Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,111,643 C→A 9.6% L580I (CTA→ATA)  addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,111,6430CA 9.6% 536.9 / 5.7 237L580I (CTA→ATA) addBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base C (39/148);  new base A (32/2);  total (87/150)
Fisher's exact test for biased strand distribution p-value = 1.51e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

AGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGA  >  NZ_CP034943/1111509‑1111782
                                                                                                                                      |                                                                                                                                            
     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                                    <  2:4958640/140‑1 (MQ=255)
     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                                    <  2:4176333/140‑1 (MQ=255)
       tCAGCAGGCGTGGGATGCAGTCATTCAGCTAGTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGccgg                                                                                                                                  >  2:4576013/1‑138 (MQ=255)
          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                              <  2:215934/140‑1 (MQ=255)
          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                              <  1:2964323/140‑1 (MQ=255)
          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAATTTTGTGTAATTACGCCGgcgc                                                                                                                              >  2:1899044/1‑140 (MQ=255)
          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAAGGATGGAAGCCGGCGCGGAGTCGCGTTAATGTTCTCTACTTACGccgtggg                                                                                                                               >  1:4899885/1‑135 (MQ=255)
               cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTCTAATTACGCCGGCGCGTGAc                                                                                                                         >  2:117589/1‑140 (MQ=255)
               cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATATAATTCAGCCGGCGCTTTAc                                                                                                                         >  2:2696182/1‑140 (MQ=255)
               cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACAATTTGTCTAATTAACCCTGCGCtgtac                                                                                                                         >  1:3739378/1‑136 (MQ=255)
                gTGGGATGGAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAcc                                                                                                                        <  1:68433/140‑1 (MQ=255)
                gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGCGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAcc                                                                                                                        <  1:2284971/140‑1 (MQ=255)
                gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAATTTTATATAAATACGCCTGCGCTTGaac                                                                                                                        >  2:1588116/1‑138 (MQ=255)
                gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAcc                                                                                                                        <  1:458648/140‑1 (MQ=255)
                gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAcc                                                                                                                        <  1:1773330/140‑1 (MQ=255)
                   ggATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAgg                                                                                                                     <  2:431652/140‑1 (MQ=255)
                   ggATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAgg                                                                                                                     <  2:1055519/140‑1 (MQ=255)
                    gATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTATCTAATTCCGCCGGCGCGTGACCACgt                                                                                                                    >  2:1343465/1‑140 (MQ=255)
                        cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGCTGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTCTAAGTCCGCCTGCGCGTGACCAGGTGgtt                                                                                                                >  2:3419864/1‑140 (MQ=255)
                        cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTGAACTTTTGTCTTATTACGCCTGGCGCTGGGCAGGTGttt                                                                                                                >  1:2980329/1‑140 (MQ=255)
                        cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAATTTTGTCGAATTAAGCCAGCGCGTGAACCGGTGttt                                                                                                                >  1:2347294/1‑140 (MQ=255)
                        cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTGTAAAAATACGCCTGCCCGTGTCCCGGGGttt                                                                                                                >  1:4409320/1‑140 (MQ=255)
                         aGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAATTTTATCGAATTACGCCTGCGCGTGACCACGTGGTTg                                                                                                               >  1:3280208/1‑140 (MQ=255)
                          gTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGt                                                                                                              <  2:3297354/140‑1 (MQ=255)
                          gTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGt                                                                                                              <  2:4736282/140‑1 (MQ=255)
                           tCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAAATTTATCTAATTCCCCCGGGGCTTGTCCAGgggtgtttc                                                                                                             >  2:2420772/1‑135 (MQ=255)
                             aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                           <  2:3825445/140‑1 (MQ=255)
                             aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTGAGTTTTTCTAATTACGCCAGCGGGTGTCCACGGGttttttgg                                                                                                           >  1:1882447/1‑135 (MQ=255)
                              ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTCTCTAAATACGCCGGCGCTTGACCAGGTGTTTGTAGGc                                                                                                          >  2:2050958/1‑140 (MQ=255)
                              ttCAGCTACTTGAGGAGTGTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTATGTTCTTACCCCTGCGCGTGAGCCGGGGGTTGTgggg                                                                                                          >  1:3142314/1‑139 (MQ=255)
                                    tACTTGAGGAGTTTGTTGAAATGATGGGGGATGCTGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATg                                                                                                    <  1:4887674/140‑1 (MQ=255)
                                     aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTTTATAATTACGCCTGGGCTTTTCCACGTGTTTTTGGGCAATATgg                                                                                                   >  1:3589604/1‑140 (MQ=255)
                                     aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAATTTTGTATAAATGCGCCGGCGCGTGGACACGGGTTTTTGGGCaaaaatg                                                                                                   >  1:4221266/1‑135 (MQ=255)
                                      cTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATCTAATTACGCCTGCGCTTGACCCCGGGTTTGTCGGCAATATTGa                                                                                                  >  2:263437/1‑140 (MQ=255)
                                       ttGAGGAGTTTGTTGAAATGATGGGTGATGATGCGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCGTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                 <  2:855323/140‑1 (MQ=255)
                                       ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                 <  2:4074052/140‑1 (MQ=255)
                                       ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                 <  2:3989426/140‑1 (MQ=255)
                                       ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTGTATAATTAAGCCTGCGCTTTAAAAGGTGTTTTTAGGGAataatgat                                                                                                 >  2:3833517/1‑135 (MQ=255)
                                            gagTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATGTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATc                                                                                            <  2:2234731/140‑1 (MQ=255)
                                              gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                          <  2:3606347/140‑1 (MQ=255)
                                                 tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAATTTTTTCTAATTGCGCCTGCGCGTTTCCAGGTGGTTTTGGGGACTATTGATTTATTCaaaa                                                                                       >  1:4038608/1‑140 (MQ=255)
                                                 tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATATAATTACACCATCGCTTGACCACGGGTTTTTGGGCAAAATGGATTTTTACAGaa                                                                                       >  2:2964422/1‑140 (MQ=255)
                                                 tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTATAAAAATACCCCAGCCCTTGACAACGTGTTTTTCGGCAATATTGATTTaaacacaa                                                                                       >  2:3178096/1‑133 (MQ=255)
                                                 tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAAATTTGTCTAATTACGCCAGCGCGTGACCACGTGTTTTTGGGCAATATTGATTTATACACaa                                                                                       >  1:4873037/1‑140 (MQ=255)
                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                               <  2:3882730/140‑1 (MQ=255)
                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                               <  2:413792/140‑1 (MQ=255)
                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                               <  2:508391/140‑1 (MQ=255)
                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                               <  1:858541/140‑1 (MQ=255)
                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                               <  1:3448359/140‑1 (MQ=255)
                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCGGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                               <  1:385928/140‑1 (MQ=255)
                                                         tgatgGGGGATGATGAGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                               <  2:1269304/140‑1 (MQ=255)
                                                          gttgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                              <  2:185607/138‑1 (MQ=255)
                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                              <  1:534487/140‑1 (MQ=255)
                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                              <  1:3627381/140‑1 (MQ=255)
                                                          gatgGGGGATGATGAGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                              <  1:2389766/140‑1 (MQ=255)
                                                          gatgGGGGAGGATGAGATTTCTCTTGATTTGTTTCAGCATATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTCTCTACTTCCGCCTGCGCTTGACCCGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                              <  1:3627321/140‑1 (MQ=255)
                                                             gggggATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTGTATAAATGCGCCTTCGCTTGAACAGGTGTTTGTGGGGAATATTGATTTATACAGAATGTACGGCAAct                                                                           >  2:1816198/1‑140 (MQ=255)
                                                                 gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                       <  1:322496/140‑1 (MQ=255)
                                                                 gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                       <  2:1626113/140‑1 (MQ=255)
                                                                    gatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTCTCTAAATAACCCTGCGCTTGACCACGTGTTTTTAGGCAAAATGGATTTATACAAAAAGTAAGGAACCACATGCAc                                                                    >  2:2571807/1‑140 (MQ=255)
                                                                    gatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTCTAGTATTAAGCCCTCGCCTTACCCGGGGTTTGTGGGCCAAAATGATATATAAAGAAAGTAACGCACCGCGTGCAc                                                                    >  2:4544593/1‑140 (MQ=255)
                                                                     atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTATATAAATAACCCAGCGCTTGAAAAGGTGTTTTTGGGGAAAATTGATTTATACATAATGTAAGGCACCACTTGGAcc                                                                   >  2:83518/1‑140 (MQ=255)
                                                                     atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTTTAGAAATACCCCATCGCTTGTCCAGGTGTTTGTCGGCAAAATGGAATTATACACAATGTAAGGCACCTCTTTCAca                                                                   >  2:3697159/1‑139 (MQ=255)
                                                                     atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATATAATTAAGCCTGCGCTTTACCAGGGGGTTTTCGGCAAAATTTTTTTTTCAAAAATGTACGGCACCACTTGCAcc                                                                   >  2:3383732/1‑140 (MQ=255)
                                                                      tgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTACTTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCt                                                                  <  2:2335947/140‑1 (MQ=255)
                                                                       gagaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCtt                                                                 <  1:258556/140‑1 (MQ=255)
                                                                          aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAAATTTATCTAATTACCCCGGCGCTTGACCAGGGGTTTTTGGGCAATATGGATTTATACAGAAATTAACGCCCCACTTGGACCGTtgt                                                              >  2:3200116/1‑140 (MQ=255)
                                                                               tctTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTGTATAAATTCGCCGGCGCGTGACCCGGTGGGTTTGGGGCATATTGATTTTTACAGAAATTAAGGCACCCCGTGGACCATTGtggtgg                                                         >  1:414362/1‑140 (MQ=255)
                                                                                      ttGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCaaa                                                  <  1:462866/140‑1 (MQ=255)
                                                                                         tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacga                                               <  2:1550694/140‑1 (MQ=255)
                                                                                          ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                              <  1:1109053/140‑1 (MQ=255)
                                                                                          ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                              <  1:1657516/140‑1 (MQ=255)
                                                                                          ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                              <  1:4366211/140‑1 (MQ=255)
                                                                                          ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                              <  1:4295765/140‑1 (MQ=255)
                                                                                          ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGAAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                              <  1:1955520/140‑1 (MQ=255)
                                                                                                caaTGATGGAAGCCGGCGCGGAGTCGCTGACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATAGGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGtt                                        <  2:3431780/139‑1 (MQ=255)
                                                                                                         aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                               <  1:2571807/140‑1 (MQ=255)
                                                                                                         aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                               <  1:4717365/140‑1 (MQ=255)
                                                                                                         aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                               <  1:1588116/140‑1 (MQ=255)
                                                                                                         aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAAGATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                               <  1:1348093/140‑1 (MQ=255)
                                                                                                                       tCGCTTACATTTTCTATAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaaa                 <  1:1695245/140‑1 (MQ=255)
                                                                                                                          cTTACATTTTATATAATTACGCCTGCGCTTGACCAGGTGTTTGTCGGGAAAATGTATTTATACCGAAATTACGGCACCGCTTGCACCGTTGTGGTGGGGGGAAAAGACGGCGTTCTGCCTGCACGCCCGGATGTAAATgg              >  2:2793219/1‑140 (MQ=255)
                                                                                                                           ttACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGTATTTATCCAGAATTTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg             <  1:2965016/140‑1 (MQ=255)
                                                                                                                           ttACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg             <  1:2958956/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCGCCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:1510368/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  2:576978/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:1281520/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:608846/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:1028592/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:3372523/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:2812559/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATAGGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:2143998/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  2:3589604/140‑1 (MQ=255)
                                                                                                                              cATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  2:3012093/140‑1 (MQ=255)
                                                                                                                              aaTTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTGGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg          <  1:1163325/139‑1 (MQ=255)
                                                                                                                                 tttCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTTCACCGTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTg       >  1:4697762/1‑140 (MQ=255)
                                                                                                                                   tctcTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTc     >  1:3389172/1‑140 (MQ=255)
                                                                                                                                   tctcTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCGGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTc     >  1:2708247/1‑140 (MQ=255)
                                                                                                                                   tctcTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAAGATGGATTTATCCAGAATGTACGGCACCTCTTGCACCGTGGTGCTGGCGGCAAACGACGGCGTTCTGCCTGCACGCCCTGAGGAAAATGGGGTGCTGTc     >  1:2506953/1‑140 (MQ=255)
                                                                                                                                     tctAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGACAATGGGGTGCTGTCgg   >  1:2075997/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                            
AGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGA  >  NZ_CP034943/1111509‑1111782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: