Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,514,854 T→G 9.1% intergenic (+58/+1) EO946_RS07915 → / ← EO946_RS07920 YlaH‑like family protein/YlaI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,514,8540TG 9.1% 492.8 / 5.1 207intergenic (+58/+1)EO946_RS07915/EO946_RS07920YlaH‑like family protein/YlaI family protein
Reads supporting (aligned to +/- strand):  ref base T (59/118);  new base G (26/3);  total (85/122)
Fisher's exact test for biased strand distribution p-value = 8.48e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TGATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTT  >  NZ_CP034943/1514717‑1514986
                                                                                                                                          |                                                                                                                                    
tGATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCttt                                                                                                                                    >  2:1248160/1‑140 (MQ=255)
       cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAtttttt                                                                                                                             >  1:4547693/1‑140 (MQ=255)
       cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAtttttt                                                                                                                             >  1:1230844/1‑140 (MQ=255)
       cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTGGTGTATGGTAAAGCGGCCGCTTTAtttttt                                                                                                                             >  1:4145065/1‑140 (MQ=255)
       cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCGTTGTTTGTGGTTTGTATGGTAAAGCGGCCGCGTTAtttttt                                                                                                                             >  2:2138198/1‑140 (MQ=255)
                 aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTGTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAGTTTTTGAatttttgt                                                                                                                   >  1:2881561/1‑138 (MQ=255)
                 aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACACAGCTGAGAAGCGGAGTCTTTTTTTGGGGTTTGTATGGTAAACCGGCCGCGGTATTTTTTGActttttgt                                                                                                                   >  1:3135961/1‑138 (MQ=255)
                         cATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTg                                                                                                           <  1:895129/140‑1 (MQ=255)
                           tttCCAACATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTATTGTGTTTTGTATGGTAAACCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCt                                                                                                         <  2:139624/140‑1 (MQ=255)
                           tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCt                                                                                                         >  2:800972/1‑140 (MQ=255)
                           tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCGGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCt                                                                                                         <  2:3047803/140‑1 (MQ=255)
                                      cGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGACGCTGAGTCTTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTGTTTTTTTTGCTGTCCCGGGTg                                                                                               >  1:2214947/1‑140 (MQ=255)
                                      cGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTcc                                                                                                     >  2:573199/1‑133 (MQ=255)
                                      cGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTcc                                                                                                     <  1:573199/133‑1 (MQ=255)
                                            tATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGACGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGTCTTTTTCTTTTTTTTTGTGTCCCGGGTGATAAGTg                                                                                        >  1:4808844/1‑140 (MQ=255)
                                                   cGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTc                                                                                 <  1:1710805/140‑1 (MQ=255)
                                                      aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGACGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCg                                                                              >  2:675914/1‑140 (MQ=255)
                                                      aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTCTGTCCCGGGTGAAAAGTGAATTTTCCCg                                                                              >  2:3138244/1‑140 (MQ=255)
                                                      aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTGTGTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTTACTTTTTGTTTTTTTTGGTGTCCCGGTTGTAAAGTTAATTTTACCg                                                                              >  2:2039777/1‑140 (MQ=255)
                                                           ggACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTTTTTGGCTTTTTGTTTTTTTTTGTTTTCCGGGTGAAAAGTGTATTTTACCGttttg                                                                         >  1:3802165/1‑139 (MQ=255)
                                                            gACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGAATTTTTGTTTTTTTTGGTGTGCCGGGTGAAAAGTTAATTTTACCGTTTTCa                                                                        >  1:3432598/1‑140 (MQ=255)
                                                                gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCg                                                                    <  1:800972/140‑1 (MQ=255)
                                                                   tGCAAAATCATAAAATGACTCCGCTGAGAAGCTGAGTCTTTGTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACGTTTTCTTTTTTTTGCTGGGCCGGGTGAAAAAAGAATATTGCCCTTTTCAGCCGttc                                                                 >  2:1909213/1‑140 (MQ=255)
                                                                   tGCAAAATCATAAAAAGACTCAGCTGAGACGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTGTTTTTTTTTCTGTCCCGGGTGAAAAGTGAATTTTACCGTTTTAAGCCGttg                                                                 >  2:232925/1‑139 (MQ=255)
                                                                      aaaaTCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGttctta                                                              <  1:3857120/140‑2 (MQ=255)
                                                                       aaaTCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTtcttct                                                             <  1:1569340/140‑1 (MQ=255)
                                                                       aaaTCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTtcttct                                                             <  1:1339647/140‑1 (MQ=255)
                                                                       aaaTCATAAAAAGACTAAGCTGAGAAGCTGAGTCTTTTTTTGTTTTTTGTATGGTAAAGGGGGTGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTtcttct                                                             <  1:24965/140‑1 (MQ=255)
                                                                           cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGTCTTTTTGTTTTTTTTTCTGTCCCGGGTGGAAAGTGAATTTTCCCGTTTTGAGCCGTTCGTGTGttt                                                         >  1:4400747/1‑140 (MQ=255)
                                                                           cATAAAAAGACTCAGCTGAGAAGCGGAGTCTTTTTTGGTGTGTTGTATGGTAAAGCGGCCGCGTTCTTTTTTGTCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGGGAATTTTCCCGTTTTCAGCCGTTCTTCTGttt                                                         >  2:2213088/1‑140 (MQ=255)
                                                                             tAAAAAGACTCAGCTGAGAAGCTGCGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATGTTTTGAGTTTTTGTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCGGGTCTTCTGttttt                                                       >  2:1105978/1‑140 (MQ=255)
                                                                             tAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTGTTTTTTTTTCTTTGGCGGGTGAAAAGTGAATTTTACCGTTTTACGCCGTTCTTGTGttttt                                                       >  1:517175/1‑140 (MQ=255)
                                                                             tAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGGGTTTTGTATGGTAAAGCGGCCGCGTTAGTTTTTGTATTTTTCTTTTTTTTGCTGTCCCGGGTGAAAACTGAATTTTACCCTTTTAAGCCGTTCGTGTGttttt                                                       >  1:3668930/1‑140 (MQ=255)
                                                                             tAAAAAGACTCAGCTGAGAAGCTGAGTCGTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTGTATTTTTTGACGTGTTGTTTTTTTTGGTGTCCCGGGTGAAAAGTGGATTTTCACGTTTTCGTCGGTTGTTGTGttttt                                                       >  1:1113152/1‑140 (MQ=255)
                                                                               aaaaGACTCAGCTGAGAAGCTGAGACTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTg                                                     <  1:1810722/140‑1 (MQ=255)
                                                                                 aaGACTCAGCTGAGAAGCTTAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                   <  1:3094930/140‑1 (MQ=255)
                                                                                 aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                   <  1:2813595/140‑1 (MQ=255)
                                                                                 aaGACTCAGCTGAGAAGCTGAGTCTTATTTTGTGTATTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTCTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                   <  1:4516661/140‑1 (MQ=255)
                                                                                  aGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGc                                                  >  2:3535541/1‑140 (MQ=255)
                                                                                        aGCTGAGAAGCTGAGTCTTTTGTTGTGTTTTGTATGGTAAAGCGGCCGCTGTATTTTTTGTCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCg                                            >  2:2186527/1‑140 (MQ=255)
                                                                                                aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                    <  2:2241533/140‑1 (MQ=255)
                                                                                                aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                    <  1:194559/140‑1 (MQ=255)
                                                                                                  cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTTCTTTTTGTTTTTTTTTCTTTACCCGGTGAAAATTGTATTTTACCGTTTTCAGCCGGTCTTGTGTTTTTTGGGCAATACGGTGGTGGCAt                                  >  1:3633183/1‑140 (MQ=255)
                                                                                                  cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTTTTTGTTTTTTTTTCTTTCCCGGGTGAAAAGTGGATTTTCCCGTTTTAAGCCGTTCTTCTGTTTTTTTGGCAATAAGGTCGTGGCAt                                  >  1:2292476/1‑140 (MQ=255)
                                                                                                  cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGAGTTTTTCTTTTTTTTTCTTTCCCGGGTGAAAACTGAATTTTCCCGTTTTCAGCCGTTGTTCTGTTTTTTTGGGAATCCGGTCGTGGCAt                                  >  2:3265834/1‑140 (MQ=255)
                                                                                                  cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTATTTTTTTTGCTGTACCGGGTGAAAAGTTAATTTTCCCGTTTTAAGCCGGTCATATGTTTTTTGTGCAAACAGGTCGTTGCAt                                  >  2:2686773/1‑140 (MQ=255)
                                                                                                    gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGTCTTTTTGTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                >  2:1477791/1‑140 (MQ=255)
                                                                                                    gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTTTCCCGGGTGAAAAGTGAATTTTACCTTTTTCAGCCGTTCTTCTGTTTTTTTGGCAATCCGGTCGTGGCAttg                                >  2:4931245/1‑139 (MQ=255)
                                                                                                    gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTACCGGGTGACAAGTGAATTTTCCCGTTTTGAGCCGTTGTTCTGTTTTTTGGGCAATCCGGGCGTGGccata                                >  2:1966266/1‑136 (MQ=255)
                                                                                                    gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCGGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                <  2:2558085/140‑1 (MQ=255)
                                                                                                     aGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTGTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTcc                               <  2:3609428/140‑1 (MQ=255)
                                                                                                     aGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTcc                               <  2:1087519/140‑1 (MQ=255)
                                                                                                     aGTCTTTTTTTGTGTGTTGTAGGGTAAAGCGGCCGCTTTATTTTTGGACTTTTTCTATTTTTTGCTGTCCCGGGTGAAACGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTcc                               <  2:2813734/140‑1 (MQ=255)
                                                                                                       tCTTTTTTTGTTTTTTGTTTGGTAAACCGGCCGATTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                             <  2:1677750/140‑1 (MQ=255)
                                                                                                       tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                             <  1:2386747/140‑1 (MQ=255)
                                                                                                        cTTTTTTTTTGTTTTGTAGGGTACAGCGGCCGCGTTATTTTTTGACTTTTTCATTTATTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                            <  2:4625915/140‑1 (MQ=255)
                                                                                                        cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                            <  1:2686773/140‑1 (MQ=255)
                                                                                                        cTTTTTTTGTGTTGTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTGTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                            <  1:3265834/140‑1 (MQ=255)
                                                                                                          tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                           <  2:343048/140‑1 (MQ=255)
                                                                                                           ttttttttGTTTTTTATTGTAAAGCGGCCTCTTTATTTTTTGACTTTTTTTTTTTTTTGCTGTCCCGTTTGAAAAGTGAATTTTCACTTTTTCATCCGTTATTCTGTTTTTTGGGCAATCCGGTCGTTTCATTCCTGGca                          <  2:4004866/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAATGCGGCCGCTTTTTTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                          <  1:954866/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAACGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCGGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                          <  2:1742589/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                          <  2:1834628/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                          <  1:3995087/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                          <  1:2556928/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCCTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                          <  2:437638/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGATGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                          <  1:72182/140‑1 (MQ=255)
                                                                                                           ttttttCTGTTGTGTCTGGGAAACCCGCCGCTTTATTTTTTGACTTGTTGTTTTTTTTGCTGTCCCGGTTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATACCTGGca                          <  2:3577598/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                        <  2:4088120/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                        <  1:1091137/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                        <  2:1443649/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTGTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGGTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                        <  2:4664278/140‑1 (MQ=255)
                                                                                                              tttGTGTTTTGTATGGTAAAGAGGCCTCTTTATTTTTTTACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCATTTTTCAGCAGTTCTTCTGTTTTTTGGGCAATGCGGTCGTGGCATTCCTGGCACat                       <  1:636908/140‑1 (MQ=255)
                                                                                                              tttGTGTTTTGTATGGGACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                       <  1:2989507/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                      <  1:22383/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                      <  2:485539/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                      <  1:266487/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                      <  2:3404240/140‑1 (MQ=255)
                                                                                                               ttGGGTTTTGTAGGGTAAAGCGGCCGCGTTATTTTTTGACTGTTTCTCGTTTTGGCTGTCCCGGGTGAACAGTGAATTTGCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTCGCATTCCTGGCACata                      <  1:426449/140‑1 (MQ=255)
                                                                                                               gtgtgTTTTGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                      <  2:4884472/139‑1 (MQ=255)
                                                                                                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                     <  2:1539661/140‑1 (MQ=255)
                                                                                                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                     <  2:123007/140‑1 (MQ=255)
                                                                                                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                     <  2:1209980/140‑1 (MQ=255)
                                                                                                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTGCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                     <  2:266560/140‑1 (MQ=255)
                                                                                                                    ttttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                 <  1:1769581/140‑1 (MQ=255)
                                                                                                                    ttttGTATGGTAAAGCGGCCGCGTTATTTTTTTACTTTTTCTTTTTTTTTCTTTCACCGGTGAAAAATGAAATTTAACCTTTTAAGCCCTTATTATTTTTTTTTGTGAAAACGGTAGTTGCATTACATGCACaaataatt                 >  1:4652102/1‑137 (MQ=255)
                                                                                                                    ttttGTATGGTAAAGCGGCCGCGTTATTTTTTGACTTTTTCTTTTTTTTTATGTCCCGGGTGAAAAGTGAAATTTACCGATTTAAGCCGTTCTTATTTTTTTTTGGCAAACCGGTCGTTGCATTCCTGGCACataaaatt                 >  1:2149071/1‑135 (MQ=255)
                                                                                                                     tttGTATGGTAACGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgta                <  2:4528678/140‑1 (MQ=255)
                                                                                                                              tAAAGCGGCCGCTTTATTTTTTGCCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg       <  2:1164254/140‑1 (MQ=255)
                                                                                                                              tAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGACAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg       <  2:34703/140‑1 (MQ=255)
                                                                                                                              tAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg       <  2:3843706/140‑1 (MQ=255)
                                                                                                                              tAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg       <  2:229526/140‑1 (MQ=255)
                                                                                                                              tAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGGCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg       <  2:4571381/140‑1 (MQ=255)
                                                                                                                              tAAAGCGGCCGCTATATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAAGTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg       <  2:3326870/140‑1 (MQ=255)
                                                                                                                               aaaGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGc      <  1:4958822/140‑1 (MQ=255)
                                                                                                                               aaaGCGGCCGCTTTATTTTTTGACTTTGTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGc      <  1:667439/140‑1 (MQ=255)
                                                                                                                                 aGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTACCGTTTTAAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCgg    >  2:132894/1‑140 (MQ=255)
                                                                                                                                 aGCGGCCGCTTTAGTTTTTTTCTTTTTGTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGGTGTTCTTTTTTTTGGGCAATCCGGTCGTGGCATTACTGGCCCAAATATGTATGGAGTTGGCgg    >  2:3787242/1‑140 (MQ=255)
                                                                                                                                 aGCGGCCGCGTTATTTTTTTACTTTTTGTTTTTTTGGTGTCCCGGGTGAAAAGTGAATTTTCACGTTTTCAGCCGTTGTTCTGTTTTTTTGGCAAACCGGGAGTGGCAATCCTGGCACAAATAAGTATTGATTGGGGGGt   >  1:1529150/1‑140 (MQ=255)
                                                                                                                                   cGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTTTCCCGGGTGAAAAGTGAATTTTACCGTTTTCAGCCGTTCTTCTGTTTTTTTGGCAATCCGGTCGTGGCATTCCGGGCACCTATATGTATGGATTTGGCGGtt  >  2:2377079/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                    
TGATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTT  >  NZ_CP034943/1514717‑1514986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: