Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,496,387 G→T 9.2% S347R (AGC→AGA)  EO946_RS17995 ← glucosaminidase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,496,3870GT 9.2% 568.8 / 10.3 237S347R (AGC→AGA) EO946_RS17995glucosaminidase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (57/149);  new base T (28/2);  total (86/151)
Fisher's exact test for biased strand distribution p-value = 3.13e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

CTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTG  >  NZ_CP034943/3496248‑3496525
                                                                                                                                           |                                                                                                                                          
       tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                                     <  2:777197/140‑1 (MQ=255)
       tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                                     <  1:1722255/140‑1 (MQ=255)
       tgttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATg                                                                                                                                     <  1:3523971/140‑1 (MQ=255)
        gttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGa                                                                                                                                    <  1:901657/140‑1 (MQ=255)
        gttCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGa                                                                                                                                    <  1:2434031/140‑1 (MQ=255)
              gTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTgg                                                                                                                                        <  1:3363313/130‑1 (MQ=255)
                tGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAt                                                                                                                            <  2:1956413/140‑1 (MQ=255)
                tGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAt                                                                                                                            <  2:3718545/140‑1 (MQ=255)
                tGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAt                                                                                                                            <  2:4477443/140‑1 (MQ=255)
                                     ttttttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaaga                                                                                                       <  2:2239299/140‑1 (MQ=255)
                                     ttttttCTGAGAAAAGAAGGCTGCGGCTGTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATTGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTACTGCCATTTTTTCTTTTTaaaga                                                                                                       <  2:339897/140‑1 (MQ=255)
                                     tttgttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTaaaga                                                                                                       <  1:4764880/140‑1 (MQ=255)
                                        tttCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTGTTTTTTTTTGTGGTTGTCGTGTTAATCCAATTTTTTTTTTTaaataaag                                                                                                    >  1:2328685/1‑140 (MQ=255)
                                           cTGAGAATAGCAGGCTGCGGCTGTGTTTGCGTGCTGCTCGCCGAAAATGTGCGCGGTCGTTATGGGCTCGGCTGTAGACATGGTCGTTTTGTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAgtgt                                                                                                 <  2:651103/140‑1 (MQ=255)
                                              agaAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGGTTGTGGATTAAGTTTAATGCCATTTTTTATTTTTTAATAAAGTGTGTa                                                                                              >  1:3172640/1‑140 (MQ=255)
                                                      agGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGc                                                                                      <  2:3648713/140‑1 (MQ=255)
                                                        gCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATTCTGGATGACGTGTAATTCCATTTTTTATTTTTAAAGAAATTGTGTATCTTTTGCAg                                                                                    >  2:3574181/1‑140 (MQ=255)
                                                             ggCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAgctgct                                                                               <  2:3229488/140‑1 (MQ=255)
                                                               cTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGt                                                                             <  2:4067040/140‑1 (MQ=255)
                                                                   gtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCg                                                                         <  1:4823826/140‑1 (MQ=255)
                                                                       gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                                     <  1:1177492/140‑1 (MQ=255)
                                                                         ttgctGCTCGCCTCAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                                   <  2:4185179/140‑1 (MQ=255)
                                                                         ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGGTGCTGTTTCGTTAAcc                                                                   <  1:2103309/140‑1 (MQ=255)
                                                                           gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                 <  2:498141/140‑1 (MQ=255)
                                                                           gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                 <  2:2723737/140‑1 (MQ=255)
                                                                           gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                 <  2:537832/140‑1 (MQ=255)
                                                                           gctgctCGCCTAAAATCTGCGCCGTCGTTAGTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                 <  1:2863996/140‑1 (MQ=255)
                                                                             tgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCtt                                                               <  2:574304/140‑1 (MQ=255)
                                                                              gctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGATGGATTACGGGTAAATACAATTTTTATTTTTAAATAAAGTGTCTATCTATTTCAGCGGCTGTTTAGTTaaaaccgtc                                                              >  2:4782539/1‑133 (MQ=255)
                                                                                tCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCat                                                            <  2:885472/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGCTGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                         <  1:2494028/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                         <  2:3774868/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                         <  1:1332049/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                         <  2:240162/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                         <  1:129042/140‑1 (MQ=255)
                                                                                   ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                         <  1:2620902/140‑1 (MQ=255)
                                                                                    gtAAAATATGCGGCGTGTTTTTTGTATATTATTTATACATGGTCGTTTTTATGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTATAGAAAGTGTCTATCTTTTGGAGCTGCTGGTTCGTTAACCCCTTCATATTc                                                        <  2:2567719/139‑1 (MQ=255)
                                                                                    ctacactCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTACAGCCATTTTTACTTGTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  1:1094512/134‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCTTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  2:4645143/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  1:4386430/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  2:3159322/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  1:1701977/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  1:1989349/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  1:3890608/140‑1 (MQ=255)
                                                                                    cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGAAATTGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                        <  1:360140/140‑1 (MQ=255)
                                                                                     tacaaTCTGCGCCGTCGTTATTGGATAGGCGGTAGACATGGTCGTTTTTTTGCTGCTGGAGGACGTGTAATGCCATTTTTTCTTTTTACAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                       <  2:2547874/137‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGTCATGGTCGTTTTTTTGATGCTGGATGAGGTGTTATGCCTTTTTTTCTTTTTAATGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTTACCCCTTGATATTCa                                                       <  2:1030034/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                       <  2:1874224/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                       <  2:1921689/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                       <  1:4076845/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                       <  2:221951/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                       <  1:1063336/140‑1 (MQ=255)
                                                                                     tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                       <  1:460489/140‑1 (MQ=255)
                                                                                      caaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                      <  1:2390320/139‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATTCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                      <  2:4140878/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                      <  1:3027211/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                      <  1:4104426/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                      <  2:3353514/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                      <  1:4264620/140‑1 (MQ=255)
                                                                                      aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTGTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                      <  1:2769738/140‑1 (MQ=255)
                                                                                            tGCGCCGTCGTTATTGGATAGGCTGTAGACCTGGTCGTTTTTTTGTTGCTGGATGTCGTGTAATGCCATTTTTTCTTTTTAAACAAAGTGTCTATCTTTTGCAGCTGCTGTTTGGTTAACCCCATCATATTCACTGtata                                                >  2:2166891/1‑140 (MQ=255)
                                                                                            tGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTTTGCTGGATGACGTGTAATTCCATTTTTTGTTTTTAAAGAAATTGTGTATCTTTTTCAGCTGCTGTTTAGTTAAACCCATGATATTCAAGGtata                                                >  2:1340219/1‑140 (MQ=255)
                                                                                              cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTATTCTGGATTACGTGTAATTGCATTTTTTGTTTTTTAATAAAGTTTATATAGTTTTGAGCTGCTGTTTGGTTAAAACCTTAATATTGAATGTatatt                                              >  2:3886752/1‑139 (MQ=255)
                                                                                              cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGGTGGATGACGTGTAAATCCATTTTTTATTTTTAAAGAAACTGTCTATATTTTGCAGGTGCTGTTTAGTTAAACCCATCATATTAACGGTatatt                                              >  2:1168663/1‑139 (MQ=255)
                                                                                              cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTGTTTTTGTTTCTGGGTGACGGGTAATTCCCTTTTTTTTTTTTAAAGAAAATTTGTTTGTTTTTGAGCTGGTGGTTGGTTAAACCCATAATAATCACTGTatatt                                              >  1:2517850/1‑139 (MQ=255)
                                                                                                ccGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatgat                                            <  2:2328685/140‑1 (MQ=255)
                                                                                                   tcgtTATGGGAGAGGCTGTAGACAGGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGAtgtg                                         <  1:4029640/140‑1 (MQ=255)
                                                                                                              tAGGCTGTATACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                              <  2:4372262/140‑1 (MQ=255)
                                                                                                              tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCGATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                              <  2:3182024/140‑1 (MQ=255)
                                                                                                              tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                              <  1:3282821/140‑1 (MQ=255)
                                                                                                              tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                              <  1:4964475/140‑1 (MQ=255)
                                                                                                              caGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                              <  1:3801917/139‑1 (MQ=255)
                                                                                                                 gCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTTCAGCTGCTGTTTCGTTAACCCCATCATATTCACTGTATATGATGTGGTTTTGTGAACCTg                           >  2:3544535/1‑140 (MQ=255)
                                                                                                                 gCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAAGCCATTTTTTCTTTTTAAATAAAGTGTCTATGTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATTTTGTTTTGTCAACCTg                           >  2:2508221/1‑140 (MQ=255)
                                                                                                                 gCTGTAGACATGGTCGTTTTTTTGATGCTGGAAGACGTTTAAAGCCCATTTTTCTTTTTAAAGAAATTGTGTATGTTTTGCAGCTGCTGTTTGGTTAACCCCTTAATATTAACGGGATATTATGTGATTTTGTGAAACTg                           >  2:3039368/1‑140 (MQ=255)
                                                                                                                      aGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCACTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCCTATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGAt                      <  1:508474/140‑1 (MQ=255)
                                                                                                                      aGACATGGTCGTGTTTTTTATTCTGGATTACGGGTAATGCCATTTTTTATTTTTAAAGAAATTGTGTATCTTTTGCAGCTGCTGTTTCGTTAAACCCGTAAAATTAACGGTATATGATTTTGTTTTTTTAAACTGTTGAt                      >  2:4308250/1‑140 (MQ=255)
                                                                                                                        aaaTGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa                    <  1:4870793/138‑1 (MQ=255)
                                                                                                                        aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa                    <  1:694688/140‑1 (MQ=255)
                                                                                                                        aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa                    <  2:3079892/140‑1 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTTATTCTGGATTACGTGTAAAGCCATTTTTTATTTTTTAATAAAGTGTCTATCTTTTGCAGCTGGTGTTTAGTTAAACCCTTAATATTAAATGTATATGATGTGATTTTGTGAACCTGTTGATAAATg                 >  2:457538/1‑140 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTTATGCTGGATGACGTGTAATGCCCTTTTTTCTTTTTAAATAAAGTGTCTATCTTTTGCACCTGCTGTTTCGTTAACCCCATAATATTAACTGTATATGATGTGCTTTTGTCAACCAGTTGATaaaag                 >  2:4410734/1‑137 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTTATGCTGGAGTACGTGTAATTCCATTTTTTATTTTTTAATAAAGTGTCTATATTTTGGAGCAGCTGTTTCGTTAAAACCATCATATTAACTGGATATGATTTGGTTTTGTCAACCTGTTGAttaatg                 >  2:1756199/1‑135 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTGATGCTGGATGACGTGTTATGCCAATTTTTATTTTTAAAGAAACTGTCTATCTTTTTCCGCTGCTTTTTGGTTAACCCCATCAAATTCACTGTATATTATGTGCTTTTGTCAAACTGTTGATAACTg                 >  2:513334/1‑140 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTGATGCTGGATGACGTGTAATTCCCAATTTTCTTTTTAAAGTAAGTGTCTATCTTTTGCCGCTGGTGTTTCGTTTACCCCGTCATATTCACTGTATATGATGTGGTTTTGTGAACCTGTTGTTAAATg                 >  2:2430961/1‑140 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTg                 <  1:1623964/140‑1 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTg                 <  1:621053/140‑1 (MQ=255)
                                                                                                                           tGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTATTTTTTAATACAGTGTGTATCTTTTGCAGCTGCTGTTTGGTTAACCCCGTCATATTCACGGTATATGATGTGGTTTTGTGAACCGGTTGATAAATg                 >  2:293585/1‑140 (MQ=255)
                                                                                                                            ggTCGTTTTTTTTATGCTGGATGACGTGTAATGGCCTTTTTTGTTTTTAAATAAATTGTCTATATTTTTCAGCTGCTTTTTAGTTAAACCCATAAAATTAAATGTATATTATGTGGTTTTTTCAACCTGTTTATAAATGc                >  2:1076065/1‑140 (MQ=255)
                                                                                                                            ggTCGTTTTTTTTAATCTGGCTGTCGTGTAATTCCATTTTTTCTTTTTAAATAAATTGTCTATCTTTTTCAGCAGCTGTTTAGTTAACCCCATCAAAATAAAATTATATGAAGTGCTTTTTTAAAACTTTTTATaaaaga                >  2:2587805/1‑136 (MQ=255)
                                                                                                                            ggTCGTTTTTTTGATTGTGGATGACGTGTAATGCCATTTTTTGTTTTTAAAAAAAGTGTGTTTCGTTTGCAGCTGCTGTTTAGTTTAACCCTTAATATTAAATTGATATTATGTTGTTTTGTAAAACTGTTGTTAACTGc                >  2:2854911/1‑140 (MQ=255)
                                                                                                                            ggTCGTTTTTTTGATGCTGGTTTGCGTGTAAAGCCAATTTTTATTTTTTAATAAAGTGTATATATTTTTCAGCGGCAGTTTGGTTAACCCCGTCATATTCACAGTAAATTATGTGGTTTTGTAAAACTGTTGATaaattc                >  2:132674/1‑136 (MQ=255)
                                                                                                                            ggTCGTTTTTTTGATGCTGGATTAAGTGTAATGGCATTTTTTGTTTTTAAATAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAACCCTTAAAATTCACTGTATATGAATTGCTTTTTTAAACCAGTTGATAACTGc                >  2:146288/1‑140 (MQ=255)
                                                                                                                             gTCGTTTTTTTTATGCTGGATGACGTGTAATGCCATTTTTTATTTTTAAATAAACTGTCTATCTTTTTCAGCTGCTGTTTCGTTAACCCCATCAAATTAACTTTATATTATTTGATTTTTTAAACCTTTTTATaaaatcc               >  2:4940348/1‑135 (MQ=255)
                                                                                                                             gTCGTTTTTTTGATTCTGGATGACGTGTAATGCCATTTTTTGTTTTTATATAAAGTGTGTATAGTTTTCGGGGGCTGTTTCGTTAAAACCCTAAAATTTACTGTATATGATGTGCGTTTTTGAACCTGTTGTTAACAGcc               >  2:1570261/1‑140 (MQ=255)
                                                                                                                             gTCGTTTTTTTGATTCTGGATGACGTGTAATGCCATTTTTTATTTTTAAAGAAAGTGTCTATCTTTTTCAGCTGCTGTTTCGTTAACCCCGTCATATTAAATGTTTATGATTTGCTTTTGTCAAACTGTTGATaaattcc               >  2:4256359/1‑135 (MQ=255)
                                                                                                                             gTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAATAAAGTGTCTATCTTTTTCAGCGGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGcc               >  2:4867493/1‑140 (MQ=255)
                                                                                                                               cGTTTTTTTGATGCTGGATGACGTGTAATTCCATTTTTTATTTTTAAATAAAGTGTCTATAATTTTCAGCTGCTGTTTCGTTAACCCCTTCATATTAACTGTATATTATGTGCTTTTTTAAACCTGTTGATAACTGCCTg             >  2:4692688/1‑140 (MQ=255)
                                                                                                                                     tttGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGc       <  2:140251/140‑1 (MQ=255)
                                                                                                                                     tgtGATGCTGGATGACGTGTAATGCCATTTTTTCTTTGTACAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATAGGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGAGACGc       <  2:4392297/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTGCTGTTGTTCCAGTTGCTTTTTTCGTTGCCTTTACTTTTTTCTGAGAAAAGAAGGCTGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTG  >  NZ_CP034943/3496248‑3496525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑