Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 177,053 C→A 23.2% intergenic (+226/‑46) EO946_RS01020 → / → EO946_RS01025 alcohol dehydrogenase/SulP family inorganic anion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,0530CA23.2% 318.6 / 40.0 210intergenic (+226/‑46)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base C (58/96);  new base A (53/2);  total (112/98)
Fisher's exact test for biased strand distribution p-value = 7.77e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTG  >  NZ_CP034943/176914‑177192
                                                                                                                                           |                                                                                                                                            
cTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                                                                              <  2:2289599/140‑1 (MQ=255)
cTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                                                                              <  1:3909232/140‑1 (MQ=255)
  tGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTTTCATTTATACCGTAAAATAAATAAATCTACCAAAacaa                                                                                                                                            >  1:4588661/1‑140 (MQ=255)
  tGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTATCCCGTAAAAAAAAGAAATCACACAATaaaa                                                                                                                                            >  1:3054524/1‑140 (MQ=255)
   gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAACACCCAAAaaaac                                                                                                                                           >  2:411206/1‑140 (MQ=255)
   gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTGATTTGTACCCTAAAAAAAAGAAAGCTCCCAAAacaaa                                                                                                                                           >  2:1868859/1‑139 (MQ=255)
   gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCAATTATCCCGTAAAATAAATAAAGCTCCCAATacaac                                                                                                                                           >  2:3248136/1‑140 (MQ=255)
   gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATCCCGTAAAATAAATAAAGCTCCCAAAaaaac                                                                                                                                           >  1:1237113/1‑140 (MQ=255)
   gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATCCCGTAAAATAAAGAAAGCTCCCAATacaaa                                                                                                                                           >  2:48497/1‑139 (MQ=255)
        tttGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTCTCCCGTAAAATAAAGAAAGCTCCCCAAACAAAAAATa                                                                                                                                      >  2:1985180/1‑140 (MQ=255)
        tttGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGCTCGTTTTAAATTAAATTAAAACGTAAAAAAAATAAAGATACCAATAAAACaaaaa                                                                                                                                      >  2:1249257/1‑138 (MQ=255)
             tCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTg                                                                                                                                 <  2:2640592/140‑1 (MQ=255)
                      aaTATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTTTGGCGGAAAAAAAATAAATCAGCCAATAGCAAAAATAAGCTGGGGgtttat                                                                                                                        >  1:3012218/1‑140 (MQ=255)
                             tACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTAATTTATACCCTAAAAAAAAGAAAAATCCCAAAAAAAAAAAAAAGGTGCGGATTTATTTTTTaa                                                                                                                 >  1:1603074/1‑140 (MQ=255)
                             tACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCAATTCTGCCGTAAAATAAAGGAATCTCCCAATACCACAAATAACGTGCGGATTTATTTTTTaa                                                                                                                 >  2:633167/1‑140 (MQ=255)
                                 ctTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAg                                                                                                             <  2:2397042/140‑1 (MQ=255)
                                  tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTTTACCGTAAAATAAAAAAAGATACCAATAAAACAAATAACGGGGGGGTTTATTTTTTtaaaatg                                                                                                            >  1:53249/1‑138 (MQ=255)
                                  tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAAGAAATCTCCCAAAACAACAAATAAGGTGCGGATTTATTTTTTAAAAAgg                                                                                                            >  2:2331916/1‑140 (MQ=255)
                                  tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTGATTTCTCCCGTAAAATAAAGAAAGCGACCAATACAAAAAAAACGGTGCGGATTTATTTTTTaaaaatg                                                                                                            >  1:3613857/1‑138 (MQ=255)
                                   ttGTATAATAACTCCTTGCCTCAAAACAACATACTCACCGTGTCCTCTTTTTCTCCGGGCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                                                                           <  2:4833848/140‑1 (MQ=255)
                                   ttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                                                                           <  2:917944/140‑1 (MQ=255)
                                    tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTACATTCTCCCGTAAAAAAAAGAAAGCTCACAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAgggg                                                                                                          >  2:2057784/1‑138 (MQ=255)
                                    tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTTTACCGTAAAATAAAAAAAACACCCCAAACAACAAAAAACGGGCGGATTTATTTTTTTAAAAagag                                                                                                          >  1:180002/1‑140 (MQ=255)
                                    tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCCAAAAATAAAGCAACCTCCCCATACAACCAAACAGCTGCGGATTTATTTTTTAAAAATGAg                                                                                                          >  2:406132/1‑140 (MQ=255)
                                       ataataACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTCAATTGTACCGTAAAATAAATAAAGCTCCCAAAAAAACAAATACCGTGCGGATTTATTTTTTaaaaatcagtgg                                                                                                       >  2:3798487/1‑133 (MQ=255)
                                       ataataACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCAGTTCTCCCGTAAAATAAATAAAACTCCCAATACAAAAAATACGGGGCGGATTTATTTTTTAAAAAGGAgggg                                                                                                       >  2:1680160/1‑137 (MQ=255)
                                        taataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGt                                                                                                      <  1:4298165/140‑1 (MQ=255)
                                          ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTAATTTATCCCCTAAAAAAAAGAAAGCTACCAAAAAAAAAAAAAACGGGCGGATTTATTTTTTAAAAAGTAGTCGTta                                                                                                    >  1:2310104/1‑140 (MQ=255)
                                          ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAAATTATCCCGGAAAATAAATAAAGCTCCCAATAAAACAAATACGGTGCGGATTTATTTTTTAAAAATGAGTCGTta                                                                                                    >  2:1230370/1‑140 (MQ=255)
                                          ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCGTTCATTTCTCCCCTAAAATAAAGAAATCTCCCAAAACAACAAATACGGTGCGGAGTTATTTTTTAAAAAGGAGTCGtgt                                                                                                    >  2:2366396/1‑138 (MQ=255)
                                          ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACATTCATTTCTACCCTAAAATAAAGAAAGCTCCCAAAACAAAAAATAAGGTTCGGATTTATTTTTTAAAAAGGAGTCGTta                                                                                                    >  2:3577400/1‑140 (MQ=255)
                                           taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTCTCCCGTAAAATAAAGAAATCTCCCAAAAAAACAAATAAGGGGCGGATTTATTTTTTTAAAAGGGGTAGTtat                                                                                                   >  2:1796219/1‑140 (MQ=255)
                                           taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTGTGCCGTAAAATAAATTAATCTCCCAATAAAACAAAAACGGTGCGGGTTTATTTTTTAAAAATGTGTCGTtat                                                                                                   >  2:3657656/1‑140 (MQ=255)
                                           taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATATCTCCCCTAAAATAAAGAAAGCTCCCAATACAAAAAATACGGTGCGGAATTATTTTTTAAAAAGGAGTCCTtat                                                                                                   >  2:247327/1‑140 (MQ=255)
                                            aaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTATCCCCTAAAAAAAAGAAACCTCCCAAAAAAACAAAAAACGTGCGGGGTTATTTTTTAAAAATGAGTCGTtatt                                                                                                  >  1:2887975/1‑139 (MQ=255)
                                            aaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTCATTTGTCCCGTAAAAAAAATAAAAATACCAAAACAACAAAAAAGGTGGGGATTTATTTTTTAAAAAGGTGGCGttgtt                                                                                                  >  1:2347284/1‑137 (MQ=255)
                                             acaccTTGCCTCAAAACAAAATAATCAAGGTGTCCTCTTTTTATCCGGTCGTTTTCCTTTCATTTCTCCAGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                 <  1:1864930/137‑1 (MQ=255)
                                             aCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                 <  1:48497/140‑1 (MQ=255)
                                             aCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAAGAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                 <  1:1716056/140‑1 (MQ=255)
                                              cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTAATTTCTACCGTAAAATAAAGAAAGCTCCCAATAAAACAAATACGGGGGGGGTTTATTTTTTAAAAAGGGGTCGTTTTAtt                                                                                                >  2:49832/1‑140 (MQ=255)
                                              cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCATTCATATCTCCCGTAAAAAAAATAAAACACCAAATACAAAAAAAAAAGTGGGGAGTTATTTTTTAAAAAATATTGGTTATAtt                                                                                                >  1:4688335/1‑140 (MQ=255)
                                              cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTGATTTATACCCTAAAAAAAAAAAAAAACCCAAAAAAAAAAAAAACGTTCGGGTTTATTTTTTAAAAAGTAGGCGGGATAtt                                                                                                >  1:1906118/1‑140 (MQ=255)
                                                ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTTTGATTTGTGCCGTAAAATAAAGAAAGCTGCCCATAAAACAAATAAGGGGCGGTGTTATTTTTTATAAAGGAGTCGGTGTAtttt                                                                                              >  1:2795555/1‑139 (MQ=255)
                                                ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTGTACCGTAAAATAAAGAAACCTCCCAAAAAAACAAAAAGGGTGCGGATTTAAGTTTTAAAAAGGAGGCGTGATAATTg                                                                                              >  2:4582338/1‑140 (MQ=255)
                                                ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAATTTCTCCCGGACAAAAAAGCAAAGTCCCAAAACAACAAAAACGGTGAGGCTTTGTTTTTTAAAAAGGTCTCGGTTTAtttt                                                                                              >  1:2959567/1‑139 (MQ=255)
                                                ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTGAATTATCCCCTAAAATAAACAAAGCACCCAATACAAAAAAAACGGTGCGGGTTTATTTTTTAAAAAGTCGTAGTTATATTTg                                                                                              >  2:4478991/1‑140 (MQ=255)
                                                   tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCGTTGATTTCTACCCTAAAATAAATAAAGCTCCCAATACAACAAAAAAGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATAATTGaat                                                                                           >  1:3654231/1‑140 (MQ=255)
                                                   tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTGTCCCGGAAAAATAATAAAGCTCCCCATACAACACAAACGGGGGGGGTTTATTTTTTTAGGAGGAGTGGGTTTAttttgtt                                                                                           >  1:3465984/1‑136 (MQ=255)
                                                     ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                         <  2:3096569/140‑1 (MQ=255)
                                                     ccTCAAAACAACAGACTCAACGTGTCCTCTTTTTCTCCGGTCTTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                         <  2:3468422/140‑1 (MQ=255)
                                                           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                  <  1:2017786/140‑1 (MQ=255)
                                                           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                  <  1:3441661/140‑1 (MQ=255)
                                                           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                  <  1:260981/140‑1 (MQ=255)
                                                           aacaacATACTCAACGGGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTACCTTAAAATAAAGAAAGCTCCCAATACAACAAATACTTTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                  <  2:2222902/140‑1 (MQ=255)
                                                             caacaTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTa                                                                                <  2:180002/140‑1 (MQ=255)
                                                                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTGTCCCGTAAAATAAAGAAAGCTCCCAATACCACAAATACGTTGCGGATTTATTTTTTAAAAAGGAGTCGTTATAATTGTATAATAGtttttgat                                                                             >  2:4856586/1‑136 (MQ=255)
                                                                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAATACGGTGCGGATTTATTTTTTTAAAATGAGTGGGTATATTTGAATTATAGTTTTAGAt                                                                             >  2:4706896/1‑140 (MQ=255)
                                                                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAATAAGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGTATAATAGTTTTACAt                                                                             >  2:3369716/1‑140 (MQ=255)
                                                                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTTCGTTGATTTGTGCCGTAAAATAAATAAAACTCCCCAAACAAAAAAAAAGGGGGGGGTTTATTTTTTTAAAATGAGTGGGTTTTTTTTTATAATATGttttgaa                                                                              >  1:2331770/1‑137 (MQ=255)
                                                                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCGTTGATTTGTCCCGTAAAAAAAAGAAAGCTCCCAAAACAACAAAAAAGGGGCGGATTTAATTTTTAAAAATGAGTGGTTATATTTGTATAATATTTTTaaaa                                                                             >  1:2375842/1‑137 (MQ=255)
                                                                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGAATTGTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATAAGGTGGGGATTTATTTTTTAAAAAGGAGGGGTTATATTTTAATAATATTTTTAGATTaa                                                                          >  2:4263892/1‑140 (MQ=255)
                                                                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACGTTaa                                                                          <  2:4414828/140‑1 (MQ=255)
                                                                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTaa                                                                          <  2:445480/140‑1 (MQ=255)
                                                                   aCTCAACGTGTCCTCTTGTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTaa                                                                          <  2:1149468/140‑1 (MQ=255)
                                                                     tCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAag                                                                        <  1:3657656/140‑1 (MQ=255)
                                                                       aaCGTGTCCTCTTTTTCTCCGGTCGTGTTGGGTTGATTTGTCCCGTAAAAAAAAGAAAGCACCCAAAACAAAAAAAAAGGGGGGGGTTTATTTTTTTAAAAGGAGTGGTTTTTTTTTTAATATATTTTTAAAATAAaaca                                                                      >  1:4213431/1‑140 (MQ=255)
                                                                        aCGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGTTTTATTTTTTTAAAAGGAGTCGTTATATTTTAATAATAGTTTTACAATAAagcag                                                                     >  2:2452231/1‑140 (MQ=255)
                                                                        aCGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGGAAAAAAAAAAAAGCAGCCAAAACAAAAAATAAGGGGCGGGTTTATTTTTTAAAAAGGAGTCGGTGGATTTTTGTAATATTTTTTAATTAAagcag                                                                     >  1:968266/1‑140 (MQ=255)
                                                                        aCGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTTTCCCGGAAAAAAAAGTAAGCTACCAAAAAAAAAAAAAACGGGGGGGTTTTTTTTTTAAAAAGGATTGGGTTTTTTTGTATAAAAATTTTTAATTTAatcag                                                                     >  1:2125232/1‑140 (MQ=255)
                                                                          gtgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAgcagca                                                                   <  1:4538630/140‑1 (MQ=255)
                                                                          gtgtCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTATCCCCTAAAATAAAGAAAGCAACCAAAAAAACAAATACGGTGCGGATTTAATTTTTAAAAAGGGGTGGGTATATTTTAATAATATGTTTACATTAAAgcatca                                                                   >  2:1009051/1‑140 (MQ=255)
                                                                           tttCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                  <  2:2409809/138‑1 (MQ=255)
                                                                           tgtCCTCTTTTTCTGCGGTCGTTTTCCTTTCATTTCTCCATTAAAATATAGAAATCTCCCAATAAAACAAAGACTGTGCTGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                  <  2:4655913/140‑1 (MQ=255)
                                                                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                  <  2:132328/140‑1 (MQ=255)
                                                                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                  <  2:1356580/140‑1 (MQ=255)
                                                                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                  <  2:1239308/140‑1 (MQ=255)
                                                                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                  <  2:1082486/140‑1 (MQ=255)
                                                                            gtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAAt                                                                 <  1:4104564/140‑1 (MQ=255)
                                                                             tCCTCTTTTTCTCCGGTCGTTTTGCGTTGATTTGTGCCCTAAAAAAAATAAAGCTCCCCAAAAAACAAAAAACGGGGGGCTTTATTTTTTTAAAAAGAGGGGGTATATTTTTATAAAATTTTTAAAATAAATcaacaaag                                                                >  1:3825500/1‑138 (MQ=255)
                                                                             tCCTCTTTTTCTCCGGTCGTTTTACTTTGAATTCTACCCAAAAATAAAATAAGCTCCCAAAAAAACAAATTCGGTGCGGGATTATTTTTTAAAAAGTAGTCGTTATATGTGTATAAAAGTTTTTAAATAAAGAACCaaag                                                                >  2:806196/1‑138 (MQ=255)
                                                                                       ctcCGGTCGTTTTCCTTTAATTTCTCCCGTAAAATAAAGAAAGCTGCCAAAACAACAAAAAAGGGGCGGAGTTATTTTTTAAAAATCTGTCGTTTTTTTTTAATAATAGTTTTACATTAAATCAGGAAATGTTAGGGAat                                                      >  2:4159574/1‑140 (MQ=255)
                                                                                         cctgTCGTTTTCCTTCGATTTCTCCCGTAAAATACAGAAATCTCGCACTACAACAAATACGGTTTGGATTTATTTTTTAACAAGGATTCGTTATATTTTAATAATAGTTTTACATTAAAGCAGGAATGGTTCGGGAatat                                                    <  2:1454456/137‑1 (MQ=255)
                                                                                         ccGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACCACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAatat                                                    <  2:4773547/140‑1 (MQ=255)
                                                                                         ccGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAatat                                                    <  2:3388743/140‑1 (MQ=255)
                                                                                         ccGGTCGTTTTCCTTTCATTTCTCCCGTAAAAGAAAGAAAGCTCCCAATACCACAAATACGGTGCGTATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAatat                                                    <  2:331836/140‑1 (MQ=255)
                                                                                            gTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCg                                                 <  2:2869538/140‑1 (MQ=255)
                                                                                                         cATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCtt                                    >  1:1141485/1‑140 (MQ=255)
                                                                                                                      aaaTAAAGAAAGCTCCCAATACCACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                       <  1:1249394/140‑1 (MQ=255)
                                                                                                                      aaaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                       <  1:3248136/140‑1 (MQ=255)
                                                                                                                       aaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGt                      <  1:3530557/140‑1 (MQ=255)
                                                                                                                            agaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt                 <  1:379757/140‑1 (MQ=255)
                                                                                                                             gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                <  1:2452231/140‑1 (MQ=255)
                                                                                                                             gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                <  1:1944668/140‑1 (MQ=255)
                                                                                                                             gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                <  2:833145/140‑1 (MQ=255)
                                                                                                                             gaaacCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                <  2:239035/140‑1 (MQ=255)
                                                                                                                              aaagCTCCCAATACCACAAATACGGTGCGGATTTAATTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTa               <  1:1083172/140‑1 (MQ=255)
                                                                                                                              aaagCTCCCAATACAACAACTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTa               <  1:4115706/140‑1 (MQ=255)
                                                                                                                                  cTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAt           >  2:372027/1‑140 (MQ=255)
                                                                                                                                    cccAATACAACAACTCCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa         <  2:2583821/140‑1 (MQ=255)
                                                                                                                                           caacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg  <  1:716081/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTG  >  NZ_CP034943/176914‑177192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: