Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,263,953 G→T 9.7% I77I (ATC→ATA fetB ← iron export ABC transporter permease subunit FetB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,263,9530GT 9.7% 614.6 / 17.5 241I77I (ATC→ATAfetBiron export ABC transporter permease subunit FetB
Reads supporting (aligned to +/- strand):  ref base G (114/95);  new base T (2/30);  total (116/125)
Fisher's exact test for biased strand distribution p-value = 7.12e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

TTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATC  >  NZ_CP034943/1263814‑1264091
                                                                                                                                           |                                                                                                                                          
        aTCGGAATGACATATCGGGCCGTAAGCGTGATGATATGAAGAGACATCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTACAAAACGAACCGATTCTATTTTTCTTTAGTTTGATAACAtt                                                                                                                                    >  2:3800037/1‑140 (MQ=255)
        aTCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtt                                                                                                                                    >  2:4635261/1‑140 (MQ=255)
        aTCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGAGAACACCCTCCAAAACGAACCGATTGTATTTTTGTTTCGTTTGATAACAtt                                                                                                                                    >  1:685010/1‑140 (MQ=255)
             aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                               <  1:3199255/140‑1 (MQ=255)
             aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                               <  1:996178/140‑1 (MQ=255)
                  cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCTTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                                          >  1:2512105/1‑140 (MQ=255)
                  cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                                          <  1:4635261/140‑1 (MQ=255)
                  cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                                          >  1:4346192/1‑140 (MQ=255)
                      tCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTg                                                                                                                      <  2:4920816/140‑1 (MQ=255)
                      tCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTg                                                                                                                      <  2:4892261/140‑1 (MQ=255)
                      tCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAAGCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTg                                                                                                                      <  2:1818513/140‑1 (MQ=255)
                      tCGGGACGTATGCGTGATGATATTAAGAGACATCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACTCGGCGAACACCCTCCAAAACTAACCGATTTTTTTTTTTTTTATTTTTATAACATTTTGCGGTGCTACtt                                                                                                                      <  1:3832170/140‑2 (MQ=255)
                       cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                     >  2:1510409/1‑140 (MQ=255)
                       cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                     >  2:1412685/1‑140 (MQ=255)
                       cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                     >  2:3530699/1‑140 (MQ=255)
                        tggCCGTAAGCGTGATGATATGAAGAGAGAGCAGAAGTCCCTGCGTAACAATTTCTACACTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                                    <  2:2800072/139‑1 (MQ=255)
                        gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                                    <  2:2347487/140‑1 (MQ=255)
                        gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                                    <  1:797555/140‑1 (MQ=255)
                        gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                                    <  1:4493716/140‑1 (MQ=255)
                        gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                                    <  1:2346280/140‑1 (MQ=255)
                        gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                                    <  1:285787/140‑1 (MQ=255)
                         ggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCa                                                                                                                   <  2:2052252/140‑1 (MQ=255)
                          gCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCaa                                                                                                                  >  1:3439074/1‑140 (MQ=255)
                          gCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCaa                                                                                                                  >  1:260354/1‑140 (MQ=255)
                           ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAAc                                                                                                                 <  1:3892184/140‑1 (MQ=255)
                           ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAAc                                                                                                                 <  2:2513965/140‑1 (MQ=255)
                             gTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACAt                                                                                                               >  2:705270/1‑140 (MQ=255)
                              tAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGGTTGATAACATTTTGCGCTGCTACTGCCAACAtt                                                                                                              >  2:3496264/1‑140 (MQ=255)
                                   ggtatgatATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTACCGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAg                                                                                                         <  2:2657047/137‑1 (MQ=255)
                                   gggATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAg                                                                                                         <  1:4939727/140‑1 (MQ=255)
                                     gatgatATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCa                                                                                                       >  1:1982880/1‑140 (MQ=255)
                                       tgatATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCAcc                                                                                                     <  2:4695808/140‑1 (MQ=255)
                                           atGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCa                                                                                                 >  2:3447892/1‑140 (MQ=255)
                                             gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                               <  2:4316296/140‑1 (MQ=255)
                                             gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                               <  1:3394829/140‑1 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                               >  2:3537203/1‑102 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTCCTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                         >  1:1339281/1‑140 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                         >  1:4821105/1‑140 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                         >  2:250684/1‑140 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                         >  1:2316812/1‑140 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTGTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                         >  1:2398069/1‑140 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAATATCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTTCGCTGCTACTGACAACATTAACAGCACCATAAGCAAGATa                                                                                         >  2:1337701/1‑140 (MQ=255)
                                                   aCAGCAGAATTCCCTGCGTCACAAGTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                         >  2:4358796/1‑140 (MQ=255)
                                                    cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                               <  2:3724120/101‑1 (MQ=255)
                                                    cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                               >  1:3724120/1‑101 (MQ=255)
                                                    cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaa                                                                                        >  1:2104228/1‑140 (MQ=255)
                                                      gcagAATTCCCTGCGTCACAATTTCTACAATGGCTAACGCGGCGTACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTGTGATAACATTTTGCGCTGCTCGTGCCACCCTTAGCAGCACCATCTGCACGagacaa                                                                                      >  2:3470920/1‑135 (MQ=255)
                                                       cagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaaa                                                                                     <  1:4135201/140‑1 (MQ=255)
                                                          aaTTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAg                                                                                  >  2:2312750/1‑140 (MQ=255)
                                                          aaTTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAg                                                                                  >  2:1887185/1‑140 (MQ=255)
                                                            ttCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGAt                                                                                          <  2:4976593/130‑1 (MQ=255)
                                                            ttCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGAt                                                                                          <  2:3230693/130‑1 (MQ=255)
                                                               ccTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATgg                                                                             >  1:786735/1‑140 (MQ=255)
                                                                 tGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGtc                                                                           >  1:115682/1‑140 (MQ=255)
                                                                 tGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGtc                                                                           >  2:4582105/1‑140 (MQ=255)
                                                                  gCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGtct                                                                          >  2:3073606/1‑140 (MQ=255)
                                                                         attttCTACAATCGCTAACGCGGCTAACACCCTCCAAAACGCACCTTTTGTATTTTTGTTTCTTTTTATAACATTTTGCGGTGCTACTGCAAACATTAACCGAACCATCAGCAATATAAAAACAGGATGGTCTCCGCTaa                                                                   <  1:278851/138‑1 (MQ=255)
                                                                                   tgcGCTACCGGGGCTAACCCCCTCCAAACCGAACGGATTGTATTTTTCTGTCTTTTGATAACATTTTGCGCTGCTACGGCCAACATTAACCGCACCATCAGCAAGATAAAAACAGGATGCTCTCCGCGAAAAAAACGCGa                                                         <  2:1728544/138‑1 (MQ=255)
                                                                                   attgCTAACGCGGCTAAAACACTCCAACGCGAACCTTTTGTATTTTTCTTTCGTTTTATAACATTTTGCGCTGCTACTGCCAACATTAACTGCACCATCAGCAATATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                         <  2:2129862/137‑1 (MQ=255)
                                                                                   atggatAAGGCGGCTAACACCCTCAAAAACCAACTGTTTTTATTTTTGTTTCTTTTTATAACTTTTTGCGCGGATACTGCAAACTTTAACACCACCAACAGAAATATAAAAACTGTATGTTCTCCGCGAAAAATAAGCGa                                                         <  1:3487216/135‑1 (MQ=255)
                                                                                   atccatAACGCGGCTAACACCCTCCAAAACGAACCGTTTGTATTTTTCTTTCTTTTTATAACATTTGGCGCTGCTACTGCAAAAATTAACAGCACCATCAGCAATATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                         <  2:3114184/135‑1 (MQ=255)
                                                                                   aTCGCTACCGCGGCCAACACCCACAAAAACGAACGTTTTTTTTTTTTATTTCTTTTGTTAAATTTTTGCGCTGCTAATGCAAAAATTAACAGCACCAACAGAAATATAAAAACAGGTTGTTCTCCGCGAAAAATAAGCGa                                                         <  1:4911561/140‑1 (MQ=255)
                                                                                   aTCGCTAACGGGGCGAACACCCTCCAAACCGACCCGATTTTATTTTTATTTCGTTTGATAACTTTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                         <  2:3662879/140‑1 (MQ=255)
                                                                                   aTCGCTAACGCGGCTAACACCCTCAAAAACGAACCGTTTTTATTTTTCTTTCGTTTTATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAATATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                         <  2:3444351/140‑1 (MQ=255)
                                                                                   aTCGCTAACGCGGCGAACACCCTCCAAAACTAACCGTTTGTATTTTTATTGTGTTTTATAAAATTTTGCGCTGCTACTGCCAACATTAAAAGAACCATCAGAAATATAAAAACAGGAGGGTCTCCGGGAAAAATAAGCGa                                                         <  2:878191/140‑1 (MQ=255)
                                                                                   aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCgc                                                         >  2:314353/1‑139 (MQ=255)
                                                                                   aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                         >  2:299788/1‑140 (MQ=255)
                                                                                   aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                         >  1:514135/1‑140 (MQ=255)
                                                                                   aTCGCTAACGCGCCGAACACCCTCCAAAACCACCCTTTTGTATTTTTCTTTCGTTTGATAAAATTTTGCGCTGATACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGa                                                         <  2:3414799/140‑1 (MQ=255)
                                                                                   aTCGATAACGCGGCGAAAACCCTCCAAAACCAACAGTTTTTTTTTTACTTTCGTTTGATACCTTTTTGCGCTGCTACTCACAACATTAACAGCACCATCAGCAAGATAAAAACAGTTTGGTCTCCGCGAAAAATAAGCGa                                                         <  2:1665848/140‑1 (MQ=255)
                                                                                     ggCTACCGGGGCTAACACCCTCAAAAATGAACCTTTTTTATTTTTATTTGGTTTTATAAATTTGTGGTGTGTTACTGCCAAAATTACCAGCACCAACAGAAATATAAAAACCGTTTGGTCTCCGCGAAAAATAAGCGACa                                                       <  1:3530699/139‑1 (MQ=255)
                                                                                     cGCTACCGCGGCGAACCCCCTCCAAAACGACCCGATTGTATTTTTCTTGCGTTTGATAACATTTTGCGCTGATACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACa                                                       <  2:2883877/140‑1 (MQ=255)
                                                                                     cGCTAACGGGGCGAACACCCTCCAACACCAACCGATTGTATTTTTCTTTCGTTTTATAACTTTTTGCGCTGCTACTGCCAAAATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGACa                                                       <  2:1240512/140‑1 (MQ=255)
                                                                                     cGCTAACGCGGCGAACACCCTCCAAACCGACCGGATTGTATTTTTCTTTCTTTTTATAACATTTTGCGCTGCTAGTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGAGGGTCTCCGCGAAAAATAAGCGACa                                                       <  2:3255043/140‑1 (MQ=255)
                                                                                     cGCTAACGCGGCGAACACCCTCCAAAACGAACCTTTTGTATTTTTCTTTCTTTTGATAACATTTTGCGCTGCTACTGCAAAATTTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACa                                                       <  2:1107332/140‑1 (MQ=255)
                                                                                     cGCTAACGCGGCGAACACCCTCCAAAACCAACCGATTTTATTTTTCTTTCGTTTGATACCATTTTGCGCTGCTACTGCCAACATTAACAGCACAATCAGAAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACa                                                       <  2:937247/140‑1 (MQ=255)
                                                                                       cTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGt                                                     >  1:3105494/1‑140 (MQ=255)
                                                                                         aaCGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTAc                                                   >  2:69360/1‑140 (MQ=255)
                                                                                           cgcgGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACAt                                                 >  2:222903/1‑140 (MQ=255)
                                                                                              ggcgACCACCCTCCAAAAAGAACCGCTTGTATTTTTCTTTCGTTGGATAACATTTTGCGCTGCTCCGGCCAACATTAACAGCACCATCAGCAAGATAAAAGCAGGATGGTCTCCGCGAACAATAAGCGACAGTACATAAc                                              >  2:390115/1‑140 (MQ=255)
                                                                                              ggcgAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAAc                                              >  2:4652560/1‑140 (MQ=255)
                                                                                                cgAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCg                                            >  2:2429248/1‑140 (MQ=255)
                                                                                                      cccTCCAAAACGAACCGTTTGTATTTTTATTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAAACGCACCATCAGCAAGATAAAAACCGGATGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATa                                      <  2:4736086/140‑1 (MQ=255)
                                                                                                      cccACCAAACCGAACCGATTTTATTTTACTTTCGTTTGATAACATTGTGCGCTGCTACTGCAAACTTTAACACCACCATAACCAAGATAAAAACCGGAGGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                                      <  2:2025786/140‑1 (MQ=255)
                                                                                                      cacaaaaaaaCTAACATATTTTATTTTTATTTCTTTTGATAACATTTTGCGCTGATACTGCCAAAATTAACAGAACAATCAGAAATATAAAAACAGTTTGTTCTCCGCGAAAAATACGCGACAGTACATAACCGATGATa                                      <  1:4670821/134‑1 (MQ=255)
                                                                                                      cacTCAAAACCTAACCTTTTTTATTTTTTTTTCGTTTTATAACATTTTGCGCTGCTACTGCCAACATTAAAACAACCATCAGAAATATAAAAACAGGTTGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                                      <  2:2589834/138‑1 (MQ=255)
                                                                                                         tCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                                   >  1:2805177/1‑140 (MQ=255)
                                                                                                         cccAAACCCAACCTATTGTATTTTTGTTTCGTTTGATAAAATTTTGCGCTGCTACGCCCAACATTAACACCACCATCACAAAGAAAAAAACAGGATGGTCTCCGCCAAAAATACGCGACAGTACATAACCGATGATAagc                                   <  1:4059211/139‑1 (MQ=255)
                                                                                                         cacAAACCGAACCGATTTTTTTTTTCTTTGTTTTGATAAAATTTGGCGCTGCTACTGCAAACATTAACACCACCATCCCCAAGAAAAAAACTGGCTGGTCACCGCCAAAAATACGCGACAGTACATAACCGATGATAagc                                   <  1:2254130/138‑1 (MQ=255)
                                                                                                          ccAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagca                                  >  1:1848201/1‑140 (MQ=255)
                                                                                                          ccAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagca                                  >  1:4277378/1‑140 (MQ=255)
                                                                                                                  aaCCGATTGTATTTTTCTTTCGTTGGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTAcc                          >  1:1523797/1‑140 (MQ=255)
                                                                                                                   ccctaTTGTATTTTTATTTCGTTTTATAACATTTTGCGCTGCTACTGCAAACATTAACAGCACCATCAGAAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                         <  2:1319324/136‑1 (MQ=255)
                                                                                                                   aCCTATTTTATTTTTCTTTCGTTTTATAACATTTTGCGCTGATACTGCAAAAATTAACAGCACCATCAGCAAGATAAAAACCGGATGGTATCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                         <  2:3917334/140‑1 (MQ=255)
                                                                                                                   aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                         >  1:2543953/1‑140 (MQ=255)
                                                                                                                   aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                         >  1:1954856/1‑140 (MQ=255)
                                                                                                                    ccGATTGTATTTTACTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCAGCAGCAAGATAAAAACAGGATGGTCTCCGCGAAACACAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGc                        <  2:1380345/140‑1 (MQ=255)
                                                                                                                        ttGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATACGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGcc                    <  2:4462213/140‑1 (MQ=255)
                                                                                                                                    ttcTTTTTATAAAATTTTGCGCTGCTACTGCCAAAATTAACACCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCa        <  2:4257782/140‑1 (MQ=255)
                                                                                                                                          tGATAACTTTTTGCGCTGATACTGCCAACATTAACACCACCATCAGAAATATAAAAACTGTAGGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATc  <  1:1896198/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATC  >  NZ_CP034943/1263814‑1264091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: