Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,212 T→G 6.4% N284H (AAC→CAC)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2120TG 6.4% 537.9 / 3.0 233N284H (AAC→CAC) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base T (121/86);  new base G (24/2);  total (145/88)
Fisher's exact test for biased strand distribution p-value = 4.50e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

ACATGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCA  >  NZ_CP034943/2502076‑2502340
                                                                                                                                        |                                                                                                                                
acaTGCTGAAGCAGGTTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttc                                                                                                                               >  1:1214933/1‑140 (MQ=255)
acaTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGttttg                                                                                                                               >  1:563750/1‑139 (MQ=255)
acaTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttc                                                                                                                               >  1:3890941/1‑140 (MQ=255)
 caTGCTGACGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                              >  2:2322506/1‑140 (MQ=255)
 caTGCTGAAGCAGGCTTACAACCCTGGAATAATAAATGCGGGTCGTACCGATGATCGCAATTGAGCCGATATGCTTCTGTTCAACGGACTACGAAGCCGTAATCATACTGCAGTATTCCATCTCTTCATAGTAGTtttcg                                                                                                                              >  2:875417/1‑139 (MQ=255)
 caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                              >  2:2386805/1‑140 (MQ=255)
 caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                              >  2:2251551/1‑140 (MQ=255)
 caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATGGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttcg                                                                                                                              >  1:2250467/1‑139 (MQ=255)
        aatcAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCACTTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTcccca                                                                                                                       >  1:2001678/4‑138 (MQ=255)
            aGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGAtttt                                                                                                                   >  1:4832681/1‑140 (MQ=255)
             ggCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTa                                                                                                                  >  1:4814942/1‑140 (MQ=255)
              gCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCCGTTTTat                                                                                                                 >  1:2542636/1‑139 (MQ=255)
              gCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGGTTCCCGAttttga                                                                                                                 >  1:1525526/1‑138 (MQ=255)
              gCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTaa                                                                                                                 >  1:4138507/1‑140 (MQ=255)
              gCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTaa                                                                                                                 >  1:1081246/1‑140 (MQ=255)
              gCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTaa                                                                                                                 >  1:3711357/1‑140 (MQ=255)
               cTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCCGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAAt                                                                                                                <  1:246450/140‑1 (MQ=255)
                  aCAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGGTTTCCATCTCTTCATAGACGTTTTCTTTCCCGATTTTAATCGa                                                                                                             >  2:3614570/1‑140 (MQ=255)
                        cTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCGTTCCCGATTTTAATCGAAATccc                                                                                                       >  2:1107384/1‑140 (MQ=255)
                          ggAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAGGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTACTCGAAATCCCCg                                                                                                     <  1:813559/140‑1 (MQ=255)
                                atTCATGCGGGTCGGACCGATGCTCGCAATTGAGACGATCTGCTTCTGATCAACGGCATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGGTTGCTTTACCGAGTTTCATCGAAATCCCCGggggtg                                                                                               >  1:1161184/1‑140 (MQ=255)
                                atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGtgtgtg                                                                                               <  2:667332/140‑1 (MQ=255)
                                atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGtgtgtg                                                                                               >  2:2469569/1‑140 (MQ=255)
                                atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGtgtgtg                                                                                               >  2:1730692/1‑140 (MQ=255)
                                 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGTGTGTgg                                                                                              >  1:1064254/1‑140 (MQ=255)
                                 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAATCGAAATCCCCGGGTGTgg                                                                                              >  1:1481073/1‑140 (MQ=255)
                                 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTgg                                                                                              >  2:1642098/1‑140 (MQ=255)
                                 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTgg                                                                                              >  2:1440452/1‑140 (MQ=255)
                                 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTgg                                                                                              >  1:3951609/1‑140 (MQ=255)
                                 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCACGATTTTAATAGAAATAACCGGGTGTgg                                                                                              >  1:2032708/1‑140 (MQ=255)
                                      gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAtt                                                                                         >  2:3132446/1‑140 (MQ=255)
                                           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGTACg                                                                                    >  2:4604418/1‑140 (MQ=255)
                                           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                    <  2:1588159/140‑1 (MQ=255)
                                           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                    <  2:1500141/140‑1 (MQ=255)
                                           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                    <  2:1406146/140‑1 (MQ=255)
                                                     gatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaaa                                                                          <  1:2456598/140‑1 (MQ=255)
                                                       tagCAATTGAGCCTATCTGCTTCTTATCAACGTAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCCTAGACGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTTTGTGGGTTTTGAACGAGCTTTAAAACa                                                                        <  1:3550882/138‑1 (MQ=255)
                                                         gCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTACCGATTTTAATCGAAATCCCCCGGTGGGGGGATTGAACGAGCGTTAAAAAATc                                                                      >  1:2937595/1‑140 (MQ=255)
                                                                aGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCCTGTGTGGGGATTGAACGAGCTTTAAAACATACTGTTCt                                                               >  2:4173086/1‑140 (MQ=255)
                                                                  ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                             <  1:3478105/140‑1 (MQ=255)
                                                                  ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCatt                                                             >  2:4753849/1‑140 (MQ=255)
                                                                    gATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttt                                                           <  1:2133207/140‑1 (MQ=255)
                                                                     aTCTGCTTCTGGTCTACGGAATAAGAAGCCGTAATCAGACTGCAATTTTCCATCTCTTCATAGTCGTGTTATTTTACGATTTTAATCGAAATTCCCGTTTGGGGGGATTGAAACAGCGTTAAAACATGCTGTTGTTtctc                                                          >  1:2892136/1‑140 (MQ=255)
                                                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCg                                                                                                     <  2:3516275/97‑1 (MQ=255)
                                                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGGGGGGCTTGAACGAGCTTTAAAACATCCTGTTCTTTTTc                                                          >  2:4715919/1‑140 (MQ=255)
                                                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTc                                                          >  2:3640304/1‑140 (MQ=255)
                                                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTc                                                          >  2:106826/1‑140 (MQ=255)
                                                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATGCTGTTCttttta                                                          >  2:3288254/1‑139 (MQ=255)
                                                                       cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                        <  2:3934485/140‑1 (MQ=255)
                                                                         gCTTCTGATCACCGGCATAAGAAGCCGTAATCAGACTGCAGTTTTCCCTCTCTTCATAGTCGTTTTCTTTCCCGTTTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                      <  2:2975039/140‑1 (MQ=255)
                                                                                aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCGTTCCCGATTTTAATCGAAAAACCAGGGTGGGGGGTGTGAACGAGCGTTAAAACAGGCGGTTGTTTGTCCAtttaagtcg                                               >  1:3198618/1‑133 (MQ=255)
                                                                                aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTATTTCCCGATTTTAATCGAAATAACCCTGTGGGGGGTGTGAAAGCTCGTTAAAAAATACAGGTCTTTTTAAATTAAAgata                                               >  1:1822022/1‑138 (MQ=255)
                                                                                  cAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTAAATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCTAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCGTTTTCTTTTTCAATTAATgagaga                                             <  1:1752298/140‑1 (MQ=255)
                                                                                    aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                           <  2:1235506/140‑1 (MQ=255)
                                                                                    aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                           >  2:802613/1‑140 (MQ=255)
                                                                                    aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATgagagtct                                           >  2:2186355/1‑137 (MQ=255)
                                                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATTTTAATAGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACAACCTGTTGTTTTTCAATTAATGAGAGaaac                                          >  2:737680/1‑138 (MQ=255)
                                                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCCATTTTAAATGAAAAACCCGGGGGTGGGGATTTAACCAGCTTTAAAAATTCCTGTTTTTTTTGAATTAATGATAGAAGc                                          >  1:3069434/1‑140 (MQ=255)
                                                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTGCCGATTTTAATCGAAAAACCCCTGGGTGGGGCTTGTACGAGCGTTAAAACATCCTGTTATTTTTAAATTAATGAGAGAAGc                                          >  1:1582555/1‑140 (MQ=255)
                                                                                          tAAGAAGCCTTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCGTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                     <  1:2674977/140‑1 (MQ=255)
                                                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                     <  2:4379002/140‑1 (MQ=255)
                                                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                     <  2:1796141/140‑1 (MQ=255)
                                                                                            agaagaCGAAATCAGACTGCAGTTTTCCATCTCTTAATCGTCGTTTTCTTTCCCGATTTTAATCTAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGaa                                   <  1:2823842/140‑1 (MQ=255)
                                                                                              aagCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCGGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAct                                 <  1:1637515/140‑1 (MQ=255)
                                                                                                  cGTACTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTACCGATTTTAATCGAAAACCCCGGGTGTGGGGATTTAACGAGGTTTAAAAAATACAGTTCTGTTTAAATTAAAGTGAGAAGCCACCGCACACTgg                             >  2:573641/1‑140 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCGTTAAAACATCCGGGTGTTTTTCAATTAATGAGAGAAGCGACCGAAcgcggg                             >  2:491042/1‑135 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGGGTGGGGGGATTGAACGAGCGTTAAAACATCCTGTTCTTTTTGAATTAATGTGAGAAGCGACCGACCGCTgg                             >  1:4058093/1‑140 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCCTGGGGGGGGATGGAACGAGCTTTAAAACAGCCGGTTGTTTTTCAATTAATGGGTGAATCGCCCGaccggggg                             >  1:2765639/1‑133 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAAACCCCCTGGGGGGGGATTGGAAGCGCGTTAAAACATCCTGTTGTTTTTGAAATAATTGGGGAAGCGACCGAAcgaggg                             >  1:4566488/1‑135 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                             >  2:3825388/1‑140 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGATAAGCGAACGAActaggg                             >  2:4890855/1‑136 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAACCCCGGGTGTGGGGATTGAACGAGCGTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAAGCGACCCAAcaatgg                             >  2:2094042/1‑135 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAAAACCCGGGTGGGGGGATTGAAAGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCCAACTCTgg                             >  1:4953332/1‑140 (MQ=255)
                                                                                                  cGTAATCAGACTGCAGGTTTCCATCTCTTCATAGTCGTTTTCTTTACCCATTTTAATAGAAAAACCCCTGTGTGTGGTATGCACGTGGGTTAAAACATCATGTAGTTTTTGCATTAATGTGATAACCGCCCGaaagacgg                             >  1:3847733/1‑134 (MQ=255)
                                                                                                    tAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATAGAAATCCCCCGGGGTGGGGCGTGAAAGAGCTTTAAAAAATACCGTTGTTTTTTTATTAATTAAATAAGCGACCGAAAACTGGTg                           >  1:69863/1‑140 (MQ=255)
                                                                                                    tAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAAACGCAATCCCCGGGTGTGGGGATTGTACGAGCTTTAAAACATACTGTTGTTTTTGAATTAATGTGAGAAGCGACCGAACTCTGGTg                           >  1:3315593/1‑140 (MQ=255)
                                                                                                     aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGTATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                          <  2:499635/140‑1 (MQ=255)
                                                                                                     aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                          <  2:4946193/140‑1 (MQ=255)
                                                                                                     aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                          <  1:3474112/140‑1 (MQ=255)
                                                                                                     aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                          <  2:3951609/140‑1 (MQ=255)
                                                                                                     aaTCAGAATGCATTTTTCCATCTCTTAATAGTCGTTTTCTTTCCCGATTTTAATCTAAATCCCCGTGTGTGGGTATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                          <  1:4019889/140‑1 (MQ=255)
                                                                                                       tCAGACTGCAGTTTTCCCTGTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                        <  2:3310537/140‑1 (MQ=255)
                                                                                                       tCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                        <  2:2437380/140‑1 (MQ=255)
                                                                                                       tCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                        <  2:2615035/140‑1 (MQ=255)
                                                                                                       tCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                        <  2:4814942/140‑1 (MQ=255)
                                                                                                       tCAGACTGCAGTGTGCCATCTCTTCATAGTCGTTTTCTGTCCCGATGTTAATCGAAATCCCCGTGTGTGGGGATTGCACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                        <  2:3870098/140‑1 (MQ=255)
                                                                                                           aCTGCATTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATTTTAATCGAAAACCGCGTGTTTTGGGATTGAAAGTGCTATAAAATAACGAGGTCTTTTTAAAATAAATATAGAACCGACCGAAAACAGGCGATATGAt                    >  2:2872436/1‑140 (MQ=255)
                                                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATAGAAAAACCCGGGTGTGGGGATTTAACGAGCTTTAAAAAAAACTGTTGTTTTTAAAATAAAGAGAGAACCGACCCAAATCTGGTGTTATGAt                    >  1:4424493/1‑140 (MQ=255)
                                                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCAt                    >  2:2797640/1‑140 (MQ=255)
                                                                                                            cTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATg                   <  2:2518540/140‑1 (MQ=255)
                                                                                                                 gTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACt              <  2:1993684/140‑1 (MQ=255)
                                                                                                                  ttttCCATCTCTTCATAGTCGTTTTGTTTACCGAATTTAATCGAAATAACCGTGTGTGGGGATTGAAAGAGCTTTAAAACATACTGTTGTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGTTGGTATAATGGAACTc             >  2:3246524/1‑140 (MQ=255)
                                                                                                                  tgttCCATCTCTTCTTAGTCGTTTTCTTTCCCGATTTTTATCGAAATCACCGTGTGTGGGGATTTTACGAGCTTTAAAACATCCTGTTCTTTTTCAAATAATGAGAGAAGCGACCGAACACTGGGGGTATCATGGAACTc             >  2:3489274/3‑140 (MQ=255)
                                                                                                                         tctcTTCATAGTCGTTTTGTTTCCCGATTTTAATAGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTAAATTAATTATAGAAGCGAACGAACTCTGGTTATATCATGGAAATACGGCTgg      >  2:2516206/1‑140 (MQ=255)
                                                                                                                         tctcTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTATTTTTAAATTAATTAGAGAAGCGACCGAAATCTGGTGATATAATTGAACTCCCGCTgg      >  2:342240/1‑140 (MQ=255)
                                                                                                                           tctTCATATTCGTTTTCTTGCCCGATTTTACTCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGACAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt    <  1:4173086/140‑1 (MQ=255)
                                                                                                                           tctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt    <  1:2339646/140‑1 (MQ=255)
                                                                                                                           tctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt    <  1:3960127/140‑1 (MQ=255)
                                                                                                                           tctTCATAGTCGTGTTCTTGCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt    <  1:4671910/140‑1 (MQ=255)
                                                                                                                             ttCATAGTCGTTTTCTGTCCCGATTTTACTCGAACTCCCCGTGTGTGGGGATGGAACGCGCTTTAAAACATCCTGTTCTTTTTCACTGACGGAGAGAAGCGACCGAACTCGGGTGATATCATGGAACTCCGGCTGGTTCa  <  2:3978624/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                
ACATGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCA  >  NZ_CP034943/2502076‑2502340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: