Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,680,491 A→C 6.3% F387V (TTC→GTC)  EO946_RS13875 ← LysM peptidoglycan‑binding domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,680,4910AC 6.3% 631.3 / 3.0 244F387V (TTC→GTC) EO946_RS13875LysM peptidoglycan‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (103/111);  new base C (7/20);  total (110/134)
Fisher's exact test for biased strand distribution p-value = 3.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

TGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTCC  >  NZ_CP034943/2680352‑2680627
                                                                                                                                           |                                                                                                                                        
  cTCCGGTTGAGCCGCTTCTTTCTGTTCTTGACGGTGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTGCTTCTGGTTCTTGTAGGAGTGCGTGCTCGCCGTGCTCCGGTTGTGCGGCTTCTTCTGATTCTGGGCGGAATg                                                                                                                                        >  2:785262/1‑140 (MQ=17)
  cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                                                                                                        <  1:51362/140‑1 (MQ=255)
  cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTGCTTCTGCTTCTTGACGGAATg                                                                                                                                        >  2:4392177/1‑140 (MQ=255)
  cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCGTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                                                                                                        >  1:1315297/1‑140 (MQ=255)
   tCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGc                                                                                                                                       <  2:3065201/140‑1 (MQ=255)
   tCCGGTTGAGCCGCTTCTTTCTCTTCTGGACGGAGTGCGGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGc                                                                                                                                       <  2:1708648/140‑1 (MQ=255)
       gTTGCGCCGCTTCTGACTCTTCTTGACGGAGTGCGGCCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGc                                                                                                                                   <  2:3304488/140‑1 (MQ=255)
       gTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGTGGTTCTTGGCGGAATGCAGGc                                                                                                                                   >  2:3131412/1‑140 (MQ=255)
       gTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCCGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCAGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGc                                                                                                                                   <  2:2530798/140‑1 (MQ=255)
          gAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCa                                                                                                                                <  1:3366818/140‑1 (MQ=255)
          gAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCa                                                                                                                                >  2:3772356/1‑140 (MQ=255)
          gAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCATCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCa                                                                                                                                <  1:776627/140‑1 (MQ=255)
           aGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCGTCTTCAGCTTCTTGACGGCGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCATGTTCTTGATGACGGTATTCAGGCTCAg                                                                                                                               >  1:528092/1‑140 (MQ=14)
             ccGCTTCTTTCTCTTCTTGACGGAGTGCTGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGAGGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCTCTTCTTAGTCTTCTTGAGCGAATGCAGGCACAGCg                                                                                                                             >  2:2363566/1‑140 (MQ=14)
             ccGCGTCTTTCTCTTCGTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCGTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                                                                                                             >  1:2884578/1‑140 (MQ=255)
               gCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCAGCTTAAAAATATTCTAGAAGGAAAGCACGCTCAGCGTg                                                                                                                           >  2:561959/1‑140 (MQ=14)
                     ttctCTTGTTGACGCAGTGCTGGCTCATTGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCgg                                                                                                                     <  1:4530843/140‑1 (MQ=17)
                      tctcTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGt                                                                                                                    <  1:764489/140‑1 (MQ=255)
                          ttcttGACGGAGTGCGGGCTCAACTTGCTCCGGTTGAGCCTCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAg                                                                                                                <  1:3860934/140‑1 (MQ=255)
                                  ggAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGcttct                                                                                                        >  2:3583705/1‑140 (MQ=255)
                                    aGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt                                                                                                      <  1:4699694/140‑1 (MQ=255)
                                    aGTGCGGGCTCAACGTGCTCCGGTTTAGCCGCGTCTTCTGCTTGTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGAGGGACTGCAGTCTCAGCGTGCGCCGGTTGAGCTGCgtcttt                                                                                                      >  1:475030/1‑140 (MQ=17)
                                    aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTTACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt                                                                                                      >  1:601440/1‑140 (MQ=255)
                                    aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt                                                                                                      >  2:354767/1‑140 (MQ=255)
                                           gCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCtcttct                                                                                               <  1:4274483/140‑1 (MQ=255)
                                              cAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGa                                                                                            >  1:280028/1‑140 (MQ=255)
                                              cAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGa                                                                                            <  1:3212814/140‑1 (MQ=255)
                                              cAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCATCTTCTGCTTCATGACGGAATGCAGGCTCAGCGTGCTCCCGTTGAGCTGCTTCTGGCTcttcttta                                                                                            >  2:3680343/1‑138 (MQ=17)
                                              cAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCGGCTTCATTCtgttgatca                                                                                            >  1:2394815/1‑135 (MQ=17)
                                              cAACGTGCTCCGGTTGAGCCGCTTCTTCGGCTTCTTGCCGGAGTGCGGGCTCAGCGTGCTCCGGGTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCGCAGCGTGCTCCGGTTGAGCAGCTGCGTTCTCTGCTGGa                                                                                            >  1:2109514/1‑140 (MQ=14)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGCGTGCGGGCTCAGCGTGCTGCGGTTGAGCCGGTTCTTCTGGTTCTTGACGGAATGGAGGCTCAGCGTGCTCCTGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                          >  1:4363945/1‑140 (MQ=17)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                          >  2:2451759/1‑140 (MQ=255)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                                                                                                             <  2:1478927/105‑1 (MQ=255)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                                                                                                             <  2:257631/105‑1 (MQ=255)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTcttcttcaag                                                                                          >  2:753597/1‑136 (MQ=255)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                          <  1:4058091/140‑1 (MQ=255)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                          <  1:3453693/140‑1 (MQ=255)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTATCTTCTTGACg                                                                                          >  2:288210/1‑140 (MQ=255)
                                                aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTGCTTCGGCTTCTTGACGGACTGCGGGCTCAGCGTGCGCCGGTTGAGCGGCTTCTTTCTCTTGTTGGCg                                                                                          >  2:269126/1‑140 (MQ=14)
                                                     cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                                                                     <  2:1912766/140‑1 (MQ=255)
                                                     cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                                                                     <  2:4690779/140‑1 (MQ=255)
                                                     cTCCGGTTGAGCCGCTTCTTCGGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                                                                     <  2:203150/140‑1 (MQ=255)
                                                     cTCCGGTTGAGCCGCTTCGTCTGCTTCTTGACCGAGTGCGGGCTCAGCGTGGTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTACTCTTCTTGACGGAATg                                                                                     <  2:4895086/140‑1 (MQ=255)
                                                       ccGGTTGCGCCGCTTCTTCAGCTTCTGGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCa                                                                                   <  2:2740548/140‑1 (MQ=255)
                                                       ccGCTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCa                                                                                   <  2:3072514/140‑1 (MQ=255)
                                                              aGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAg                                                                            <  1:3100441/140‑1 (MQ=255)
                                                                ccGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCg                                                                          <  1:71722/140‑1 (MQ=255)
                                                                             ttcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGATTGCAGGCTCAGCGTCTTCCGGTTGAg                                                             <  1:63193/140‑1 (MQ=255)
                                                                             ttcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCAGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAg                                                             <  2:2194588/140‑1 (MQ=255)
                                                                             ttcatcaCGGAGCGCGGGCTCAGCCTGCTCCCGTTGAGCCGCTTCTTCAGCTTCTTGACGGACCGCAGGCTCAGCGTGCTCCGGTTGAGCAGCTTCTTTCTCTTCTTGACGGACTGCAGGCTCAGCGTCTTCCGGTTGAg                                                             <  2:1978073/134‑1 (MQ=14)
                                                                              tcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgc                                                            >  2:4092592/1‑140 (MQ=255)
                                                                              tcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgc                                                            >  2:458520/1‑140 (MQ=255)
                                                                              tcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgc                                                            >  1:3013893/1‑140 (MQ=255)
                                                                                 tGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgctgc                                                         >  2:2011848/1‑140 (MQ=255)
                                                                                 tGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTATTCTGCTTCTTGACGGAATGCAGGCTCATCGTGCTCCGGTTGAGATGCTTCTTTCTCTTCTTGTCGGAATGCAGGATCAGCGTATTCCTGTTGAgctgc                                                         >  1:1727210/1‑140 (MQ=17)
                                                                                  gACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgctgct                                                        >  1:4312307/1‑140 (MQ=255)
                                                                                     ggAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGcttct                                                     <  1:458520/140‑1 (MQ=255)
                                                                                     ggAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGcttct                                                     <  1:3740844/140‑1 (MQ=255)
                                                                                       aGTGCGGGCTCAGCGTGCTCCGTTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttcttt                                                   <  2:62745/140‑1 (MQ=255)
                                                                                       aGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttcttt                                                   <  2:4286213/140‑1 (MQ=255)
                                                                                       aGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttcttt                                                   <  2:47987/140‑1 (MQ=255)
                                                                                       aGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttcttt                                                   >  1:3130211/1‑140 (MQ=255)
                                                                                        gTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttctttc                                                  >  1:1410086/1‑140 (MQ=255)
                                                                                         tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttctttct                                                                                                    >  2:4222292/1‑89 (MQ=255)
                                                                                         tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttctttct                                                 >  1:1820202/1‑140 (MQ=255)
                                                                                            gggCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCtctt                                              >  1:4939955/1‑140 (MQ=255)
                                                                                              gCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCtcttct                                            <  1:243496/140‑1 (MQ=255)
                                                                                              gCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCtcttct                                            <  1:21301/140‑1 (MQ=255)
                                                                                                 cAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTGTTGa                                         >  2:3802158/1‑140 (MQ=255)
                                                                                                  aGCGCGCTCCGGTTGCGCCGGTTATTCGGCTTCATGACGCAGTGCAGGCTCAGCGTGGTCCGGTTGAGCTGCTTCTTTCGCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGAc                                        <  2:2221856/140‑1 (MQ=14)
                                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACTGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                       >  1:2598178/1‑140 (MQ=255)
                                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTGTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCGTCTTGagg                                       >  1:158527/1‑138 (MQ=255)
                                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGACTGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCGTTCTCTGCTTGACg                                       >  2:1961846/1‑140 (MQ=255)
                                                                                                   gCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCGTGACGGAATGCCGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTATTCTTGaag                                       >  1:1886472/1‑138 (MQ=255)
                                                                                                    cGTGCTCCGGTTGAGCCGCTTCTTTCGCTTCTGGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACgg                                      <  2:1534422/140‑1 (MQ=255)
                                                                                                    cGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACgg                                      <  2:1116020/140‑1 (MQ=255)
                                                                                                        cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACTGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                  <  2:2598178/140‑1 (MQ=255)
                                                                                                        cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                  <  1:2760164/140‑1 (MQ=255)
                                                                                                        cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGTCTCAGCGAGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                  <  2:2946072/140‑1 (MQ=255)
                                                                                                        cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                  >  1:4171012/1‑140 (MQ=255)
                                                                                                        cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                  <  2:1315297/140‑1 (MQ=255)
                                                                                                        cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                  <  1:1524792/140‑1 (MQ=255)
                                                                                                        cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                  <  1:3583866/140‑1 (MQ=255)
                                                                                                             gTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGc                             <  2:1302602/140‑1 (MQ=255)
                                                                                                             gTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGc                             <  1:1235144/140‑1 (MQ=255)
                                                                                                             gTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGc                             <  2:4564339/140‑1 (MQ=255)
                                                                                                             gTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGc                             <  1:3562547/140‑1 (MQ=255)
                                                                                                              ttGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGc                                                                                                                          >  2:3438848/1‑46 (MQ=255)
                                                                                                              ttGAGCCGCTTCTTCTGCGTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCt                            >  1:883458/1‑140 (MQ=255)
                                                                                                                gAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCa                          <  2:3640579/140‑1 (MQ=255)
                                                                                                                 aGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAg                         >  1:2307495/1‑140 (MQ=255)
                                                                                                                  gCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGc                        <  1:3292435/140‑1 (MQ=255)
                                                                                                                   ccGGTTCTTTTGCTTCTTGAAGCACTGCAGGGTCAGCGTGCTCCGGTTGAGCGGCTTCTTTCTCGTCTTGACGGACTGCAGGCTCAGCGTGTTCGGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCg                       <  2:848095/140‑1 (MQ=14)
                                                                                                                   ccGCTTCTTCTGCTTCTTGACGGTCTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGACTGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCg                       <  2:350694/140‑1 (MQ=255)
                                                                                                                        tcttctGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTc                  >  2:1001956/1‑140 (MQ=255)
                                                                                                                            ctGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCATGACGGAATGCAGGCTCAGCGTGCTCCGGt              >  2:3425953/1‑140 (MQ=255)
                                                                                                                                 tcttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGc         >  2:4518586/1‑140 (MQ=255)
                                                                                                                                 tcttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGc         >  2:740580/1‑140 (MQ=255)
                                                                                                                                   ttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCg       <  2:4939955/140‑1 (MQ=255)
                                                                                                                                   ttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCg       <  1:1001956/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTCC  >  NZ_CP034943/2680352‑2680627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: