Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,584,291 G→T 5.5% S324S (TCG→TCT EO946_RS18420 → N‑acetylneuraminate synthase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,584,2910GT 5.5% 1290.8 / 12.0 489S324S (TCG→TCTEO946_RS18420N‑acetylneuraminate synthase family protein
Reads supporting (aligned to +/- strand):  ref base G (196/246);  new base T (2/45);  total (198/291)
Fisher's exact test for biased strand distribution p-value = 7.03e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 491 total aligned reads displayed.

TGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTT  >  NZ_CP034943/3584152‑3584428
                                                                                                                                           |                                                                                                                                         
tgACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCg                                                                                                                                           >  1:3138889/1‑140 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAATCTCCAAAAGATGCGGAAATGAGAATGCATTAATTCATTTAAAACCCCAGGACTAATTTACTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                          >  2:2856597/1‑140 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                          >  2:2422045/1‑140 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                          >  2:3584868/1‑140 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                          >  2:2998897/1‑140 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                          <  1:1797633/140‑1 (MQ=255)
 gACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGt                                                                                                                                          <  1:4833862/140‑1 (MQ=255)
      ccAACCACAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAAc                                                                                                                                     <  1:2514747/140‑1 (MQ=255)
         aCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATTCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                                  <  2:4734007/140‑1 (MQ=255)
         aCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                                  >  2:1781084/1‑140 (MQ=255)
         aCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                                  <  1:1936968/140‑1 (MQ=255)
          ccAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGc                                                                                                                                 <  2:1383155/140‑1 (MQ=255)
                  taAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTacc                                                                                                                         >  2:4139954/1‑139 (MQ=255)
                                          tGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGa                                                                                                 >  2:2586496/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                           <  1:1050524/128‑1 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                               >  1:2477923/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                               >  2:4932221/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAAt                                                                                               >  2:2774014/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATGTATCGTTAAACCAGCCCATTACACCCAACGAAATCAAAAGTCTAAGAAt                                                                                               >  1:2813833/1‑140 (MQ=255)
                                            aaTAAAACAAGCTCCAAAAGAGGCGGAAATGAGAATGCATTAATGCCTTTACAATCCGACGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGACCATTACAACCAACGAAATAAAAAGTCTAAGAAt                                                                                               >  2:2568073/1‑140 (MQ=255)
                                                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                          <  1:2683155/140‑1 (MQ=255)
                                                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                          >  1:100827/1‑140 (MQ=255)
                                                    caGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                       <  2:3179036/139‑1 (MQ=255)
                                                    aaGCTCCAAAAGCGGCGGAAATGAGAATGCAATAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                       <  1:82431/140‑1 (MQ=255)
                                                    aaGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                       <  2:3773181/140‑1 (MQ=255)
                                                      gCTCCAAAAGCTGCGGAAATGAGAATGCATGAATGCCTTTACAACCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAACCCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     <  1:4593107/140‑1 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATCAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:4295800/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAATTCTAAGAATTGCGATTGCa                                                                                     >  1:95772/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  2:1902774/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:441827/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:4074667/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:4522791/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:1646990/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:2904730/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:204618/1‑140 (MQ=255)
                                                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                     >  1:2349381/1‑140 (MQ=255)
                                                        tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                   <  1:1527348/140‑1 (MQ=255)
                                                        tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                   <  1:143039/140‑1 (MQ=255)
                                                         ccAAAAGATGCGTAATTGAGAATGCATTAATGCCTTTACAAGCCGAGGAGGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTAAACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                  <  2:1585574/140‑1 (MQ=255)
                                                         ccAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                  <  1:17095/140‑1 (MQ=255)
                                                           aaaaGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGat                                                                                >  2:2695724/1‑140 (MQ=255)
                                                              cgATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACTCCCACCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                             <  2:3672524/139‑1 (MQ=255)
                                                              aGATGCGGAAATGAGACTGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                             <  2:201645/140‑1 (MQ=255)
                                                                  gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                         >  1:3894386/1‑140 (MQ=255)
                                                                  gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                         >  1:597325/1‑140 (MQ=255)
                                                                  gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                         >  1:3176899/1‑140 (MQ=255)
                                                                  gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                         >  1:2950528/1‑140 (MQ=255)
                                                                                           tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTCAACTGATATGTAGGAAAAAATCTACTAGTCTGCaaaaaa                                                >  2:2928383/1‑140 (MQ=255)
                                                                                            ttACAGGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATTTATGCTAAAATCGACTCGTCTGCCGaaaaa                                               >  2:3958756/1‑140 (MQ=255)
                                                                                                aaGCCGAGGACGCTTTTCCTAATGTAGCAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTACGAATTGCGATTGCATTTATTTGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                           <  1:2583782/140‑1 (MQ=255)
                                                                                                aaGCCGAGGACGCTTTTCCTAAAGTAGAAACTGATTTTTTATCGTTAAACCAGCCCTTTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                           <  1:1150434/140‑1 (MQ=255)
                                                                                                aaGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                           <  2:3453805/140‑1 (MQ=255)
                                                                                                aaGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                           <  1:3584868/140‑1 (MQ=255)
                                                                                                   ccGTGGACGATTTTCCTAAAGTAGAAACGGCTTTTTTATCGTTAAACCACCCAATTACACCCAACGAAATAAAACGTCTAATATTGGCTTTTGCAGTTATTGGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCgctg                                        <  1:2113752/140‑1 (MQ=255)
                                                                                                    cGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                       >  2:1126458/1‑140 (MQ=255)
                                                                                                      agcaCGCATTTCCTAAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAATAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                     <  2:4463542/137‑1 (MQ=255)
                                                                                                      aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTCCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAATCGCTGCTc                                     >  2:2109944/1‑140 (MQ=255)
                                                                                                       ggACTCATTTCCTCAAGTAGAAACTGCTGTTTTATCTTTAAACCAGCCCATTCCACCCAACGAAATAAAACGTCTAAGAATTGCGTTTGAAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                    <  1:3649270/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCTCAATTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCACCCAAATAAAAAGACTACGAATTGCGATTGCAGTGATTTGTAGGCAAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                    <  2:4670724/140‑1 (MQ=255)
                                                                                                       ggACGCATTTCCGAAAGTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                    <  2:1460565/140‑1 (MQ=255)
                                                                                                       gcaCGCATTTCATAAAGTAGAAAGTGCTGTTTTAACTTTAAACCAGCCAATTAAACCAAACGAAATAAAAAGTCTAAGAATTCCTATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                    <  1:3318436/138‑1 (MQ=255)
                                                                                                           taaTTTCCTAAAGTACCAACTGATGATTTATCTTTAAACCACCCCATTACACCAAACGAAATAAAAAGTCTAATAATTGCGTTTTCATTTATATGTAGGATAAAATCGACTCGTCTACCGAAAAAAGCGCTGCTCCCTAt                                <  1:1281064/138‑1 (MQ=255)
                                                                                                           gattttCCTCAATTTTCAAGTGCTGTTTTATGGTTAAACCAGCCCATTACACCCAACGAAATAAATTTTCTAATATTTGCGTTTGCAGTTTTATGTGGGATAAAATGGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                <  1:88190/137‑1 (MQ=255)
                                                                                                           gCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACTAAATAAAACGTCTAAGATTTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                <  2:2072763/140‑1 (MQ=255)
                                                                                                            cATTTCCTCAAGTAGCAACTGCTGTTTTATCGTTAAACCCCCCCATTACCCCCAACTAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                               <  2:4692303/140‑1 (MQ=255)
                                                                                                            cATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGAGCTTCTCCCTAtt                               >  2:1082587/1‑140 (MQ=255)
                                                                                                              tttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAACGTATAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                             <  1:3714003/140‑1 (MQ=255)
                                                                                                               ttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCCCCCCATTACACCCAACCAAATAAAACGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                            <  1:4781660/140‑1 (MQ=255)
                                                                                                               ttCCTAAAGTAGCAACGGCTGATTTATCTTTAAACCCGCCCATCACACCCACGGAAATAAAAAGTCTACGAATGGCGTTTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                            <  1:1912306/140‑1 (MQ=255)
                                                                                                                tCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCACCCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACg                           <  2:1719797/140‑1 (MQ=255)
                                                                                                                    cAAGGAGAAACTGCTGATTTATCGTTAAACCAGCCCATTACCCCAAACGAAATAAAACGGCAAAGAATTGCGATGGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtat                       <  2:1307678/140‑1 (MQ=255)
                                                                                                                    aaaGTAGCAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtat                       <  2:1521577/139‑1 (MQ=255)
                                                                                                                    aaaGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAATTAAACCGTCTAAGAATTGGTATGGAAGTGATATGTTGGCTAAAATCGACTCGTCTGCCTAAAAAAGCGATGATCCCTATTTACGGtat                       <  2:2513473/139‑1 (MQ=255)
                                                                                                                    aaaGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtat                       <  2:335581/139‑1 (MQ=255)
                                                                                                                     aaGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtata                      >  2:848195/1‑140 (MQ=255)
                                                                                                                     aaGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtata                      >  2:89342/1‑140 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCCCCCCATAACACCCAACGAAATAAAACGTCTAATAATTGCGTTTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                    <  1:3272447/140‑1 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCTTCTGCCGAAAAAAGCGCTGCTCCCTAGTTACGGTATAcc                    >  2:18351/1‑140 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                         <  2:3109629/135‑1 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                         >  1:3109629/1‑135 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                         >  1:2986386/1‑135 (MQ=255)
                                                                                                                       gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                    <  2:777195/140‑1 (MQ=255)
                                                                                                                         aGCAACTGATCTTTTATCGATAAACCCCCCCATTACACCCAACCAAATAAAAAGAATAATAATTGCTTTTGCAGTGTTATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt                  <  1:4139954/140‑1 (MQ=255)
                                                                                                                           cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                             <  2:4829601/79‑1 (MQ=255)
                                                                                                                           cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCt                >  2:3151849/1‑140 (MQ=255)
                                                                                                                           cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCt                >  2:2292359/1‑140 (MQ=255)
                                                                                                                            aaCTGCTGATTTATCGTTAAACCAGCCCATTACACCCACCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTg               <  1:1864467/140‑1 (MQ=255)
                                                                                                                             aCTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAAGGTATACCATCTGt              >  2:2207114/1‑140 (MQ=255)
                                                                                                                                 cttaTTTATCGTTAAACCAGCCCATCACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa          <  1:1098894/137‑1 (MQ=255)
                                                                                                                                 ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                    >  1:3988456/1‑114 (MQ=255)
                                                                                                                                   gATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACg        <  2:712177/140‑1 (MQ=255)
                                                                                                                                   gATTTATCTTTAAACCACCCCATTACACCCACCGAAAAAAAAAGTATACGAATTGCGATTGCAGTGATATGTTGGATAACAGAGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACg        <  1:69364/140‑1 (MQ=255)
                                                                                                                                   gATTTATCGTTAAACCAGCACATTACCCCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACg        <  2:1202435/140‑1 (MQ=255)
                                                                                                                                     tttATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCt      <  2:4405302/140‑1 (MQ=255)
                                                                                                                                     tttATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCt      <  2:630206/140‑1 (MQ=255)
                                                                                                                                     tttATCGTTAAACCACCCCATTCCACCCAACGAAATAAAAAGTCTAAGACTTCCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCt      <  1:4674077/140‑1 (MQ=255)
                                                                                                                                         tCGTTACACCAGCCCATGACACCCCACGAAATAAACACTCTACGAATTGCGATTGCAGTGAAATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATCTACGGTATACCATCTGTTGAACGCTGttt  <  2:2456862/140‑1 (MQ=255)
                                                                                                                                         tCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                    <  2:4264914/122‑1 (MQ=255)
                                                                                                                                         tCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGttt  <  2:4057938/140‑1 (MQ=255)
                                                                                                                                         tCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGttt  >  1:3081345/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTT  >  NZ_CP034943/3584152‑3584428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: