BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,682	G→C	14.6%	A175A (GCG→GCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,682	0	G	C	14.6%	475.0 / 71.7	206	A175A (GCG→GCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (90/83); new base C (24/6); total (115/91)
Fisher's exact test for biased strand distribution p-value = 4.88e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 207 total aligned reads displayed.

GAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607545‑607818

gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGGGGGAGCgcc                                                                                                                                        >  1:270743/1‑138 (MQ=17)

gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAc                                                                                                                                        >  1:508697/1‑140 (MQ=255)

     cGGACCAGCCGGGGCCACGGGAGCGCCAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGAGCGGTGAAGCGGGCGCACCCGGAGCGAGCGGAg                                                                                                                                   >  2:195986/1‑140 (MQ=17)

     cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCGACCGGGGACACCGGACCACCCGGAGCGCCGGGAg                                                                                                                                   >  2:2160360/1‑140 (MQ=17)

     cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                   >  2:2136813/1‑140 (MQ=255)

        aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCAACAGGGGAAGCGGGAGCAACCGGAGCAGCAGGAGcga                                                                                                                                >  1:3695257/1‑138 (MQ=17)

             ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCAGGAACACCCGGAGCCACAGGATCTACAggt                                                                                                                           >  2:1883731/1‑139 (MQ=17)

             ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCggg                                                                                                                           >  2:3681864/1‑140 (MQ=255)

             ccGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                           <  1:3839947/140‑1 (MQ=255)

                  gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                   >  2:2600262/1‑127 (MQ=255)

                  gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                   <  1:2600262/127‑1 (MQ=255)

                       gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCggcc                                                                                                                 >  1:312025/1‑138 (MQ=255)

                       gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCATCCCGAGCGACCGGAGCAACCGGGCAAGcagtac                                                                                                                 >  1:1097021/1‑135 (MQ=14)

                           gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCGACGGGGGCCCCCGGCCCAgg                                                                                                             >  2:1281570/1‑139 (MQ=14)

                            ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                                        >  1:2550065/1‑112 (MQ=40)

                             cacaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGCGCAGCGGGACCAGCCg                                                                                                           >  2:4565805/1‑140 (MQ=255)

                                    gCTACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCCGCCGGCGCGACAGGAGCTACGGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                    <  2:3170491/140‑1 (MQ=255)

                                     cTACGGGGGAAGCCGCACCTCCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                   <  2:4072998/140‑1 (MQ=11)

                                      tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCGCAGGAACAGCCGGAGCgcgct                                                                                                  >  2:1521112/1‑136 (MQ=14)

                                       aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                 <  2:4620104/140‑1 (MQ=255)

                                       aCTGGGGAAGCCGGACCAGCCGGAGAGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGACGCCGGCCCAGCCGGAGCGACAgg                                                                                                 >  1:1137325/1‑140 (MQ=255)

                                         tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                               >  2:4838243/1‑140 (MQ=40)

                                            ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCACCCGGACCAGCCGCAGCGCCAGGAGcga                                                                                            >  1:1350998/1‑138 (MQ=14)

                                              aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGCCACAGGCGCAGCCGGAGCGACAGGAGCCAca                                                                                          >  2:688723/1‑139 (MQ=11)

                                                gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                                                                        >  2:1421724/1‑140 (MQ=255)

                                                     aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                   <  1:2156469/140‑1 (MQ=255)

                                                     aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAg                                                                                   >  2:2236103/1‑140 (MQ=255)

                                                     aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCGCCCGGGGATCCCGGAGACGCCGGAGCGACCGGAGCTACCGGGGAAg                                                                                   >  2:3252939/1‑140 (MQ=14)

                                                     aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                   >  2:2384181/1‑140 (MQ=255)

                                                     aCCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                   <  1:4842756/140‑1 (MQ=255)

                                                      cccgCCGGAGCGACCCGAGCGACGGGCGATGCCGGCCCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGc                                                                                  <  2:4124175/137‑1 (MQ=14)

                                                         gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGCCGGGAGCGACAGGCGCCGCCGGACCACCCGGCGCGACAGGAGCTACCTGGGCCGCCgg                                                                               >  2:4959455/1‑140 (MQ=14)

                                                          ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                            >  2:2916657/1‑110 (MQ=40)

                                                          ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                            <  1:2916657/110‑1 (MQ=40)

                                                                cGACAGGCGCTACCGGGGACGCCGGACCACCCGGCGCGACTGGAGCGACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                        <  1:806421/140‑1 (MQ=14)

                                                                   cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGa                                                                     >  1:2874534/1‑140 (MQ=255)

                                                                        gCTCCCGGCGAAGCCGGACCCGCCGGCGCGCCAGGACCTACGCGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                <  1:2683824/140‑1 (MQ=14)

                                                                        gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTACGCGGGACCACCCGGAGCGAc                                                                >  2:1917899/1‑140 (MQ=11)

                                                                        gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCAGGCCCAGCCGGAGCGACAGGAGCAACGGCGGAAACAGGCACAGCCGCAGCGAc                                                                >  2:1550876/1‑140 (MQ=11)

                                                                           cccGGCGAACCCGGCCCATCCGGCGCGACCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCTACCGGAGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                             <  2:3877932/139‑1 (MQ=14)

                                                                            ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa                                                            >  2:3293911/1‑140 (MQ=255)

                                                                                 gacgCCGGACCAGCCGGTGCGACTGGCGCTGGGGGGGTAGCGGGCCCAACCGGACCGACAGGCGCTCCCGGCCAAGCGGGAGCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGCGCGACGGGAGCcac                                                       <  1:4576608/137‑1 (MQ=2)

                                                                                 gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                       <  1:902748/140‑1 (MQ=255)

                                                                                 ccccccGCACCACCCGCACCGACCGGAGCTCCCGGCGAACCCGGACCCACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                       <  2:277412/136‑1 (MQ=14)

                                                                                  acgCCGGACCAGCCGGCGCGACAGGAGCTAGGGGGTAAGCCGGACCCACCGGTGCGAGAGGTGCTACCGGGGAAGCCGGCGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                      <  1:1869061/138‑1 (MQ=14)

                                                                                  aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                      <  1:4260566/140‑1 (MQ=255)

                                                                                   acccGGCCCAGCCGGACCCCCAGCAGCCCCCGGCGAAGCCGCACCAACCGGAGCGAAACGAGCTACCGGCGAAGCCTGTGGTGCGGGATCTTGTGTATATCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                     <  1:2398214/138‑1 (MQ=9)

                                                                                   aGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                     <  1:2983509/140‑1 (MQ=255)

                                                                                   aGCCGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCCACAGGAGCAACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                     <  1:394001/140‑1 (MQ=14)

                                                                                    gagggACGAGCCGGAGCCTCAGGAGCTCGCGGGCACGCAGGCCCGTGCTGAGCCCCAGGAGGTACCGGGGTACGGGGAGCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGTCCACCCGGAGCGACGGGAGCCACAgg                                                    <  2:3688887/137‑1 (MQ=255)

                                                                                    gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGACGGAGCGACAGGAGCTCCCGGGGCCGCCGGACCAACCGGAGCGACGGGAGCGCCAgg                                                    >  2:751189/1‑140 (MQ=14)

                                                                                    gCCGGACCACCCGGAGCCCCAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  2:408639/140‑1 (MQ=255)

                                                                                    cccGGACCAGCCGGAGCGACAGGAGGTTCCTGCGCACCCGGACCTACCGGAGACACAGGAGCTACCGGGGAAGCCGGAGCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  1:4240407/139‑1 (MQ=14)

                                                                                    cccGGACCAGCCGGACCGCCTGGAGCTAACGGGGAAGCCGGAACATCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  1:1038704/139‑1 (MQ=14)

                                                                                      cGGACCCCCCGGACCGACAGGCGCCACCGGGGAAGCCCGACCACCCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                  <  2:3092658/140‑1 (MQ=14)

                                                                                      cGGACCAGCCGGAGCGCCCGGCGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACGAACCGGAGCGACGGGAGCCACAgggg                                                  <  2:1137325/140‑1 (MQ=14)

                                                                                      cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                 <  2:599248/93‑1 (MQ=37)

                                                                                      cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                 >  1:599248/1‑93 (MQ=37)

                                                                                      cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                  <  1:1176858/140‑1 (MQ=255)

                                                                                      cGGACCAGCCGGAGCGACAGGAGCCACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                  <  1:4037746/140‑1 (MQ=255)

                                                                                         aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                    >  1:280002/1‑87 (MQ=38)

                                                                                              ccGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                          <  1:4822867/140‑1 (MQ=255)

                                                                                                  cgCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                      <  1:4655597/139‑1 (MQ=255)

                                                                                                  aGCGACAGGCGCTCCCGGGGACGCCGGACCAACCGGAGCCACAGGCGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                      <  1:3688209/140‑1 (MQ=17)

                                                                                                  aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                      <  1:3888896/140‑1 (MQ=255)

                                                                                                        aGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                <  1:1629848/140‑1 (MQ=255)

                                                                                                        aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                <  1:4013857/140‑1 (MQ=255)

                                                                                                        aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                >  2:3608905/1‑140 (MQ=255)

                                                                                                            gcgaCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                            <  2:4659351/137‑1 (MQ=255)

                                                                                                             cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                           >  1:3261327/1‑140 (MQ=255)

                                                                                                               aCCGGGGCACCCCGACCAACCGGAGCGACAGGCGCTACCGGGGCAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                         <  1:3321630/140‑1 (MQ=18)

                                                                                                                     gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCTGCCGGAGCGAc                   >  2:896758/1‑140 (MQ=255)

                                                                                                                     ccaaccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                   <  2:1866937/136‑1 (MQ=255)

                                                                                                                        gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGCGACGTCGGACCAGCCGGAGCGACAgg                >  1:4105353/1‑140 (MQ=255)

                                                                                                                        gCCGGACCAACCGGAGCGACAGGAGCAACCGGCGCCCCCGGCCCAGGCGCAGCGTCCGCAGCGACGGGGGAAGCAGTACCAGCCCACGCGACTGGTGCCACAGGGGCTAGAGGGCAAGTCGGACCAGCCGGAGCGACggg                >  1:1180121/1‑140 (MQ=255)

                                                                                                                        cccgccccAACCCGAGCGACAGCAGCTACAGGGGCACCCGGACGATCCCGAGCGACAGGTGCTACCGGCGAAGCCGGACCATCACGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                <  2:4033404/134‑1 (MQ=17)

                                                                                                                        accGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGCGCCACAGGGGCTACTGGGGCCGTCGGACCAGCGGGAGCGACAgg                >  1:2209703/2‑140 (MQ=14)

                                                                                                                        accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                >  1:4898753/2‑140 (MQ=14)

                                                                                                                        accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                >  1:1981075/2‑140 (MQ=14)

                                                                                                                         ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                <  1:1300716/59‑1 (MQ=35)

                                                                                                                          cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg              <  1:4702077/140‑1 (MQ=255)

                                                                                                                             aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGTGCGACTGGGGCCGTCGGACCAGCCGTAGCGACAGGAGCTa           >  2:2793537/1‑140 (MQ=255)

                                                                                                                             aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCtc           >  1:70158/1‑139 (MQ=255)

                                                                                                                              gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc          >  2:2564271/7‑140 (MQ=17)

                                                                                                                              gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc          >  2:283497/7‑140 (MQ=17)

                                                                                                                              gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc          >  1:1683396/7‑140 (MQ=17)

                                                                                                                              gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc          >  1:4397516/7‑140 (MQ=17)

                                                                                                                                 ccgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACTGGAGCCACCGGGGCAACTGTGGACGTCGGACCAGCCGTAGCGACATCAGCTACCgg       >  1:4175777/4‑140 (MQ=14)

                                                                                                                                 acgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg       <  1:4472165/137‑1 (MQ=18)

                                                                                                                                  ccGGTGCTACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg      <  1:4254998/140‑1 (MQ=255)

                                                                                                                                  ccGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCggg      <  1:4536889/140‑1 (MQ=255)

                                                                                                                                  ccGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg      <  1:1086762/140‑1 (MQ=255)

                                                                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCgg                                                    >  2:3516379/1‑94 (MQ=18)

                                                                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  1:3516379/94‑1 (MQ=40)

                                                                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  1:1761493/94‑1 (MQ=40)

                                                                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    >  2:1761493/1‑94 (MQ=40)

                                                                                                                                  ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg      <  1:2384181/140‑1 (MQ=255)

                                                                                                                                      aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg  >  1:413030/1‑140 (MQ=255)

                                                                                                                                      aGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAGAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAg  >  1:4695161/1‑140 (MQ=18)

                                                                                                                                      aGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAg  >  1:247967/1‑140 (MQ=18)

GAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607545‑607818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`