Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,950,238 G→A 6.5% I2995I (ATC→ATT EO946_RS09740 ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,950,2380GA 6.5% 1279.5 / 48.2 380I2995I (ATC→ATTEO946_RS09740non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (197/158);  new base A (12/13);  total (209/171)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 385 total aligned reads displayed.

AACGATCTTAGCCTCTGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCTTCATCATCAGGCCGCTGTTCTGCTT  >  NZ_CP034943/1950100‑1950377
                                                                                                                                          |                                                                                                                                           
aaCGATCTTAGCCTCTGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAgatga                                                                                                                                            >  1:1571617/1‑140 (MQ=255)
    aTCTTAGCCTCTGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGc                                                                                                                                        >  2:913297/1‑140 (MQ=255)
    aTCTTAGCCTCTGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGc                                                                                                                                        >  2:277343/1‑140 (MQ=255)
      cTTAGCCTCTGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGAGCCGCCGGCTTTTATAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGcaa                                                                                                                                      >  1:2502266/1‑140 (MQ=255)
               tGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGAt                                                                                                                             >  1:1704441/1‑140 (MQ=255)
               tGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGAt                                                                                                                             >  1:2735206/1‑140 (MQ=255)
               tGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGAGATGCGGGCAGATGATGGCAACAAAATGAt                                                                                                                             >  1:4980606/1‑140 (MQ=255)
               tGAATCCTTCAGCATATATTGTATCCCGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCTCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGAGGGCAGATGATGGCAACAAACTGAt                                                                                                                             >  1:2256639/1‑140 (MQ=255)
                         aGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAAc                                                                                                                   <  1:3971928/140‑1 (MQ=255)
                            atatatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGcc                                                                                                                <  1:537431/140‑1 (MQ=255)
                               tcttGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCTATGCGGGCAGATGATGGCAACTAACTGATCCGCTTGAACGCCCtt                                                                                                             <  2:289647/138‑1 (MQ=255)
                               tatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  1:1490051/140‑1 (MQ=255)
                               tatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  2:4098398/140‑1 (MQ=255)
                               tatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  2:4337692/140‑1 (MQ=255)
                               tatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  1:4868186/140‑1 (MQ=255)
                               tatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  1:1015465/140‑1 (MQ=255)
                               tatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  1:3232364/140‑1 (MQ=255)
                               tatTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTATTAGAACAGCCAAAATTCCAACACTCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  2:378034/140‑1 (MQ=255)
                                atTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  1:2025951/139‑1 (MQ=255)
                                    tATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTc                                                                                                        >  2:1215960/1‑140 (MQ=255)
                                    tATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTc                                                                                                        >  2:582605/1‑140 (MQ=255)
                                     aaccGGTCATCTGGATACTCCTGATCAATTGGCACGTAACAGCCGACGGCTTTTAGAACAGCCAAAAATCCAACAAGCAGCTCGATGAGATGCTGGCAGATGATCCCAACAAACTGAACCGCTTGAACGCACGTCGTTCt                                                                                                       >  2:1128868/3‑140 (MQ=255)
                                     aTCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCt                                                                                                       >  1:3785342/1‑140 (MQ=255)
                                     aTCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCt                                                                                                       >  2:229543/1‑140 (MQ=255)
                                     aTCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTGCGGTCt                                                                                                       >  2:3217809/1‑140 (MQ=255)
                                     aTCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCt                                                                                                       >  2:2331822/1‑140 (MQ=255)
                                     aTCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCt                                                                                                       >  2:2169783/1‑140 (MQ=255)
                                     aTCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCt                                                                                                       >  2:1838670/1‑140 (MQ=255)
                                      tCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCtt                                                                                                      >  2:3122343/1‑140 (MQ=255)
                                          gTCCTCTGGATACTCCGGCTCAATTGGCACGTCGGCGCCGCCGGCTGGTAGACCAGCCCCCTTGCCAACAAGCAGCTCGATGCGATGCGGGCAGATGCTGGCGCGCTACGGATCCGCTTGAGCGCCCTGCGTGCTTCACg                                                                                                  >  2:3895907/1‑140 (MQ=255)
                                           tCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGt                                                                                                 >  1:1061786/1‑140 (MQ=255)
                                           tCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGt                                                                                                 >  2:509267/1‑140 (MQ=255)
                                           tCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGt                                                                                                 >  2:4117814/1‑140 (MQ=255)
                                           tCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGt                                                                                                 >  2:3515010/1‑140 (MQ=255)
                                           tCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGt                                                                                                 >  2:346021/1‑140 (MQ=255)
                                           tCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGt                                                                                                 >  2:2255965/1‑140 (MQ=255)
                                           tCCTCTGGATACTCCGGACCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGt                                                                                                 >  2:4226676/1‑140 (MQ=255)
                                              tctGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACACTCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTgcg                                                                                              <  2:4364705/140‑1 (MQ=255)
                                              tctGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTgcg                                                                                              >  2:3265005/1‑140 (MQ=255)
                                              tctGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTgcg                                                                                              >  2:4820415/1‑140 (MQ=255)
                                              tctGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAAGTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTgcg                                                                                              >  2:3970662/1‑140 (MQ=255)
                                                     aCTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAAt                                                                                       >  1:2201719/1‑140 (MQ=255)
                                                       tCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             >  1:23176/1‑116 (MQ=255)
                                                       tCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTg                                                                                     >  1:4538924/1‑140 (MQ=255)
                                                       tCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTg                                                                                     >  1:1831355/1‑140 (MQ=255)
                                                       tCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTg                                                                                     >  1:4168669/1‑140 (MQ=255)
                                                         cGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGAt                                                                                   >  1:284035/1‑140 (MQ=255)
                                                            aTCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCtt                                                                                                             <  2:2011240/111‑1 (MQ=255)
                                                            aTCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTa                                                                                >  1:2819475/1‑140 (MQ=255)
                                                            aTCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTa                                                                                >  1:772328/1‑140 (MQ=255)
                                                            aTCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTa                                                                                >  1:4256793/1‑140 (MQ=255)
                                                            aTCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTa                                                                                >  1:1586162/1‑140 (MQ=255)
                                                            aTCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTa                                                                                >  2:157563/1‑140 (MQ=255)
                                                               aaTTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTAccc                                                                             <  1:1860178/140‑1 (MQ=255)
                                                               aaTTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTAccc                                                                             <  1:1405992/140‑1 (MQ=255)
                                                                 ttGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGc                                                                           >  2:1462616/1‑140 (MQ=255)
                                                                 ttGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGc                                                                           >  2:75708/1‑140 (MQ=255)
                                                                   ggCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTACCGTGCGCGCCAATTGATTTACCCGcgc                                                                         >  2:4903665/1‑138 (MQ=255)
                                                                   ggCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTc                                                                         <  2:4170762/140‑1 (MQ=255)
                                                                          aTGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGc                                                                  <  2:2130932/140‑1 (MQ=255)
                                                                            gcgcCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAAGTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTg                                                                <  2:3625082/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  1:346021/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  1:2918816/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  2:4868170/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  1:3119695/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  1:3515010/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  2:301962/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  1:708137/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  1:3899751/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  2:2588578/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  2:1061786/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  2:1900896/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  1:4820415/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  2:1504529/140‑1 (MQ=255)
                                                                              gccgccGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGcc                                                              <  2:365716/140‑1 (MQ=255)
                                                                               ccgccgGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGccc                                                             <  1:1970298/140‑1 (MQ=255)
                                                                                  ccgGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTa                                                          >  1:596636/1‑140 (MQ=255)
                                                                                        tttAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCa                                                    <  1:2669898/140‑1 (MQ=255)
                                                                                        tttAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCa                                                    >  2:4546188/1‑140 (MQ=255)
                                                                                        tttAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCa                                                    <  1:4115690/140‑1 (MQ=255)
                                                                                               cAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACACACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGGACGTGCACCGTTCa                                             <  1:3288838/140‑1 (MQ=255)
                                                                                                 gCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCAtt                                           >  2:2943547/1‑140 (MQ=255)
                                                                                                 gCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCAGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCAtt                                           >  2:1351279/1‑140 (MQ=255)
                                                                                                  ccAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATAATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCAttt                                                                     <  1:971343/113‑1 (MQ=255)
                                                                                                    aaaaTTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGcc                                        <  2:3145002/140‑1 (MQ=255)
                                                                                                    aaaaTTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGcc                                        <  1:3681854/140‑1 (MQ=255)
                                                                                                    aaaaTTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGcc                                        <  1:1279506/140‑1 (MQ=255)
                                                                                                    aaaaTTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGcc                                        <  2:284035/140‑1 (MQ=255)
                                                                                                    aaaaTTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGcc                                        <  2:4644711/140‑1 (MQ=255)
                                                                                                     aaaTTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCa                                       <  1:3153686/140‑1 (MQ=255)
                                                                                                            aacaaTCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGccc                                                             <  1:12007/111‑1 (MQ=255)
                                                                                                            aacaaTCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATaa                                >  2:670145/1‑140 (MQ=255)
                                                                                                            aacaaTCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATaa                                <  1:2372019/140‑1 (MQ=255)
                                                                                                             acaaTCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAAc                               >  2:2460933/1‑140 (MQ=255)
                                                                                                                 tCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCt                           >  1:90166/1‑140 (MQ=255)
                                                                                                                          tgcgatgcgGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCTtcatcatca                  <  2:4055687/140‑1 (MQ=255)
                                                                                                                          tgcgatgcgGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCGtcatcatca                  <  2:1594822/140‑1 (MQ=255)
                                                                                                                             gatgcgGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCTTCATCATCAGGc               <  1:4398921/140‑1 (MQ=255)
                                                                                                                               tgcgGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCTTCATCATCAGGCCg             >  1:814075/1‑140 (MQ=255)
                                                                                                                                    gCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCTTCATCATCAGGCCGCTGtt        >  1:660394/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AACGATCTTAGCCTCTGAATCCTTCAGCATATATTGTATCCGGTCCTCTGGATACTCCGGATCAATTGGCACGTATGCGCCGCCGGCTTTTAGAACAGCCAAAATTCCAACAATCAGCTCGATGCGATGCGGGCAGATGATGGCAACAAACTGATCCGCTTGAACGCCCTTCGTTCTTAACGTGCGCGCCAATTGATTTACCCGCTCATTTAGCTGCCCGTACGTGCACCGTTCATTGCCAAATATAACGGCTTCATCATCAGGCCGCTGTTCTGCTT  >  NZ_CP034943/1950100‑1950377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: