BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	27.4%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	27.4%	274.8 / 129.5	220	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (79/77); new base C (53/7); total (135/85)
Fisher's exact test for biased strand distribution p-value = 1.45e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 221 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAC  >  NZ_CP034943/607567‑607837

     gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCGACGGGGGCCCCCGGCCCAgg                                                                                                                                >  2:1281570/1‑139 (MQ=14)

       cacaGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGCCGCCGGCCCACCCGGCGCGCCCGGAGCTCCCGGGGCCGCCGGCCGAGCCg                                                                                                                              >  2:3259339/1‑140 (MQ=14)

              gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCAACCGGCGAGTCCGGACAATCCGgcgctcc                                                                                                                       >  1:2491382/1‑137 (MQ=11)

                tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAg                                                                                                                     >  1:4620104/1‑140 (MQ=255)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACAGGGGAAGCCGGACCACCCGGAGCGCCAgg                                                                                                                    >  2:4584681/1‑140 (MQ=255)

                 aCTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGACGGAGCGACAgg                                                                                                                    >  1:3092658/1‑140 (MQ=255)

                   tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                  >  2:4838243/1‑140 (MQ=40)

                   ccgcgcaGCCGCACCACCCGGAGCGACAGGAGCCACCGGCGACCCCGGCGCCGCCGGAGCCGCCGGAGCTCCCGGAGCTCCCGGAGCCACCGGAGCGACAGGGGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                  <  1:219764/134‑1 (MQ=9)

                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCACCCGGACCAGCCGCAGCGCCAGGAGcga                                                                                                               >  1:1350998/1‑138 (MQ=14)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGCCACAGGCGCAGCCGGAGCGACAGGAGCCAca                                                                                                             >  2:688723/1‑139 (MQ=11)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCAGCGCAGCCGGCCCAGCCGGAGCGACCGGAGCtcacgg                                                                                                           >  1:282252/1‑135 (MQ=11)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGACCGACAGGAGCTACAgg                                                                                                           >  2:4079024/1‑140 (MQ=255)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCCGCCGGAGCCACCGGAGCTACggg                                                                                                           >  2:2779377/1‑140 (MQ=14)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAACCGGACCAGCCGGAGCTAAAGGAGCTACCGGGGAAGCCGGAACAACCGGAGCGACAGGAGCTACCGGGGAAGCCGCACCATCCGGAGGGACAGGAGCTACCgg                                                                                                           >  1:1117376/1‑140 (MQ=14)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAg                                                                                                      >  2:2236103/1‑140 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGGCGGAGCGCCAGGAGCGACCGGCGAGGCCGGAGCAGCCGGAGCTACAGGAGCCACCggagccg                                                                                                      >  2:1927366/1‑135 (MQ=11)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                      <  1:2421870/140‑1 (MQ=255)

                                ccgcccGGATCGACAGGCGCTTCCGGAGAAGCCGGAGCCACCGGAGCGACAGGTGCTACCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                     <  2:2583550/136‑1 (MQ=14)

                                cccgCCGGAGCGACCCGAGCGACGGGCGATGCCGGCCCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGc                                                                                                     <  2:4124175/137‑1 (MQ=14)

                                   gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGCCGGGAGCGACAGGCGCCGCCGGACCACCCGGCGCGACAGGAGCTACCTGGGCCGCCgg                                                                                                  >  2:4959455/1‑140 (MQ=14)

                                    ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                               >  2:2916657/1‑110 (MQ=40)

                                    ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                    >  1:115386/1‑121 (MQ=40)

                                     cGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                <  1:4838243/140‑1 (MQ=255)

                                        aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACCGGGGCACCCGGCCCAGCCGGACCCCCCGGAGCGACCGGCGAACCCGGACAAGCCGGACCGACCGGAGCGACCGGGGACGCCGGAGCac                                                                                             >  1:4858587/1‑139 (MQ=2)

                                         gCGACAGGCGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                            <  1:4575096/140‑1 (MQ=255)

                                          cGACAGGCGCTACCGGGGACGCCGGACCACCCGGCGCGACTGGAGCGACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                           <  1:806421/140‑1 (MQ=14)

                                          cGACAGGAGCTCCCGGGGATGCCGGTCCATCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                           <  1:4822711/140‑1 (MQ=14)

                                             cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGa                                                                                        >  1:4250824/1‑140 (MQ=255)

                                                  gCTCCCGGGGAAGCCGGAGCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                   <  1:2392482/140‑1 (MQ=255)

                                                  gCTCCCGGCGAAGCCGGACCCGCCGGCGCGCCAGGACCTACGCGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                   <  1:2683824/140‑1 (MQ=14)

                                                         cgccaGCCGGACCCCCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                            <  2:3451431/136‑1 (MQ=11)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          >  1:2142236/1‑140 (MQ=255)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  1:902748/140‑1 (MQ=255)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          >  1:4659351/1‑140 (MQ=255)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGACGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcag                                                                          >  2:4655481/1‑139 (MQ=255)

                                                           ccccccGCACCACCCGCACCGACCGGAGCTCCCGGCGAACCCGGACCCACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  2:277412/136‑1 (MQ=14)

                                                            cggCCGGACCCCCCGGACCGACAGGCTCTAGCGGCGCACCCGGACCACCCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         <  1:2791106/138‑1 (MQ=14)

                                                            acgCCGGACCAGCCGGCGCGACAGGAGCTAGGGGGTAAGCCGGACCCACCGGTGCGAGAGGTGCTACCGGGGAAGCCGGCGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         <  1:1869061/138‑1 (MQ=14)

                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTTCCGTGGACGCCTGACCAACAGGAGCGACAGGAGCTACCGGGGATGCCGGACCATCGGGATCGACAGGAGCTCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         <  1:4260305/140‑1 (MQ=14)

                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         >  2:152717/1‑140 (MQ=255)

                                                             aGCCGGACCTGCCGGAGCTACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGATCAACCGGAGCGACGGGAGCCACAg                                                                        <  1:4355876/140‑1 (MQ=255)

                                                             aGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                                        <  1:2983509/140‑1 (MQ=255)

                                                             aGCCGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGACCAACCGGAGCCACAGGAGCAACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                                        <  1:394001/140‑1 (MQ=14)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       >  2:3126558/1‑140 (MQ=255)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGACGGAGCGACAGGAGCTCCCGGGGCCGCCGGACCAACCGGAGCGACGGGAGCGCCAgg                                                                       >  2:751189/1‑140 (MQ=14)

                                                              cccGGACCAGCCGGACCGCCTGGAGCTAACGGGGAAGCCGGAACATCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       <  1:1038704/139‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGCCCGGCGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACGAACCGGAGCGACGGGAGCCACAgggg                                                                     <  2:1137325/140‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                    <  2:599248/93‑1 (MQ=37)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                     <  1:3293911/140‑1 (MQ=255)

                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                       <  2:280002/87‑1 (MQ=38)

                                                                    ccAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTAc                                                                 <  1:3126558/140‑1 (MQ=255)

                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                               <  1:1178614/74‑1 (MQ=39)

                                                                            aGCGACAGGCGCTCCCGGGGACGCCGGACCAACCGGAGCCACAGGCGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                         <  1:3688209/140‑1 (MQ=17)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                         <  1:3888896/140‑1 (MQ=255)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                         <  1:1702082/140‑1 (MQ=255)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCCCAGGACCAACAGCAGCGCCGGGAGCCACAGCCGCGACTgtatacg                                                         >  2:3792476/1‑134 (MQ=14)

                                                                                  aGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                   <  1:1629848/140‑1 (MQ=255)

                                                                                  aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                   >  2:3608905/1‑140 (MQ=255)

                                                                                      gcgaCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                               <  2:4659351/137‑1 (MQ=255)

                                                                                      gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGAGCGGAGCAACCGGGGCGGCCGGACCAACCGGACCGGCGGGAGCGACCGGGGCGTGGGGGGACGTCGGagcagc                                               >  2:4519656/1‑140 (MQ=14)

                                                                                      gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                               <  2:2142236/140‑1 (MQ=255)

                                                                                              accaaccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                       >  1:4914637/6‑140 (MQ=11)

                                                                                               gAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGGCGGCCCAGCCGGAGCTACTGGAGCTAACGGGGCCTCGGGACCAACCTGAGCTACGGGAGCCACCGGCGCGAGGGGGGCCGTCGGAGCTGGGGGAGCGAc                                      >  2:1436385/1‑140 (MQ=9)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGAc                                      >  1:402315/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCTGCCGGAGCGAc                                      >  2:896758/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGAc                                      >  1:3458977/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTCCAGGGGCCGACGTACCAGCCGCAGCGAc                                      >  2:3245290/1‑140 (MQ=17)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                                      >  1:1666845/1‑57 (MQ=35)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                   >  1:2359937/1‑140 (MQ=255)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGCGACGTCGGACCAGCCGGAGCGACAgg                                   >  1:4105353/1‑140 (MQ=255)

                                                                                                  cccgccccAACCCGAGCGACAGCAGCTACAGGGGCACCCGGACGATCCCGAGCGACAGGTGCTACCGGCGAAGCCGGACCATCACGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                   <  2:4033404/134‑1 (MQ=17)

                                                                                                  accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                   >  1:3381798/2‑140 (MQ=14)

                                                                                                  accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                   >  1:1981075/2‑140 (MQ=14)

                                                                                                  accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                   >  1:3867998/2‑140 (MQ=14)

                                                                                                    cGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCCAcagcag                                 >  2:1907386/1‑140 (MQ=14)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                 <  1:2911101/140‑1 (MQ=255)

                                                                                                       aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCtc                              >  1:70158/1‑139 (MQ=255)

                                                                                                        gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                             >  1:4397516/7‑140 (MQ=17)

                                                                                                           ccgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACTGGAGCCACCGGGGCAACTGTGGACGTCGGACCAGCCGTAGCGACATCAGCTACCgg                          >  1:4175777/4‑140 (MQ=14)

                                                                                                           acgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGCCGTCGGACCAGCCGGAGCGACAGGCGCGCCCgg                          >  2:3934249/4‑140 (MQ=14)

                                                                                                            ccGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCggg                         <  1:4536889/140‑1 (MQ=255)

                                                                                                            ccGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                         <  1:1086762/140‑1 (MQ=255)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCgg                                                                       >  2:3516379/1‑94 (MQ=18)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       >  2:1761493/1‑94 (MQ=40)

                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                     >  1:413030/1‑140 (MQ=255)

                                                                                                                aGCCACAGGGGCTACTGGGGACGTCGGACCAGCCTGAGCGACAGGAGCTACCGGGGAATCCGGACCAACCGGATCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAg                     >  1:4695506/1‑140 (MQ=18)

                                                                                                                     cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                >  2:2558844/1‑140 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       <  2:2282271/80‑1 (MQ=255)

                                                                                                                           cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCGACTGGGGACGTCGGACCAGCCGGCGCCACAGGAGCTACCGGGGAAGCCGGACCAAcc          >  2:4258038/1‑140 (MQ=18)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  1:406385/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  1:1762495/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  1:2256835/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcagc        >  2:3687927/4‑137 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  2:667155/4‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGACGGACCAACCgg        >  1:576785/4‑140 (MQ=17)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg      >  2:4277138/1‑140 (MQ=255)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg      >  2:3764903/1‑140 (MQ=255)

                                                                                                                                ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGc     >  2:1665334/1‑140 (MQ=255)

                                                                                                                                  ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAACCGGACCACCAGCAGCGa   >  2:3078775/1‑140 (MQ=17)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  >  1:1312429/1‑140 (MQ=255)

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAC  >  NZ_CP034943/607567‑607837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`