Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,531 A→C 11.6% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5310AC11.6% 600.0 / 9.6 268Q22H (CAA→CACEO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (162/56);  new base C (4/42);  total (167/101)
Fisher's exact test for biased strand distribution p-value = 4.26e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

ACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTG  >  NZ_CP034943/755393‑755661
                                                                                                                                           |                                                                                                                                  
tcttgTTTTTTCTAGGGAAGAGCTGTCCATTATTTATGCCATATGAAACGAAAAAAACGAGGTGTAATTAGAAATGGCAAATAACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAg                                                                                                                                   <  2:2611286/136‑1 (MQ=255)
aCCTGTTTTTTCAAGTTAAGTGCTGTCTATGATTGTTGCCATATGAAACTAAAAAAACGAGGTGGAATGAGAATTGGCAAATCACATTTTTCTTGCTGTCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAg                                                                                                                                   <  2:1849276/140‑1 (MQ=255)
     ttttttCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgggggg                                                                                                                              >  1:201107/1‑140 (MQ=255)
       ttttCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAACTGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACTTATTGAGACAGCACAAATCAAGGGGGGTg                                                                                                                            >  1:3768988/1‑140 (MQ=255)
           catgtgtagagCTGTCAATGATTGTTTAAATATTAAACTAAAAAAACGAGGGTGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTTTGGAGACAGCACAAATCAAGGGGGGTggggg                                                                                                                        <  2:3717485/133‑1 (MQ=255)
                        ttacATGATTGATGACATATTAAACGAAAAAAACGAGGGGGAATGATAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCt                                                                                                           <  2:8660/137‑1 (MQ=255)
                        gTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCCAATCACATTTATCTTGCTGGCGATTCAACTGTTCAACCGTCTGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCt                                                                                                           <  2:1360930/140‑1 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGTGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                          >  2:209535/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                          >  2:3227909/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGAGTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                          >  1:4545682/1‑140 (MQ=255)
                         tCCATGATTGATCCCAGATGAAACGAATAAATCGAGGGGGAATGAGAAATGGCAACTCACAGTGATCTTGCTGGCGATTCAACTGTTCAAACCTATGGAGACAGCACACATCAAGGGGGGGGTGGGCAGTGTCTCGGCTc                                                                                                          >  2:2174231/1‑140 (MQ=255)
                             ttatttatGCAATATTAAACGAAAAAAACGAGGGTGAATGAGAAATCTCAAATAACATTTATCTTGCTGGCGATTCAACAGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGCGCAGTTTCTCGGCTCGCAt                                                                                                      <  2:606281/138‑1 (MQ=255)
                             ctattgatGCCATATGAACCGACAAAAACGAGGGGGATTGAGAAATTGCTCATCACATTTTTCTTGCTGGCGATTAAACTGTTCAAACGTCTGGAGAGAGCACACATCAAGGGGGGTGGGGGCAGTGTCTCGGCTCGCAt                                                                                                      <  2:4631346/138‑1 (MQ=255)
                              gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCAta                                                                                                     >  1:1760330/1‑139 (MQ=255)
                              gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                     >  1:2434029/1‑140 (MQ=255)
                              gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                     >  1:3722040/1‑140 (MQ=255)
                                 ttattccATATTAAACGAAAAAAACGCTGTGGAATGAGAATTGGAAAATCAGATTTATCTTGATGGCGATTCAAGTCTTCAAACGTATTGAGACTGCACAACTCAAGGGGGGTCGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                  <  2:3150176/135‑1 (MQ=255)
                                 ttattcaatatGAAACGAAAAAAAAGATTGTGAATGAGAAATGGCAAATCAGATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                  <  2:2534268/133‑1 (MQ=255)
                                 ttatGCCTTATGAAACGACAAAAACGATGGGGAATGATAAATTTCACATCACTTTTATCTTTCTGGCGATTCAACTGTTCAAACCTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                  <  2:4535603/138‑1 (MQ=255)
                                 ttatGACATATGAAACGAAAAAAACGAGGTGGAATTAGAAATGGCAAATCACATTTTTCTTGCTGGCGATTCCACTGTTCAAACGTAGGGAGACAGCACCACTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                  <  2:1763232/138‑1 (MQ=255)
                                 tgatgaCATATTAAACGAAAACACCGTGGGTTAATGAGAACTGCCAAATCACATTTATCGTGCTGGCGATTCAACTGTTCAAACGTAGGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                  <  2:3513984/140‑1 (MQ=255)
                                   attccttatTAAACTAAAAAAAAGAGTGGTAATGACAAATGGCAAATCACATTTTTCTTGCTGGCTATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                <  2:3574313/134‑1 (MQ=255)
                                   atGCCATATGAAACGAACAAAAACGATGGGGACTGAGAAATGGCAAATCACAGTTATCTTGCTGGCGAATCAACTGTTCAAACGTATTGAGACAGAACAAATCAAGGGGGGTGGGGGCAGGTTCTCGGGTCGCAGCTGcc                                                                                                 >  1:3619356/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGCGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  2:3671000/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGTGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACTTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:4753899/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCCGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:4095283/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCCGTTTCTCGGCTCGCATCTGCCg                                                                                                >  2:3987463/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  2:1017304/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:693299/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  2:906575/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:1379920/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:2907430/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:2947204/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:322443/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  1:1812986/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATCGAGACAGCACAAATCAAGGGGGGTGGGGGCCGTTTCTCGGCTCGCATCTGCCg                                                                                                >  2:193053/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGGTCAAACGTATGGAGACAGCACAAATCATGGGGGGTGGGTGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                >  2:2312949/1‑140 (MQ=255)
                                     gacATATTAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGTATATTCAACTGTTCAAACTTATGGATACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                              <  2:1337238/138‑1 (MQ=255)
                                     gCCATATGAAACGAAAAAAACGCTGGGTAATGAGAACTGGCAAATCACCTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                              <  2:1303237/140‑1 (MQ=255)
                                     gCCATATGAAACGAAAAAAACGAGGGGGAATGACAAATGGCAACTCACATTTATCTTGCTGGCGATACAACGGTTCAACCGTATGCAGACAGCACAAATCAAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                              <  1:1258110/140‑1 (MQ=255)
                                     cacATATTAAACGAAAAAAAATACGGGTAATCATAAATGTCAAATCACATTTATCTTGCTGGCTATGCACCTGTTCAAACTTATGGATACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                              <  2:2637511/138‑1 (MQ=255)
                                       cATATTAAACGAAAAAAACGATGGTTAATGAGAAATGTCAAATCACATTTTTCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGc                                                                                            <  2:3777434/140‑1 (MQ=255)
                                       cATATGAAACGAAAAACACGAGGGGGAATGAGAAAGTGCAAATCACTTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATTGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGc                                                                                            <  2:800385/140‑1 (MQ=255)
                                         tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCGCGGCTCGCATCTGCCGGAGCat                                                                                          >  1:3032450/1‑140 (MQ=255)
                                          atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                         >  2:1756041/1‑140 (MQ=255)
                                             acaCGCAAAAAACGAGGGGGAATGAGAAATCGCAAATCACATTTATCTTGCTGGCGATACAACTGTTCAACCGTCTGGAGACAGCACAAATCACGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                      <  1:4069959/138‑1 (MQ=255)
                                             aaaaGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                      <  2:3086277/140‑1 (MQ=255)
                                             aaaCTAAATATATGAGGGGGTATTAGAAATGGCAAATCAGTTTTTTCTTGCTGGCGATTAATCTGTTCAAACGGATTGAGACAGCACAAATCATGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                      <  2:1660922/140‑1 (MQ=255)
                                             aaaCGAAAAAAACGAGGGGGAAGGAGAACTGGCAAATCACATTTATCTGGCTGGCGATTCAACTGGTCAACCGTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                      <  1:3505784/140‑1 (MQ=255)
                                              aaCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCa                                                                                     >  2:3813107/1‑140 (MQ=255)
                                               actaaaaaaaCGAGTGGGAATTATAAATGTCAAATCAAATTTTTCTTGTTTGAGATTCAACTGTTCAAACGAATGGATACAGCACAAATCACGGCGCGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                    <  2:1055061/137‑1 (MQ=255)
                                               accaaaaaaaCGAGGGGGAATGACCAATGCCAAATCACATTTATCTTGCGGCCGATACAACTGACCAAACCTAGGGACACAGCACAAACCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                    <  1:2176327/137‑1 (MQ=255)
                                               aCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                    <  2:4949436/140‑1 (MQ=255)
                                                  aaaaaaaaGAGGGGGAATGAGATATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTg                                                                                 <  2:1285002/140‑1 (MQ=255)
                                                  aaaaaaaCTAGGGGGAATTAGAAATGGCATATCTAATTTATCTTGCTGGCGATTCAACTGTTCAACCTTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTg                                                                                 <  2:4288800/140‑1 (MQ=255)
                                                   aaaaaaCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGa                                                                                >  1:2484079/1‑140 (MQ=255)
                                                        aaCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTaa                                                                            <  2:449350/140‑1 (MQ=255)
                                                                           tGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaaga                                                         >  1:4912111/1‑140 (MQ=255)
                                                                            ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGAAATCGGGGgaataa                                                        >  2:4359319/1‑140 (MQ=255)
                                                                              cAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGc                                                      >  2:4614236/1‑140 (MQ=255)
                                                                                aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTc                                                    >  2:3371624/1‑140 (MQ=255)
                                                                                   cacaTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGaa                                                 >  2:2787061/1‑140 (MQ=255)
                                                                                                cTGTCGATTCAACTTTTCACACGTATGGAGACAGCTCAAATCAAGGGGTGTGGGGGCAGTTTCTCGGCTCGCATATGCCTGATCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                    <  2:3010790/140‑1 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGTGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                    >  2:2777953/1‑140 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                    >  1:976734/1‑140 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                    >  2:4086947/1‑140 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGTCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAag                                   >  1:3239770/1‑140 (MQ=255)
                                                                                                     gATTCAACTGTTCACACGGCTGGAGACAGCACACATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAggg                               <  1:445299/140‑1 (MQ=255)
                                                                                                       ttCAAATGTTCAAACGTATCGCGCCACCACAACTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCa                             <  2:4023594/140‑1 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCTGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                     >  1:2668739/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                     >  1:1907997/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                     >  1:507982/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGACTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGTGAAGAAGCTCGAAATCATTTGTGGAAGAGGGCAGGCTTCAg                     >  2:2337921/1‑140 (MQ=255)
                                                                                                                 cccaCGTCTGGCGACCCCACAAATCCCGCGCGCTGGCGGCCGTGTCTCGGATCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                   <  1:763909/137‑1 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGACGAGGGCAGGCTTCAGGc                   >  1:2061448/1‑140 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                   >  1:3009463/1‑140 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                   >  1:606902/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg           >  1:4315024/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg           >  1:1075162/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg           >  1:4165193/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCACTCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg           >  2:4295543/1‑140 (MQ=255)
                                                                                                                         aggAGACAGCACAAATCAACGGGGCTCGGGGCAGTTTCACGGCTCACATCTGCCGGAGCATATTCAAGTGATTAACAGATCAATCGGGCGAAGAAGCACCAAAACAGTTGTGGAAGAGCGCAGGCATCAGGCGATTCtat           >  2:2681695/2‑138 (MQ=255)
                                                                                                                           gagaCAGCACAAATCATGGCGGGTGGGGGCAGTTTCTCGGCTCGCATGTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAt         <  2:404738/140‑1 (MQ=255)
                                                                                                                              aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg      >  2:4679353/1‑140 (MQ=255)
                                                                                                                                accacacaTCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCTTCTGCCGGAGCTTATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    <  2:2294337/138‑1 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGACGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    >  2:3487195/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    >  1:2310261/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    >  1:1444088/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    >  2:1468793/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    >  2:738600/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    >  2:557618/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTGTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGACAACCTTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt    >  1:2560257/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGATCATATTCAAGTCATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTTATTg  >  2:518078/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                    <  1:1307675/58‑1 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAtgg  >  2:4539247/1‑138 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg  >  1:1513034/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg  >  2:482752/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg  >  2:4743278/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg  >  2:3353030/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTAGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg  >  1:4359816/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                  
ACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTG  >  NZ_CP034943/755393‑755661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: