BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	52,263	T→G	5.7%	intergenic (+24/‑187)	prtG → / → EO946_RS00280	sporulation‑specific protease PrtG/Veg family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	52,263	0	T	G	5.7%	824.9 / 20.4	244	intergenic (+24/‑187)	prtG/EO946_RS00280	sporulation‑specific protease PrtG/Veg family protein
Reads supporting (aligned to +/- strand): ref base T (161/68); new base G (7/7); total (169/75)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 246 total aligned reads displayed.

TGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTG  >  NZ_CP034943/52124‑52402

tGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGAt                                                                                                                                             <  2:4036638/140‑1 (MQ=255)

 ggAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATc                                                                                                                                            <  2:423344/140‑1 (MQ=255)

    aCGAATCAATGTATTGGAAGTGCTCCCAAATACGGTGACACGAGAGAAAGGGCTTGGAGGTATTGAAACAATAGGAGTGGTGAGAATTGGAATGCCTTATAAGTCGAATTAACAGTTGAAAACCTGTATAGGAGATCTAt                                                                                                                                         >  1:1657622/1‑140 (MQ=255)

    aCGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGGGATCTAt                                                                                                                                         >  1:4965575/1‑140 (MQ=255)

    aCGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTAt                                                                                                                                         >  1:4613604/1‑140 (MQ=255)

      gAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAATGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGACTTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGc                                                                                                                                       >  2:1032280/1‑140 (MQ=255)

             tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                                <  2:563385/140‑1 (MQ=255)

             tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                                <  2:3506644/140‑1 (MQ=255)

             tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                                <  2:2186185/140‑1 (MQ=255)

             tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                                <  2:3180352/140‑1 (MQ=255)

             tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                                >  1:882154/1‑140 (MQ=255)

             tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                                <  2:1373241/140‑1 (MQ=255)

             tGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATCGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGtttt                                                                                                                                <  2:4886009/140‑1 (MQ=255)

                 tGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACATGTATAGGAGATCTATGCGGGTTttttat                                                                                                                            >  2:2422530/1‑140 (MQ=255)

                  gggAAGTGCTCCGTAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                           >  2:2980197/1‑140 (MQ=255)

                  gggAAGTGCTCCGTAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                           >  2:3804338/1‑140 (MQ=255)

                  gggAAGTGCTCCGTAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                           >  2:4355104/1‑140 (MQ=255)

                  gggAAGTGCTCCGTAATACACTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTTCTGAGAATTGGTATGCCTTATAAGTCCAATTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTttttatt                                                                                                                           >  2:141025/1‑140 (MQ=255)

                  gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                           >  2:805602/1‑140 (MQ=255)

                  gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                           <  2:4189716/140‑1 (MQ=255)

                  gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                           >  2:2583970/1‑140 (MQ=255)

                  gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGAATAGGAGATCTATGCGGGTTttttatt                                                                                                                           >  2:1999323/1‑140 (MQ=255)

                  gggAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACATGTATAGGAGATCTATGCGGGTTttttatt                                                                                                                           >  2:3871986/1‑140 (MQ=255)

                       gTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACa                                                                                                                      >  1:389925/1‑140 (MQ=255)

                       gTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCGGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACa                                                                                                                      >  1:3755776/1‑140 (MQ=255)

                         gCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATa                                                                                                                    >  1:2505838/1‑140 (MQ=255)

                         gCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATa                                                                                                                    >  1:4060850/1‑140 (MQ=255)

                         gCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATa                                                                                                                    >  1:479949/1‑140 (MQ=255)

                         gCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATa                                                                                                                    <  1:2402742/140‑1 (MQ=255)

                          cTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATaa                                                                                                                   >  2:1880004/1‑140 (MQ=255)

                           tCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAAt                                                                                                                  >  1:1384251/1‑140 (MQ=255)

                           tCCGAAATACGCTGACAAGAGAGAAAGGGCGTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATATATGCGGGTTTTTTATTTTACATAAt                                                                                                                  >  2:3626330/1‑140 (MQ=255)

                               aaaTACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATa                                                                                                              <  2:1744081/140‑1 (MQ=255)

                               aaaTACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATa                                                                                                              >  2:1152799/1‑140 (MQ=255)

                                    cGCTGACAAGAGAGAAAGGGCTTGGAGGTCTTGAAACAAGCGGAGTGCTGAGAAGTGGAATGCCTTCGAAGTCTAATTAACCGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATaa                                                                                                         >  1:57938/1‑140 (MQ=255)

                                    cGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATaa                                                                                                         >  2:210866/1‑140 (MQ=255)

                                     gCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAAt                                                                                                        <  1:4542529/140‑1 (MQ=255)

                                     gCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAAt                                                                                                        >  1:2968783/1‑140 (MQ=255)

                                         aCAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAc                                                                                                    >  2:2554503/1‑140 (MQ=255)

                                         aCAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAc                                                                                                    >  2:1609940/1‑140 (MQ=255)

                                         aCAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAc                                                                                                    >  2:4420109/1‑140 (MQ=255)

                                         aCAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTAc                                                                                                    >  2:367795/1‑140 (MQ=255)

                                                  aaaGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTg                                                                                           >  2:1791710/1‑140 (MQ=255)

                                                  aaaGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTg                                                                                           >  1:3157684/1‑140 (MQ=255)

                                                   aaGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGc                                                                                          >  1:2852741/1‑140 (MQ=255)

                                                     gggCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCgg                                                                                        >  1:3424142/1‑140 (MQ=255)

                                                       gCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGaa                                                                                      >  1:975457/1‑140 (MQ=255)

                                                        cTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCGTTATAAGTCTAATTAACCGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATGTTACATAATGATACATAATTTACCGAAACTTGCGGaaa                                                                                     >  1:548884/1‑140 (MQ=255)

                                                          tGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAaat                                                                                   >  2:1216940/1‑140 (MQ=255)

                                                                 aTTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAg                                                                            >  2:4092645/1‑140 (MQ=255)

                                                                 aTTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAg                                                                            >  2:4450823/1‑140 (MQ=255)

                                                                 aTTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATGTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAg                                                                            >  1:2927481/1‑140 (MQ=255)

                                                                  ttGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAgg                                                                           >  1:279641/1‑140 (MQ=255)

                                                                          agagGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAg                                                                   >  1:1855026/1‑140 (MQ=255)

                                                                                 tGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTg                                                            >  2:3391530/1‑140 (MQ=255)

                                                                                 tGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTg                                                            >  1:3681732/1‑140 (MQ=255)

                                                                                 tGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTg                                                            >  1:2060528/1‑140 (MQ=255)

                                                                                  gCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGt                                                           <  1:1516497/140‑1 (MQ=255)

                                                                                   cTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTc                                                          >  1:1979164/1‑140 (MQ=255)

                                                                                     gagaATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCaa                                                        <  1:2052425/140‑1 (MQ=255)

                                                                                       gaATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCaaaa                                                      <  1:3236556/140‑1 (MQ=255)

                                                                                       gaATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCaaaa                                                      <  1:1838586/140‑1 (MQ=255)

                                                                                         aTTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAaata                                                    <  2:2371407/140‑1 (MQ=255)

                                                                                               aTGCCTTATAAGTCTAATTAACAGTTGACAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACAGAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTa                                              <  1:3974798/140‑1 (MQ=255)

                                                                                                tGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTAt                                             >  1:770970/1‑140 (MQ=255)

                                                                                                  ccTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGa                                                                          <  2:736398/109‑1 (MQ=255)

                                                                                                  ccTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTg                                           >  1:652915/1‑140 (MQ=255)

                                                                                                    ttATAAGTCTAATTAACAGTTGAACACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGAc                                         <  2:4730045/140‑1 (MQ=255)

                                                                                                    ttATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGAc                                         >  2:4861837/1‑140 (MQ=255)

                                                                                                    ttATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGAc                                         <  1:1791710/140‑1 (MQ=255)

                                                                                                      ataAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACaa                                       <  2:2780760/140‑1 (MQ=255)

                                                                                                       taAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAAc                                      <  2:3433357/140‑1 (MQ=255)

                                                                                                         aGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGt                                    <  2:4295820/140‑1 (MQ=255)

                                                                                                         aGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGt                                    <  1:113519/140‑1 (MQ=255)

                                                                                                         aGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGt                                    <  2:278428/140‑1 (MQ=255)

                                                                                                         aGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGt                                    <  1:4092645/140‑1 (MQ=255)

                                                                                                           tCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCt                                  <  1:805602/140‑1 (MQ=255)

                                                                                                            cTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGTGGAATCATAGAATTTTGTCAAAATAATTTTATTTACAACGTCtt                                 >  2:3948030/1‑140 (MQ=255)

                                                                                                            cTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAAtt                                                               >  2:3062833/1‑110 (MQ=255)

                                                                                                            cTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAAtt                                                               <  1:3062833/110‑1 (MQ=255)

                                                                                                             tAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCtta                                >  1:498516/1‑140 (MQ=255)

                                                                                                               aTTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                              >  1:2693422/1‑140 (MQ=255)

                                                                                                               aTTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAACATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                              <  1:4355104/140‑1 (MQ=255)

                                                                                                               aTTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAACATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                              <  1:4429198/140‑1 (MQ=255)

                                                                                                               aTTAACAGTTGAAAACCTGCATAGGAGAGCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAACATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCttatt                              <  1:4572994/140‑1 (MQ=255)

                                                                                                                   aCAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACAGACACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACg                          <  2:833394/140‑1 (MQ=255)

                                                                                                                     aGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGtt                        >  1:1420603/1‑140 (MQ=255)

                                                                                                                                 gTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTaa            >  1:10863/1‑140 (MQ=255)

                                                                                                                                 gTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTaa            >  1:2101865/1‑140 (MQ=255)

                                                                                                                                 gTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTaa            >  1:1580560/1‑140 (MQ=255)

                                                                                                                                 gTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTAGTAACGTTGATATAATTTaa            >  1:2159294/1‑140 (MQ=255)

                                                                                                                                 gTATAGGAGATCTATGCGGGTTTTTTATGTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTaa            >  1:1205350/1‑140 (MQ=255)

                                                                                                                                       gagaTCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCaaaa                                                      >  1:4740258/1‑92 (MQ=255)

                                                                                                                                       gagaTCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTa      >  2:884885/1‑140 (MQ=255)

                                                                                                                                       gagaTCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTa      >  2:3303156/1‑140 (MQ=255)

                                                                                                                                       gagaTCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTa      >  2:790456/1‑140 (MQ=255)

                                                                                                                                       gagaTCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATAGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTa      >  2:3999689/1‑140 (MQ=255)

                                                                                                                                         gaTCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTAtt    >  1:2648753/1‑140 (MQ=255)

                                                                                                                                         gaTCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTAtt    >  1:3568786/1‑140 (MQ=255)

                                                                                                                                           tCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGTAACTTGCGGAAAATAATTGTGGTATCATAGAATTTTGTCAAAATAATTTTATTGACATCGTCTTATTATCGTAGATATAATTTAAATTTTAtttt  >  2:1245931/1‑139 (MQ=255)

TGGAACGAATCAATGTATGGGAAGTGCTCCGAAATACGCTGACAAGAGAGAAAGGGCTTGGAGGTATTGAAACAAGAGGAGTGCTGAGAATTGGAATGCCTTATAAGTCTAATTAACAGTTGAAAACCTGTATAGGAGATCTATGCGGGTTTTTTATTTTACATAATGATACATAATTTACCGAAACTTGCGGAAAATAATTGAGGAATCATAGAATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTG  >  NZ_CP034943/52124‑52402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`