Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 828,787 G→A 100% E77K (GAA→AAA)  treC → alpha,alpha‑phosphotrehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943828,7870GA99.4% 1153.2 / ‑3.0 314E77K (GAA→AAA) treCalpha,alpha‑phosphotrehalase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (133/179);  total (133/181)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 316 total aligned reads displayed.

GCGATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGA  >  NZ_CP034943/828650‑828923
                                                                                                                                         |                                                                                                                                        
gCGATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTaaa                                                                                                                                        >  1:4633172/1‑140 (MQ=255)
  gATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATa                                                                                                                                      <  1:1615990/140‑1 (MQ=255)
     cTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACgg                                                                                                                                   >  2:2057584/1‑140 (MQ=255)
     cTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACgg                                                                                                                                   >  2:1612316/1‑140 (MQ=255)
       gaaggtcATTATTGAAAAGCTTGATTACTTAAAACCACTTCAGGTAGATGTGCTTTGGCTGACAGCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGa                                                                                                                                 <  1:3906687/134‑1 (MQ=255)
       gAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGa                                                                                                                                 <  1:4430862/140‑1 (MQ=255)
       gAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGa                                                                                                                                 <  1:1581585/140‑1 (MQ=255)
             cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           >  2:2342375/1‑140 (MQ=255)
             cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           <  1:4075231/140‑1 (MQ=255)
             cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           >  2:3354954/1‑140 (MQ=255)
             cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           <  1:3060145/140‑1 (MQ=255)
             cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           >  2:1982599/1‑140 (MQ=255)
             aatgattGAAAAGCTTTATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGCTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                           <  2:4858143/136‑1 (MQ=255)
              attattGAAAATCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAACTACGGGACGATgga                                                                                                                          <  2:2035080/140‑1 (MQ=255)
              attattGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgga                                                                                                                          <  2:3046019/140‑1 (MQ=255)
                tattGAAAAGCTTGATTACTTAAAAACCCGTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATggagg                                                                                                                        <  1:2878828/140‑1 (MQ=255)
                       aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGa                                                                                                                 >  1:2014815/1‑140 (MQ=255)
                       aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGa                                                                                                                 >  1:3577758/1‑140 (MQ=255)
                       aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGa                                                                                                                 >  2:3545155/1‑140 (MQ=255)
                        aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  1:3464742/140‑1 (MQ=255)
                        aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                                <  2:690968/140‑1 (MQ=255)
                            tGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGt                                                                                                            >  2:1244541/1‑140 (MQ=255)
                            tGATTACTTAAAAACACTTCAGGTAGATGTGCCTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGt                                                                                                            >  1:1933215/1‑140 (MQ=255)
                             gATTACTTAAAAACGCTACAGGTGGATGTGCTTTGGCTGACGCCGATTTATGATTCTCCCCAGCATGATAATGGGTACGATATTCGTGATTATTACTCGATTTATCCTGAATACGGAACGATGGAGGATTTTGAGCggct                                                                                                           <  2:2782066/140‑3 (MQ=255)
                             gATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGtt                                                                                                           <  2:4049513/140‑1 (MQ=255)
                              atgacatAAAAACACTTCAGGTAGATGTGCGTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  2:2788341/134‑1 (MQ=255)
                              aTTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  1:2157890/140‑1 (MQ=255)
                              aTTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  1:4283379/140‑1 (MQ=255)
                              aTTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  1:626774/140‑1 (MQ=255)
                                     aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCGGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                   <  1:1982599/140‑1 (MQ=255)
                                     aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                   <  1:2746920/140‑1 (MQ=255)
                                        aaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAg                                                                                                <  2:4749148/140‑1 (MQ=255)
                                            cTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCa                                                                                            <  1:3329121/140‑1 (MQ=255)
                                             ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                           >  2:2135132/1‑140 (MQ=255)
                                                 ggTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATaaaa                                                                                       <  2:3187901/140‑1 (MQ=255)
                                                   tAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAaga                                                                                     <  2:3408854/140‑1 (MQ=255)
                                                     gATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAgg                                                                                   >  2:4329385/1‑140 (MQ=255)
                                                       tgtgCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGATGcc                                                                                 >  2:4809241/1‑140 (MQ=255)
                                                       tgtgCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGcc                                                                                 >  2:4535038/1‑140 (MQ=255)
                                                           cTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGaa                                                                             >  2:3580081/1‑140 (MQ=255)
                                                              tGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgt                                                                          <  1:4514344/140‑1 (MQ=255)
                                                              tGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgt                                                                          <  1:2342375/140‑1 (MQ=255)
                                                                 cTGACACCGATTGATGATTCTCCGCAGCCTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAACTACGGGACGATGGAGGATTTTGAGCGGGTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgtcgt                                                                       <  2:1633619/140‑1 (MQ=255)
                                                                 cTGACACCGAGTTCTGATGCTCCGCAGCATGATAATGGGTAGGACATTCGTGATTATGCCTCGATTTATCCTAAATCCGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAATAGAGGCCTGAAAgtcgt                                                                       <  2:4090397/140‑1 (MQ=255)
                                                                    acacCGATTTATGATTCTCCGCAGCATGATAAGGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCAt                                                                    <  2:2901575/140‑1 (MQ=255)
                                                                         gATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAACTACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATc                                                               <  2:2729483/140‑1 (MQ=255)
                                                                         gATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATc                                                               <  2:346714/140‑1 (MQ=255)
                                                                          aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGAGTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCt                                                              >  1:334626/1‑140 (MQ=255)
                                                                              aTGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtc                                                          <  1:4929784/140‑1 (MQ=255)
                                                                              aTGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtc                                                          <  1:3367786/140‑1 (MQ=255)
                                                                              aTGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtc                                                          <  1:2502500/140‑1 (MQ=255)
                                                                                gATTCTCCGCAGCATGATAATGTGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:522197/140‑1 (MQ=255)
                                                                                gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  2:2519045/140‑1 (MQ=255)
                                                                                gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  1:2572985/140‑1 (MQ=255)
                                                                                 aTTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgtc                                                       <  1:1000594/140‑1 (MQ=255)
                                                                                    ctcCGCATCATGATAATGGGTATGACATTCGTGAGTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  2:568556/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGATGCTCATAAAAGAGGCCTGAAAGTCGTGATGGATCTTGTCGTCAAt                                                    <  2:4830587/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  2:1091266/140‑1 (MQ=255)
                                                                                    ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  1:1244541/140‑1 (MQ=255)
                                                                                    cgccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                    <  1:4156616/138‑1 (MQ=255)
                                                                                     tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          <  2:1760442/85‑1 (MQ=255)
                                                                                     tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                          >  1:1760442/1‑85 (MQ=255)
                                                                                     tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGACAGTCGTCATGGATCTTGTCGTCAATc                                                   >  2:4577261/1‑140 (MQ=255)
                                                                                     tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAta                                                   >  2:1383646/1‑139 (MQ=255)
                                                                                     tcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATc                                                   >  2:4165445/1‑140 (MQ=255)
                                                                                      ccGCAGCATGATAATGTGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  2:1518731/140‑1 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  2:4633172/140‑1 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  1:1075473/140‑1 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  1:2057584/140‑1 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  1:3398706/140‑1 (MQ=255)
                                                                                      ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                  <  2:1165157/140‑1 (MQ=255)
                                                                                       cGCAGCATGATAATGGTTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCAt                                                 >  2:2580860/1‑140 (MQ=255)
                                                                                            caTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAg                                                                           >  2:1279191/1‑109 (MQ=255)
                                                                                              tGATACTGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                          <  2:2251997/140‑1 (MQ=255)
                                                                                                   aTGGGTATGCGATTCGTGATTATGACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACagag                                     <  1:2803182/140‑1 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATGCATCAACAGAGcac                                  >  1:4746954/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:1512054/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:1974064/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:3413006/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  2:1912994/1‑140 (MQ=255)
                                                                                                      ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                  >  1:3165065/1‑140 (MQ=255)
                                                                                                        tATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                                <  1:97264/140‑1 (MQ=255)
                                                                                                           gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                             >  2:3895783/1‑140 (MQ=255)
                                                                                                            aCATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAAggg                            >  2:4778938/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGATGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGTTTTc                        >  1:1814299/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCAt                                                                    >  1:867634/1‑96 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                        <  2:4949729/108‑1 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  2:1356494/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  1:2226198/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  1:211044/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  1:1282450/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  1:3815154/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  1:711615/1‑140 (MQ=255)
                                                                                                                tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                        >  1:1668343/1‑140 (MQ=255)
                                                                                                                        attaCTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCg                >  2:1443994/1‑140 (MQ=255)
                                                                                                                            cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt            <  1:847263/140‑1 (MQ=255)
                                                                                                                                 tttATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCACGAGGCGATTTCGTCt       >  2:4443278/1‑140 (MQ=255)
                                                                                                                                 tttATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCt       >  1:1443434/1‑140 (MQ=255)
                                                                                                                                 tttATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCt       >  2:3760231/1‑140 (MQ=255)
                                                                                                                                      ccTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGa  <  2:840908/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GCGATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAGA  >  NZ_CP034943/828650‑828923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: