Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,591 A→T 9.2% intergenic (+269/‑35) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,5910AT 9.2% 1257.8 / 18.4 434intergenic (+269/‑35)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (93/285);  new base T (38/3);  total (141/293)
Fisher's exact test for biased strand distribution p-value = 4.42e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 436 total aligned reads displayed.

ATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATG  >  NZ_CP034943/112454‑112728
                                                                                                                                         |                                                                                                                                         
      aTTCAAAGGCGGCCGCCGCCGTTACAGTCTGAAGTAGGGAGAGCAGAATTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTATCTGTCATGTTTTCTTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGaaa                                                                                                                                   <  2:2768944/140‑1 (MQ=255)
               cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATaaaaaaaa                                                                                                                          >  2:2527829/1‑136 (MQ=255)
               cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATaga                                                                                                                          >  1:3431831/1‑140 (MQ=255)
               cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACAGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATata                                                                                                                          >  2:4445932/1‑138 (MQ=255)
                ggcCGCCGCCGTTACAGGCTGAAGTTGGGATAGCAGTAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                         <  2:726890/140‑1 (MQ=255)
                ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                         <  2:3431831/140‑1 (MQ=255)
                 gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTGGGTGAAGAGGAAAGAAAATAGAGaa                                                                                                                       >  1:1793257/1‑140 (MQ=255)
                  ccgccgccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGaa                                                                                                                       <  2:3697907/140‑1 (MQ=255)
                  ccgccgccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGaa                                                                                                                       <  2:2027629/140‑1 (MQ=255)
                      agccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAAAAAAATAGAGAAGTgg                                                                                                                   >  1:2119686/2‑140 (MQ=255)
                       gccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTGGGTGTAGAGGAAATAAAATATAGAAGTggg                                                                                                                  >  1:1428589/1‑140 (MQ=255)
                       gccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGGTTAAGCGTCTCTGTCATGTTTTCCTGCAGAGGCGCGTTTGTTTTTGGGGGAATTGGAAAGAAAAGAGAGCAGTgcg                                                                                                                  >  1:247174/1‑138 (MQ=255)
                        ccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCTGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAAGAGGAAATCAAATAGAGAAGTgggg                                                                                                                 <  2:1539999/140‑1 (MQ=255)
                        ccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTgggg                                                                                                                 <  2:1863623/140‑1 (MQ=255)
                          gTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTgggggg                                                                                                               <  1:2911821/140‑1 (MQ=255)
                                   tGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAATAGCAAAACAAAAAGAAAATAGGGGGGGACCAagtg                                                                                                      >  1:674729/1‑140 (MQ=255)
                                     aaGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGGAGAGGAAAAAAAATAGACAAAGGGGGGGACGCCTGGGtt                                                                                                    >  1:2043244/1‑140 (MQ=255)
                                     aaGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCTTGTGtt                                                                                                    >  2:3323208/1‑140 (MQ=255)
                                           ggAGAGCAGAAGTCTGCTTTTCAAACCGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTat                                                                                              >  2:1659183/1‑139 (MQ=255)
                                           ggAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCCGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAAAAGATAAGTGGGGGGAAGCAGGTGTTTTTTAg                                                                                              >  1:791241/1‑140 (MQ=255)
                                            gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                             <  1:1704648/140‑1 (MQ=255)
                                            gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                             <  1:4133379/140‑1 (MQ=255)
                                               agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTGGGTGAAGACGAAATAAAATAGATAAGTGGGGGGAACCAAGTTTTTTTTAGAAtt                                                                                          >  1:1463142/1‑139 (MQ=255)
                                                 cAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGca                                                                                        >  2:1184933/1‑139 (MQ=255)
                                                 cAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGTCGCTTTTTTTATTGTGTGAAGAGGAAATCAAAAAATGAATTGGGGGGACTCATGTTTTTTTTATaaatga                                                                                        >  1:2446414/1‑136 (MQ=255)
                                                 cAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCAAGTGTTTTTTAGAATGCt                                                                                        >  2:4835228/1‑140 (MQ=255)
                                                      gTCTGCTTTTCAACCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                   <  1:1308162/140‑1 (MQ=255)
                                                      gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                   <  1:4939356/140‑1 (MQ=255)
                                                      gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTATCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                   <  1:3562586/140‑1 (MQ=255)
                                                        cTGGTTTTCACACAGAGTGGAACCGCGGGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                 <  1:2680495/140‑1 (MQ=255)
                                                        cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                 <  1:2247210/140‑1 (MQ=255)
                                                        cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                 <  2:1114054/140‑1 (MQ=255)
                                                        cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                 <  2:2242127/140‑1 (MQ=255)
                                                        cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                 <  1:2602939/140‑1 (MQ=255)
                                                         tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaag                                                                                <  1:557052/140‑1 (MQ=255)
                                                         tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTGGGTGTAGAGGTAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAaagaag                                                                                >  1:4870658/1‑140 (MQ=255)
                                                          gattttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:2964702/138‑1 (MQ=255)
                                                          gCTTTTCAAAGAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGGAGAGGCGCTATTTTTATTGGGTGAAGAGGACATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:398880/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCTCTGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:3209024/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:1406770/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:76504/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:4308369/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:2810041/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  2:3525677/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  1:2698778/140‑1 (MQ=255)
                                                          gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                               <  1:3806809/140‑1 (MQ=255)
                                                                  aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                       <  2:1457813/140‑1 (MQ=255)
                                                                  aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTGATTGGGTGAAGAGGAACTCAAATAGAGAAGTGGGGGGACGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                       <  1:4807234/140‑1 (MQ=255)
                                                                  aaCAGAGTGGAACCGCGCGGTTTAAGCGACTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                       <  1:4695803/140‑1 (MQ=255)
                                                                   aCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCGTGTGTTTTTTAGAATGCTCAAAGAAGACATTGATAc                                                                      <  1:51649/140‑1 (MQ=255)
                                                                   aCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATAc                                                                      <  1:1000383/140‑1 (MQ=255)
                                                                         tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                                <  2:4758259/140‑1 (MQ=255)
                                                                         tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                                <  2:1469245/140‑1 (MQ=255)
                                                                           gAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGTAGATGAAATAAAATATATAAGTTGGGGGAAGCAAGTTTTTTTTTTAATTCTAAAATAAAAAATTTATAAACTGttag                                                              >  1:4549309/1‑138 (MQ=255)
                                                                             aCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAt                                                            <  1:4386857/140‑1 (MQ=255)
                                                                                 cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTTTAGATGTAATAAAAAAGATTAGTGGGGGGGAGCCTTTGTTTTTTTTTATTCACAAAAAAGACAATTATTAACGGTTAGATCAAg                                                        >  1:2342690/1‑140 (MQ=255)
                                                                                 cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTATTGGAAAACAAATATAGACGTGGGGGGGAGCATTTGTTTTTTAGATAGCTCAAATAAGAAATTTATACAGTGTTCGATaaat                                                        >  1:2171096/1‑139 (MQ=255)
                                                                                 cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGaa                                                                                                                       <  1:4518185/77‑1 (MQ=255)
                                                                                 cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAg                                                        >  2:3044878/1‑140 (MQ=255)
                                                                                 cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAg                                                        >  2:241698/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGGGGCGCTTTTTTTATTGGGTGAAGCGGAAATCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGAAATTGATACAGGGTTCGATAAAGATCCAGCAGCaagaa                                         >  2:4936758/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGTATAGGAAATAAAATATAGAAGTGGGGGGAAGCATGTGTTTTTTATAAAGCTCAAATAACACAATGATAAACTGTTAGATAAATATCCAGCACCaaaaa                                         >  1:331202/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGGAGAGGACATCAAATAGAGAAGTGGGGGGAACCATGTGTTTTTTTTAATGCTCAAAGAAGAAAATTATAAAGTGTTCGATAAAAATACATCAGCaagaa                                         >  1:2804534/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGTATAGGTAATCAAAAATAGAAGTGGGGGGGAGCATGGGTTTTTTTTTATTCTCAAAGAAGACAATTATTAAATTTTCGATGAAAATACAGCACCaagaa                                         >  1:1803269/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAAAGGAAATCAAATAGAGAATTGGGGGGAATCAAGTGTTTTTTAGAATTCTAAAATAAGAAATTGTTAAAGTGTTAGAGCAAGATACAGCAGCaagaa                                         >  2:384213/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAAAGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATACAGCAGCaagaa                                         >  2:2556223/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGGTGATGTAAACAAATATAGTAGTGGGGGGAAGCATGTGTTTTTTTGAATGCTCAAATAATACATTGTTACAGTGTTCGATAAACATCAAGCAGCaagaa                                         >  1:1202718/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAATATGAAATGAAATAGAGAAGTGGGGGGAAGCAGGTGTTTTTTAGACTGCTCAAAGAAGAGATTGATAAAGTGTTCGCTCAAGATCCAGAAGCaataa                                         >  2:3779009/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGTGAAGTGGGGGGAAGCCTGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                         >  2:4662555/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAt                                                                                                         <  1:401683/76‑1 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAATACATTGATACAGTGTTAGATAAAGATCCAGCAGCaagaa                                         >  2:1812102/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAATACATTGATAAAGTGTTCGATCAAGATACAGCAGCaaaaa                                         >  2:2058934/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                         >  2:4095615/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                         >  2:2399221/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGGAAGCAAAAAGAGGAGTGGGGGGCAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTAGAGAAAGATCCAGCAGCaagaa                                         >  1:735630/1‑140 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTGGGGTAAGAGGAAATCAAATACAGAACTGGGGGGAAGCCGGTGTTTTTTATAATTCTCAAATAACACATTGATACAGTGTTAGAAAAAGATACACCAGCaagat                                         >  2:1703424/1‑139 (MQ=255)
                                                                                                tctGTCATGTTTTCATGCAGAGGCGCGGGGGTTTTTGTGTGACGTGGTAAGAGAATAGAGAAGGGGGGGGAAGGCTGGGTGTTTTAGAATGCTCCAGGTAGTCATTGTTACACTGGGCGGTCGAGAGCCCGCGCCaagaa                                         >  2:2491668/1‑140 (MQ=255)
                                                                                                         ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGTAATCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAAGACATTGATAAAGTGGTAGATCAAGATCCAGCAGCAAGAAGTTAttttt                                >  2:2001278/1‑139 (MQ=255)
                                                                                                           ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGATGAAATCAACTAGACCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                              <  2:3183852/140‑1 (MQ=255)
                                                                                                                 cAGAGGCGCTTTTTTTATTGGGTGTAGAGGAATTCAAATAGAGACGTGGGGTGATTCATGTGTTTTTTAGAATGCTCATAGAAGACATTGATACAGTGTTCGATCTAGATCCAGCAGCAAGAAGTTATTTTGAAGTTAtt                        <  1:15239/140‑1 (MQ=255)
                                                                                                                 cAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGAtt                        <  2:101712/140‑1 (MQ=255)
                                                                                                                      gcgcTTTTTTTATTGGGTGAAGAGGAACTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAAc                   <  2:1835051/140‑1 (MQ=255)
                                                                                                                          ttattttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGCGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTAt               <  2:4172806/137‑1 (MQ=255)
                                                                                                                                  ttGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGttt       <  1:3082101/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa      <  2:2134230/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa      <  2:2687513/140‑1 (MQ=255)
                                                                                                                                     ggggAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  1:3751742/137‑1 (MQ=255)
                                                                                                                                     ggTGTAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGAGCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  1:3696451/140‑1 (MQ=255)
                                                                                                                                     ggTGCAGAGGAACTCAAATAGACACGGGGGGGGCACCATGTGTTTTTTAGAATGCTCAACGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  2:3616500/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGATGAAATCAACTAGAGACGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  2:3740991/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGTAAATCAACTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  2:3935777/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCCAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  2:1077291/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGTCGTCGGGCGAAGCATGTGTTTTTTAGAATGCTAAAAGAAGACATTGATTCAGTGTTCGTTCAAGATCCAGCCGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  2:1271759/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  1:3162922/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  2:235736/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  1:2980936/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAAGCTAGAAGTCGGGGCAAGCATCTGTTTTTTAGAATGCTCAAACAAGACATTGATACACTGTTCGATCAAGATCCAGCAGCAAGAAGTTAATTTGAAGTTATTTTAACTTATTCCGGTTTACa    <  2:612120/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAAGCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa    <  1:3949910/140‑1 (MQ=255)
                                                                                                                                       tGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg  <  2:4486560/140‑1 (MQ=255)
                                                                                                                                        gAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCcag                                                  >  2:51276/1‑91 (MQ=255)
                                                                                                                                         |                                                                                                                                         
ATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATG  >  NZ_CP034943/112454‑112728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: