BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,688	A→G	12.3%	G177G (GGA→GGG)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,688	0	A	G	12.3%	468.9 / 56.0	200	G177G (GGA→GGG)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (93/75); new base G (22/2); total (115/85)
Fisher's exact test for biased strand distribution p-value = 5.66e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

Only 100 of 200 total aligned reads displayed.

CGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAC  >  NZ_CP034943/607550‑607824

cGGACCAGCCGGGGCCACGGGAGCGCCAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGAGCGGTGAAGCGGGCGCACCCGGAGCGAGCGGAg                                                                                                                                         >  2:195986/1‑140 (MQ=17)

      cgCCGGGGCCACGGGAGCCCCCGGGGCTACTGGGCAAGCCGGCCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAcag                                                                                                                                   <  1:3569450/139‑3 (MQ=11)

        ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCAGGAACACCCGGAGCCACAGGATCTACAggt                                                                                                                                 >  2:1883731/1‑139 (MQ=17)

        ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCTCCCggg                                                                                                                                 >  2:3681864/1‑140 (MQ=255)

        ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCAGGCCCACGCGCAGCGACAGGAGCCACAggc                                                                                                                                 >  2:4057652/1‑139 (MQ=14)

            ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCggcgccg                                                                                                                             >  2:97875/1‑135 (MQ=255)

             gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                         >  2:2600262/1‑127 (MQ=255)

             gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                         <  1:2600262/127‑1 (MQ=255)

                  gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCggcc                                                                                                                       >  1:312025/1‑138 (MQ=255)

                      gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCGACGGGGGCCCCCGGCCCAgg                                                                                                                   >  2:1281570/1‑139 (MQ=14)

                        cacaGGGGCTACTGGGGAAGCCGGAGCCGCCGGAGCGCCAGGAGCGACCGGGGACGCCGGACCAGCCGGGGCGACAGGCGGGGGCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCGACGGGGGCAGCCGGAccgggcg                                                                                                                 >  1:516161/1‑135 (MQ=14)

                        cacaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                                                 >  2:651091/1‑140 (MQ=255)

                        cacaGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGCCGCCGGCCCACCCGGCGCGCCCGGAGCTCCCGGGGCCGCCGGCCGAGCCg                                                                                                                 >  2:3259339/1‑140 (MQ=14)

                               gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCAACCGGCGAGTCCGGACAATCCGgcgctcc                                                                                                          >  1:2491382/1‑137 (MQ=11)

                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACAGGGGAAGCCGGACCACCCGGAGCGCCAgg                                                                                                       >  2:4584681/1‑140 (MQ=255)

                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                       >  1:951296/1‑140 (MQ=255)

                                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCACCCGGACCAGCCGCAGCGCCAGGAGcga                                                                                                  >  1:1350998/1‑138 (MQ=14)

                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGACCGACAGGAGCTACAgg                                                                                              >  2:4079024/1‑140 (MQ=255)

                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCCGCCGGAGCCACCGGAGCTACggg                                                                                              >  2:2779377/1‑140 (MQ=14)

                                           gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAACCGGACCAGCCGGAGCTAAAGGAGCTACCGGGGAAGCCGGAACAACCGGAGCGACAGGAGCTACCGGGGAAGCCGCACCATCCGGAGGGACAGGAGCTACCgg                                                                                              >  1:1117376/1‑140 (MQ=14)

                                            ccGGACCACCCGGCGCCCCAGGAGCAGCCGGAGCCCCCGGAGCACCCGGAGCGACCGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                             <  2:4852987/140‑1 (MQ=14)

                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGATCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         >  1:759057/1‑140 (MQ=255)

                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         <  1:2156469/140‑1 (MQ=255)

                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         <  1:4076043/140‑1 (MQ=255)

                                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         <  1:2421870/140‑1 (MQ=255)

                                                aCCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                         <  1:4842756/140‑1 (MQ=255)

                                                     ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                  <  1:2916657/110‑1 (MQ=40)

                                                     ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                       >  1:115386/1‑121 (MQ=40)

                                                              cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGa                                                                           >  1:4250824/1‑140 (MQ=255)

                                                              cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGa                                                                           >  1:2874534/1‑140 (MQ=255)

                                                                   gCTCCCGGGGAAGCCGGAGCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                      <  1:2392482/140‑1 (MQ=255)

                                                                   gCTCCCGGGGAAGCCGGACCCGCCGGAGGGACAGGCGCTCCGGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                      <  1:609052/140‑1 (MQ=11)

                                                                   gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTACGCGGGACCACCCGGAGCGAc                                                                      >  2:1917899/1‑140 (MQ=11)

                                                                   cccaCCGGCGCAGCCGGACCATCCGGAGCCCCAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                      <  1:4191091/137‑1 (MQ=14)

                                                                          cgccaGCCGGACCCCCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                               <  2:3451431/136‑1 (MQ=11)

                                                                            gacgCCGGACCAGCCGGTGCGACTGGCGCTGGGGGGGTAGCGGGCCCAACCGGACCGACAGGCGCTCCCGGCCAAGCGGGAGCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGCGCGACGGGAGCcac                                                             <  1:4576608/137‑1 (MQ=2)

                                                                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCcac                                                             >  1:831362/1‑140 (MQ=255)

                                                                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGACGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcag                                                             >  2:4655481/1‑139 (MQ=255)

                                                                            ccccccGCACCACCCGCACCGACCGGAGCTCCCGGCGAACCCGGACCCACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                             <  2:277412/136‑1 (MQ=14)

                                                                             acgCCGGACCAGCCGGCGCGACAGGAGCTAGGGGGTAAGCCGGACCCACCGGTGCGAGAGGTGCTACCGGGGAAGCCGGCGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                            <  1:1869061/138‑1 (MQ=14)

                                                                             aaGCCGGACCAGCCGGAGCGACAGGAGCTTCCGTGGACGCCTGACCAACAGGAGCGACAGGAGCTACCGGGGATGCCGGACCATCGGGATCGACAGGAGCTCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                            <  1:4260305/140‑1 (MQ=14)

                                                                             aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                            >  2:152717/1‑140 (MQ=255)

                                                                             aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCcaca                                                            >  2:2485346/1‑140 (MQ=255)

                                                                              aGCCGGACCTGCCGGAGCTACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGATCAACCGGAGCGACGGGAGCCACAg                                                           <  1:4355876/140‑1 (MQ=255)

                                                                              aGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                           <  1:2983509/140‑1 (MQ=255)

                                                                               gagggACGAGCCGGAGCCTCAGGAGCTCGCGGGCACGCAGGCCCGTGCTGAGCCCCAGGAGGTACCGGGGTACGGGGAGCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGTCCACCCGGAGCGACGGGAGCCACAgg                                                          <  2:3688887/137‑1 (MQ=255)

                                                                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                          >  2:3126558/1‑140 (MQ=255)

                                                                               cccGGACCAGCCGGACCGCCTGGAGCTAACGGGGAAGCCGGAACATCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                          <  1:1038704/139‑1 (MQ=14)

                                                                                ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCCGCCGGCGCGACAGGAGCGACCGGGGGTGGCGGACCCAGCGGCGCGGCGGGGGCCACAggg                                                         >  2:762642/1‑140 (MQ=14)

                                                                                 cGGACCAGCCGGAGCGCCCGGCGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACGAACCGGAGCGACGGGAGCCACAgggg                                                        <  2:1137325/140‑1 (MQ=14)

                                                                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCCGCCGGAGCGACAGGCGCACCCGGGGCAGCCGGCCACACCGGAGCGACGGGAGCCGCCgggg                                                        >  2:2900014/1‑140 (MQ=11)

                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                          >  1:280002/1‑87 (MQ=38)

                                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                          <  2:280002/87‑1 (MQ=38)

                                                                                         ccGGCGCGACAGGACCTACCGGCGCAGCCGGACCCCCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                <  2:4783464/140‑1 (MQ=17)

                                                                                         ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                  <  1:1178614/74‑1 (MQ=39)

                                                                                         ccGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                <  1:4822867/140‑1 (MQ=255)

                                                                                             cgCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                            <  1:4655597/139‑1 (MQ=255)

                                                                                             aGCGACAGGCGCTCCCGGGGACGCCGGACCAACCGGAGCCACAGGCGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                            <  1:3688209/140‑1 (MQ=17)

                                                                                             aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCCCAGGACCAACAGCAGCGCCGGGAGCCACAGCCGCGACTgtatacg                                            >  2:3792476/1‑134 (MQ=14)

                                                                                                  cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCACCTGGGGACGTCGGa                                       >  2:525730/1‑140 (MQ=255)

                                                                                                  cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTTCTGGGGACGTCGGa                                       >  2:2956085/1‑140 (MQ=255)

                                                                                                   aGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                      <  1:1629848/140‑1 (MQ=255)

                                                                                                   aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGACGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                      >  1:2685864/1‑140 (MQ=255)

                                                                                                   aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                      >  2:3608905/1‑140 (MQ=255)

                                                                                                       gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGAGCGGAGCAACCGGGGCGGCCGGACCAACCGGACCGGCGGGAGCGACCGGGGCGTGGGGGGACGTCGGagcagc                                  >  2:4519656/1‑140 (MQ=14)

                                                                                                       gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                  <  2:2142236/140‑1 (MQ=255)

                                                                                                        cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                 >  1:3261327/1‑140 (MQ=255)

                                                                                                          aCCGGGGCACCCCGACCAACCGGAGCGACAGGCGCTACCGGGGCAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                               <  1:3321630/140‑1 (MQ=18)

                                                                                                          aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCgg                               >  2:705977/1‑140 (MQ=255)

                                                                                                                gAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGGCGGCCCAGCCGGAGCTACTGGAGCTAACGGGGCCTCGGGACCAACCTGAGCTACGGGAGCCACCGGCGCGAGGGGGGCCGTCGGAGCTGGGGGAGCGAc                         >  2:1436385/1‑140 (MQ=9)

                                                                                                                gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGAc                         >  1:402315/1‑140 (MQ=255)

                                                                                                                gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGAc                         >  1:3458977/1‑140 (MQ=255)

                                                                                                                gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTCCAGGGGCCGACGTACCAGCCGCAGCGAc                         >  2:3245290/1‑140 (MQ=17)

                                                                                                                ccaaccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                         <  2:1866937/136‑1 (MQ=255)

                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                         <  2:1666845/57‑1 (MQ=35)

                                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                      >  1:2359937/1‑140 (MQ=255)

                                                                                                                   ccccgcccAACCGGCGCGCCAGCAGCCACCGGCGAAGCCGGACCAGCCGGAGCGCCCGGAGCTCCCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                      <  2:4087676/134‑1 (MQ=18)

                                                                                                                   accGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGCGCCACAGGGGCTACTGGGGCCGTCGGACCAGCGGGAGCGACAgg                      >  1:2209703/2‑140 (MQ=14)

                                                                                                                   accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                      >  1:1981075/2‑140 (MQ=14)

                                                                                                                   accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                      >  1:4898753/2‑140 (MQ=14)

                                                                                                                   accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                      >  1:3867998/2‑140 (MQ=14)

                                                                                                                    ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                     >  2:2228271/1‑140 (MQ=14)

                                                                                                                    ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                      <  1:1300716/59‑1 (MQ=35)

                                                                                                                     cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                    <  1:4702077/140‑1 (MQ=255)

                                                                                                                        cccACCCGGAGCCCCAGGAGCCCCCGGCCACGCGGGACCATCCGGAGCCACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                 <  2:2256731/139‑1 (MQ=17)

                                                                                                                         gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                >  2:2564271/7‑140 (MQ=17)

                                                                                                                         gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                >  1:4126977/7‑140 (MQ=17)

                                                                                                                         gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                >  1:1683396/7‑140 (MQ=17)

                                                                                                                         gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                >  1:4173635/7‑140 (MQ=17)

                                                                                                                         gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                >  1:4397516/7‑140 (MQ=17)

                                                                                                                         gcgacgggAGCCACAGGGGCTACAGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCTGACCAACCGGAGCGACGGCAGCCACAGGGGCTAATGGGTACGTCTGACCAGCCGGAGCGACAGGAGCTAc                >  1:3898770/7‑140 (MQ=14)

                                                                                                                            acgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg             <  1:4472165/137‑1 (MQ=18)

                                                                                                                            acgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGCCGTCGGACCAGCCGGAGCGACAGGCGCGCCCgg             >  2:3934249/4‑140 (MQ=14)

                                                                                                                             ccGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCggg            <  1:4536889/140‑1 (MQ=255)

                                                                                                                             ccGGAGCGACAGGCGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg            <  1:3768221/140‑1 (MQ=255)

                                                                                                                             ccGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg            <  1:1086762/140‑1 (MQ=255)

                                                                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCgg                                                          >  2:3516379/1‑94 (MQ=18)

                                                                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                          <  1:1761493/94‑1 (MQ=40)

                                                                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGGCGGACCAGCCGCAGCGACAGGAGCTACCGGGGacg        >  1:3940049/1‑138 (MQ=21)

                                                                                                                                 aGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAGAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAg        >  1:4695161/1‑140 (MQ=18)

CGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAC  >  NZ_CP034943/607550‑607824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`