BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,674	A→C	6.8%	T173P (ACC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,674	0	A	C	6.8%	392.4 / 2.0	191	T173P (ACC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (84/79); new base C (12/8); total (102/89)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

CTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTA  >  NZ_CP034943/607537‑607809

cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACGGGGGAAGCCGGACCAGCCGGCGCGACAGGCGCTACCGGGGACGCCGGACCAGCCGGCGCGCCAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                                                       >  1:3451431/1‑140 (MQ=18)

  acgggggAACCGGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                     <  2:2909551/137‑1 (MQ=255)

  aCCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                     <  2:608775/140‑1 (MQ=255)

  aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                     >  1:561683/1‑140 (MQ=255)

  aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACAGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACGGGGGCAGCAGCACCGCCCgg                                                                                                                                     >  2:1362121/1‑140 (MQ=14)

   ccGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCACCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGGGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                                                    <  1:4456056/140‑1 (MQ=18)

   ccGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                                                    <  1:103556/140‑1 (MQ=255)

        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGGGGGAGCgcc                                                                                                                               >  1:270743/1‑138 (MQ=17)

        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAc                                                                                                                               >  1:508697/1‑140 (MQ=255)

        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               <  2:1807349/140‑1 (MQ=255)

        gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               <  2:2011112/140‑1 (MQ=255)

             cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCGACCGGGGACACCGGACCACCCGGAGCGCCGGGAg                                                                                                                          >  2:2160360/1‑140 (MQ=17)

                aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAACAGGCCCAGGCGCACCGGCAGGAGcga                                                                                                                       >  1:3694836/1‑138 (MQ=14)

                   cgCCGGGGCCACGGGAGCCCCCGGGGCTACTGGGCAAGCCGGCCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAcag                                                                                                                    <  1:3569450/139‑3 (MQ=11)

                     ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCggg                                                                                                                  >  2:3681864/1‑140 (MQ=255)

                     ccGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                  <  1:3839947/140‑1 (MQ=255)

                         ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCggcgccg                                                                                                              >  2:97875/1‑135 (MQ=255)

                          gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                          <  1:2600262/127‑1 (MQ=255)

                          gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                          >  2:2600262/1‑127 (MQ=255)

                               gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCggcc                                                                                                        >  1:312025/1‑138 (MQ=255)

                               gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCATCCCGAGCGACCGGAGCAACCGGGCAAGcagtac                                                                                                        >  1:1097021/1‑135 (MQ=14)

                                    ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               >  1:2550065/1‑112 (MQ=40)

                                    ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                               <  2:2550065/112‑1 (MQ=40)

                                     cacaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                                  >  2:651091/1‑140 (MQ=255)

                                             cTACGGGGGAAGCCGCACCTCCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                          <  2:4072998/140‑1 (MQ=11)

                                              tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAg                                                                                         >  1:4620104/1‑140 (MQ=255)

                                              tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCGCAGGAACAGCCGGAGCgcgct                                                                                         >  2:1521112/1‑136 (MQ=14)

                                               aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACAGGGGAAGCCGGACCACCCGGAGCGCCAgg                                                                                        >  2:4584681/1‑140 (MQ=255)

                                               aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                        >  1:951296/1‑140 (MQ=255)

                                               aCTGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGACGCCGGCCCAGCCGGAGCGACAgg                                                                                        >  1:1137325/1‑140 (MQ=255)

                                                    ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCACCCGGACCAGCCGCAGCGCCAGGAGcga                                                                                   >  1:1350998/1‑138 (MQ=14)

                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCAGCGCAGCCGGCCCAGCCGGAGCGACCGGAGCtcacgg                                                                               >  1:282252/1‑135 (MQ=11)

                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCCGCCGGAGCCACCGGAGCTACggg                                                                               >  2:2779377/1‑140 (MQ=14)

                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                                                               >  2:1421724/1‑140 (MQ=255)

                                                         ccGGACCACCCGGCGCCCCAGGAGCAGCCGGAGCCCCCGGAGCACCCGGAGCGACCGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                              <  2:4852987/140‑1 (MQ=14)

                                                             cccACCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACACGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:1085882/139‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGATCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          >  1:759057/1‑140 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:2156469/140‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAg                                                                          >  2:2236103/1‑140 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCGCCCGGGGATCCCGGAGACGCCGGAGCGACCGGAGCTACCGGGGAAg                                                                          >  2:3252939/1‑140 (MQ=14)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:4076043/140‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          >  2:2384181/1‑140 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGGCGGAGCGCCAGGAGCGACCGGCGAGGCCGGAGCAGCCGGAGCTACAGGAGCCACCggagccg                                                                          >  2:1927366/1‑135 (MQ=11)

                                                             aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:2421870/140‑1 (MQ=255)

                                                             aCCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  1:4842756/140‑1 (MQ=255)

                                                              cccgCCGGAGCGACCCGAGCGACGGGCGATGCCGGCCCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGc                                                                         <  2:4124175/137‑1 (MQ=14)

                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                   <  1:2916657/110‑1 (MQ=40)

                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                        <  2:115386/121‑1 (MQ=40)

                                                                       gCGACAGGCGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                <  1:4575096/140‑1 (MQ=255)

                                                                           cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGa                                                            >  1:4250824/1‑140 (MQ=255)

                                                                           cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGa                                                            >  1:2874534/1‑140 (MQ=255)

                                                                                gCTCCCGGGGAAGCCGGAGCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                       <  1:2392482/140‑1 (MQ=255)

                                                                                gCTCCCGGGGAAGCCGGACCCGCCGGAGGGACAGGCGCTCCGGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                       <  1:609052/140‑1 (MQ=11)

                                                                                gCTCCCGGCGAAGCCGGACCCGCCGGCGCGCCAGGACCTACGCGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                       <  1:2683824/140‑1 (MQ=14)

                                                                                gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCAGGCCCAGCCGGAGCGACAGGAGCAACGGCGGAAACAGGCACAGCCGCAGCGAc                                                       >  2:1550876/1‑140 (MQ=11)

                                                                                cccaCCGGCGCAGCCGGACCATCCGGAGCCCCAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                       <  1:4191091/137‑1 (MQ=14)

                                                                                    ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa                                                   >  2:3293911/1‑140 (MQ=255)

                                                                                       cgccaGCCGGACCCCCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                <  2:3451431/136‑1 (MQ=11)

                                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCcac                                              >  1:831362/1‑140 (MQ=255)

                                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                              >  1:4659351/1‑140 (MQ=255)

                                                                                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGACGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcag                                              >  2:4655481/1‑139 (MQ=255)

                                                                                          cggCCGGACCCCCCGGACCGACAGGCTCTAGCGGCGCACCCGGACCACCCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                             <  1:2791106/138‑1 (MQ=14)

                                                                                          acgCCGGACCAGCCGGCGCGACAGGAGCTAGGGGGTAAGCCGGACCCACCGGTGCGAGAGGTGCTACCGGGGAAGCCGGCGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                             <  1:1869061/138‑1 (MQ=14)

                                                                                          aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                             <  1:4260566/140‑1 (MQ=255)

                                                                                           aGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                            <  1:2983509/140‑1 (MQ=255)

                                                                                           aGCCGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCCACAGGAGCAACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                            <  1:394001/140‑1 (MQ=14)

                                                                                            gagggACGAGCCGGAGCCTCAGGAGCTCGCGGGCACGCAGGCCCGTGCTGAGCCCCAGGAGGTACCGGGGTACGGGGAGCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGTCCACCCGGAGCGACGGGAGCCACAgg                                           <  2:3688887/137‑1 (MQ=255)

                                                                                            gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                           >  2:3126558/1‑140 (MQ=255)

                                                                                            gCCGGACCACCCGGAGCCCCAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                           <  2:408639/140‑1 (MQ=255)

                                                                                            cccGGACCAGCCGGACCGCCTGGAGCTAACGGGGAAGCCGGAACATCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                           <  1:1038704/139‑1 (MQ=14)

                                                                                             ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCCGCCGGCGCGACAGGAGCGACCGGGGGTGGCGGACCCAGCGGCGCGGCGGGGGCCACAggg                                          >  2:762642/1‑140 (MQ=14)

                                                                                              cGGACCAGCCGGAGCGCCCGGCGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACGAACCGGAGCGACGGGAGCCACAgggg                                         <  2:1137325/140‑1 (MQ=14)

                                                                                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                         <  1:1176858/140‑1 (MQ=255)

                                                                                                 aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                           <  2:280002/87‑1 (MQ=38)

                                                                                                      ccGGCGCGACAGGACCTACCGGCGCAGCCGGACCCCCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                 <  2:4783464/140‑1 (MQ=17)

                                                                                                      ccGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                 <  1:4822867/140‑1 (MQ=255)

                                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                             <  1:3888896/140‑1 (MQ=255)

                                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                             <  1:1702082/140‑1 (MQ=255)

                                                                                                          aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCCCAGGACCAACAGCAGCGCCGGGAGCCACAGCCGCGACTgtatacg                             >  2:3792476/1‑134 (MQ=14)

                                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCACCTGGGGACGTCGGa                        >  2:525730/1‑140 (MQ=255)

                                                                                                               cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTTCTGGGGACGTCGGa                        >  2:2956085/1‑140 (MQ=255)

                                                                                                                aGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                       <  1:1629848/140‑1 (MQ=255)

                                                                                                                aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                       >  2:3608905/1‑140 (MQ=255)

                                                                                                                    gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGAGCGGAGCAACCGGGGCGGCCGGACCAACCGGACCGGCGGGAGCGACCGGGGCGTGGGGGGACGTCGGagcagc                   >  2:4519656/1‑140 (MQ=14)

                                                                                                                    gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                   <  2:2142236/140‑1 (MQ=255)

                                                                                                                     cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                  >  1:3261327/1‑140 (MQ=255)

                                                                                                                       aCCGGGGCACCCCGACCAACCGGAGCGACAGGCGCTACCGGGGCAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                <  1:3321630/140‑1 (MQ=18)

                                                                                                                       aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCgg                >  2:705977/1‑140 (MQ=255)

                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGAc          >  1:402315/1‑140 (MQ=255)

                                                                                                                             gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTCCAGGGGCCGACGTACCAGCCGCAGCGAc          >  2:3245290/1‑140 (MQ=17)

                                                                                                                             ccaaccGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGAc          <  2:1882460/136‑1 (MQ=255)

                                                                                                                                gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACAGGGCCCGTCGGACCAGCCGGAGCGACAgg       >  1:4370099/1‑140 (MQ=255)

                                                                                                                                gCCGGACCAACCGGAGCGACAGGAGCAACCGGCGCCCCCGGCCCAGGCGCAGCGTCCGCAGCGACGGGGGAAGCAGTACCAGCCCACGCGACTGGTGCCACAGGGGCTAGAGGGCAAGTCGGACCAGCCGGAGCGACggg       >  1:1180121/1‑140 (MQ=255)

                                                                                                                                cccgccccAACCCGAGCGACAGCAGCTACAGGGGCACCCGGACGATCCCGAGCGACAGGTGCTACCGGCGAAGCCGGACCATCACGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       <  2:4033404/134‑1 (MQ=17)

                                                                                                                                accGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGCGCCACAGGGGCTACTGGGGCCGTCGGACCAGCGGGAGCGACAgg       >  1:2209703/2‑140 (MQ=14)

                                                                                                                                accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       >  1:1981075/2‑140 (MQ=14)

                                                                                                                                accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg       >  1:4898753/2‑140 (MQ=14)

                                                                                                                                 ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                       <  1:1300716/59‑1 (MQ=35)

                                                                                                                                  cGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCCAcagcag     >  2:1907386/1‑140 (MQ=14)

                                                                                                                                  cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg     <  1:4702077/140‑1 (MQ=255)

CTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTA  >  NZ_CP034943/607537‑607809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`