Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,901,671 A→C 14.6% K411T (AAA→ACA)  EO946_RS14945 → extracellular solute‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,901,6710AC14.6% 477.8 / 26.8 196K411T (AAA→ACA) EO946_RS14945extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (147/13);  new base C (2/34);  total (149/47)
Fisher's exact test for biased strand distribution p-value = 5.38e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

TCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGTT  >  NZ_CP034943/2901536‑2901809
                                                                                                                                        |                                                                                                                                          
tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGTGAATACAAACGa                                                                                                                                        >  2:4868555/1‑140 (MQ=255)
tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                        >  2:4141293/1‑140 (MQ=255)
tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                        >  2:3704532/1‑140 (MQ=255)
  aaaGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACt                                                                                                                                      >  1:4740287/1‑140 (MQ=255)
  aaaGTCGAGCCTTACTTCGACCGTTTATACGTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCGTATGGCTGACATTTTGAGGCCTCAAGAGATTTCGAAGACGCTGGGTAGAGACTACAAACGACt                                                                                                                                      >  2:2819339/1‑140 (MQ=255)
        gAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGa                                                                                                                                >  1:31630/1‑140 (MQ=255)
        gAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGa                                                                                                                                >  1:657637/1‑140 (MQ=255)
        gAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGa                                                                                                                                >  1:4086026/1‑140 (MQ=255)
        gAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGGGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGa                                                                                                                                >  1:3355601/1‑140 (MQ=255)
         aGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAg                                                                                                                               >  1:3703607/1‑140 (MQ=255)
         aGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGGGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAg                                                                                                                               >  2:747831/1‑140 (MQ=255)
          gCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGc                                                                                                                              >  2:4026204/1‑140 (MQ=255)
          gCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGc                                                                                                                              >  2:8143/1‑140 (MQ=255)
                     aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:762869/1‑140 (MQ=255)
                       cttttATATTTGCCAAACTGAATTTTGTTTGTCGTGGGCACGTTCGCCCATTATGTTAGGGCTCAATTTTTGACGCCACAATATTTTTCGAACAAGCGGGTAAGATAATACACACGACTCCGGGAGCAATCGGAAAcaca                                                                                                                 <  2:227514/138‑1 (MQ=255)
                         tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                               >  2:1836211/1‑140 (MQ=255)
                         tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                               >  1:3830140/1‑140 (MQ=255)
                         tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                               >  2:228513/1‑140 (MQ=255)
                         tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                               >  1:2589157/1‑140 (MQ=255)
                          ttATACTTGCCAAACGGACTGTGGTATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACTACTCCGGGAGCAATCTGAAACACAggg                                                                                                              >  2:97437/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:954988/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:339785/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  2:1343913/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:2863339/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:2790086/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  2:2661144/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:143080/1‑140 (MQ=255)
                                  gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  2:550398/1‑140 (MQ=255)
                                   ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  2:1961663/1‑140 (MQ=255)
                                   ccAAAAGGAATGTGGGCTGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGTAGAGAATACAAACGACTCAGGGAGCAATCGGAAACACCGGGAGCTgaaga                                                                                                     >  1:1235221/1‑140 (MQ=255)
                                                     gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:113421/1‑140 (MQ=255)
                                                     gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  1:4400580/1‑140 (MQ=255)
                                                       ccTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAag                                                                                 >  2:1022192/1‑140 (MQ=255)
                                                         tGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGATGGGAAGAGACTACAAACGACTCCGTGAGCAATCGGAAAAACAGGGAGCTGAAGAACTATGAGTGACATCGGAagag                                                                               >  2:1145912/1‑140 (MQ=255)
                                                           ggCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGACACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGAc                                                                             >  1:3068229/1‑140 (MQ=255)
                                                           ggCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGAc                                                                             >  1:876398/1‑140 (MQ=255)
                                                           ggCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGAc                                                                             >  1:523839/1‑140 (MQ=255)
                                                           ggCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGAc                                                                             >  1:2342525/1‑140 (MQ=255)
                                                             cACGGTCGCACCGGAGGTTTGGGCTGAAATTTTGACGCCACACGAGATTTCGAAGAAGGTGGTAAGACAAAAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGt                                                                           <  2:3269599/140‑1 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGACGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:2072767/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGACACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:2855985/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:634861/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:3975651/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:3860956/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:2765052/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:2428836/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:2828068/1‑140 (MQ=255)
                                                                ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAGGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:1791866/1‑140 (MQ=255)
                                                                 gTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAt                                                                       >  2:4688776/1‑140 (MQ=255)
                                                                 gTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAt                                                                       >  1:2789684/1‑140 (MQ=255)
                                                                  tCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAtg                                                                      >  1:1866364/1‑140 (MQ=255)
                                                                  ggggACTGGCGTTTTTGGCTCACTTTTTCACGCGTCAAGTGTTTTCCAAGAAGGGGGCACCTCAATACACACGACTCCGGGAGCAATCGGAAACACCGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAtg                                                                      <  2:2397646/138‑1 (MQ=255)
                                                                       atggtgCTTTTGGCTGAATTTTTGCCGCATAAAGTTTTTTCTAATAAGCTGGTATTAGAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGaaa                                                                 <  2:3499388/135‑1 (MQ=255)
                                                                            gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACCTGGAGCTGACGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGt                                                            >  1:4967050/1‑140 (MQ=255)
                                                                            gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGt                                                            >  1:2922916/1‑140 (MQ=255)
                                                                                aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                        >  2:557523/1‑140 (MQ=255)
                                                                                aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                        >  2:492178/1‑140 (MQ=255)
                                                                                aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                        >  2:1504576/1‑140 (MQ=255)
                                                                                    cttaCATTTTGACGCCTCAAGAGTTTTCGAAGACGGTGGGACGATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                    <  2:4227646/137‑1 (MQ=255)
                                                                                    cTGACATTTTGACGCCTCAAGAGATTTCGAAGACGCTGGGACGAGAATACACACGACTCCGGGAGCACTCGGAAGCACAGGGAGCTGAAGAACAATGAGGGTGATCGCAAGAGACGTTCATGTGACACAGGTGAAGCCGa                                                    >  1:4234486/1‑140 (MQ=255)
                                                                                    cTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                    >  2:3623525/1‑140 (MQ=255)
                                                                                       aCATTTTGACGCCTCAATAGATTTCGAATAAGCTGGGAAGAGAATACAAACGACTCCGTGAGCAAGCGGAAACACACGGAGCTGAAGAACAATGAGTGAGATAGCAAGAGACGTTCATGTGAAACAGGAGAAGCCGaaaa                                                 >  2:1923457/1‑140 (MQ=255)
                                                                                       aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                                 >  1:3750171/1‑140 (MQ=255)
                                                                                       aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                                 >  1:242435/1‑140 (MQ=255)
                                                                                         tttttGACGCCTAAATAGTTTCCCAACAAGCGGGGACCACAACACCCACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAg                                               <  1:3587637/139‑1 (MQ=255)
                                                                                           tttGACCCCTAACGAGATTTCTAAGATGCTGGGAATTTAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCa                                             <  1:4927575/140‑1 (MQ=255)
                                                                                              gccGCCTCATGATATTTCGACGACGCTGGGAATACAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTc                                          <  2:1772884/138‑1 (MQ=255)
                                                                                              gacgacACAAGATATTTCGATGAAGGTGTGATAATAAAACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTc                                          <  2:2549370/140‑1 (MQ=255)
                                                                                              gACCCCTCAACCTATTTCGAACAAGGGGGGAACAGAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTc                                          <  2:3955939/140‑1 (MQ=255)
                                                                                                  ccTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCa                                      >  2:2376960/1‑140 (MQ=255)
                                                                                                  ccTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCa                                      >  2:881257/1‑140 (MQ=255)
                                                                                                   cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                     >  1:1523732/1‑140 (MQ=255)
                                                                                                   cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                     >  1:3165777/1‑140 (MQ=255)
                                                                                                   cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                     >  1:4653715/1‑140 (MQ=255)
                                                                                                   cTCAAGAGATTTCGAAGAAGATGTTAAGAGAATACAAACTAATCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                     >  2:3367890/1‑140 (MQ=255)
                                                                                                    taaaGAGATTTCGAAGATGCTGGTAACATAATACCCACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACt                                    <  1:4820650/138‑1 (MQ=255)
                                                                                                          tttttCGATTATGCTGGTAAGATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtg                              <  2:4780785/138‑1 (MQ=255)
                                                                                                           ttttCCAAGACGGGGGCAAGACAACACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  1:275264/139‑1 (MQ=255)
                                                                                                           ttttCCAAGACGCTGGTAACACAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  2:1364611/139‑1 (MQ=255)
                                                                                                           acttCGAAGACGCTGGCACCACAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAACCCGAAAAAGCAGTCCTCACTTtggtgg                             <  2:3191735/138‑1 (MQ=255)
                                                                                                           aTTTCGAATATGCTGGTAACAAATACAAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  1:4860972/140‑1 (MQ=255)
                                                                                                             ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  1:2620602/1‑140 (MQ=255)
                                                                                                             ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  1:2215819/1‑140 (MQ=255)
                                                                                                             ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  1:1008078/1‑140 (MQ=255)
                                                                                                             ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  1:4831890/1‑140 (MQ=255)
                                                                                                             ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  1:1350743/1‑140 (MQ=255)
                                                                                                             ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  1:350266/1‑140 (MQ=255)
                                                                                                             ttCCAACAAGCCGGTAACAGAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           <  2:4086026/140‑1 (MQ=255)
                                                                                                              tccaagaagCTGGTAAGAGAAAACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATg                          <  2:1110886/137‑1 (MQ=255)
                                                                                                              tccaagaacCTGGGAAGATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATg                          <  2:2873201/137‑1 (MQ=255)
                                                                                                              tCGAACAAGGTGGTAACACAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATg                          <  2:4657417/140‑1 (MQ=255)
                                                                                                                gaacaaGCTGGGAAGACAATAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGta                        <  2:3855445/140‑1 (MQ=255)
                                                                                                                  agaagCGGGCAATATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGtata                      <  2:1450078/140‑1 (MQ=255)
                                                                                                                   catgCTGGGACGACAACCCACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACACTGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTatat                     <  2:3127859/137‑1 (MQ=255)
                                                                                                                               agaATACAAACGAATCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTCAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAATGCAGTCCTCACTTTTGTGGATGTATATTCCTGCATTGct         >  2:2924771/1‑140 (MQ=255)
                                                                                                                                 aacacacacGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:97437/137‑1 (MQ=255)
                                                                                                                                 aacacacacGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:4191694/137‑1 (MQ=255)
                                                                                                                                 aaTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:3659546/140‑1 (MQ=255)
                                                                                                                                    aCAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCg    >  2:4667438/1‑140 (MQ=255)
                                                                                                                                       caaCGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGACGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGtt  <  1:1155341/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGTT  >  NZ_CP034943/2901536‑2901809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: