Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,035,865 G→T 9.8% C200F (TGC→TTC)  EO946_RS05350 → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,035,8650GT 9.8% 769.2 / 18.4 303C200F (TGC→TTC) EO946_RS05350ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (113/150);  new base T (38/2);  total (151/152)
Fisher's exact test for biased strand distribution p-value = 6.17e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

GATGAACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCAATGGAA  >  NZ_CP034943/1035726‑1036003
                                                                                                                                           |                                                                                                                                          
gatgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTg                                                                                                                                            <  1:2939053/140‑1 (MQ=255)
gatgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTg                                                                                                                                            <  1:4003861/140‑1 (MQ=255)
gatgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTg                                                                                                                                            <  1:2026474/140‑1 (MQ=255)
  tgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAACGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCa                                                                                                                                          <  2:1972478/140‑1 (MQ=255)
  tgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCa                                                                                                                                          <  1:1681190/140‑1 (MQ=255)
  tgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCa                                                                                                                                          <  2:4672065/140‑1 (MQ=255)
  tgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCa                                                                                                                                          <  2:2696817/140‑1 (MQ=255)
  tgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCa                                                                                                                                          <  2:3665561/140‑1 (MQ=255)
  tgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCa                                                                                                                                          <  2:167973/140‑1 (MQ=255)
  tgaACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCa                                                                                                                                          <  2:3515700/140‑1 (MQ=255)
    aaCCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATa                                                                                                                                        <  1:1273637/140‑1 (MQ=255)
    aaCCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATa                                                                                                                                        <  1:265995/140‑1 (MQ=255)
    aaCCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATa                                                                                                                                        <  1:3443574/140‑1 (MQ=255)
          ttAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAg                                                                                                                                  <  2:4141796/140‑1 (MQ=255)
              cGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGCGGAGCTAGGTGAGAACCTTTGCATACTGCAgaaag                                                                                                                              >  2:710804/1‑140 (MQ=255)
                 ccTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTGTTTTTTATTTTGTAGCCATAGGATGGTGCAGGTGGCGGTGCTATGTGAGAAACTTTTCCTACTGCCgcaaggaa                                                                                                                           >  1:1503486/1‑140 (MQ=255)
                 ccTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTTTTTTATTTTCTATCCATCGGATGGATCAGGTGGTGGAGCTATGTGAGAAACATTTCATACTTCAgaaaggag                                                                                                                           >  1:1134764/1‑139 (MQ=255)
                  cTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAgaaaggaaa                                                                                                                          <  2:2462318/140‑1 (MQ=255)
                       cccTGTCAATGTTGAGCTGTTAAAAGAGTCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCgg                                                                                                                     <  1:4910463/140‑1 (MQ=255)
                           gTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTGTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTTCATACTGCCGAAAGGAAAGCCGGTtgt                                                                                                                 >  2:3975274/1‑140 (MQ=255)
                           gTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTGTATTTTATTTTATAGCCATCGGATTGAGCATGTTGAGGATCTATTTGAGAACCATTTCATACTGCAGAAAGGTAATCCGGTtgt                                                                                                                 >  1:4682554/1‑140 (MQ=255)
                           gTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTGTATTTTATTTTATAGCCATCGGATTGAGCATGTGGAGGAGCGATGTGAGAACCATTGCATACTGCAGAAAGGAAACCCGGtttt                                                                                                                 >  1:1008049/1‑138 (MQ=255)
                           gTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTGGATTTTATTTTCTATCCCTCGGATGGAGCATGTGGAGGCGCTATGTTAGAAACTTTTGATACTGCATAAAGGAAAGCCCGTtgt                                                                                                                 >  1:3880092/1‑140 (MQ=255)
                           gTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCAGCGGATGGAGCATGTGGAGGAGCTATGTGAGAAACTTTGCATAATGCAGAAAGGAAAGCCGGTtgt                                                                                                                 >  1:3021391/1‑140 (MQ=255)
                           gTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTATAGCCAACGGATGGAGCATGTGGAGGAGCTATGTGATAACCTTTTCATACTGCAGAAAGGAAAACCGGtttt                                                                                                                 >  1:3286758/1‑138 (MQ=255)
                           gTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCGATTTTATTTTGTTTCCAACCGATTTAGCATTTGGATGAGCTATTTGATAAACTTTTCATAATTAAGAAAGGAAACCCGGtttt                                                                                                                 >  1:3409638/1‑138 (MQ=255)
                           gTCAATGTTGAGCGGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTATAGCCATCGGATGGAGCATGTGGAGGATCTATGTGAGAACCGTTGCATACTGCAGAAAGGAAAGCCGGtttt                                                                                                                 >  2:104174/1‑138 (MQ=255)
                            tCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTtgtg                                                                                                                <  1:3359671/140‑1 (MQ=255)
                                 gTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTgcaagg                                                                                                           <  1:1581555/140‑1 (MQ=255)
                                 gTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTgcaagg                                                                                                           <  1:1640752/140‑1 (MQ=255)
                                     aGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGATCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTgcaaggcaag                                                                                                       >  2:1072449/1‑140 (MQ=255)
                                     aGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTTCATACTTAAGAAAGTAAAACCGGTTGTgcaaggcaag                                                                                                       >  2:419261/1‑140 (MQ=255)
                                     aGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTTCATAATGCAGAAAGGAAAGCAGGGTGTgcaatgcaag                                                                                                       >  2:1893270/1‑140 (MQ=255)
                                     aGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTgcaaggcaag                                                                                                       <  2:2849069/140‑1 (MQ=255)
                                     aGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTgcaaggcaag                                                                                                       >  2:3963524/1‑140 (MQ=255)
                                     aGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTGTCTATTTTATTTTGTATCCATAGGATGGAGCATGTGGGGGAGCTATGTGAGAAACATTTCATACTGCACAAAGGAAAGCCGGTTGTgcaaggcaac                                                                                                       >  1:210885/1‑139 (MQ=255)
                                            aaaaGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAgg                                                                                                <  2:3771356/140‑1 (MQ=255)
                                               agagGCTGTGATTTCTTTGAAAATCTTCTTCGTTTGTATTTTATTTTCTAGACATCGTATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAACGAAAGCCGGTTGTGCAAGGCAAGCTGAAGgaga                                                                                             <  1:3200523/140‑1 (MQ=255)
                                                 agGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATa                                                                                                                                        >  2:408176/1‑95 (MQ=255)
                                                 agGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATa                                                                                           <  2:3562489/140‑1 (MQ=255)
                                                 agGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATa                                                                                           <  1:2749232/140‑1 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGTTTGTATTTTATTTTGTAGCCATCGGATGGAGCAAGTGGAGGAGCTATGTTAGAACCTTTTCATACTTCAGTAAGTTAAGCAGGTTTTGCAATGCAAGCTGAAGGATATAAAg                                                                                        >  2:3078258/1‑140 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGTTTGTATTTTATTTTCTAGCCATCGGATTGAGCATGTGGAGGAGCTATGTGAGAACCTTTTCATAATGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAg                                                                                        >  2:212136/1‑140 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCCTCGGATGGAGCATGTGGAGGAGCTATTTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCCAGCTGAAGGAGATAAAg                                                                                        >  2:4975122/1‑140 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATTTGGAGGAGCTATGTGAGAACCTTTGCATACTTCAGAAAGGAAAGCCGGTTGTGCAAGGCAACCTGAAGGAGATAAAg                                                                                        >  2:4276658/1‑140 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCGTTGCCTACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCGGAAGGAGATAAAg                                                                                        >  2:4340335/1‑140 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTATAGCCATCGGATGGAGCATGTGGAGGAGCGATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAg                                                                                        >  2:2352061/1‑140 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGTTTCGATTTTATTTTCTCGCCATCGGCTGGAGCATGTGGAGGAGCTATGTGAGAACCGTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCCAGCTGAAGGAGATAAAg                                                                                        >  2:4926823/1‑140 (MQ=255)
                                                    cTGTGATTTCTTTGAAAAACAGCGGCGGTTGTATTTTATTTTGTATCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCATTTCATACAGCAGAAATGAAACCCGGGTGTGCAAGGCAAGCAGAAGGAGATaaaa                                                                                        >  1:1201728/1‑139 (MQ=255)
                                                       tgATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTATAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGTAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGt                                                                                     >  2:4510798/1‑140 (MQ=255)
                                                                        aGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGaa                                                                    <  1:2320777/140‑1 (MQ=255)
                                                                        aGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGaa                                                                    <  1:1145832/140‑1 (MQ=255)
                                                                                  cTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCAtt                                                          <  1:3963524/140‑1 (MQ=255)
                                                                                  cTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCAtt                                                          <  2:3405490/140‑1 (MQ=255)
                                                                                  cTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCAtt                                                          <  1:4951367/140‑1 (MQ=255)
                                                                                  cTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCAtt                                                          <  1:2129371/140‑1 (MQ=255)
                                                                                       ttattttCTAGCCATCTGATGGAGCATGTTTAGGAGATATGTGAGAACCTTTGCATAATGCATAAAGGAATGGCGGTTGTGCAAGGCTAGCTGAAGTAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTc                                                     <  2:2993242/140‑1 (MQ=255)
                                                                                       ttattttCTAGCCATCGGATGGCGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAACGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTc                                                     <  2:4420764/140‑1 (MQ=255)
                                                                                       ttattttCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTc                                                     <  1:973312/140‑1 (MQ=255)
                                                                                       ttattttCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTc                                                     <  2:2193393/140‑1 (MQ=255)
                                                                                         attttCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCg                                                   <  1:3390101/140‑1 (MQ=255)
                                                                                                   cATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCt                                         <  2:3209811/140‑1 (MQ=255)
                                                                                                             aGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTg                               <  2:1931613/140‑1 (MQ=255)
                                                                                                             aGCATGTGGAGGAGATATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTg                               <  2:479613/140‑1 (MQ=255)
                                                                                                               cATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCa                             <  2:1196070/140‑1 (MQ=255)
                                                                                                                aTGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAg                            <  2:1326034/140‑1 (MQ=255)
                                                                                                                 tgtgGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATTATCTGCGTTTTTTGCAGt                           >  2:2576129/1‑140 (MQ=255)
                                                                                                                 tgtgGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGt                           <  1:104174/140‑1 (MQ=255)
                                                                                                                 tgtgGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGt                           <  1:2437307/140‑1 (MQ=255)
                                                                                                                 tgtgGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGt                           >  1:279293/1‑140 (MQ=255)
                                                                                                                  gtgGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCATGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGtc                          >  2:3416133/1‑140 (MQ=255)
                                                                                                                      aggagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCAt                      >  1:4100583/1‑140 (MQ=255)
                                                                                                                      aggagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCAt                      >  1:702754/1‑140 (MQ=255)
                                                                                                                       ggagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATg                     >  2:2422734/1‑140 (MQ=255)
                                                                                                                       ggagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATg                     >  1:373663/1‑140 (MQ=255)
                                                                                                                       ggagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATg                     >  1:433471/1‑140 (MQ=255)
                                                                                                                       ggagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGCCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATg                     >  2:2514243/1‑140 (MQ=255)
                                                                                                                        gagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTgcaaggcaag                                                                                                       <  1:3537357/57‑1 (MQ=255)
                                                                                                                        gagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGa                    >  2:769872/1‑140 (MQ=255)
                                                                                                                        gagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGa                    <  2:3548370/140‑1 (MQ=255)
                                                                                                                        gagCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGa                    <  2:1287628/140‑1 (MQ=255)
                                                                                                                         agCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATGTGCGTTTTTTGCAGTCTCATGaa                   >  2:4030637/1‑140 (MQ=255)
                                                                                                                             aTGTGAGAACCTTTGCATACTGCAGAAAGGTAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCa               <  1:3113673/140‑1 (MQ=255)
                                                                                                                             aTGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAATGCa               >  2:1492994/1‑140 (MQ=255)
                                                                                                                             aTGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCa               >  1:1667373/1‑140 (MQ=255)
                                                                                                                             aTGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCa               <  2:1582989/140‑1 (MQ=255)
                                                                                                                             aTGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCa               <  2:4502376/140‑1 (MQ=255)
                                                                                                                             aTGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCa               <  2:3286758/140‑1 (MQ=255)
                                                                                                                             aTGTGAGAACCTTTGCATACGGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGCATGTCACCAGTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCa               <  2:1517822/140‑1 (MQ=255)
                                                                                                                                    aaCCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCa        >  2:112109/1‑140 (MQ=255)
                                                                                                                                    aaCCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCa        >  2:1629261/1‑140 (MQ=255)
                                                                                                                                     aCCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGTAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCaa       <  1:4216484/140‑1 (MQ=255)
                                                                                                                                        tttGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCAtt                                                          >  1:2710147/1‑86 (MQ=255)
                                                                                                                                        tttGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGt                           <  2:557222/117‑1 (MQ=255)
                                                                                                                                        tttGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGt                           >  1:557222/1‑117 (MQ=255)
                                                                                                                                        tttGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCAATgg    <  1:4747167/140‑1 (MQ=255)
                                                                                                                                         ttGCATACTGAATAAAGGAAAGCCGGTTTTGCAAGGCATGCTTAAGGAGTTAAAGCGTTCTTTCGGCAAAAAGAATGTCACAATTCACTCCGTTGATGATTTGCGTTTTTTGCAGTTTCTTGAATGAATTCTCCAATgga   <  2:1772961/140‑1 (MQ=255)
                                                                                                                                          tGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCAATggaa  >  1:3611766/1‑140 (MQ=255)
                                                                                                                                          tGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCAATggaa  >  1:1298913/1‑140 (MQ=255)
                                                                                                                                          tGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCAATggaa  >  1:442222/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GATGAACCATTTAGCGGCCTTGACCCTGTCAATGTTGAGCTGTTAAAAGAGGCTGTGATTTCTTTGAAAAACAGCGGCGTTTCTATTTTATTTTCTAGCCATCGGATGGAGCATGTGGAGGAGCTATGTGAGAACCTTTGCATACTGCAGAAAGGAAAGCCGGTTGTGCAAGGCAAGCTGAAGGAGATAAAGCGTTCTTTCGGCAAAAAGAATGTCACCATTCACTCCGATGATGATCTGCGTTTTTTGCAGTCTCATGAAGGCATTCTCCAATGGAA  >  NZ_CP034943/1035726‑1036003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: