Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,379,799 T→G 6.3% K382T (AAG→ACG)  EO946_RS12160 ← sigma‑54‑dependent Fis family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,379,7990TG 6.3% 578.0 / 3.1 231K382T (AAG→ACG) EO946_RS12160sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (110/92);  new base G (21/3);  total (136/95)
Fisher's exact test for biased strand distribution p-value = 1.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

AGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGACACATC  >  NZ_CP034943/2379664‑2379935
                                                                                                                                       |                                                                                                                                        
aGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTGGGTATTGATTGCATGGGCGAACAGTTGTTTTAATGTTACCGATTACCCGCGCCACAAAAAAGTTTCCGGTGTTTTAGCCCCGCGCGTCGc                                                                                                                                      >  1:1842612/1‑140 (MQ=255)
aGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGGTGGGATGGGCGAACCGTTGTTTTCCTGTTGGCGATTCCCCGGGGCACCAAATCGTTGGCGGTGGTTTCGCCCCGCGCGTCgg                                                                                                                                      >  1:4297921/1‑139 (MQ=255)
aGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGc                                                                                                                                      <  1:3183513/140‑1 (MQ=255)
aGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGc                                                                                                                                      <  1:1861407/140‑1 (MQ=255)
aGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGAGTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGc                                                                                                                                      <  1:1040134/140‑1 (MQ=255)
aGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCGTTCGTTATGGATGGGATGGGCGCACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGGTTTCGCCCCGAGCGTCGc                                                                                                                                      >  1:29741/1‑140 (MQ=255)
aGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCATTCGTTATGGATGGCAGGGGCGTACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGc                                                                                                                                      <  1:4983207/140‑1 (MQ=255)
 gtgcagCACAGTTCACGCGAATAAATTTATTTAATTTAATTTATATTTCGTTATTGATTGCATGGGCGAACAGTTATTTTAAAGTTACCGATTACAAGCGCAACAAAATCGTTGCCCGTGTTTTAGACCCGAGCTTAGcc                                                                                                                                     >  2:3227026/6‑140 (MQ=255)
 gggCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGcc                                                                                                                                     >  2:3126796/1‑140 (MQ=255)
  ggCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCGTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCCGTGTTTTCGCCCCGAGCTTCGccg                                                                                                                                    >  2:3107970/1‑139 (MQ=255)
     tGCACAGTTCACTCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGtt                                                                                                                                 <  1:132283/140‑1 (MQ=255)
     tGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGtt                                                                                                                                 <  1:519344/140‑1 (MQ=255)
       cacaGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTGGGTATTGATGGCAAGGGCGAACAGTTATTTTCCTGTTCCCGATTACCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCGTGGCCTGTTCa                                                                                                                               >  2:348966/1‑140 (MQ=255)
       cacaGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTGGGTATGGATGGGATGGGCGAAAAGTTGTTTTACTGTTGCCCATTCCCCGCGCAACAAAAACGTTTCCGGGGTTTTCGCCCCGAGCGTCGCCTGTTcc                                                                                                                               >  1:1738781/1‑139 (MQ=255)
       cacaGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACCGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTTCCGGTGTTTTAGCCCCGAGCTTCGCCTGTTCa                                                                                                                               >  2:4078498/1‑140 (MQ=255)
       cacaGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTGTTTTCCTGTTCCCGATTCCCCGCGCACCAAAATCGTTGCCGGTGTTTTCGCCCCGAGCGTCGGCTGTTCa                                                                                                                               >  2:3748269/1‑140 (MQ=255)
       cacaGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCa                                                                                                                               >  2:3860706/1‑140 (MQ=255)
       cacaGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCa                                                                                                                               >  2:4590943/1‑140 (MQ=255)
           gTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAgcg                                                                                                                           <  1:3424808/140‑1 (MQ=255)
           gTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAgcg                                                                                                                           <  1:4716388/140‑1 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTGGTTATGGATTGCATTGGCGAACAGTTGTTTTCCTGTTGCCGATTACCCCCGCAACAAAAAAGTTGCCGGTGTTTTAGCCCCGAGCGTCGCCTGTTAAAgcgc                                                                                                                          >  1:4328926/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGTTTGCCTGGGCGCACAGTTCTTTTCCTGTTCCCCATTCCCCGCGCAACAAAATACTTGCCGGGGTTTTCGCCCCGAGCTTCGCCTCTTCAAgcgc                                                                                                                          >  2:2560559/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGTTGGCATGGGCGAACAGTTCTTTTGCTGTTGCCGATTACCCGCGCAACAAAATCGTTGCCGGGGTTTTGGCCCCGAGCTTGGCCGGTTCAAacgc                                                                                                                          >  1:4040590/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGCTGCCATGGGGGAACAGTTCTATTCGTGTTCCCGAATCCCCCCGCAACAAAAAAGTTGCCGGGGTTTTGGCCCCCAGCTTCGCCTTTTGaatccc                                                                                                                          >  2:2833765/1‑136 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATTGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTTCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAgcgc                                                                                                                          >  1:1160106/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCCTGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACCAAATCGTTGCCGGGGTTTTCGCCCCGAGCTTCGCCTGTTCAAgcgc                                                                                                                          >  2:4546397/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATTGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCCCGCAACAAAAAAGTTTGCGGTGTTTTCGGCCCGAGCGTCGCCTGTTGAAgcgc                                                                                                                          >  1:3365409/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTAGCCCCGCGCTTCGCCTGTTCAAtcgc                                                                                                                          >  2:4188294/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCATTTCCTGTTCCCGATTCCCCGCGCAACAAAAACGTTGGCGGTGTTTTCGCCCCGAGCTTAGCCTGGTCCAgcgc                                                                                                                          >  2:3051499/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCAAGGGCGCACAGTTCTTTTGCGGTTGCCCATTCCCCGCGCCCCAAAATCGTTGCCGGGGTTTTCGCCCCGAGCTTCGCCTGTTCAAgcgc                                                                                                                          >  2:4022842/1‑140 (MQ=255)
            ttCACGCGAATAAATTTATTGTATTTCCGGTCGCGTTCGTTATGGCTGGCATGGGCGAACCGTTGTTTTCCTGGTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGGGTTTTCGCCCCGAGCGTCGCCTGTTCTAgcgc                                                                                                                          >  1:2966617/1‑140 (MQ=255)
            ttCACGCGAATAAATTGATTGTATTTCCGGTCGCTTTCGGTATGGATGGCATGGGCGCACCGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGGTTTCGCCCCGAGCTTCGCCTGTTCAAgcgc                                                                                                                          >  2:582867/1‑140 (MQ=255)
             tCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCa                                                                                                                         <  2:4269488/140‑1 (MQ=255)
               aCGAGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAAc                                                                                                                       <  2:689941/140‑1 (MQ=255)
                cgcgAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAAcc                                                                                                                      <  1:2579164/140‑1 (MQ=255)
                 gcgAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTATTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCCCCTGTTCAAGCGCAACCa                                                                                                                     >  2:716519/1‑140 (MQ=255)
                 gcgAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGGTCTTTTACTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCa                                                                                                                     >  2:4746660/1‑140 (MQ=255)
                 gcgAATAAATTTATTGTATTTCCGGTCGCGTTCGTTATTGATGGCATGGGCGAACAGTTGTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCCGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCa                                                                                                                     >  1:316578/1‑140 (MQ=255)
                 gcgAATAAATTTATTGTATTTCCGGTCGCGTTCGTTATTGATGGCATGGGCGAAAACTTGTTTTACTGTTCCCCATTACACGCGCAACAAAAAAGTTTCCCGTGTTTTGGCCCCGAGCGTTTCCCGTTCAAACGCAACCa                                                                                                                     >  1:4525991/1‑140 (MQ=255)
                  cgAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAg                                                                                                                    <  1:1426232/140‑1 (MQ=255)
                    aaTAAATTTATTGTATTTCCGGTCGCTTTGGTTATTGATGGCATGGGCGAACAGTTCTTTTGCTGTTGCCGATTCCCCCCGCACCAAAAACGTTTCCGGGGTTTTCGCCCCCAGCTTCGCCGGTTCAAGCGCAAccacca                                                                                                                  >  1:323416/1‑140 (MQ=255)
                      tAACTTTATTGTATTTCCGGTCGCTTTGGTTATGGATGGCATTGGCGAACACTTGTTTTCCAGTTCCCGCTTCACCGCGCCACAAAATATTTGCCGGTGTTTTAGGCCCGAGCTTCGCATGTTAACGCGCAACCACCAtt                                                                                                                >  1:551756/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTGGTTATTGATGGCATGGGCGAACACTTGTTTTGCAGGTGCCGAGTCCCCCCGGAACAAAATCGTTGCCGGTGTTTTGTCCCCGCGCTTCGCCTGTTCCAGCGCAACCCGCAtt                                                                                                                >  1:225004/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATTGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCAtt                                                                                                                >  2:1171841/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATTGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCCACCAGCAtt                                                                                                                >  1:1730301/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCCTGGGCGAACAGTTCTTTTACGGTTCCCCATTCCCCGCGCAACAAAATAGTTGCCGGTGTTTTCGCCCCGAGCTTCCCCGGTTCAAGCGCAAACCGCaat                                                                                                                >  2:3192640/1‑138 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATTGGCGAACAGGTATTTTACTGTTAACGATTACCCCCCCAACAAAAACGGTTCCGGTGTTTTGGCCCCCAGCTTGGCCGGTTAAAGCGCAAcccacagt                                                                                                                >  1:37306/1‑134 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCaat                                                                                                                >  2:4827773/1‑138 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCAtt                                                                                                                >  2:1846838/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCAtt                                                                                                                >  2:317827/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCAtt                                                                                                                >  2:2594750/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCAtt                                                                                                                >  2:254610/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCGGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGGGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCAtt                                                                                                                >  2:279443/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCGTTGGTTATTGATGGCATGGGCGAAAAGTTCTTTTCCTGGTGCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCCACCCTCGCCGGTTCAAGCGCAACCACCAtt                                                                                                                >  1:860087/1‑140 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCGTTGGGTATTGATTGCATTGGCGAACAGTTATTTTCCTGTTCCCGATTACCCCCGCAACAAAATACTTGCCCGTGTTTTGGCCCCCAGCGTCGCCGGTTAAAGCGCAACCaagatt                                                                                                                >  1:3543588/1‑135 (MQ=255)
                      tAAATTTATTGTATTTCCGGTCGCGTTCGTTATGGATTGCAGGGGCGAACAGTTGGTTTCGTGTTTACGGTTCCCCCCGCAACAAAAACGTTGCCGGTGTTTTAGCCCCGAGCTTAGCCTGTTCAAGCGCAACCACCAtt                                                                                                                >  1:1957656/1‑140 (MQ=255)
                                          tCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAAt                                                                                            <  1:3071608/140‑1 (MQ=255)
                                               ttCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATgtcgt                                                                                       <  1:4908541/140‑1 (MQ=255)
                                               ttCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATgtcgt                                                                                       <  1:3949888/140‑1 (MQ=255)
                                                 cGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCa                                                                                     <  1:4170535/140‑1 (MQ=255)
                                                    tATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAAc                                                                                  <  1:3227026/140‑1 (MQ=255)
                                                      tggatggCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCCTTTGCTCGCTTTTGCCGATAATGTCGTCAAACgt                                                                                >  2:135964/1‑140 (MQ=255)
                                                        gatggCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACgtgt                                                                              >  2:3458426/1‑140 (MQ=255)
                                                           ggCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTAtt                                                                           <  1:3669645/140‑1 (MQ=255)
                                                           ggCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTAtt                                                                           <  1:2481833/140‑1 (MQ=255)
                                                                gggCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCt                                                                      >  1:2442998/1‑140 (MQ=255)
                                                                 ggCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCtt                                                                     >  1:2422593/1‑140 (MQ=255)
                                                                  gCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTc                                                                    >  2:2339317/1‑140 (MQ=255)
                                                                  gCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTc                                                                    >  2:1287697/1‑140 (MQ=255)
                                                                  gCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTc                                                                    >  2:1106099/1‑140 (MQ=255)
                                                                     aaCAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAg                                                                 <  2:174557/140‑1 (MQ=255)
                                                                     aaCAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAg                                                                 <  2:160485/140‑1 (MQ=255)
                                                                     aaCAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAg                                                                 <  2:1116377/140‑1 (MQ=255)
                                                                       cAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCg                                                               >  1:3490966/1‑140 (MQ=255)
                                                                           tCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCg                                                           <  2:228100/140‑1 (MQ=255)
                                                                                  cTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCACCCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGAtt                                                    >  2:189102/1‑140 (MQ=255)
                                                                                  cTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGAtt                                                    >  2:3371497/1‑140 (MQ=255)
                                                                                    gTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCa                                                                                     <  2:4172223/105‑1 (MQ=255)
                                                                                    gTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCa                                                                                     >  1:4172224/1‑105 (MQ=255)
                                                                                    gTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTg                                                  >  1:2158051/1‑140 (MQ=255)
                                                                                    gTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATGTTGCTTCAAGCGTCCGAATGATTTg                                                  >  2:4090523/1‑140 (MQ=255)
                                                                                           agtCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTAGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGgcg                                           <  2:4119973/138‑1 (MQ=255)
                                                                                           aTTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGgcg                                           <  2:485339/140‑1 (MQ=255)
                                                                                           aTTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGgcg                                           <  2:1946116/140‑1 (MQ=255)
                                                                                               cccGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCt                                                                                                            >  2:44790/1‑71 (MQ=255)
                                                                                               cccGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGt                                       <  2:3008398/140‑1 (MQ=255)
                                                                                               cccGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGt                                       >  2:1498781/1‑140 (MQ=255)
                                                                                                 cgcgCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTa                                     >  2:17113/1‑140 (MQ=255)
                                                                                                  gcgcAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTaa                                    >  1:2693874/1‑140 (MQ=255)
                                                                                                      aacaaAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTc                                >  1:2762615/1‑140 (MQ=255)
                                                                                                                    cGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCAttt                  <  1:4188294/140‑1 (MQ=255)
                                                                                                                           ttCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTc           >  1:3499618/1‑140 (MQ=255)
                                                                                                                             cGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCgg         <  2:2717777/140‑1 (MQ=255)
                                                                                                                               ccccGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGac       <  2:2158051/140‑1 (MQ=255)
                                                                                                                                cccGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGaca      <  2:3704400/140‑1 (MQ=255)
                                                                                                                                cccGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGaca      <  1:3956167/140‑1 (MQ=255)
                                                                                                                                  cGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGAcaca    <  1:3860706/140‑1 (MQ=255)
                                                                                                                                  cGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGAcaca    <  1:4590943/140‑1 (MQ=255)
                                                                                                                                  cGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGAcaca    <  1:44358/140‑1 (MQ=255)
                                                                                                                                    aGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGACACATc  <  1:17113/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
AGGGCTGCACAGTTCACGCGAATAAATTTATTGTATTTCCGGTCGCTTTCGTTATGGATGGCATGGGCGAACAGTTCTTTTCCTGTTCCCGATTCCCCGCGCAACAAAATCGTTGCCGGTGTTTTCGCCCCGAGCTTCGCCTGTTCAAGCGCAACCAGCATTTGCTCGCTTTTGCCGATAATGTCGTCAAACGTGTATTTTGCTTCAAGCGTCCGAATGATTTGCCTGGCGCGGTTAAGCTCCGCTGTCAGCATTTTAATTTCGGACACATC  >  NZ_CP034943/2379664‑2379935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: