BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,219,740	C→A	7.9%	H360N (CAT→AAT)	oxdC →	oxalate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,219,740	0	C	A	7.9%	684.5 / 10.6	265	H360N (CAT→AAT)	oxdC	oxalate decarboxylase
Reads supporting (aligned to +/- strand): ref base C (154/78); new base A (3/27); total (158/107)
Fisher's exact test for biased strand distribution p-value = 2.47e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

TCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACC  >  NZ_CP034943/3219601‑3219879

tCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCCATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGc                                                                                                                                             >  1:766753/1‑140 (MQ=255)

    tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAACTCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACCTTCGTTCAACGGCAGCt                                                                                                                                         >  2:2892745/1‑140 (MQ=255)

    tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAATACGATCATTATGACGATGTATCTTTAAACCAATGGCTGGCAATGCTTCCTGAAACATTAGTTAAACAGCATCt                                                                                                                                         >  1:2779086/1‑140 (MQ=255)

    tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                         >  1:3448505/1‑140 (MQ=255)

    tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                         >  1:1575465/1‑140 (MQ=255)

    tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                         >  1:2473320/1‑140 (MQ=255)

             ggTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAgg                                                                                                                                >  2:2117066/1‑140 (MQ=255)

              gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                               >  2:1273696/1‑140 (MQ=255)

              gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                               <  1:465956/140‑1 (MQ=255)

              gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                               >  2:4978560/1‑140 (MQ=255)

              gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTAGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                               >  1:4857892/1‑140 (MQ=255)

                cATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCTATGTATCTTTAAACCAATGGCTTTCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGaaa                                                                                                                             <  2:3416702/140‑1 (MQ=255)

                cATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGaaa                                                                                                                             <  2:2637583/140‑1 (MQ=255)

                aaTTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGaaa                                                                                                                             <  2:3063602/139‑1 (MQ=255)

                 aTTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCGTGAAACATTAGTTCAACAGCATGTTGATTTAGGaaaa                                                                                                                            >  2:2722508/1‑140 (MQ=255)

                 aTTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGaaaa                                                                                                                            >  2:4803558/1‑140 (MQ=255)

                 aTTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGaaaa                                                                                                                            >  2:3311305/1‑140 (MQ=255)

                 aTTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATAAGTTCAACAGCATCTTGATTTAGGaaaa                                                                                                                            >  2:4184002/1‑140 (MQ=255)

                  ttACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAAAGCTTCCTGAAACATTAGTTCAACAGCATCTTGATGTAGGAAAAg                                                                                                                           >  1:4937093/1‑140 (MQ=255)

                  ttACGTTGAAAACATCGGGGACGAACCGCTTGTCTTGTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAATCCAATGGCTGGCCATGCTTCCTGACGCCTTAGTTCAACAGCATCGGGATTTAgggccag                                                                                                                           >  1:3794318/1‑135 (MQ=255)

                   tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                          >  1:4731707/1‑140 (MQ=255)

                   tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                          <  1:1033134/140‑1 (MQ=255)

                   tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                          <  1:911459/140‑1 (MQ=255)

                   tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                          <  2:1822394/140‑1 (MQ=255)

                       ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACCTTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                      >  2:2295009/1‑140 (MQ=255)

                       ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                      >  2:3932874/1‑140 (MQ=255)

                        tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTCGGAAAAGACTTTa                                                                                                                     >  2:395967/1‑140 (MQ=255)

                        tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTa                                                                                                                     >  2:4850806/1‑140 (MQ=255)

                        tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTa                                                                                                                     >  1:2979327/1‑140 (MQ=255)

                        tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCGTGATTTAGGACAAGACTTTa                                                                                                                     >  2:4055814/1‑140 (MQ=255)

                        tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGAAGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTa                                                                                                                     >  1:1055248/1‑140 (MQ=255)

                               aTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGatat                                                                                                              >  1:695421/1‑140 (MQ=255)

                                 cGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGACAAGACTTTACTGATATTc                                                                                                            >  1:2207430/1‑140 (MQ=255)

                                 cGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTAATGATAtta                                                                                                            >  1:3741632/1‑139 (MQ=255)

                                            cGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCgaaagaa                                                                                                 <  2:2432399/140‑1 (MQ=255)

                                            cGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCgaaagaa                                                                                                 <  1:3976622/140‑1 (MQ=255)

                                              cTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCCATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGaaaa                                                                                               >  1:3808297/1‑140 (MQ=255)

                                                 gTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCa                                                                                            <  2:1556249/140‑1 (MQ=255)

                                                    tttttAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATcc                                                                                         <  2:703382/140‑1 (MQ=255)

                                                            aaTCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAAGGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTaa                                                                                 <  1:1967789/140‑1 (MQ=255)

                                                                tttAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGAATCTTGATTTAGGAAAAGACTTTAATGATATTCTTTCGAAATAAAAGAATCCGGTTGTaaaaaa                                                                             >  1:2688337/1‑140 (MQ=255)

                                                                   aaaGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGaa                                                                          <  2:2134222/140‑1 (MQ=255)

                                                                   aaaGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGaa                                                                          <  2:2842341/140‑1 (MQ=255)

                                                                   aaaGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGaa                                                                          <  2:909429/140‑1 (MQ=255)

                                                                         gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAg                                                                    >  1:1791538/1‑140 (MQ=255)

                                                                         gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAg                                                                    >  1:3163359/1‑140 (MQ=255)

                                                                         gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCAGCCGGTTGTAAAAAAGAAATGCAg                                                                    >  2:2586638/1‑140 (MQ=255)

                                                                          aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCACTGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTAGTGATCTTCTTTCGAAAGAAAAGCAGCCGGTTGTAAAAAAGAAATGCAGt                                                                   >  2:3761426/1‑140 (MQ=255)

                                                                          aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                   >  2:800182/1‑140 (MQ=255)

                                                                          aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                   >  1:3359778/1‑140 (MQ=255)

                                                                                   ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGACAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTACATaaaaa                                                          >  1:2111310/1‑140 (MQ=255)

                                                                                      aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                       >  1:837728/1‑140 (MQ=255)

                                                                                      aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                       >  2:617375/1‑140 (MQ=255)

                                                                                      aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                       >  1:4291964/1‑140 (MQ=255)

                                                                                      aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                       >  2:388469/1‑140 (MQ=255)

                                                                                       tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGa                                                      >  2:2414371/1‑140 (MQ=255)

                                                                                       tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGa                                                      >  2:4793156/1‑140 (MQ=255)

                                                                                       tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTGTCGAAAGAAAAGCAAACGGTTGTAAAAAAGAAATGCAGTAACTAAAAAACGa                                                      >  1:2919042/1‑140 (MQ=255)

                                                                                       tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGAATTAGGAAAAGACTTTACTGATATTCTTACCAAATAAAAGCATCAGGATGTAAAAAAGAAATCCAGTAAATAAAAAACGa                                                      >  2:4948267/1‑140 (MQ=255)

                                                                                       gacagcTTTAAACCATGGGTTTGAATTGCTTCCTCAAACATTAGTTCAACAGCATCTTGTTTTAGTAAAAGATTTTAGTGATTTTCTTTCGAAATAAAAGCATCGGGTTTTAAAAAATAAAGCCATTAAATAAAAAACGa                                                      <  2:2872473/135‑1 (MQ=255)

                                                                                            ttttAAACAAAGGGTTTTAATTGATTCCTTAAACATTAGTTCAAAATCATTTTTATTTTGTAAAAGATTTTTCTGATTTTATTTCGACAGAAAATCATCGGTTTTTAAAAAAGAAATGCATTAAATAAAAAACGACCAGc                                                 <  2:2688337/139‑1 (MQ=255)

                                                                                                    cAAGGGCTTCCAATGCTTCCTGAAACATTATTACAACACCATCTTGATTTAGCAAAATACTTTACTGATATACTTTCTAAACAAAAGCATCCGGTTTTAAAAAACAAATCCAGTAAATAAAAAACGACCAGCTTGCagag                                         <  2:2108050/140‑1 (MQ=255)

                                                                                                    acagggtttGCAATGCTTCCTTAAACATTATTTCACCCGAATCTTTTTTTAGGAAAAGACTTTACTGTTATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagag                                         <  2:2979327/133‑1 (MQ=255)

                                                                                                      aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                       >  1:817734/1‑140 (MQ=255)

                                                                                                      aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                       >  1:411258/1‑140 (MQ=255)

                                                                                                       tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGc                                      >  1:294648/1‑140 (MQ=255)

                                                                                                       tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGc                                      >  1:1849077/1‑140 (MQ=255)

                                                                                                         gCTTGAAATGCTTCCTGAAAAATTAGTACAACAGCATCTTTATTTTTTAAAAGACTTTAATGATATTCTTTCCAAACAAAACCATCCGGTTTTAAAAAATAAATGCAGAAAATAAAAAACGACCAGCTTGCAGAGAGCaa                                    <  2:2005439/140‑1 (MQ=255)

                                                                                                         gCTGGCATTGCTTCCTGAAACATTAGTACAACAGCTTCTTGTTTTAGGAAAAGACTTTACTGATTTTCTTTCGAAAGAAAAGCACCCGGTTGTAAAAAATAAATGCAGTAATTAAAACACGACCAGCTTGCAGAGAGCaa                                    <  2:2743793/140‑1 (MQ=255)

                                                                                                            tGCAAAGCTACATTAAACATTAGTACAACAGCATCTTGATTTAGCAAAAGACTTTACTGATATACTTCCGAAACAAAAGCATCCGGTTGAAAAAAAGAAAGGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCg                                 <  2:3779214/140‑1 (MQ=255)

                                                                                                              cattggttCCTGAAACATTCGTTCAACCGCTTCTTGATTTCGGAAAAGACTTTACTGTTATGGTTTCGAAACAAAAGCAGCCGGTTTTAAAAAATAACTGCTGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGtt                               <  2:424906/134‑1 (MQ=255)

                                                                                                              cactggttCCTTAAAAATTAGTCCAACAGATTCTTTATTTAGCAAAAGAATTTACTGATATTCTTTCGAAATAAAACCATCCGGTTGTAAAAAAGAAATCCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                               <  2:1176831/134‑1 (MQ=255)

                                                                                                              cAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCACTCGtt                               >  1:3408418/1‑140 (MQ=255)

                                                                                                              cAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTGTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                               >  1:1527014/1‑140 (MQ=255)

                                                                                                              cAATGCTTCCTGAAAATTTAGTTCAACAGCATCTTGATTTAGGAAAATACTTTACTTATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                               <  2:3516468/140‑1 (MQ=255)

                                                                                                              cAATGCTTCCTCAAAAATTAGTAAAACAGCATCTTTATTTCCCAAAACAATTCACTGATATTCTTACGAAATAAAACAAACCGGTTGAAAAAAAGAAATGCAGAAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                               <  2:573990/140‑1 (MQ=255)

                                                                                                              ataTGCTTCATCAAAATTTAGTTCAACAGAATCTTGTTTTTTCAATCTACTTTACTGTTATTATTTGGAAAGAAATGCTTCCGTTTGTAAAAAACAAATGCAGAAAATAAAACACGACCAGCTTGCAGAGAGCAATCGtt                               <  2:311946/138‑1 (MQ=255)

                                                                                                              aaaTGCTTCCTCAAACATTATTTCAAAAGCTTTTTTATTAAGTAAAAGAATTTACTTATATCATTTCCAAAGAAAAGCAGCGTGTTTAAAAAAAAAAATCAATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                               <  2:715443/139‑1 (MQ=255)

                                                                                                                   cttacttaAACATTAGTTAAAAAGAATCTTTATTTATGAAAATAATTTATTTATATGCTTTCCAAATAAACGAATCCTGTTTTAAAAAATAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                          <  2:3174618/140‑1 (MQ=255)

                                                                                                                   cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                          >  2:4633759/1‑140 (MQ=255)

                                                                                                                   cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                          >  2:2163327/1‑140 (MQ=255)

                                                                                                                   cTTCCTGAAACATTAGTTCAACAGCATCTGGATTTCGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                          >  2:1213537/1‑140 (MQ=255)

                                                                                                                    ttCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGc                         >  2:3666355/1‑140 (MQ=255)

                                                                                                                     tCCTGAAAAATTAGTTCAACACCATCTTGATTTAGTAAAAGAATTTAATTATATTCTTTCTAAAGAAAAGAATCCGGTTGTAAAAAATAAATGAAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                        <  2:3379502/140‑1 (MQ=255)

                                                                                                                      ccTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCaa                       >  2:1480531/1‑140 (MQ=255)

                                                                                                                       cTGAAACATTAGTTCAACAGCATATTTATTTAGTAAAAGACTTTCATGATATTCTTTCGAAAGAAACGCATCCGTTTGTAAAAAAGAAATGCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg                      <  2:1040341/140‑1 (MQ=255)

                                                                                                                        tcaaaCTTTAGTTCACAAGAATGTTTTTTTAGCAAAATTCTTTAGTCATATTCTTTCCAAAAAAAAGCATCCGTTTTTAAAAAAAAACTGCATAAAAAAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGc                     <  1:3209552/138‑1 (MQ=255)

                                                                                                                        tGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGc                     >  2:3089536/1‑140 (MQ=255)

                                                                                                                           aaCATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAATCCtt                  >  1:2636567/1‑140 (MQ=255)

                                                                                                                           aaCATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCtt                  >  2:466830/1‑140 (MQ=255)

                                                                                                                               tttttacAACAGATTTTTTATTTATCAAAATAATTTAATCATATTATTACAAAACAAAACCATCCTTTTTAAAAAAAAAAATCCAAAAAAAAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata              <  1:1480559/134‑1 (MQ=255)

                                                                                                                               ttATTTAAACAGAATTTTTATTTAGTAAAATAATTTACTGATATTCTTTCGAAACAAAAGCATCGGGTTTTAAAAAATAAATGCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata              <  2:1935928/140‑1 (MQ=255)

                                                                                                                               ttAGTCCAACCGAATGTTTATTTCGGAAACAACATTCCTCATATTCTTTCTAACTAAAAGCATCCGGTTTTAAAAAACAAATGCACTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata              <  1:1224221/140‑1 (MQ=255)

                                                                                                                                tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa             >  2:2455384/1‑140 (MQ=255)

                                                                                                                                tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATACTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTATCTGCAAGCCTTTATaa             >  2:4524761/1‑140 (MQ=255)

                                                                                                                                  tttaaaCAGCATTTTTATTTAGTAAAATACTTTACTGATATACTTTCTAAATAAAAGCATCCTGTTTTAAAAAAGAAATGAAGAAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAgg           <  1:3624358/136‑1 (MQ=255)

                                                                                                                                    tCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTGTATAAGgag         >  1:3567489/1‑140 (MQ=255)

                                                                                                                                        cAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTAGAAAGATAAGCATCCGGTGGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTAGCAGAGAGCAATCGGTCGCTGCAAGCCTTTATATGGAGATGt     >  2:2368013/1‑140 (MQ=255)

                                                                                                                                           cATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAGGCCTTTATAAGGAGATGTAcc  >  2:1863631/1‑140 (MQ=255)

                                                                                                                                           cATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTCTAAGGAGATGTAcc  >  2:3030202/1‑140 (MQ=255)

TCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACC  >  NZ_CP034943/3219601‑3219879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`