BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,673	A→G	5.8%	P172P (CCA→CCG) ‡	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,673	0	A	G	5.8%	491.6 / 17.1	191	P172P (CCA→CCG) ‡	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (93/81); new base G (9/2); total (102/89)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 192 total aligned reads displayed.

CTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTA  >  NZ_CP034943/607537‑607809

  acgggggAACCGGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                     <  2:2909551/137‑1 (MQ=255)

  aCCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                     <  2:608775/140‑1 (MQ=255)

  aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                     >  1:561683/1‑140 (MQ=255)

  aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCAACCGGGGAAGACGGAACagcagc                                                                                                                                     >  1:2528333/1‑135 (MQ=17)

  aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACAGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACGGGGGCAGCAGCACCGCCCgg                                                                                                                                     >  2:1362121/1‑140 (MQ=14)

   ccGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                                                    <  1:103556/140‑1 (MQ=255)

   ccGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                                                    <  1:2715874/140‑1 (MQ=255)

        gacgCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               <  1:2677074/137‑1 (MQ=255)

        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAc                                                                                                                               >  1:508697/1‑140 (MQ=255)

        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               <  2:1807349/140‑1 (MQ=255)

        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               <  1:978053/140‑1 (MQ=255)

             cGGACCAGCCGGGGCCACGGGAGCGCCAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGAGCGGTGAAGCGGGCGCACCCGGAGCGAGCGGAg                                                                                                                          >  2:195986/1‑140 (MQ=17)

             cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCGACCGGGGACACCGGACCACCCGGAGCGCCGGGAg                                                                                                                          >  2:2160360/1‑140 (MQ=17)

             cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                          >  2:2136813/1‑140 (MQ=255)

                aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCAACAGGGGAAGCGGGAGCAACCGGAGCAGCAGGAGcga                                                                                                                       >  1:3695257/1‑138 (MQ=17)

                     ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCAGGAACACCCGGAGCCACAGGATCTACAggt                                                                                                                  >  2:1883731/1‑139 (MQ=17)

                     ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCggg                                                                                                                  >  2:3681864/1‑140 (MQ=255)

                     ccGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                  <  1:4422496/140‑1 (MQ=255)

                         ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCggcgccg                                                                                                              >  2:97875/1‑135 (MQ=255)

                          gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                          >  2:2600262/1‑127 (MQ=255)

                              cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                         >  1:453408/1‑140 (MQ=255)

                               gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCATCCCGAGCGACCGGAGCAACCGGGCAAGcagtac                                                                                                        >  1:1097021/1‑135 (MQ=14)

                                    ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               <  2:2550065/112‑1 (MQ=40)

                                     cacaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                                  >  2:651091/1‑140 (MQ=255)

                                            gCTACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCCGCCGGCGCGACAGGAGCTACGGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                           <  2:3170491/140‑1 (MQ=255)

                                             cTACGGGGGAAGCCGCACCTCCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                          <  2:4072998/140‑1 (MQ=11)

                                              tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCGCAGGAACAGCCGGAGCgcgct                                                                                         >  2:1521112/1‑136 (MQ=14)

                                               aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACAGGGGAAGCCGGACCACCCGGAGCGCCAgg                                                                                        >  2:4584681/1‑140 (MQ=255)

                                               aCTGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGACGCCGGCCCAGCCGGAGCGACAgg                                                                                        >  1:1137325/1‑140 (MQ=255)

                                               aCTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGACGGAGCGACAgg                                                                                        >  1:3092658/1‑140 (MQ=255)

                                                    ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCACCCGGACCAGCCGCAGCGCCAGGAGcga                                                                                   >  1:1350998/1‑138 (MQ=14)

                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCCGCCGGAGCCACCGGAGCTACggg                                                                               >  2:2779377/1‑140 (MQ=14)

                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                                                               >  2:1421724/1‑140 (MQ=255)

                                                         ccGGACCACCCGGCGCCCCAGGAGCAGCCGGAGCCCCCGGAGCACCCGGAGCGACCGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                              <  2:4852987/140‑1 (MQ=14)

                                                             cccACCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACACGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:1085882/139‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:2156469/140‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAg                                                                          >  2:2236103/1‑140 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCGCCCGGGGATCCCGGAGACGCCGGAGCGACCGGAGCTACCGGGGAAg                                                                          >  2:3252939/1‑140 (MQ=14)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:2576044/140‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          >  2:2384181/1‑140 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:4076043/140‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:2421870/140‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:4842756/140‑1 (MQ=255)

                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                   <  1:2916657/110‑1 (MQ=40)

                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                        <  2:115386/121‑1 (MQ=40)

                                                                        cGACAGGCGCTACCGGGGACGCCGGACCACCCGGCGCGACTGGAGCGACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                               <  1:806421/140‑1 (MQ=14)

                                                                           cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGa                                                            >  1:2874534/1‑140 (MQ=255)

                                                                                gCTCCCGGGGAAGCCGGAGCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                       <  1:2392482/140‑1 (MQ=255)

                                                                                gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTACGCGGGACCACCCGGAGCGAc                                                       >  2:1917899/1‑140 (MQ=11)

                                                                                gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCAGGCCCAGCCGGAGCGACAGGAGCAACGGCGGAAACAGGCACAGCCGCAGCGAc                                                       >  2:1550876/1‑140 (MQ=11)

                                                                                cccaCCGGCGCAGCCGGACCATCCGGAGCCCCAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                       <  1:4191091/137‑1 (MQ=14)

                                                                                    ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa                                                   >  2:3293911/1‑140 (MQ=255)

                                                                                       cgccaGCCGGACCCCCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                <  2:3451431/136‑1 (MQ=11)

                                                                                         gacgCCGGACCAGCCGGTGCGACTGGCGCTGGGGGGGTAGCGGGCCCAACCGGACCGACAGGCGCTCCCGGCCAAGCGGGAGCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGCGCGACGGGAGCcac                                              <  1:4576608/137‑1 (MQ=2)

                                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                              >  1:2142236/1‑140 (MQ=255)

                                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                              <  1:902748/140‑1 (MQ=255)

                                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGACGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcag                                              >  2:4655481/1‑139 (MQ=255)

                                                                                          acgCCGGACCAGCCGGCGCGACAGGAGCTAGGGGGTAAGCCGGACCCACCGGTGCGAGAGGTGCTACCGGGGAAGCCGGCGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                             <  1:1869061/138‑1 (MQ=14)

                                                                                          aaGCCGGACCAGCCGGAGCGACAGGAGCTTCCGTGGACGCCTGACCAACAGGAGCGACAGGAGCTACCGGGGATGCCGGACCATCGGGATCGACAGGAGCTCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                             <  1:4260305/140‑1 (MQ=14)

                                                                                           aGCCGGACCTGCCGGAGCTACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGATCAACCGGAGCGACGGGAGCCACAg                                            <  1:4355876/140‑1 (MQ=255)

                                                                                           aGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                            <  1:2983509/140‑1 (MQ=255)

                                                                                           aGCCGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCCACAGGAGCAACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                            <  1:394001/140‑1 (MQ=14)

                                                                                            gagggACGAGCCGGAGCCTCAGGAGCTCGCGGGCACGCAGGCCCGTGCTGAGCCCCAGGAGGTACCGGGGTACGGGGAGCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGTCCACCCGGAGCGACGGGAGCCACAgg                                           <  2:3688887/137‑1 (MQ=255)

                                                                                            gCCGGACCACCCGGAGCCCCAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                           <  2:408639/140‑1 (MQ=255)

                                                                                            cccGGACCAGCCGGAGCGACAGGAGGTTCCTGCGCACCCGGACCTACCGGAGACACAGGAGCTACCGGGGAAGCCGGAGCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                           <  1:4240407/139‑1 (MQ=14)

                                                                                            cccGGACCAGCCGGACCGCCTGGAGCTAACGGGGAAGCCGGAACATCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                           <  1:1038704/139‑1 (MQ=14)

                                                                                             ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCCGCCGGCGCGACAGGAGCGACCGGGGGTGGCGGACCCAGCGGCGCGGCGGGGGCCACAggg                                          >  2:762642/1‑140 (MQ=14)

                                                                                              cGGACCCCCCGGACCGACAGGCGCCACCGGGGAAGCCCGACCACCCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                         <  2:3092658/140‑1 (MQ=14)

                                                                                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                        >  1:599248/1‑93 (MQ=37)

                                                                                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                         <  1:1176858/140‑1 (MQ=255)

                                                                                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                         <  1:3293911/140‑1 (MQ=255)

                                                                                                 aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                           >  1:280002/1‑87 (MQ=38)

                                                                                                 aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                           <  2:280002/87‑1 (MQ=38)

                                                                                                  ccAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTAc                                     <  1:3126558/140‑1 (MQ=255)

                                                                                                      ccGGCGCGACAGGACCTACCGGCGCAGCCGGACCCCCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                 <  2:4783464/140‑1 (MQ=17)

                                                                                                      ccGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                 <  1:4822867/140‑1 (MQ=255)

                                                                                                          cgCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                             <  1:4655597/139‑1 (MQ=255)

                                                                                                          aGCGACAGGCGCTCCCGGGGACGCCGGACCAACCGGAGCCACAGGCGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                             <  1:3688209/140‑1 (MQ=17)

                                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                             <  1:1702082/140‑1 (MQ=255)

                                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCCCAGGACCAACAGCAGCGCCGGGAGCCACAGCCGCGACTgtatacg                             >  2:3792476/1‑134 (MQ=14)

                                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCACCTGGGGACGTCGGa                        >  2:525730/1‑140 (MQ=255)

                                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTTCTGGGGACGTCGGa                        >  2:2956085/1‑140 (MQ=255)

                                                                                                                aGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                       <  1:1629848/140‑1 (MQ=255)

                                                                                                                aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGACGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                       >  1:2685864/1‑140 (MQ=255)

                                                                                                                aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                       >  2:3608905/1‑140 (MQ=255)

                                                                                                                    gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGAGCGGAGCAACCGGGGCGGCCGGACCAACCGGACCGGCGGGAGCGACCGGGGCGTGGGGGGACGTCGGagcagc                   >  2:4519656/1‑140 (MQ=14)

                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCgg                >  2:705977/1‑140 (MQ=255)

                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGAc          >  1:402315/1‑140 (MQ=255)

                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGAc          >  1:3458977/1‑140 (MQ=255)

                                                                                                                             ccaaccGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGAc          <  2:1882460/136‑1 (MQ=255)

                                                                                                                                gCCGGACCAACCGGAGCGACAGGAGCAACCGGCGCCCCCGGCCCAGGCGCAGCGTCCGCAGCGACGGGGGAAGCAGTACCAGCCCACGCGACTGGTGCCACAGGGGCTAGAGGGCAAGTCGGACCAGCCGGAGCGACggg       >  1:1180121/1‑140 (MQ=255)

                                                                                                                                cccgccccAACCCGAGCGACAGCAGCTACAGGGGCACCCGGACGATCCCGAGCGACAGGTGCTACCGGCGAAGCCGGACCATCACGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       <  2:4033404/134‑1 (MQ=17)

                                                                                                                                ccccgcccAACCGGCGCGCCAGCAGCCACCGGCGAAGCCGGACCAGCCGGAGCGCCCGGAGCTCCCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       <  2:4087676/134‑1 (MQ=18)

                                                                                                                                accGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGCGCCACAGGGGCTACTGGGGCCGTCGGACCAGCGGGAGCGACAgg       >  1:2209703/2‑140 (MQ=14)

                                                                                                                                accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       >  1:3867998/2‑140 (MQ=14)

                                                                                                                                accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       >  1:3381798/2‑140 (MQ=14)

                                                                                                                                accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       >  1:4898753/2‑140 (MQ=14)

                                                                                                                                 ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                       <  1:1300716/59‑1 (MQ=35)

                                                                                                                                  cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg     <  1:4702077/140‑1 (MQ=255)

                                                                                                                                     aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCtc  >  1:70158/1‑139 (MQ=255)

CTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTA  >  NZ_CP034943/607537‑607809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`