Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 177,101 G→T 6.5% M1F (TTG→TTT) † EO946_RS01025 → SulP family inorganic anion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,1010GT 6.5% 728.0 / 13.6 276M1F (TTG→TTT) †EO946_RS01025SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base G (72/178);  new base T (24/2);  total (96/180)
Fisher's exact test for biased strand distribution p-value = 2.03e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

CCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATGGTCGGTC  >  NZ_CP034943/176962‑177240
                                                                                                                                           |                                                                                                                                            
ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTGTACCGTAAAATAAAGAAACCTCCCAAAAAAACAAAAAGGGTGCGGATTTAAGTTTTAAAAAGGAGGCGTGATAATTg                                                                                                                                              >  2:4582338/1‑140 (MQ=255)
ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTGAATTATCCCCTAAAATAAACAAAGCACCCAATACAAAAAAAACGGTGCGGGTTTATTTTTTAAAAAGTCGTAGTTATATTTg                                                                                                                                              >  2:4478991/1‑140 (MQ=255)
   tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCCTAAAATAAAAAAAGCGCCCAAAAAAAAAAATACGGTGCGGGTTTTTTTTTTATAAAGGAGTGGTTATATTTGaat                                                                                                                                           >  2:1860166/1‑140 (MQ=255)
           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                                                                  <  1:2017786/140‑1 (MQ=255)
           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                                                                  <  1:1806078/140‑1 (MQ=255)
           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                                                                  >  2:2891340/1‑140 (MQ=255)
           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                                                                  <  1:260981/140‑1 (MQ=255)
                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAATACGGTGCGGATTTATTTTTTTAAAATGAGTGGGTATATTTGAATTATAGTTTTAGAt                                                                                                                             >  2:4706896/1‑140 (MQ=255)
                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAAAAACGTGGGGTTTTATTTTTTAAAAACTAGTGGGTATAATTTTATAAAAGtttttaat                                                                                                                             >  2:4446918/1‑136 (MQ=255)
                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTTCGTTGATTTGTGCCGTAAAATAAATAAAACTCCCCAAACAAAAAAAAAGGGGGGGGTTTATTTTTTTAAAATGAGTGGGTTTTTTTTTATAATATGttttgaa                                                                                                                              >  1:2331770/1‑137 (MQ=255)
                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCAATTCTCCCGTAAAATAAAGAAAGCTCCCAAAAAAACAAATACGGTGCGGAGTTATTTTTTAAAAAGGACTCGTTAGATTTTAATAAAAGTTTTaaaa                                                                                                                             >  2:3332175/1‑137 (MQ=255)
                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGGGGGGCTTTATTGTTTAAAAAGGAGTCGTTATATTTTAAAAATTGTTTTACATTaa                                                                                                                          >  2:1088792/1‑140 (MQ=255)
                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGAATTGTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATAAGGTGGGGATTTATTTTTTAAAAAGGAGGGGTTATATTTTAATAATATTTTTAGATTaa                                                                                                                          >  2:4263892/1‑140 (MQ=255)
                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTaa                                                                                                                          <  2:1766697/140‑1 (MQ=255)
                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCGGTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAACAAAAACGGGGCGGGTTTATTTTTTATAAAGGAGTCGGTATAATTTAATAAAATTTTTACATTaa                                                                                                                          >  2:4836285/1‑140 (MQ=255)
                        aCGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGGAAAAAAAAAAAAGCAGCCAAAACAAAAAATAAGGGGCGGGTTTATTTTTTAAAAAGGAGTCGGTGGATTTTTGTAATATTTTTTAATTAAagcag                                                                                                                     >  1:968266/1‑140 (MQ=255)
                         cGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAATAAAGCTCCCAAAACAACAAATACGGTGGGGGTTTATTTTTTAAAAAGGAGTCGTTATATTTTTATTATAATTTTTAATTAAaggagc                                                                                                                    >  2:4152383/1‑140 (MQ=255)
                          gtgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAgcagca                                                                                                                   <  1:4538630/140‑1 (MQ=255)
                          gtgtCCTCTGTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGCTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTGTCCATTAAAgcagca                                                                                                                   <  2:2024902/140‑1 (MQ=255)
                           tgtCCTCTTTTTCTGCGGTCGTTTTCCTTTCATTTCTCCATTAAAATATAGAAATCTCCCAATAAAACAAAGACTGTGCTGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                                                  <  2:4655913/140‑1 (MQ=255)
                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAGAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                                                  <  2:3465984/140‑1 (MQ=255)
                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                                                  <  2:1239150/140‑1 (MQ=255)
                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                                                  <  2:2467988/140‑1 (MQ=255)
                            gtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAAt                                                                                                                 <  1:4104564/140‑1 (MQ=255)
                             tCCTCTTTTTCTCCGGTCGTTTTACTTTGAATTCTACCCAAAAATAAAATAAGCTCCCAAAAAAACAAATTCGGTGCGGGATTATTTTTTAAAAAGTAGTCGTTATATGTGTATAAAAGTTTTTAAATAAAGAACCaaag                                                                                                                >  2:806196/1‑138 (MQ=255)
                             tCCTCTTTTTCTCCGGTCGTTTTACGTTCATTTGTCCCGTAAAAAAAAAAAAGCTCCCCATACAAAAAAAAAGGGGCGGATTTATTTTTTAAAAAGGAGGGGGTATATTTGAATAATAGTTTTAGATTTAaacagccagt                                                                                                                >  2:4109329/1‑136 (MQ=255)
                                      tctcCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATCCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGaa                                                                                                       <  2:2847430/140‑1 (MQ=255)
                                         cctgTCGTTTTCCTTCGATTTCTCCCGTAAAATACAGAAATCTCGCACTACAACAAATACGGTTTGGATTTATTTTTTAACAAGGATTCGTTATATTTTAATAATAGTTTTACATTAAAGCAGGAATGGTTCGGGAatat                                                                                                    <  2:1454456/137‑1 (MQ=255)
                                         ccGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAatat                                                                                                    <  2:3388719/140‑1 (MQ=255)
                                                               tcCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGATGGc                                                                              >  1:3562819/1‑140 (MQ=255)
                                                                      aaaTAAAGAAAGCTCCCAATACAACAAATACGGTGCTGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                                                       <  1:1458064/140‑1 (MQ=255)
                                                                      aaaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGTATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                                                       <  1:1249257/140‑1 (MQ=255)
                                                                      aaaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                                                       <  1:1916953/140‑1 (MQ=255)
                                                                            agaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt                                                                 <  1:379757/140‑1 (MQ=255)
                                                                            agaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt                                                                 <  1:2366396/140‑1 (MQ=255)
                                                                             gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                                <  2:833145/140‑1 (MQ=255)
                                                                             gaaacCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                                <  2:239035/140‑1 (MQ=255)
                                                                             caaagCTCCCAATACAACACATCCGGTGCTGATTTAATTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTGACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                                <  2:3151512/139‑1 (MQ=255)
                                                                              aaagCTCCCAATACCACAAATACGGTGCGGATTTAATTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTa                                                               <  1:1083172/140‑1 (MQ=255)
                                                                                  cTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAt                                                           >  2:372027/1‑140 (MQ=255)
                                                                                    cccAATACAACAACTCCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                                                         <  2:2583821/140‑1 (MQ=255)
                                                                                    cccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                                                         <  2:4190144/140‑1 (MQ=255)
                                                                                    cccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                                                         <  2:1211348/140‑1 (MQ=255)
                                                                                            aacaaATACGGTGCGGATTTATTTTTTAAAAGGGAGTCGGTATATTTGAATAATAGTTTTACATTAAATCATCAAGTGTTCGGGAATATCCCAAATGACATTCTTGCTGGCCATTTAGTTGCGTTAGCATTCATACCGGa                                                 >  2:1464799/1‑140 (MQ=255)
                                                                                            aacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAATCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGa                                                 >  1:4693304/1‑140 (MQ=255)
                                                                                            aacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTTCGTTAGCATTAATACCTGa                                                 >  1:2789925/1‑140 (MQ=255)
                                                                                             ccaacgaCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGac                                                <  1:2243288/134‑2 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGGTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa                                            >  2:4709876/1‑140 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa                                            >  2:1124923/1‑140 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa                                            >  2:707991/1‑140 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa                                            >  1:1055490/1‑140 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTAAATTAAAGCAGCACTGGTTCGGGAATATCCGAAAAGACAGTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCac                                            >  2:2927743/1‑139 (MQ=255)
                                                                                                   aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTTCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt                                          >  1:2517938/1‑140 (MQ=255)
                                                                                                   aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGa                                                 <  1:4567057/133‑1 (MQ=255)
                                                                                                   aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAAtt                                          >  2:273524/1‑140 (MQ=255)
                                                                                                   aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATAACTGa                                                 >  2:501262/1‑133 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTATTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                       >  1:741055/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                                <  1:2143884/115‑1 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                       >  2:1583834/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                       >  1:3410311/1‑140 (MQ=255)
                                                                                                        gCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAATACATTCTTTCTGGCATTTTATTTGCGTTAGCATTAATAACTGAAGCAATTTGCtt                                     >  1:4122951/1‑140 (MQ=255)
                                                                                                         cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTACAGCAGCCATGGTTCGGGACTATCCGAAATGTCATGCGTGCGGGCATTGTAGGTGGGGTTGCATTCATACCTGACGCAATTGGCttg                                    >  1:4260999/1‑139 (MQ=255)
                                                                                                         cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt                                    >  1:3696515/1‑140 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                   <  2:968266/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                   <  2:4872025/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                   <  1:207865/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                   <  2:1337852/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                   <  2:3962410/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                   <  2:2080135/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATGACAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                   <  1:3321312/140‑1 (MQ=255)
                                                                                                               tatttTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCtatt                              <  2:2331770/140‑1 (MQ=255)
                                                                                                               tatttTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCtatt                              <  2:2144073/140‑1 (MQ=255)
                                                                                                                 ttttttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAtt                                                          <  1:2745315/110‑1 (MQ=255)
                                                                                                                 ttttttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg                                                  <  1:4206444/118‑1 (MQ=255)
                                                                                                                    tttAAACAGGAGTCGTTATATTTGAATAATAGTTGTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                         <  1:4832792/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                         <  1:610894/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                         <  1:49832/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATACTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                         <  1:3007055/140‑1 (MQ=255)
                                                                                                                    tctAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                         <  1:1704578/138‑1 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCg                       <  2:3657414/140‑1 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCg                       >  2:3893509/1‑140 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCg                       <  2:3529371/140‑1 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCg                       <  2:2425722/140‑1 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCg                       <  2:2550926/140‑1 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCg                       <  2:3013171/140‑1 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTAAATTAAATCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCg                       >  1:3502/1‑140 (MQ=255)
                                                                                                                                      tatTTGAATAATATTTGTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  2:1906118/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAATCAGCAATGGTTCGGGAATATCGGTAATGAGATTCTTGCTGGCTTTTTATTTGCGTTAGCATTTATACCTGAAGGAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  1:806196/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGGAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  2:3611191/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGGATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  2:2694750/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  2:580813/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  2:3233368/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  2:2801727/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  1:3946616/140‑1 (MQ=255)
                                                                                                                                      tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt       <  1:3655283/140‑1 (MQ=255)
                                                                                                                                        tttGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGACGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggtcg     <  2:2252166/140‑1 (MQ=255)
                                                                                                                                        tttGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggtcg     <  2:1025981/140‑1 (MQ=255)
                                                                                                                                        tttGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggtcg     <  1:3826793/140‑1 (MQ=255)
                                                                                                                                          tGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggtcggt   >  1:2385097/1‑140 (MQ=255)
                                                                                                                                          tGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggtaggt   >  2:2118337/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATGGTCGGTC  >  NZ_CP034943/176962‑177240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: