BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,377	G→T	7.0%	Q115K (CAA→AAA)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,377	0	G	T	7.0%	757.8 / 2.2	303	Q115K (CAA→AAA)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base G (191/70); new base T (2/37); total (193/110)
Fisher's exact test for biased strand distribution p-value = 9.36e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

AGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTT  >  NZ_CP034943/3280239‑3280514

aGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGa                                                                                                                                          >  2:314715/1‑140 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                         >  2:4144466/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:3569941/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:1386806/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:1105983/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  1:786086/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  1:664890/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  1:4564337/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:417051/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  1:3651235/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  1:333588/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  1:1296586/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAGTa                                                                                                                                       >  1:4950973/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTATCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                       >  2:1442288/1‑140 (MQ=255)

      cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                                                    >  2:2861624/1‑140 (MQ=255)

        cGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGa                                                                                                                                  >  2:568660/1‑140 (MQ=255)

         ggTCACCCTTAATATAACAAGCGGAATTTAATTTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGACTGTTGATTAAAAGAt                                                                                                                                 >  2:1828219/1‑140 (MQ=255)

         ggTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAt                                                                                                                                 >  2:3318763/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTGAAAGATa                                                                                                                                >  1:4203814/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAt                                                                                                                                 <  2:3221496/139‑1 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                >  2:4857030/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAAGTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                >  1:3379609/1‑140 (MQ=255)

                    atatAACAAGCTGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGAttttt                                                                                                                      >  1:3611408/1‑140 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                    <  1:1625497/140‑1 (MQ=255)

                        accacgCGGAATATAATGAAAAAGAAAGTCGCTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                                                                  <  2:3485151/135‑1 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                    >  2:491156/1‑137 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:2052797/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:2763114/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:3073239/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:1186789/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:328117/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:4618701/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:2828150/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:3777917/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  1:2774883/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAAATGGTAGAGCAACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAg                                                                                                                 >  2:1315712/1‑140 (MQ=255)

                             tcgtaatttaatttAAAACAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTATAATTAATTGGTAGAGCCACTCAAAATAAACACGAGCATTTTTGTAAAATTAATGTTGATTAAAATATAAGTTATTTTTATCAGaaaa                                                                                                             <  2:255892/136‑1 (MQ=255)

                             gctgAATTTAATTTAAAAGAAAGTCGGTCGGTTTTTTCTTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:3270086/137‑1 (MQ=255)

                             gctcattttaatttaaAACAAAGTGGTTCGTTTTATTCTTTTTCAATTTTTATAATTAATTGTTAGAGCCAATCAAAATCAACCCGAGCCTTTTTTTAAAATTATTGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:3014219/134‑1 (MQ=255)

                             gcgcAATTTAATTTAAAACAAAGTCGGTCGGTTTATTCGTTTTCAAATTTTCTATTTAATTGGTAGAGCCACTCAAAATCAACCCGCGCCTTTTTGTAAAATGATTGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:2822113/140‑1 (MQ=255)

                             gCGTAATTTAATGTAAAAGAATTTCGTTGGGTGTATTCTTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATTAATGTTGATTAAAAGATAAGTTATTTTTATCAGaaaa                                                                                                             <  2:2423184/140‑1 (MQ=255)

                             gCGTAATATAATCTAAAAAAAAGCCGGCCTCTGTATTCTTTTTCAAATTTTATAATTAATAGCTACAGACACACAAAATCAAATCTAGCCTTTTTGAAAATTGAATGTTGATAAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:2887194/140‑1 (MQ=255)

                             gCGGAATTTAATTTAAAAGAACTTCGTTCGTTTTTTTCTTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACACGAGCCTTTTTTTAAAATGAATGTTGATTAAAAGATAAGTTATTTTTATCAGaaaa                                                                                                             <  2:4242411/140‑1 (MQ=255)

                             gCGGAATTTAATTTAAAACAAATTCGGTGGGTTTTTTGTTTTTCAAATTTTATAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCATTTTTTTAAAATTAATGTTGATTAAAATATAAGTGATTTTTATCAGaaaa                                                                                                             <  2:728278/140‑1 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTCAAAGAGAAGTGATTTTTATCAGaaaa                                                                                                             >  1:7564/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  1:2209592/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  1:2176683/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  1:4887853/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                             >  1:1973552/1‑140 (MQ=255)

                             cccgAATTCAATGCAAAACAAAGCCGCTCGGTGTATTCGTTTACCAATTTTCTAATAAATTGGTACAGCCAATAAAAAACAACACTACCCTTTTTGTAAAATGAATGTTGTTTAAAAGATACGTCATTTTTATCAGaaaa                                                                                                             <  2:1719031/137‑1 (MQ=255)

                               ggAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACg                                                                                                           >  2:384260/1‑140 (MQ=255)

                                                  agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGTTAAGTGATTTTTATCAGAAAACGACCCTCTACCGACTgaaga                                                                                        >  2:2468428/1‑140 (MQ=255)

                                                  agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACTACCCTATACAGACTgaaga                                                                                        >  2:4407119/1‑140 (MQ=255)

                                                    tcggtcggTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaa                                                                                      >  2:1216633/1‑140 (MQ=255)

                                                    tcggtcggTGTATTCGTTCTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAAAGACCCTATACCGACTGAAGaaa                                                                                      >  1:557172/1‑140 (MQ=255)

                                                      ggtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGAGCCTATACCGACTGAAGaaaaa                                                                                    >  2:40298/1‑140 (MQ=255)

                                                      ggtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaa                                                                                    >  2:1481428/1‑140 (MQ=255)

                                                              tATTTGTTTACAAATTTTATAATTACTGGGTAGAGCCACTCAAAAACCACACCACCCTTTTGTTAAAATTAATGTTTATTAAAAGATAAGTGATTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                            <  1:866624/140‑1 (MQ=255)

                                                                  ttttttCAATTTTTATAATTAATTGTTAGAGCAAATAAAAAACAACCCGAGCATTTTTTTAAAATGATTGTTTATTAAAATATATTTGTTTTTTATAATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGgt                                                                        <  1:3141735/138‑1 (MQ=255)

                                                                    ttttCCATTTTTATAATTATTGGGTAGCGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGgtgt                                                                      <  2:3407314/140‑1 (MQ=255)

                                                                    ttttACATTTTTCTAATTAATTGGTAGATCCAATAAAAATCAACACGTGCTTTTTTGTAAAATTATTGTTGATTAAAATATAAGTTTTTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGgtgt                                                                      <  2:442760/140‑1 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:1004747/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:1426900/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  2:1660113/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:44747/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  2:3952754/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATAACGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                    >  1:2666870/1‑140 (MQ=255)

                                                                       tcaaaTTTTCTAATTAATTGTTAGACCCAATCAAAATCAACACGAGCATTTTTTTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGt                                                                   <  2:3722072/137‑1 (MQ=255)

                                                                               tataattattTGTTATAGCACCTAAACATCACCCCCAGCCTTTTGGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgttg                                                           <  2:2085409/138‑1 (MQ=255)

                                                                               tCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgttg                                                           <  2:786086/140‑1 (MQ=255)

                                                                               tCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAACATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgttg                                                           <  2:664890/140‑1 (MQ=255)

                                                                               tCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTAGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCtagttg                                                           >  1:3860860/1‑140 (MQ=255)

                                                                                cTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGc                                                          <  2:1186789/140‑1 (MQ=255)

                                                                                        ttGTTAGACCCAATCAAAAACACCACGCGCCTTTTTTTAAAATGATTGTTGATAAAAACATACGTGATTTTTAACACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttg                                                  <  1:3433385/140‑1 (MQ=255)

                                                                                           gTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttgttg                                               >  2:920847/1‑140 (MQ=255)

                                                                                           gTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttgttg                                               >  2:1017844/1‑140 (MQ=255)

                                                                                           gTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttgttg                                               >  1:3684891/1‑140 (MQ=255)

                                                                                            tAGAGCAACAAAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTCATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGc                                              <  2:4828093/140‑1 (MQ=255)

                                                                                             atacccAATCACAACCAACACGCGCATTTTTTTAAAATTAATGTTTATAAAAACATCAGTTATTTTTATACTACAACGACCCTATACCGACTGAAGAAAAAACGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  1:1493855/136‑1 (MQ=255)

                                                                                             agagCCACTCAAAATCAACACGCGCTTTTTTTTAAAATGATTTTTGATTAAAAGATAGGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  1:1321403/140‑1 (MQ=255)

                                                                                             agagCCACTAAAAATAACCCCCAGCGTTTTTTTAAAATGAATGTTTTTTAAAACATAATTTTTTTTTTTCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                             <  1:4330560/140‑1 (MQ=255)

                                                                                                 ccACTAAAAATCAACACGAGCCTTTTTTTAAAATGAATGTTGATTAAAACATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACt                                         <  2:3772554/140‑1 (MQ=255)

                                                                                                    cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa                                      >  2:3645056/1‑140 (MQ=255)

                                                                                                    cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa                                      >  2:281059/1‑140 (MQ=255)

                                                                                                     tCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCaa                                     >  1:2553431/1‑140 (MQ=255)

                                                                                                         aaTCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATa                                 >  1:3984458/1‑140 (MQ=255)

                                                                                                         aaTCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTGGTTGCTCTTCTTGTTGCATACTTCAAAATa                                 >  1:4779005/1‑140 (MQ=255)

                                                                                                           taaaCACGACCCTTTTTTTAAATTTAATGTTGATTAAAAGATAAGTGTTTTTTATCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                               <  1:1710107/138‑1 (MQ=255)

                                                                                                           tCAACCCGAGCCTTTTTTTAAAATTATTGTTGTTTAAAAGATAATGGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                               <  1:4046468/140‑1 (MQ=255)

                                                                                                           acAACACGAGCTTTTTTGTAAAATTATTTTTGATTAAAATATAATTTATTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                               <  1:4111793/139‑1 (MQ=255)

                                                                                                              acacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGGTCTTCTTGTTGCATACTTCAAAATAGCCTg                            >  1:4291883/1‑140 (MQ=255)

                                                                                                              acacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTg                            >  1:1825690/1‑140 (MQ=255)

                                                                                                               cacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGt                           >  1:3989926/1‑140 (MQ=255)

                                                                                                               cacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGt                           >  1:294397/1‑140 (MQ=255)

                                                                                                                         tttGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTgc                 >  2:2297612/1‑140 (MQ=255)

                                                                                                                               aaaTGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtttt           >  2:1048149/1‑140 (MQ=255)

                                                                                                                                aatgaatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCttttt          >  1:249523/1‑140 (MQ=255)

                                                                                                                                aatgaatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCttttt          >  1:2332995/1‑140 (MQ=255)

                                                                                                                                   gactgTTTATTAAAACATAAGTCATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtttttttt       <  1:2411925/137‑1 (MQ=255)

                                                                                                                                    aatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGACTGTTGAAAGTTGCGCTTTTTTTTc      >  2:3902134/1‑140 (MQ=255)

AGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTT  >  NZ_CP034943/3280239‑3280514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`