Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,217,708 T→C 6.8% K159K (AAA→AAG sigO ← RNA polymerase sigma factor SigO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,217,7080TC 6.8% 724.7 / 29.7 225K159K (AAA→AAGsigORNA polymerase sigma factor SigO
Reads supporting (aligned to +/- strand):  ref base T (59/149);  new base C (7/8);  total (66/159)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 229 total aligned reads displayed.

ATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGTAGA  >  NZ_CP034943/3217569‑3217844
                                                                                                                                               |                                                                                                                                        
atcatcGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCCAACTGGCCGTTCACAGCGTAGTTTGGCCCTTGTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCGAGCCCt                                                                                                                                          >  1:2982538/1‑140 (MQ=255)
      ggTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTa                                                                                                                                    <  2:1087390/140‑1 (MQ=255)
        tGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATg                                                                                                                                  <  1:4634669/140‑1 (MQ=255)
             aGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGt                                                                                                                             <  2:4348374/140‑1 (MQ=255)
               cATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTCTGAATGTTg                                                                                                                           >  2:127593/1‑140 (MQ=255)
                  tCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGgag                                                                                                                        <  1:2940256/140‑1 (MQ=255)
                  tCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGgag                                                                                                                        <  1:412307/140‑1 (MQ=255)
                      tCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCACACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                                    <  2:1754692/140‑1 (MQ=255)
                      tCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                                    <  2:2152199/140‑1 (MQ=255)
                           tGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGc                                                                                                               <  1:1074809/140‑1 (MQ=255)
                           tGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGc                                                                                                               <  1:611069/140‑1 (MQ=255)
                           tGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTGTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGc                                                                                                               <  2:3703210/140‑1 (MQ=255)
                             ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                                 >  2:208527/2‑140 (MQ=255)
                             ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                                 >  2:2557222/2‑140 (MQ=255)
                             ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                                 >  2:1630596/2‑140 (MQ=255)
                             ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                                 >  2:2626778/2‑140 (MQ=255)
                             ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                                 >  2:3734206/2‑140 (MQ=255)
                             ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTGACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                                 >  2:1253867/2‑140 (MQ=255)
                              tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                            <  2:1213224/140‑1 (MQ=255)
                              tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                            <  1:4852965/140‑1 (MQ=255)
                              tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCACAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                            <  1:381033/140‑1 (MQ=255)
                               cAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCggg                                                                                                           >  1:1786793/1‑140 (MQ=255)
                                cacgTCAACTTTATCGTCGTTTTGGCTC‑‑‑TTGTGTTCAAACGGGCCGTTCACAGCGTACTTTCCCCCCTGTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:2898497/137‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCACACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:2849047/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCCAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  1:1208386/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:2461051/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  1:1604727/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  1:165536/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  1:482037/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  1:1724359/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:757244/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:3268314/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  1:3732280/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  1:3250789/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTATTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:1127482/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTGTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:3864225/140‑1 (MQ=255)
                                aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTGTCCCCCTTTGTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:4499168/140‑1 (MQ=255)
                                       aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                                                   >  2:783515/1‑140 (MQ=255)
                                       aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                                                   >  2:4168473/1‑140 (MQ=255)
                                       aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTATTTATGAATGTTGTAGATGTTTTGACGGGACTCTCCt                                                                                                   >  2:2497522/1‑140 (MQ=255)
                                       aaTTTATCGACTTTTTTGTTC‑‑‑TATTGTTAAAACTGGCCTTTCACATCTTACGATACCCCTTTTTTTTCTCCGCTAGCTGTTTTCTGATATAATCAAGACCTTTTTTATGAATATTGGAGATCTTTAGCAGCTACTCACCt                                                                                                   >  2:4957565/1‑140 (MQ=255)
                                       aaTTGAGCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                                                   <  1:4183483/140‑1 (MQ=255)
                                            aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                                              <  1:3738380/140‑1 (MQ=255)
                                            aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCATTTATATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                                              <  2:634116/140‑1 (MQ=255)
                                                  tttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGTATATGTTTTGCCGGGACTCTCCTAGTGAATCGGc                                                                                        >  1:3203091/1‑140 (MQ=255)
                                                   ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                       <  2:174251/140‑1 (MQ=255)
                                                   ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                       <  2:1609239/140‑1 (MQ=255)
                                                   ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                       <  1:1373374/140‑1 (MQ=255)
                                                          tC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCtt                                                                                >  2:4715563/1‑140 (MQ=255)
                                                          tC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCGTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCtt                                                                                >  1:3854229/1‑140 (MQ=255)
                                                           c‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                                               <  1:64623/140‑1 (MQ=255)
                                                           c‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                                               <  1:1121471/140‑1 (MQ=255)
                                                                   gTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCg                                                                          <  2:1544146/140‑1 (MQ=255)
                                                                           tGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCa                                                                  <  1:546255/140‑1 (MQ=255)
                                                                           tGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCa                                                                  <  1:266574/140‑1 (MQ=255)
                                                                           tGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGGTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCa                                                                  <  1:4538264/140‑1 (MQ=255)
                                                                             gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                          <  2:837806/98‑1 (MQ=255)
                                                                             gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                                <  2:1381512/140‑1 (MQ=255)
                                                                             gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                                <  2:1976153/140‑1 (MQ=255)
                                                                                  tCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGAt                                                           >  2:1764587/1‑140 (MQ=255)
                                                                                       gCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACt                                                      >  2:4433924/1‑140 (MQ=255)
                                                                                                 cccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGACTCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGc                                             >  1:1863838/1‑140 (MQ=255)
                                                                                                  ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                            <  2:3854229/140‑1 (MQ=255)
                                                                                                  ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                            <  2:4846633/140‑1 (MQ=255)
                                                                                                  ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                            <  1:3922512/140‑1 (MQ=255)
                                                                                                  ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                            <  1:2248877/140‑1 (MQ=255)
                                                                                                  ccccTTTTTGTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGTAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                            <  1:4021253/140‑1 (MQ=255)
                                                                                                   cccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCAc                                           >  1:4431074/1‑140 (MQ=255)
                                                                                                    ccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGGCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACg                                          <  1:975840/140‑1 (MQ=255)
                                                                                                      tttttttGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCt                                        >  1:835140/1‑140 (MQ=255)
                                                                                                            tGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                       <  2:2717133/87‑1 (MQ=255)
                                                                                                                      acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc                        <  1:2557222/139‑1 (MQ=255)
                                                                                                                      acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc                        <  1:208527/139‑1 (MQ=255)
                                                                                                                      acTGCTTTCTGATAGTCTCCAGCCCCGTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTGGTTTgtcagtc                        <  1:1014108/139‑1 (MQ=255)
                                                                                                                        tGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGtcagtcag                      <  2:3037058/140‑1 (MQ=255)
                                                                                                                             tCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCAt                                                                 <  2:510187/92‑1 (MQ=255)
                                                                                                                              cTGATATTCTCCAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTg                <  2:2982538/140‑1 (MQ=255)
                                                                                                                                gATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGaa              <  1:2355560/140‑1 (MQ=255)
                                                                                                                                   atTCTCCAGCCCTTTTTTATGAACGCTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGACAGTCAGCTTCTGAATCg           <  1:849281/140‑1 (MQ=255)
                                                                                                                                   atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCTATATCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg           <  1:2497522/140‑1 (MQ=255)
                                                                                                                                   atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg           <  1:1764587/140‑1 (MQ=255)
                                                                                                                                   atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg           <  1:1578509/140‑1 (MQ=255)
                                                                                                                                   atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg           <  2:1088978/140‑1 (MQ=255)
                                                                                                                                   atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg           <  1:4248096/140‑1 (MQ=255)
                                                                                                                                   atTCTCAAGACCTTTTTTATGTATGTTTGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGGACTCTTTTTTGTTTTTCAGTCAGCTTCTGAATCg           <  1:4957565/140‑1 (MQ=255)
                                                                                                                                   atTCACAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg           <  2:401044/140‑1 (MQ=255)
                                                                                                                                    ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                                >  2:3603323/1‑86 (MQ=255)
                                                                                                                                    ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                                <  1:509434/86‑1 (MQ=255)
                                                                                                                                    ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                                <  1:3603323/86‑1 (MQ=255)
                                                                                                                                    ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                                >  2:509434/1‑86 (MQ=255)
                                                                                                                                    ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGc          >  1:2283682/1‑140 (MQ=255)
                                                                                                                                    ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGc          >  1:2757421/1‑140 (MQ=255)
                                                                                                                                     tctcAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCt         <  1:2107930/140‑1 (MQ=255)
                                                                                                                                         aaGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGt     >  2:1191169/1‑140 (MQ=255)
                                                                                                                                         aaGCCCTTTTTTATGAAAGTTGGAGATGATTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCAGGAAGAAAGACTTTCGTGAGCACGCGGTTGTGAGGGTCAGTCCGCTTCTGAATCGCGTggg     >  2:3177828/1‑139 (MQ=255)
                                                                                                                                          aGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGTa    <  2:4755500/140‑1 (MQ=255)
                                                                                                                                          aGCCCTTTTTGATGCATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCGTGCATCGTGGCACCATGACGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGTa    <  1:2032846/140‑1 (MQ=255)
                                                                                                                                            cccTTTTTTATGAATGTTGTAGATGTTTTGCCTGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCAACATGAAGATAGACTTTCTTCAGCAAGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGTaga  <  1:1933939/140‑1 (MQ=255)
                                                                                                                                            cccTTTTTTATGAATGTTGGAGATGATGTGCCTGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGTaga  <  1:3509286/140‑1 (MQ=255)
                                                                                                                                            cacTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGTaga  <  1:609577/138‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                        
ATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGTAGA  >  NZ_CP034943/3217569‑3217844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: