Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,812,790 T→G 8.4% E199D (GAA→GAC EO946_RS14520 ← SDR family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,812,7900TG 8.4% 533.4 / 3.7 264E199D (GAA→GACEO946_RS14520SDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (117/106);  new base G (34/3);  total (154/110)
Fisher's exact test for biased strand distribution p-value = 2.85e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGCCAAATG  >  NZ_CP034943/2812651‑2812926
                                                                                                                                           |                                                                                                                                         
gaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCt                                                                                                                                           <  2:243871/140‑1 (MQ=255)
gaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCt                                                                                                                                           <  2:2787925/140‑1 (MQ=255)
  ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATGCGGGCGATATCCTCGCCTGTGCCAGATATTCCTATCGGCGTTTTTTCCTTGACGATTCGCTTCCTGGCTTc                                                                                                                                         <  2:724199/140‑1 (MQ=255)
  ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                         <  2:4820309/140‑1 (MQ=255)
  ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                         <  1:2292813/140‑1 (MQ=255)
  ccTCCGGTGGCTTCAAGGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCATATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                         <  1:926245/140‑1 (MQ=255)
   cTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACCCAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTcc                                                                                                                                        <  2:268477/140‑1 (MQ=255)
    tCCGGTGGATTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCct                                                                                                                                       <  1:3605179/140‑1 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCctc                                                                                                                                      >  1:1013868/1‑140 (MQ=255)
      cGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCctct                                                                                                                                     >  2:3161870/1‑140 (MQ=255)
           gCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGa                                                                                                                                <  2:1083489/140‑1 (MQ=255)
           gCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTGCCGAGTCGCTTGCTGGCTTCCTCTAGTgt                                                                                                                                >  1:4677402/1‑139 (MQ=255)
             ttCACTGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCGTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                              <  2:2941966/140‑1 (MQ=255)
             ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGGGTCCTCTATTGATg                                                                                                                              >  2:3054417/1‑140 (MQ=255)
             ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                              <  2:1791504/140‑1 (MQ=255)
             ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                              <  2:1567338/140‑1 (MQ=255)
             ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                              <  1:4497002/140‑1 (MQ=255)
             ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGGCGATTCGCGTCCTGGCGTCCTCTATTGATg                                                                                                                              >  1:2124398/1‑140 (MQ=255)
             ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCAGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACGTGCCGATTCGCTTCCTGGCGTCCGCACTTGATg                                                                                                                              >  1:9634/1‑140 (MQ=255)
              tCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGc                                                                                                                             <  1:3801005/140‑1 (MQ=255)
                aaTGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCTTCCTCTATTGATGctt                                                                                                                           >  2:2571291/1‑140 (MQ=255)
                      aGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGGTTCCTGTATTGATGGTTCCTTCa                                                                                                                     >  2:1895520/1‑140 (MQ=255)
                       gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCGTTACGCTTAGCTTCCTTGCGTCCGCTAATGATTGTTCCTTAAt                                                                                                                    >  1:1636724/1‑140 (MQ=255)
                       gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGCTTCGCTTCCTTGCGTCCTCTATTGATGCGTCCTTGAt                                                                                                                    >  2:804170/1‑140 (MQ=255)
                       gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTAGCTTCCTTGCGTCCGCTATTGATGCTTCCTTAAt                                                                                                                    >  2:4384482/1‑140 (MQ=255)
                          ccTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTc                                                                                                                 >  2:422819/1‑140 (MQ=255)
                                acaAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTGCCGATTCGCTTCCTGGCTTCCGCTATTGATGCTTCCTTCATGTCGCCCAc                                                                                                           >  2:2695408/1‑140 (MQ=255)
                                 caAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACa                                                                                                          >  2:4700325/1‑140 (MQ=255)
                                   aGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAt                                                                                                        <  2:1620428/140‑1 (MQ=255)
                                    ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCAAGTCGCCGACAATg                                                                                                       >  2:4434049/1‑140 (MQ=255)
                                    ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCAAGTCGCCGACAATg                                                                                                       >  2:259542/1‑140 (MQ=255)
                                    ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTTATGCGTCCTTCATGTCGCCGACACTg                                                                                                       >  2:1242234/1‑140 (MQ=255)
                                        tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTACTGGCTTCCTCTAGTGATGCTTCCTTCATGTCGCCGACACTGTCAc                                                                                                  >  2:3890249/1‑140 (MQ=255)
                                        tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCGTCTATTGAAGCTTGCTTCATGTCGCCGACCATGTGAc                                                                                                  >  2:3932878/1‑140 (MQ=255)
                                        tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACACTGTCAc                                                                                                  >  2:2381544/1‑140 (MQ=255)
                                        tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGCCGATTCGCGTCCTGGCGTCCGCTATTGATGCTTCCGTCATTTCGGCGACAAAGTCAc                                                                                                  >  1:484461/1‑140 (MQ=255)
                                         gAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCATATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTATATTGATGCTTCCTTCATGTCGCCGACAATGTCAcc                                                                                                 <  1:4983246/140‑1 (MQ=255)
                                         gAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCAcc                                                                                                 <  1:275759/140‑1 (MQ=255)
                                         gAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCAcc                                                                                                 <  1:3412567/140‑1 (MQ=255)
                                         gAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCAcc                                                                                                 <  1:1779122/140‑1 (MQ=255)
                                             tctcCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCggg                                                                                             <  1:3161870/140‑1 (MQ=255)
                                             tctcCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCggg                                                                                             <  1:681718/140‑1 (MQ=255)
                                              ctcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGc                                                                                            <  1:1867098/140‑1 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTGCTTCATGTCGCCGACAAAGTCACCCCGGCa                                                                                           >  1:1351607/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGGTTCCTCTATTGATGCTTGCTTCATGTCGCCGACAATGTCACCCGGGCa                                                                                           >  2:1975991/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCa                                                                                           >  2:775160/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCGGGGTTCCTCTATTGATGGTTCCTTCATGTCGCCGACAATGTCCCCCGGGCa                                                                                           >  2:643858/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTAGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAAGTCACCCGGGCa                                                                                           >  2:1297909/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGCTTCGCTTCCTGGCTTCCGCTATTGATGGTTCCTTCATGTCGCCGACACTGTCACCCGGGCa                                                                                           >  2:8580/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGAAATCCTCGCCTGTGCCAGATCGTCCTATCGGCGTTGAGTCCTTGCCGATTCGCTTACTGGGTACCTCTATTGATGCTTACTTCATGTCGCCGACAAATTCACCCGGGCa                                                                                           >  2:4777819/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCTATAATCCGGGCGATATCATCGCATGTGCCAGATCTTCCTATCGGCGTTTTTTACATGCCCAATCGCGTCCTGGCTTCCGCTAGTGATGCTTAGATCATGTCACCGACAAAGTCCCCCGGGCaa                                                                                          >  2:1672326/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTGCTTGGTTGCTCTAATGATGCTTCCTTGAGGTCGCCGACCATGTGCACCggggac                                                                                           >  2:2882909/1‑138 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCGTCCTGGCGTCCTCTATTTATGCGTCCTTAATTTAGCCCACAAAGTCACCCCGGcca                                                                                          >  1:2839337/1‑138 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCaa                                                                                          >  2:220895/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGTTTCGCTTCCTGGGGTCCGGTATTGATGCTGCCTTCATGTCGCCGACAATGTCACCCGGGcca                                                                                          >  2:4177106/1‑138 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCaa                                                                                          >  2:4912974/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCaa                                                                                          >  2:3587381/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCATCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCaa                                                                                          >  2:1744047/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCaa                                                                                          >  2:2284634/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTGTATTGTTGCTTCCGTCATGTCGCCGACAATGTCACCCGGGCaaa                                                                                         >  2:4097045/1‑140 (MQ=255)
                                                    acacaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTTCCGATTAGCTTCCTGGCTTCCTCTATTGATGGTTGCTTAAAGTGGGCGACACTGTGACCCGGGCAAACCa                                                                                      >  1:2946831/1‑140 (MQ=255)
                                                    acacaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGGCGATTCGCTTCCTGGCTTGCTGGATTGATGGTTCCTTCATTTAGCCGACAAAGTGACCCCGGCCAACCa                                                                                      >  1:199731/1‑140 (MQ=255)
                                                        aaGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGtt                                                                                  <  1:2310984/140‑1 (MQ=255)
                                                        aaGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGtt                                                                                  <  1:271743/140‑1 (MQ=255)
                                                         aGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAAGTCACCCGGGCAAACCATGTTg                                                                                 >  1:4870166/1‑140 (MQ=255)
                                                             aGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCAc                                                                                                  <  1:1107431/119‑1 (MQ=255)
                                                             aGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCCTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAg                                                                             <  1:8559/140‑1 (MQ=255)
                                                                  tAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATg                                                                        <  1:440045/140‑1 (MQ=255)
                                                                    aTCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGcc                                                                      <  2:45889/140‑1 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTAGCTTCCTGGCTTCCTCTATTGTTTGTTCCGTCATTTCGCCGACAAAGTCACCCCGGCCAAACAGGTTGTCACGTATTCCGaaat                                                                 >  1:30133/1‑138 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGGTTCCTGGGTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCCCCCGGGCAAACCATGTTGGCAGTTATGCCGAAtt                                                                 >  2:1358025/1‑140 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGGGTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGaaat                                                                 >  2:2998271/1‑138 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCTTCCTGTATTGTTGCTTCCTTCATGGCGCCGACAAAGTCACCCGGGCCAACCCGGGTTGCCGGGATGCCGAAtt                                                                 >  2:370491/1‑140 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTAGCGTCCTGGCGTCCTCTATTGATGCTTCCTTCAAGTCGCCGACAAAGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAAtt                                                                 >  2:1323274/1‑140 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCCATTCGCTTCCTGGCGTGCTCTATTGATGCTTCCTTCAAGTCGCCGACAAAGTCACCCCGGCCAACCAGGTTGGCAGTTATTCCGaaat                                                                 >  1:4331135/1‑138 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGGCGAATCGCGTCCAGGCTTCCTCTATTTTTGCTTCCTTCATGTCGCCGACAATGTAACCCCGGCCAACCAAGGTGGGCGTTATTCCCaaaa                                                                 >  1:1978950/1‑138 (MQ=255)
                                                                             atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGTTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                             <  1:3306862/140‑1 (MQ=255)
                                                                             atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                             <  1:733190/140‑1 (MQ=255)
                                                                              tatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTGCTGCATGTCGCCGCCAATGTCACCCGGGCAAACCATGTTGGCGGTTTTGCCGAATTCGGct                                                            >  2:2345855/1‑140 (MQ=255)
                                                                                   tCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCttcttc                                                       <  1:4028093/140‑1 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCCATTCGCTTCCGGGGTTCCTCTATTGATGCTTACTTCATGTCGCCCACAAAGTGACCCCGGCAAACAAAGTTGGCAGTTATTCCGAATTCGGCGTCTTaaatggcc                                                >  2:1628477/1‑139 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCGCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCCGGCAAACCAGGTTGGCAGGTATGCCGAATTCGGCGTCGTcaatggca                                                >  2:2167141/1‑140 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCGTCCTCTATTGATTCGTCCTTCATGTCGCCGACAAAGTCACCCGGGCAAACCATGTTGGCAGTTATTCCGAATTCGGCTTCTTcaatggca                                                >  2:58467/1‑140 (MQ=255)
                                                                                           gCCAGATCTTCCTATCGGCGTTTTTTCCTGGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                               <  2:3238721/140‑1 (MQ=255)
                                                                                               gATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGt                                           <  2:2009506/140‑1 (MQ=255)
                                                                                                 tCTTCCTTTCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCt                                         <  2:3358823/140‑1 (MQ=255)
                                                                                                    tCCTATCGGCGTTTTTTCCTTGCCGCTTCGCGTCCTGGCTTCCTCTATTGATGCTTCCGTCATTTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCg                                      >  2:3750778/1‑140 (MQ=255)
                                                                                                    tCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCGTCATGTCCCCGACAAAGTCACCCGGGCAAACCCTGTTGGCAGTTATGCCGAATTCGGCGTCTTCAATGGCAAGGGTCTTCg                                      >  2:3481594/1‑140 (MQ=255)
                                                                                                    tCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTAATGATGCTTCCTTCATGTCGCCGACAATGTCCCCCGGGCAAACCAGGTTGGGAGTTATGCCGACTTCGGCTTCTTCAATGGCAATGGTGTTCg                                      >  2:2646385/1‑140 (MQ=255)
                                                                                                    tCCTATCGGCGTTTTTTCCTTGCCGATTAGCGTCCTGGCTTACTCAATTGATGCTTCCTTAATTTCGCCCACAATGTCACCCGGGCAAAACATGTTGGCAGTTATTCCGAATTAGGCTTGTTCAAAGGCAATGGTATTCg                                      >  2:4529990/1‑140 (MQ=255)
                                                                                                    tCCTATCGGCGTTTTTTCCTTGCCCATTCGCTTACTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCCGGCAAACCAAGTTGGGAGTTAATCCGAATTCGGCTTCTTCAATGGCAATGGTCttag                                      >  2:3966096/1‑138 (MQ=255)
                                                                                                    tCCTATCGGCGTGTTTTCCTTGCCGATTCGCATCCTGGCTTCCTCAATTGCTGCGTCCTTCATTTCGCCGCCAAAGTCACCCGGGCAAACCATGTTGGCAGTTATTCCGAATTCGGCTTCTTCAATGGCAATGGTCttag                                      >  2:1198148/1‑138 (MQ=255)
                                                                                                          cGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAaag                                <  2:199731/140‑1 (MQ=255)
                                                                                                          cGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAaag                                <  1:422819/140‑1 (MQ=255)
                                                                                                                  tgtCCGTGCCGATTCGCTTCCTGGCTTCCTCTATTGCTGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTACGTCAAAGAAGCCaaa                        <  1:415141/138‑1 (MQ=255)
                                                                                                                         gCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACtt                 >  1:246526/1‑140 (MQ=255)
                                                                                                                              ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa            <  2:4680047/140‑1 (MQ=255)
                                                                                                                                   ttCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGcc       >  2:4119671/1‑140 (MQ=255)
                                                                                                                                   ttCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGACGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCAATTTAGCAGCGcc       >  2:564608/1‑140 (MQ=255)
                                                                                                                                        ggCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGCCAAATg  <  1:4177106/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGCCAAATG  >  NZ_CP034943/2812651‑2812926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: