BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,040,035	G→C	6.0%	D429H (GAT→CAT)	EO946_RS05365 →	AAA family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,040,035	0	G	C	6.0%	908.2 / 2.8	356	D429H (GAT→CAT)	EO946_RS05365	AAA family ATPase
Reads supporting (aligned to +/- strand): ref base G (160/162); new base C (3/27); total (165/191)
Fisher's exact test for biased strand distribution p-value = 1.36e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 384 total aligned reads displayed.

GGTGCAGCAATACGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGT  >  NZ_CP034943/1039896‑1040173

ggTGCAGCAATACGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGaaagaaaag                                                                                                                                            >  1:1726606/1‑140 (MQ=255)

  tGCAGCAATACGTACAGGCAAGAGACCGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCAATCCTTGAAAACAACGAAAGAACAGAt                                                                                                                                          >  1:2911440/1‑140 (MQ=255)

  tGCAGCAATACGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGAt                                                                                                                                          >  1:639817/1‑140 (MQ=255)

          tACGTACAGGCAATAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGc                                                                                                                                  <  2:907072/140‑1 (MQ=255)

          tACGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGc                                                                                                                                  >  2:4575271/1‑140 (MQ=255)

           acttACAGGCAAGATAAATAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGATGGAAGACGCAGAAAAGGTCGTTCATGCCGCTACGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAACGAAGCg                                                                                                                                 <  2:1444257/137‑1 (MQ=255)

            cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCTTGCCGCTGCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                >  1:4698746/1‑140 (MQ=255)

            cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAGAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                >  1:1400817/1‑140 (MQ=255)

            cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                >  1:886887/1‑140 (MQ=255)

            cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                >  1:1534367/1‑140 (MQ=255)

            cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                >  2:1888572/1‑140 (MQ=255)

            cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                >  2:4707371/1‑140 (MQ=255)

            cGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCgg                                                                                                                                >  1:3967811/1‑140 (MQ=255)

              tACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGct                                                                                                                              >  1:3163844/1‑140 (MQ=255)

                 aGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCtt                                                                                                                           >  1:619419/1‑140 (MQ=255)

                 aGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCtt                                                                                                                           >  1:4257188/1‑140 (MQ=255)

                 aGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCtt                                                                                                                           >  1:4170913/1‑140 (MQ=255)

                 aGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCtt                                                                                                                           >  1:4041135/1‑140 (MQ=255)

                  ggCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGACGCGGCTCTTa                                                                                                                          <  2:2951329/140‑1 (MQ=255)

                  ggCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTa                                                                                                                          <  2:4132085/140‑1 (MQ=255)

                  ggCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAACAAAACATAAAGAAGCGGCTCTTa                                                                                                                          <  2:1788989/140‑1 (MQ=255)

                    cAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGCTAAAGACGCGGCTCTTaaa                                                                                                                        <  2:3947406/140‑1 (MQ=255)

                      agagACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCTTGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAATAAGCGGCTCTTAAAGc                                                                                                                      >  2:1191372/1‑140 (MQ=255)

                       gagcCAGAAACCGCCCTCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAACAAGCGGCTCTTAAAGCa                                                                                                                     <  2:2841822/140‑1 (MQ=255)

                       gagaCAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGTAAACAACGAAAGACAAGATCAAGAAGCGGCTCTTAAAGcc                                                                                                                     >  1:2757235/1‑139 (MQ=255)

                       gagaCAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATCAAGAAGCGGCTCTTAAAGCa                                                                                                                     <  2:3173344/140‑1 (MQ=255)

                       gagaCAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCa                                                                                                                     <  2:648916/140‑1 (MQ=255)

                        agaCAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGCGCTGGAAGACGAAG‑AACGGTCGTTCATGCCGCTTCGTCCCCTATCCTTGAAAACAACGAAACCAAACATAAACAAGCGGCTCTTAAAGCata                                                                                                                   <  1:1218494/140‑1 (MQ=255)

                        agaCAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCat                                                                                                                    >  2:4839946/1‑140 (MQ=255)

                         taCAGAAACCGCCAGCTTGATGAGACTTTTGAACTTCCCCGGCAAGAGCTGGAAGAGGAATAAAAGGTCGTTCATGCGGCTTCGTCCGATATCCTTTAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCata                                                                                                                   <  1:2220436/139‑1 (MQ=255)

                         taCAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCTTTCATGCCGCTTCGTCCGATATCCTTGAAAACAACGAAAGAAAAGATAAACAAGCGGCTCTTAAAGCata                                                                                                                   <  2:2205304/139‑1 (MQ=255)

                         caCAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGCCACGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGATATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCata                                                                                                                   <  1:2308601/139‑1 (MQ=255)

                               aaaCGGCAGCTTTTTGAGAAATTTGAACTTGCCCGGCAAGAGCTTTATGACGCATAAATGGTCTTTCTTCCCGGTTCGTCCGCTATCATTTAAAACAACGAAAGAAAACATAAACAAGCGGCTCTTAAAGCATAtgatga                                                                                                             <  1:2466157/140‑1 (MQ=255)

                               aaaCGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAACGAAGCGGCTCTTAAAGCATAtgatga                                                                                                             <  2:2398395/140‑1 (MQ=255)

                                   ggCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAACGGTCGTTCATGCCGCTTCGTCCGCTATCCTTTAAAACAACGAAAGAAAAGATAACCAAGCGGCTCTTAAAGCATATGATGAAGca                                                                                                         <  1:2071413/140‑1 (MQ=255)

                                        ctggatgaGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAACGGGCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGACAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAggg                                                                                                    <  2:2316529/137‑1 (MQ=255)

                                        cTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAggg                                                                                                    <  2:214394/140‑1 (MQ=255)

                                        cTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAggg                                                                                                    >  2:1858863/1‑140 (MQ=255)

                                        cTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCATTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAggg                                                                                                    <  2:2070453/140‑1 (MQ=255)

                                        cTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGCTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAggg                                                                                                    <  2:536115/140‑1 (MQ=255)

                                         ttGATGAGACATTTGATCTTGCTGGGATAGAGCTGTAAGACGTATAAAAGTTCGTTAATGGCTCTTCGTCCTCTATCCTTTAAAACAACGAAACAAAAGTTAACGAATCGGCTATTAAAGCATATGATGAAGCACAgggg                                                                                                   <  2:2717267/140‑1 (MQ=255)

                                         ttGATGAGACATTTGAACTTGCCCGGCAAGAGCTGTAAGACGCATAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAAAAACGAAACAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAgggg                                                                                                   <  2:1985986/140‑1 (MQ=255)

                                         ttGATGAGACATTTCAACTTGCCCGGCAAGAGCTGGAACACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGATATCCTTCAAAACAACGAAAGAAAAGATACACACGCGGCTCTTAAAGCATATGATGAAGCACAgggg                                                                                                   <  1:861583/140‑1 (MQ=255)

                                          tgatgaGACATTTGACCTTGCCCGGCACGAGCTGTATGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGATATCCTTGAAAACAACGAAACAACAGATAAACAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                  <  1:1060363/140‑1 (MQ=255)

                                          tgatgaGACATTTGAACTTGCCCGGCAAGAGCGGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAACAAAAGACAAACAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                  <  1:1806104/140‑1 (MQ=255)

                                          tgatgaGACATTGCAACTTGCCCGGCCAGAGCTGGAAGACGCAGAAACGGTCGTTCATGCCGCTTCGTCCGCTATCCTTTAAAACAACGAACGAAAAGATAAACACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGc                                                                                                  <  1:2363847/140‑1 (MQ=255)

                                             tgaGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGt                                                                                               >  1:4308269/1‑140 (MQ=255)

                                                   aTTTTAACTTCCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCTTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAgg                                                                                         <  1:1302563/140‑1 (MQ=255)

                                                      tccaCTTGCCCGGAAAGAGGTGTAAGACGAAGAAACGGTCGTTCCTGCCGCTTCGTCCGCTATCCTTGAAAACACCGAAACAAAACATAAACACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGc                                                                                      <  1:4111981/137‑1 (MQ=255)

                                                          ctggCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAACGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGc                                                                                  <  2:3553636/137‑1 (MQ=255)

                                                          cTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGATATCCTTGAAAACAACGAAAGAAAAGATAACGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGc                                                                                  <  2:4266534/140‑1 (MQ=255)

                                                             gCCCGGCAAGAGCTGGAAGACGCAGTAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGAATATGATGAAGCAGAGGGGCAGTATCAGGAGCAAGCGaa                                                                               >  2:1231771/1‑140 (MQ=255)

                                                             gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGaa                                                                               >  1:1906004/1‑140 (MQ=255)

                                                             gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGaa                                                                               >  1:2646487/1‑140 (MQ=255)

                                                             gCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGaa                                                                               >  1:1508427/1‑140 (MQ=255)

                                                                  gCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTga                                                                          >  2:1681798/1‑140 (MQ=255)

                                                                  gCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTga                                                                          >  2:2453750/1‑140 (MQ=255)

                                                                  gCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCAGATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTga                                                                          >  2:3739404/1‑140 (MQ=255)

                                                                       agCTGGAAGACGCATAAAAGGTCTTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAACAAAAGATAAAGAAGCGGCTCTAAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGagagag                                                                     <  2:26653/140‑1 (MQ=255)

                                                                       agCTGGAAGACGCAGAAATGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAACGAAAGGAGAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCACGCGAAGCGGagcgag                                                                     >  2:4526293/1‑140 (MQ=255)

                                                                       agCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGagagag                                                                     <  2:1803235/140‑1 (MQ=255)

                                                                                 cgcgGAAAAGGCCGTTCGTGCCGCTTCGTCCGCGATTCTTGAAAACAGCCAAAGAAAAGATAAAGAAGCAGCTCTTAGAGCATATGATGAAACACAGGGGCAGCACCAAGAGCAAGCGAAGCTCAGAGAACAGCtaacgt                                                           >  2:3612072/1‑135 (MQ=255)

                                                                                 cGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGt                                                           >  1:713240/1‑140 (MQ=255)

                                                                                 cGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGt                                                           >  1:3017221/1‑140 (MQ=255)

                                                                                 cGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGt                                                           >  2:4109597/1‑140 (MQ=255)

                                                                                 cGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGt                                                           >  2:809683/1‑140 (MQ=255)

                                                                                  gCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGtt                                                          <  2:1726606/140‑1 (MQ=255)

                                                                                          ggTCGTTCATGCCGCTTCGTCCGATATCCTTGAAAACAACGAAAGAAAAGATAACGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGaaa                                                  <  1:3103863/140‑1 (MQ=255)

                                                                                           gTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAgggg                                                                                                   <  2:1585422/90‑1 (MQ=255)

                                                                                           gTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAg                                                 >  1:4463213/1‑140 (MQ=255)

                                                                                           gTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAg                                                 >  1:1845569/1‑140 (MQ=255)

                                                                                              gTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGgca                                              <  2:640092/140‑1 (MQ=255)

                                                                                              gTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGgca                                              >  2:4043004/1‑140 (MQ=255)

                                                                                              gTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAACAAAAGATAAAGACGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGgca                                              <  1:2403710/140‑1 (MQ=255)

                                                                                                tcttGCCGCTTCGACCGGTATCCTTTAAAACAACTAAATAACATATAAAGCCCCGGCTCTTATAGCATATGATGAAGAACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGgcagc                                            <  2:1927965/137‑1 (MQ=255)

                                                                                                        cTTCGTCCGCTATCCTTGAAAACAACGAAACAAAAGATAAACAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTaaa                                    <  2:49407/140‑1 (MQ=255)

                                                                                                          tCGTCCGCTATCCTTGAAAACAACGACAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGTTGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCCGCAGGCTAAACa                                  <  2:3027613/140‑1 (MQ=255)

                                                                                                             tCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGaa                               >  1:3518978/1‑140 (MQ=255)

                                                                                                                 cTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAg                           <  2:1400585/140‑1 (MQ=255)

                                                                                                                  tacccTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAgg                          >  2:877178/4‑140 (MQ=255)

                                                                                                                      cTTGAAAACAACGAAAGAAAATATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGt                      <  2:4622364/140‑1 (MQ=255)

                                                                                                                         gAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCAt                   >  2:148894/1‑140 (MQ=255)

                                                                                                                         gAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCAt                   <  2:1091524/140‑1 (MQ=255)

                                                                                                                           aaaCAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCACGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTg                 <  2:1330706/140‑1 (MQ=255)

                                                                                                                           aaaCAACGAAAGAAAACATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTg                 <  1:4808540/140‑1 (MQ=255)

                                                                                                                            aacaacGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                <  1:3335735/140‑1 (MQ=255)

                                                                                                                            aacaacGAAACAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                <  1:1781533/140‑1 (MQ=255)

                                                                                                                            aacaacGAAACAAAACATCCAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                <  1:11216/140‑1 (MQ=255)

                                                                                                                            aacaacCAAAGAAAACATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                <  1:809014/140‑1 (MQ=255)

                                                                                                                            aacaacCAAACAAAAGATAACCAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGc                <  1:4735418/140‑1 (MQ=255)

                                                                                                                              caacGAAAGAAACGATAACGAAGCGGCTCTTAAAGCATATGCTGAAGCACAGGGGCAGTATCAGGAGCAAGCGACGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACCGAAAAAGGTTGTCATTGCAg              <  2:4189815/140‑1 (MQ=255)

                                                                                                                               cacGAAACAAAACATACAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGc             <  2:105445/139‑1 (MQ=255)

                                                                                                                                   aaccaaaagaGCACGACGCGGCTCTTAAAGCATCTGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt         <  1:162221/136‑1 (MQ=255)

                                                                                                                                   aaagaaaacaTAAAGACGCGGCTCTTAACGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt         <  2:3849035/140‑1 (MQ=255)

                                                                                                                                   aaagaaaacaTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt         <  1:371393/140‑1 (MQ=255)

                                                                                                                                   aaacaaaagaTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGt         <  1:3865731/140‑1 (MQ=255)

                                                                                                                                         aagaTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTAtgttg   >  2:4399648/1‑140 (MQ=255)

                                                                                                                                         aagaTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTAtgttg   >  2:3798371/1‑140 (MQ=255)

                                                                                                                                         aagaTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAACCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTAtgttg   >  2:3354137/1‑140 (MQ=255)

                                                                                                                                          agaTAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTAtgttgt  <  2:525037/140‑1 (MQ=255)

GGTGCAGCAATACGTACAGGCAAGAGACAGAAAACGGCAGCTTGATGAGACATTTGAACTTGCCCGGCAAGAGCTGGAAGACGCAGAAAAGGTCGTTCATGCCGCTTCGTCCGCTATCCTTGAAAACAACGAAAGAAAAGATAAAGAAGCGGCTCTTAAAGCATATGATGAAGCACAGGGGCAGTATCAGGAGCAAGCGAAGCTGAGAGAGCAGCTGAGGTTTTTTGAAAGGCAGCAGGCTAAACAGAAAAAGGTTGTCATTGCAGCTGGTATGTTGT  >  NZ_CP034943/1039896‑1040173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`