BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,528	G→T	5.6%	Q57K (CAA→AAA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,528	0	G	T	5.6%	806.9 / 7.7	306	Q57K (CAA→AAA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base G (44/232); new base T (25/3); total (69/237)
Fisher's exact test for biased strand distribution p-value = 3.00e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

CGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGA  >  NZ_CP034943/2214390‑2214667

  ccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGaaa                                                                                                                                          <  1:1596183/140‑1 (MQ=255)

  ccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGaaa                                                                                                                                          <  1:64156/140‑1 (MQ=255)

       aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAAAAATg                                                                                                                                     >  2:330515/1‑140 (MQ=255)

       aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGTaataatt                                                                                                                                     >  1:3055658/1‑139 (MQ=255)

         gttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGtt                                                                                                                                   <  1:4133262/139‑1 (MQ=255)

         cTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGtt                                                                                                                                   <  1:4459188/140‑1 (MQ=255)

               taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTc                                                                                                                             <  2:2779089/140‑1 (MQ=255)

                aatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                            <  2:3263123/140‑1 (MQ=255)

                aatCATTTTGCTCAGTTTGTGGATGTTTTTCGCTGTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                            <  2:4783053/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATCGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTTGTCAct                                                                                                                          >  2:4087595/1‑140 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:4201570/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:2172148/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:3396363/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:4375868/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:735813/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:855274/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:3081839/140‑1 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTATATATTTTTTAAATAATTTTTTGGtaaat                                                                                                                          >  2:2656729/1‑136 (MQ=255)

                  tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTAAGATACAAACCGATTGATTTAATATGCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCTGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:2678876/140‑1 (MQ=255)

                  tCATTTTGCTCAGTGTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGACCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:2649123/140‑1 (MQ=255)

                    aTTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCActct                                                                                                                        >  2:1917413/1‑140 (MQ=255)

                     ttttGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTg                                                                                                                       <  2:900922/140‑1 (MQ=255)

                          cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                                  <  2:206779/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACACACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:2224846/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACCGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:2009023/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:2994168/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:2552201/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:658468/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:3369488/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:1985848/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:4196337/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:2126925/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:3339114/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:3802395/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:2630450/140‑1 (MQ=255)

                             aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAAGATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:4854736/140‑1 (MQ=255)

                               tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:3179028/140‑1 (MQ=255)

                               tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:463172/140‑1 (MQ=255)

                               tttttGGATGTTTTTAGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:2344954/140‑1 (MQ=255)

                               tttttGGATGTTGTTCGCTTGATTACGAGACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:3490882/140‑1 (MQ=255)

                               tttgtGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATCTCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:3288587/140‑1 (MQ=255)

                                         tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                                  <  1:2153312/125‑1 (MQ=255)

                                         tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                                  <  1:1386324/125‑1 (MQ=255)

                                         tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATGTTTTGGTGACTCTGTTTACAAGTGCATCTGTaaa                                                                                                   >  1:180007/1‑138 (MQ=255)

                                         tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCATGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGACTTCCGGCCGTTTATATTTTTGAAATAATTTTTTGGTCACTCTTTTTACAAGTGCATCAGTACa                                                                                                   >  2:2351137/1‑140 (MQ=255)

                                         tttttCGCTTTATTACGATACAAACCGATTGAGTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTGCGGCGGTTTATATTTTTTTAATAATTTTTTGGTGAGGCTGTTTACAATTGCATCTGTaaa                                                                                                   >  1:178353/1‑138 (MQ=255)

                                              cGCTTTATTACGATACCAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTACATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  1:4835380/140‑1 (MQ=255)

                                              cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  1:612112/140‑1 (MQ=255)

                                              cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  2:1377540/140‑1 (MQ=255)

                                              cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  1:2490559/140‑1 (MQ=255)

                                              cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  1:4774897/140‑1 (MQ=255)

                                              cGCTTTATGACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  1:2458600/140‑1 (MQ=255)

                                              cGCTTTATGACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTGTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  2:3117337/140‑1 (MQ=255)

                                                  ttattaCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTCCCCGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGa                                                                                          <  1:4502841/140‑1 (MQ=255)

                                                   tatttCGATACAACCCGATTGATTTAATATCCTGCTGTAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATTTTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATAGGGCACAt                                                                                         >  2:4533973/1‑140 (MQ=255)

                                                             cAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATTTTTTTGTCACACTGTTTTAAAGTGCATCAGTACAATTCGCCGATAAAGCGAcag                                                                               >  1:1965032/1‑138 (MQ=255)

                                                                aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:241192/94‑1 (MQ=255)

                                                                    ctggacttAATATCCTGCTGTAGCTCTTCCAGCGAAAGAGCCAACTCGTCTTCCGGCCGTGGATATCTTAGAAATAATGTTTTGGTCCCTCTGTTGACACGGGCATCGGTACATTGCGCCGATAAAGCGACTGCTACCAc                                                                        <  2:1598223/134‑1 (MQ=255)

                                                                    attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAATTTTTTTGTGACTCTGTTTAAAAATGAATGTGTACATTTCGCAGATAAAACGACTGCTAacac                                                                        >  1:2196314/1‑140 (MQ=255)

                                                                     ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:4859198/102‑1 (MQ=255)

                                                                     ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTAACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACt                                                                       >  2:4052271/1‑140 (MQ=255)

                                                                            aaTATCCTGCTGAAGCTCTTCCAGCGACACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                                <  1:4048969/140‑1 (MQ=255)

                                                                            aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                                <  2:1304517/140‑1 (MQ=255)

                                                                            aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGTAAAAAAGTTTTTGTGACACTGTTTAAAAGTGCAACGGTAAAATGCGCCGATAAATCGACTGCTACCACAAAtaaaa                                                                >  1:1417907/1‑136 (MQ=255)

                                                                                 ccTGCTCAAGCTCTTCCAGCGAAACAGACAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATTTTTTGGTCACTCTGTTTACAATTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGa                                                           <  1:1452664/140‑1 (MQ=255)

                                                                                  gtgctgAAGCTCTTCCAGCGAAACAGCCAAATAGGCTTCCGGCGGTTTGTGTTTTTGTTATAATGGTTTTGTCACTCTGTTTTCCAGTGCCTCAGTACCTTGCGCAGATAAAGCGAGAGCGACCACGAATTCAAAAGGAt                                                          >  2:4704903/2‑140 (MQ=255)

                                                                                  ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTGTATTTTTTTAATAATGTTTTTGTGACTCTGTTTTAAAGTGGATCTGTACATTGCGCAGATAAAACGACTGCTACCACTAATTAAAAAGGAt                                                          >  1:579824/1‑140 (MQ=255)

                                                                                  ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTATATTTTTGAAATAATGTTTTTGTCACTCTGTTTACAAGTTCAACTGTACATTTCGCAGATAAAGCGACTGCTACCACTAATTGAAAAGGAt                                                          >  2:305048/1‑140 (MQ=255)

                                                                                   tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAAAAAATTTTTGGTGACGCAGTTTTAAAATGAATCTGTAAAATTCGCAGATAAAACGGCAGCTAACAAAAAATCAAAAAGAtt                                                         >  1:166950/1‑140 (MQ=255)

                                                                                   tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGGTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAtt                                                         >  2:1146778/1‑140 (MQ=255)

                                                                                   tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATATTTGAAATAAGGTTTTGGTCACGGTGTTTACACGGGCATCAGTAGATTGGGCAGATAAATCGACTGCTACCACTAATACAAAAGGAtt                                                         >  2:2702145/1‑140 (MQ=255)

                                                                                   tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTAGATTTTTGAAAAAAAGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTAACCCGAATTAAAAAGGttt                                                         >  2:737107/1‑140 (MQ=255)

                                                                                       gAAGCTCTTCCCGCGAAACAGCCAAAGAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTGGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                     <  1:4087595/140‑1 (MQ=255)

                                                                                       agacagctTCCAGCGAAACAGCCAAATAGTCGTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                     <  2:4600837/134‑1 (MQ=255)

                                                                                        aaGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                          >  2:2944976/1‑134 (MQ=255)

                                                                                         agatctTCCAGCGAAACAGCCAAATAGACTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATg                                                   <  2:4920406/137‑1 (MQ=255)

                                                                                         aGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATg                                                   <  1:3172816/140‑1 (MQ=255)

                                                                                         aGCTCTCCCAGCGAAACAGCAAAATAGTCGTCCGGCCTTTTATATTTTTGAAATAATGTTTTGCTCACTCTGTTTACAAGTGCATCTGTCAATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATg                                                   <  1:3928920/140‑1 (MQ=255)

                                                                                           ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:78812/140‑1 (MQ=255)

                                                                                            tctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAc                                                <  2:3943797/140‑1 (MQ=255)

                                                                                             ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  2:579824/140‑1 (MQ=255)

                                                                                             ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  1:986322/140‑1 (MQ=255)

                                                                                             ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  2:4351220/140‑1 (MQ=255)

                                                                                             ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  1:11213/140‑1 (MQ=255)

                                                                                               tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATGTTTTTGTGACTCTGGTTAAAACTGCAACTGTACCTTGCGCAGATAAAGCGACTGCTAACACAAATTCAAAAGTATTGGAATGAAACAg                                             >  1:4939147/1‑140 (MQ=255)

                                                                                                 cAGCTAAACATCAAAATTGTCTTGCGTCCTTTTATATTTTTGAAATATTTTTTTGGTCACTCTGTTTAAAAGTGCTTCTGTACATTGCGCAGTTAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  1:2903794/140‑1 (MQ=255)

                                                                                                 cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  1:1001908/140‑1 (MQ=255)

                                                                                                     gAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTcaca                                       <  2:2003168/140‑1 (MQ=255)

                                                                                                      gaaCAGCCAAATAGTCTTCCGGCCGTGTATTTTTTTGGAATAATGTTGTGGTCACGCTGGTTTCAAGTGCATCAGTACATTGCGCCGATAAAGCAACAGCCCCCACTAATTCAAAAGGATTGGAAAGAACCAGGTCacac                                      >  2:226915/2‑140 (MQ=255)

                                                                                                          aGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                                  <  1:3575399/140‑1 (MQ=255)

                                                                                                           gcaaaaTAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGc                                 <  1:1255832/137‑1 (MQ=255)

                                                                                                             cacatcgTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCgt                               <  2:1538312/134‑1 (MQ=255)

                                                                                                             cAAATAGTCTGCCGGCCGTGTATCTTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGAGAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCgt                               <  2:283025/140‑1 (MQ=255)

                                                                                                                    tCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTAAAATGCGCAGATAAAGCGACTGCTAACACTAATTAAAAATGAATGGAATGAACCAGTTCAAACTCTGAGTTTGGaaa                        >  2:1101933/1‑140 (MQ=255)

                                                                                                                            ccGTTTATATTTATGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                <  2:3665267/140‑1 (MQ=255)

                                                                                                                              gTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTTCAAGTGCATATTTACATTGCGCAGATAAATCGACTGCTAACACTAATTCAAAAAGATTGTAATGAACCAGTTCAAACTCTGCGTGTGGAAACAAGTCGCCt              >  2:1237272/1‑140 (MQ=255)

                                                                                                                                ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  1:1374857/140‑1 (MQ=255)

                                                                                                                                ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  2:3480824/140‑1 (MQ=255)

                                                                                                                                ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  2:166950/140‑1 (MQ=255)

                                                                                                                                          gAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGa  <  2:3510855/140‑1 (MQ=255)

CGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGA  >  NZ_CP034943/2214390‑2214667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`