Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,584,300 G→C 7.0% Q327H (CAG→CAC EO946_RS18420 → N‑acetylneuraminate synthase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,584,3000GC 7.0% 1107.8 / 10.6 437Q327H (CAG→CACEO946_RS18420N‑acetylneuraminate synthase family protein
Reads supporting (aligned to +/- strand):  ref base G (174/219);  new base C (3/38);  total (178/259)
Fisher's exact test for biased strand distribution p-value = 1.09e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 461 total aligned reads displayed.

ACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCC  >  NZ_CP034943/3584161‑3584439
                                                                                                                                           |                                                                                                                                           
aCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATTCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                                             <  2:4734007/140‑1 (MQ=255)
aCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                                             <  2:4207125/140‑1 (MQ=255)
            aaaaaGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACAccc                                                                                                                                 <  2:3327827/140‑1 (MQ=255)
               aaGCAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAAc                                                                                                                              <  1:2563153/140‑1 (MQ=255)
                                 tGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAACAAGTCTAAGa                                                                                                            <  2:4691826/140‑1 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAACTGAGAATGGATTAATGCCTTTACAATCCGAGCACGCATATCATCAAGTAGCAACTGATGATTTATCGTTAAACCAGCCCATGACAACCAACGAAATAAAAAGTCAAAGAAt                                                                                                          >  2:2763518/1‑140 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                          >  1:738439/1‑140 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                          >  2:2080442/1‑140 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                          >  2:1093958/1‑140 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                          >  1:2467974/1‑140 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                          >  1:2148113/1‑140 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                          >  2:4932221/1‑140 (MQ=255)
                                   aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATGTATCGTTAAACCAGCCCATTACACCCAACGAAATCAAAAGTCTAAGAAt                                                                                                          >  1:2813833/1‑140 (MQ=255)
                                        aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                     >  1:100827/1‑140 (MQ=255)
                                        aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                     >  2:3724128/1‑140 (MQ=255)
                                        aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                     >  2:238467/1‑140 (MQ=255)
                                           aaGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                                  <  1:1803119/140‑1 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                <  1:3724128/140‑1 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  2:2113752/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:3224687/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  2:1044692/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:4074667/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:4098070/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:4405302/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:4522791/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:630206/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:2904730/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:2349381/1‑140 (MQ=255)
                                             gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                >  1:1646990/1‑140 (MQ=255)
                                               tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                              <  1:143039/140‑1 (MQ=255)
                                               tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                              <  1:3942082/140‑1 (MQ=255)
                                                ccAACAGAGGCGCAAATGAGAATGCATTACTGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCGCATTACACCCAACGAAAGAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                             <  2:4037825/140‑1 (MQ=255)
                                                ccAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                             <  1:1093958/140‑1 (MQ=255)
                                                ccAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                             <  1:17095/140‑1 (MQ=255)
                                                  aaaaGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCAGTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGat                                                                                           >  2:465307/1‑140 (MQ=255)
                                                     aGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                                        <  1:191489/140‑1 (MQ=255)
                                                         gCGGAAATGAGAATGCATTAATGCCTTTGCAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCACACATATGTAgg                                                                                    >  1:3281672/1‑140 (MQ=255)
                                                         gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                                    >  1:2950528/1‑140 (MQ=255)
                                                         gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                                    >  1:1886711/1‑140 (MQ=255)
                                                         gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAAACACCCCAATACACCCAACGAAATAAAAAGTCAAAGAATTCCGATTGCACTGATATGTAgc                                                                                    >  2:4278649/1‑139 (MQ=255)
                                                                                  tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTCAACTGATATGTAGGAAAAAATCTACTAGTCTGCaaaaaa                                                           >  2:2928383/1‑140 (MQ=255)
                                                                                   ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCTTCTGCCGaaaaa                                                          >  2:2631751/1‑140 (MQ=255)
                                                                                   ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                                          >  2:1827373/1‑140 (MQ=255)
                                                                                   ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                                          >  1:4637548/1‑140 (MQ=255)
                                                                                       aaGCCGAGGACGCTTTTCCTAAAGTAGAAACTGATTTTTTATCGTTAAACCAGCCCTTTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                                      <  1:1150434/140‑1 (MQ=255)
                                                                                       aaGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                                      <  2:3903195/140‑1 (MQ=255)
                                                                                          ccGAGGACGCATTTCCTCAAGTAGCAACTGCTTATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAATGTCTAAGAATTGCGATTGAAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCgctg                                                   <  2:1733158/140‑1 (MQ=255)
                                                                                          ccGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctg                                                   <  1:3625553/140‑1 (MQ=255)
                                                                                          ccGAGCACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctg                                                   <  2:1301378/140‑1 (MQ=255)
                                                                                           cGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                                  >  2:1126458/1‑140 (MQ=255)
                                                                                             agtaCGAATTTCCTCTAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAATAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                                <  2:1404722/137‑1 (MQ=255)
                                                                                             agcaCGCATTTCCTAAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAATAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                                <  2:4463542/137‑1 (MQ=255)
                                                                                             aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGAAGTGATATGTAGGATAAAATCTACTAGTCTGACGAAAAAAGCGCTGCTc                                                >  2:2572271/1‑140 (MQ=255)
                                                                                              ggACGCATTTCCTCAAGTAGCAACTGCTGTTTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAACAGTCTAAGAATTGCTATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                               <  2:205805/140‑1 (MQ=255)
                                                                                              ggACGCATTTCCGAAAGTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                               <  2:1460565/140‑1 (MQ=255)
                                                                                                actcATTTACTCACTTAGCAACTGCTTATTTTTCGTTAAACCAGCCCATTACACCCAACGAATTAAAAAGTCTAAGAATTGCTATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCt                                             <  2:3032158/137‑1 (MQ=255)
                                                                                                  taaTTTCCTAAAGTACCAACTGATGATTTATCTTTAAACCACCCCATTACACCAAACGAAATAAAAAGTCTAATAATTGCGTTTTCATTTATATGTAGGATAAAATCGACTCGTCTACCGAAAAAAGCGCTGCTCCCTAt                                           <  1:1281064/138‑1 (MQ=255)
                                                                                                  gaaTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                           <  2:2549816/138‑1 (MQ=255)
                                                                                                  gaaTTTCCTAAAGTAGCATCTGCTTATTTATCTTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTGTAAGAATTGCGATTGCTGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                           <  1:1525840/138‑1 (MQ=255)
                                                                                                  gCATTTCCTCAAGGAGCAACTGCTTATTTATCGTTAAACCCGCCCATTACACCCAACGAAATAAAAAGTGTACGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                           <  2:3104319/140‑1 (MQ=255)
                                                                                                   cATTTCCTCAAGTAGCAACTGCTGATTTATCTTTAAACCCGCCCATTACACCCACCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                                          <  1:1047269/140‑1 (MQ=255)
                                                                                                   cATTTCCTCAAGTACAAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                                          <  2:3581600/140‑1 (MQ=255)
                                                                                                   attttCATCAATTAGAAACTGCTTATTTATCGTTAAACCAGCCAATTACACCCAACGAAAAAAAAAGTCTAAGAATTGCGTTTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                                          <  1:2998897/138‑1 (MQ=255)
                                                                                                     tttCCTCATGTAGCAACTGCTTATTTATCGTTAAACCAGCCAATTACACCCAACTAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATTTGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                        <  2:575760/140‑1 (MQ=255)
                                                                                                     tttCCTAAATTAGCAAGTGCTGATTTATCTTTAAACCAGCCAATTACACCAAACTAAATAAAAAGTCTAAGAATTGCGTTTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                        <  2:697859/140‑1 (MQ=255)
                                                                                                     tttCCTAAATTAGAAATTTTTTATTTATCTTTAAACCAGCCAATTACACCCAACTAAATAAAAAGTATAAGATTTGCGTTTGCAGTGATTTGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                        <  1:2695724/140‑1 (MQ=255)
                                                                                                     tttCATAAATTAGAAAGTGCTTATTTATCTTTAAACCAGCCCATTACACCAAACGAAATAAAAAGTCTAATAATTGCTTTTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                        <  1:3113099/140‑1 (MQ=255)
                                                                                                      ttCCTCAAGTAGCAACTGCTTATTTAACTTTAAACCACCCAATTACCCCCAACGAAATAAAAAGTCTAAGAATGGGTATTGCTGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                       <  1:3357554/140‑1 (MQ=255)
                                                                                                      ttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCCCCCCATTACACCCAACCAAATAAAACGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                       <  1:4781660/140‑1 (MQ=255)
                                                                                                      ttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                       <  2:3669927/140‑1 (MQ=255)
                                                                                                      ttCCTCAAGGAGAAAGTGCTGATTTATCTTTAAACCCGCCAATTACACCCAACGAAAAAAAAAGACTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                       <  1:4587358/140‑1 (MQ=255)
                                                                                                      ttCCTAAAGTAGCAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                       <  1:3659143/140‑1 (MQ=255)
                                                                                                      ttCATCAAGTAGCAACTGGTGATTTATCGTTAAACCAGCCAATTACCCCCACCGAAATAAAAAGTCTACGAATTGCGATTGCAGTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                       <  1:4858614/140‑1 (MQ=255)
                                                                                                         cTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGACTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                                    <  2:2467974/140‑1 (MQ=255)
                                                                                                            aaGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtata                                 >  2:89342/1‑140 (MQ=255)
                                                                                                              gagacagctgcttATTTATCGTTAAACCCGCCAATTACACCAAACGAAATAAAAAGTATAAGAATTGCGATTGCAGTCATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                               <  2:18487/133‑1 (MQ=255)
                                                                                                              gTAGCAACTGCTGATTTATCGTTAAACCCCCCCATTACACCCAACTAAATAAAAAGTCTAAGAATTGCGATTGAAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                               <  2:3403464/140‑1 (MQ=255)
                                                                                                                aGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt                             <  2:4091057/140‑1 (MQ=255)
                                                                                                                  cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                                        <  2:4829601/79‑1 (MQ=255)
                                                                                                                  cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCt                           >  2:780058/1‑140 (MQ=255)
                                                                                                                    aCTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGt                         >  1:920307/1‑140 (MQ=255)
                                                                                                                    aCTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAAGGTATACCATCTGt                         >  2:2207114/1‑140 (MQ=255)
                                                                                                                        ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa                     >  2:3129400/1‑140 (MQ=255)
                                                                                                                        ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa                     <  2:3176899/140‑1 (MQ=255)
                                                                                                                         tgtttttTCGTTAAACCCGCCCATTACACCAAACGAAATAAAATGTATAAGACTTGGCATTGATTTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAAc                    <  1:3159224/137‑1 (MQ=255)
                                                                                                                         tgATTTATCGTTAAACCCGCCCATTACACCCACCGAAATAAAAAGTCTACGAAGTGCGATTGCAGTGAAAGGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAAc                    <  1:863611/140‑1 (MQ=255)
                                                                                                                         tgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATGGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAAc                    <  1:2080442/140‑1 (MQ=255)
                                                                                                                         tgATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAAc                    <  1:2005471/140‑1 (MQ=255)
                                                                                                                          taTTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACg                   <  2:2278120/139‑1 (MQ=255)
                                                                                                                          gATTTATCGTTAAACCAGCACATTACCCCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACg                   <  2:1202435/140‑1 (MQ=255)
                                                                                                                            tttATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCt                 <  2:4405302/140‑1 (MQ=255)
                                                                                                                              tATCGTTAAACCAGCCCATTCCACCAAACCAAATAAAAAGTATACGATTTGCGATTGCAGTCATATTTAGCCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTGACGGTATACCATCTGTTGAACGCTGt               <  2:1568890/140‑1 (MQ=255)
                                                                                                                              tATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGAAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGt               <  1:2586496/140‑1 (MQ=255)
                                                                                                                                tctttAAACCAGCCAATTACACCAAAACAAATAAAAAGTATAAGATTTGCTTTTTCAGTTATTTTTAGTCTAAAATCGACTCGTCTTCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGttt             <  2:1696332/137‑1 (MQ=255)
                                                                                                                                tCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                               >  1:4264914/1‑122 (MQ=255)
                                                                                                                                tCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGttt             >  1:3081345/1‑140 (MQ=255)
                                                                                                                                    tAAACCACCCCATTACACCCACCGAAATAAAAAGTATAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATc         <  1:3997134/140‑1 (MQ=255)
                                                                                                                                     aaaCCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTCTTTACGGTATACCATCTGATGAACGCTGTTTAATCa        >  2:853709/1‑140 (MQ=255)
                                                                                                                                           gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGcc  >  2:1907981/1‑140 (MQ=255)
                                                                                                                                           gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTAAACCATATGTTGAACGCTGTTTAATCAATTgac  >  2:2857068/1‑138 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCC  >  NZ_CP034943/3584161‑3584439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: