433357/140‑1 (MQ=255)

                                                                                                                  aGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGt                          <  2:278428/140‑1 (MQ=255)

                                                                                                                  aGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGt                          <  2:4295820/140‑1 (MQ=255)

                                                                                                                  aGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGt                          <  1:4450823/140‑1 (MQ=255)

                                                                                                                    tCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATTATTTTATTGACAACGTCt                        <  1:2580758/140‑1 (MQ=255)

                                                                                                                     cTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATATAATTTTGTCAAAATAATTTTATTGACAACGTCtt                       >  1:1124349/1‑140 (MQ=255)

                                                                                                                     cTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAAtt                                                     <  1:3062833/110‑1 (MQ=255)

                                                                                                                      tAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCtta                      >  2:77698/1‑140 (MQ=255)

                                                                                                                        aTTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                    >  2:3614090/1‑140 (MQ=255)

                                                                                                                        aTTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                    >  1:2693422/1‑140 (MQ=255)

                                                                                                                        aTTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAACATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                    <  1:4572994/140‑1 (MQ=255)

                                                                                                                        aTTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAACATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                    <  1:3804338/140‑1 (MQ=255)

                                                                                                                        aTTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAACATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                    <  1:141025/140‑1 (MQ=255)

                                                                                                                              aGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGtt              >  1:849260/1‑140 (MQ=255)

                                                                                                                              aGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGtt              >  1:4747795/1‑140 (MQ=255)

                                                                                                                              aGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGtt              >  1:437453/1‑140 (MQ=255)

                                                                                                                              aGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGtt              >  1:1602660/1‑140 (MQ=255)

                                                                                                                              aGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGtt              >  1:1420626/1‑140 (MQ=255)

                                                                                                                                          gTATAGTAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATATTATTGACAACGTCTTATTAACGTTGATCTAATTTaa  >  1:890212/1‑140 (MQ=255)

                                                                                                                                          gTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTaa  >  1:3726060/1‑140 (MQ=255)

                                                                                                                                          gTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTaa  >  1:2101865/1‑140 (MQ=255)

                                                                                                                                          gTATAGGAGATCTATGCGGGTTTTTTATGTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTaa  >  1:1205350/1‑140 (MQ=255)

CGCCGTTTATGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAA  >  NZ_CP034943/52115‑52392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`